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    Intermediates and Methods for Forming Passivated Surfaces on Oxide Layers and Articles Produced Thereby
    1.
    发明申请
    Intermediates and Methods for Forming Passivated Surfaces on Oxide Layers and Articles Produced Thereby 有权
    中间体和氧化层上形成钝化表面的方法和由此产生的制品

    公开(公告)号：US20080176761A1

    公开(公告)日：2008-07-24

    申请号：US11943851

    申请日：2007-11-21

    申请人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    发明人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    IPC分类号： C40B50/14 ,  C40B40/00 ,  C07F9/09 ,  C07F9/02 ,  C40B50/18

    CPC分类号： G01N33/54393 ,  G01N33/551 ,  Y10T428/31678

    摘要： Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.

    摘要翻译： 描述了用于在氧化物层上形成钝化表面的中间体和方法以及由此制备的制品。 氧化物表面上的羟基或氢氧基与式Y（L-POL）m的金属试剂反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒 或胺，“Pol”表示钝化剂，例如聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸酯官能团的钝化剂或包含钝化部分和多个与Y形成复合物的多个官能团的多价试剂反应。钝化剂还可以包括官能团 例如生物素，以提供具有所需功能的表面。 被钝化的表面表现出与生物分子的最小结合，并可用于单分子检测方案。

    Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby
    2.
    发明授权
    Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby 有权
    用于在由其制造的氧化物层和制品上形成钝化表面的中间体和方法

    公开(公告)号：US07928038B2

    公开(公告)日：2011-04-19

    申请号：US11943851

    申请日：2007-11-21

    申请人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    发明人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    IPC分类号： C40B60/14 ,  C12M1/34 ,  C07H21/02 ,  A61K38/04

    CPC分类号： G01N33/54393 ,  G01N33/551 ,  Y10T428/31678

    摘要： Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.

    摘要翻译： 描述了用于在氧化物层上形成钝化表面的中间体和方法以及由此制备的制品。 氧化物表面上的羟基或氢氧基与式Y（L-Pol）m的金属试剂反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol “表示钝化剂，例如聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸酯官能团的钝化剂或包含钝化部分和多个与Y形成复合物的多个官能团的多价试剂反应。钝化剂还可以包括官能团 例如生物素，以提供具有所需功能的表面。 被钝化的表面表现出与生物分子的最小结合，并可用于单分子检测方案。

    Intermediates And Methods For Forming Passivated Surfaces On Oxide Layers And Articles Produced Thereby
    3.
    发明申请
    Intermediates And Methods For Forming Passivated Surfaces On Oxide Layers And Articles Produced Thereby 审中-公开
    中间体和方法在氧化物层和由此产生的制品上形成钝化表面

    公开(公告)号：US20110159305A1

    公开(公告)日：2011-06-30

    申请号：US13044759

    申请日：2011-03-10

    申请人： Steven M. MENCHEN ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    发明人： Steven M. MENCHEN ,  Christina E. Inman ,  Meng Taing ,  Khai Luong ,  Handong Li

    IPC分类号： B32B15/00

    CPC分类号： G01N33/54393 ,  G01N33/551 ,  Y10T428/31678

    摘要： Intermediates and methods for forming passivated surfaces on oxide layers and articles produced thereby are described. Hydroxyl or hydroxide groups on the oxide surfaces are reacted with a metal reagent of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a polyvalent reagent comprising a passivating moiety and a plurality of functional groups that are reactive with or that form complexes with Y. The passivating agent can also include a functional group such as biotin to provide surfaces with a desired functionality. The passivated surfaces exhibit minimal binding to bio-molecules and can be used in single-molecule detection schemes.

    摘要翻译： 描述了用于在氧化物层上形成钝化表面的中间体和方法以及由此制备的制品。 氧化物表面上的羟基或氢氧基与式Y（L-Pol）m的金属试剂反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol “表示钝化剂，例如聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸酯官能团的钝化剂或包含钝化部分和多个与Y形成复合物的多个官能团的多价试剂反应。钝化剂还可以包括官能团 例如生物素，以提供具有所需功能的表面。 被钝化的表面表现出与生物分子的最小结合，并可用于单分子检测方案。

    Deposition of metal oxides onto surfaces as an immobilization vehicle for carboxylated or phophated particles or polymers
    4.
    发明授权
    Deposition of metal oxides onto surfaces as an immobilization vehicle for carboxylated or phophated particles or polymers 有权
    将金属氧化物沉积在表面上，作为羧化或磷化颗粒或聚合物的固定载体

    公开(公告)号：US08173198B2

    公开(公告)日：2012-05-08

    申请号：US12508539

    申请日：2009-07-23

    申请人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  George A. Fry

    发明人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  George A. Fry

    IPC分类号： G01N1/31 ,  C23C4/10 ,  C12N11/00 ,  A61B5/055 ,  C07H21/02

    CPC分类号： C07F9/6596 ,  C07F9/093 ,  C12Q1/6834 ,  Y10S530/81

    摘要： Intermediates and methods for forming activated metal complexes bound to surfaces on oxide layers, immobilizing beads to the modified surface and articles produced thereby are described. Hydroxyl groups on the oxide surfaces are reacted with a metal reagent complex of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a methoxyethanol, a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a plurality of functional groups that are reactive with or that form complexes with Y. The metal oxide surfaces exhibit minimal binding to bio-molecules, exhibit uniform deposition and immobilization of beads at high density, can be subsequently modified to create surfaces having a variety of properties, and can be used for nucleic acid sequencing and other analyses and in single-molecule detection schemes.

    摘要翻译： 描述了形成与氧化物层上的表面结合的活化金属配合物的中间体和方法，将珠子固定在改性表面上以及由此制备的制品。 氧化物表面上的羟基与式Y（L-Pol）m的金属试剂络合物反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol” 代表钝化剂如甲氧基乙醇，聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸盐官能团或与Y形成复合物的多个官能团的钝化剂反应。金属氧化物表面表现出与生物分子的最小结合，表现出均匀的沉积和 可以随后修饰珠子以高密度的固定，以产生具有多种性质的表面，并可用于核酸测序和其他分析以及单分子检测方案。

    DEPOSITION OF METAL OXIDES ONTO SURFACES AS AN IMMOBILIZATION VEHICLE FOR CARBOXYLATED OR PHOPHATED PARTICLES OR POLYMERS
    5.
    发明申请
    DEPOSITION OF METAL OXIDES ONTO SURFACES AS AN IMMOBILIZATION VEHICLE FOR CARBOXYLATED OR PHOPHATED PARTICLES OR POLYMERS 有权
    将金属氧化物沉积在表面上作为用于羧基化或亲水化的颗粒或聚合物的固定化载体

    公开(公告)号：US20100086927A1

    公开(公告)日：2010-04-08

    申请号：US12508539

    申请日：2009-07-23

    申请人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  George A. Fry

    发明人： Steven M. Menchen ,  Christina E. Inman ,  Meng Taing ,  George A. Fry

    IPC分类号： C12Q1/68 ,  B05D1/36 ,  B05D3/06 ,  C07F7/00 ,  C07F7/02

    CPC分类号： C07F9/6596 ,  C07F9/093 ,  C12Q1/6834 ,  Y10S530/81

    摘要： Intermediates and methods for forming activated metal complexes bound to surfaces on oxide layers, immobilizing beads to the modified surface and articles produced thereby are described. Hydroxyl groups on the oxide surfaces are reacted with a metal reagent complex of the formula Y(L-Pol)m, where Y is a transition metal, magnesium or aluminum, L is oxygen, sulfur, selenium or an amine, and “Pol” represents a passivating agent such as a methoxyethanol, a polyethylene glycol, a hydrocarbon, or a fluorocarbon. The resulting modified surface can be further reacted with a passivating agent having a phosphate functional group or a plurality of functional groups that are reactive with or that form complexes with Y. The metal oxide surfaces exhibit minimal binding to bio-molecules, exhibit uniform deposition and immobilization of beads at high density, can be subsequently modified to create surfaces having a variety of properties, and can be used for nucleic acid sequencing and other analyses and in single-molecule detection schemes.

    摘要翻译： 描述了形成与氧化物层上的表面结合的活化金属配合物的中间体和方法，将珠子固定在改性表面上以及由此制备的制品。 氧化物表面上的羟基与式Y（L-Pol）m的金属试剂络合物反应，其中Y是过渡金属，镁或铝，L是氧，硫，硒或胺，“Pol” 代表钝化剂如甲氧基乙醇，聚乙二醇，烃或碳氟化合物。 所得到的改性表面可以进一步与具有磷酸盐官能团或与Y形成复合物的多个官能团的钝化剂反应。金属氧化物表面表现出与生物分子的最小结合，表现出均匀的沉积， 可以随后修饰珠子以高密度的固定，以产生具有多种性质的表面，并可用于核酸测序和其他分析以及单分子检测方案。

    SINGLE MOLECULE NUCLEIC ACID SEQUENCING USING MULTIPHOTON FLUORESCENCE EXCITATION
    8.
    发明申请
    SINGLE MOLECULE NUCLEIC ACID SEQUENCING USING MULTIPHOTON FLUORESCENCE EXCITATION 审中-公开
    使用多光子荧光激发的单分子核酸序列

    公开(公告)号：US20110200989A1

    公开(公告)日：2011-08-18

    申请号：US13009442

    申请日：2011-01-19

    申请人： Gordon A. Janaway ,  Christina E. Inman ,  Joseph Beechem

    发明人： Gordon A. Janaway ,  Christina E. Inman ,  Joseph Beechem

    IPC分类号： C12Q1/68 ,  C12M1/34 ,  G01N21/75 ,  G01N21/63 ,  G01N21/64

    CPC分类号： G01N21/6452 ,  C12Q1/6818 ,  C12Q1/6869 ,  G01N21/6428 ,  G01N2021/6441 ,  Y10T436/143333 ,  C12Q2563/131 ,  C12Q2523/313

    摘要： A system for detection of nucleic acids can include an excitation source configured to transmit excitation energy to a reaction site including a single molecule of nucleic acid reacted with a two-photon absorption moiety. The system also can include an optical system configured to focus the excitation energy transmitted from the excitation source to a focal region containing the reaction site, wherein said excitation energy within the focal region is sufficient to cause two-photon absorption by the two-photon absorption moiety. The system can further include a detector configured to detect emissions generated at the reaction site resulting from two-photon absorption of the excitation energy by the two-photon absorption moiety.

    摘要翻译： 用于检测核酸的系统可以包括被配置为将激发能量传递到包括与双光子吸收部分反应的单分子核酸的反应位点的激发源。 该系统还可以包括配置成将从激发源传输的激发能量聚焦到包含反应位点的聚焦区域的光学系统，其中聚焦区域内的所述激发能足以通过双光子吸收引起双光子吸收 部分。 该系统还可以包括检测器，其被配置为检测由双光子吸收部分的激发能量的双光子吸收引起的在反应位置产生的发射。