公开(公告)号：US5231466A

公开(公告)日：1993-07-27

申请号：US739278

申请日：1991-08-01

申请人： Steven R. Erskine ,  Hernan J. Cortes ,  Yvonne M. Walbroehl ,  Curtis D. Pfeiffer

发明人： Steven R. Erskine ,  Hernan J. Cortes ,  Yvonne M. Walbroehl ,  Curtis D. Pfeiffer

IPC分类号： G01N21/45 ,  G01N21/85

CPC分类号： G01N21/45 ,  G01N21/4788 ,  G01N21/85

摘要： There is provided an apparatus for monitoring the relative concentration of an analyte in a fluid stream, with such apparatus including a capillary tube for containing the flowing fluid, optics for directing a single beam of monochromatic light through the capillary tube to produce a diffraction pattern having a number of interference fringes. A translatable photodiode is provided to monitor a portion of one of the fringes passing through a light limiting slit, whereby a signal proportional to the concentration of the analyte in the fluid is produced based upon the varying intensity of the monitored fringe. The capillary tube is preferably provided as a fused silica member having an inner flow diameter of 100 micrometers or less.

摘要翻译： 提供了一种用于监测流体流中分析物的相对浓度的装置，其中这种装置包括用于容纳流动流体的毛细管，用于将单个单色光束引导通过毛细管的光学器件，以产生具有 一些干扰条纹。 提供可平移的光电二极管以监测通过光限制狭缝的条纹中的一个的一部分，由此基于所监视的边缘的变化的强度产生与流体中分析物的浓度成比例的信号。 毛细管优选设置为内径为100微米或更小的熔融二氧化硅构件。

公开(公告)号：US5236593A

公开(公告)日：1993-08-17

申请号：US982266

申请日：1992-11-25

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

IPC分类号： G01N30/02 ,  G01N30/16 ,  G01N30/46

CPC分类号： G01N30/465 ,  G01N2030/025 ,  G01N2030/165 ,  G01N30/462

摘要： Apparatus and method for on-line coupled liquid chromatography and gas chromatography analysis. The system includes a high-volume, liquid vaporizing chamber, or interface capillary tube which allows injection of a substantial volume of sample-carrying eluent and concentrates bands, obviating adverse effects on the resolution or peak shape of the components of interest. The vaporizing chamber is interposed between a packed or wall-coated open tubular capillary liquid chromatographic column and a capillary gas chromatographic column. The capillary liquid chromatographic column permits the use of small volumes of eluent and reduced flow rates.

摘要翻译： 在线耦合液相色谱和气相色谱分析的仪器和方法。 该系统包括大容量的液体蒸发室或接口毛细管，其允许注入大量的样品携带洗脱液和浓缩物带，消除对感兴趣的组分的分辨率或峰形的不利影响。 蒸发室介于填充或壁涂开放的管状毛细管液相色谱柱和毛细管气相色谱柱之间。 毛细管液相色谱柱允许使用少量洗脱液和降低流速。

公开(公告)号：US5679255A

公开(公告)日：1997-10-21

申请号：US902144

申请日：1992-06-22

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer

IPC分类号： G01N30/02 ,  G01N30/56 ,  G01N30/60 ,  B01D15/08

CPC分类号： G01N30/56 ,  G01N2030/027 ,  G01N2030/565 ,  G01N30/6052

摘要： Highly efficient microcolumns suitable for use in chromatography, especially size excusion chromatography, are presented along with the method for making them. The method involves first obtaining a microcolumn having a first end and a second end, said microcolumn having an inner diameter of less than 1 mm. Then a slurry is prepared containing a packing material and a liquid solvent, said slurry having a concentration of more than 10 milliliters of solvent per gram of packing material. A retaining means is placed at the second end of the microcolumn, said retaining means being effective for retaining the packing material while allowing the liquid solvent to pass through the retaining means. The slurry is then added to the first end of the microcolumn at a constant pressure, said pressure being less than about 680 atmospheres. After a length of column suitable for use in chromatography has been filled, the addition of the slurry is stopped. The column is allowed to slowly decompress by allowing the solvent to exit the second end of the column.

摘要翻译： 介绍了适用于色谱，特别是大小排放色谱的高效微柱及其制备方法。 该方法首先获得具有第一端和第二端的微柱，所述微柱的内径小于1mm。 然后制备含有包装材料和液体溶剂的浆料，所述浆料的浓度大于10毫升溶剂/克包装材料。 保持装置放置在微柱的第二端，所述保持装置有效地保持包装材料，同时允许液体溶剂通过保持装置。 然后将浆料以恒定压力加入到微柱的第一端，所述压力小于约680个大气压。 在适用于色谱法的一段色谱柱已经填充之后，停止加入浆料。 通过允许溶剂离开色谱柱的第二端，允许色谱柱缓慢减压。

公开(公告)号：US4935145A

公开(公告)日：1990-06-19

申请号：US348240

申请日：1989-05-05

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

IPC分类号： G01N30/02 ,  G01N30/16 ,  G01N30/46 ,  G01N30/88

CPC分类号： G01N30/465 ,  G01N30/88 ,  G01N2030/025 ,  G01N2030/165 ,  G01N30/462

摘要： Apparatus and method for on-line coupled liquid chromatography and gas chromatography analysis includes a retention gap which allows injection of a substantial volume of sample-carrying eluent and concentrates bands, obviating adverse effects on the resolution or peak shape of the components of interest. The retention gap is used to simultaneously receive and vaporize liquid solvent in a manner highly suitable for purposes of gas chromatographically separating solutes of interest dispersed in the liquid chromatography solvent.

摘要翻译： 用于在线耦合液相色谱和气相色谱分析的装置和方法包括保留间隙，其允许注入大量样品携带洗脱液和浓缩物带，消除对感兴趣组分的分辨率或峰形的不利影响。 保留间隙用于以非常适合气相色谱分离分散在液相色谱溶剂中的目的溶质的方式同时接收和蒸发液体溶剂。

公开(公告)号：US5240604A

公开(公告)日：1993-08-31

申请号：US982265

申请日：1992-11-25

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Steven J. Martin ,  Charles G. Smith ,  Gary L. Jewett

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Steven J. Martin ,  Charles G. Smith ,  Gary L. Jewett

IPC分类号： G01N30/02 ,  G01N30/12 ,  G01N30/46 ,  G01N30/84 ,  G01N31/12 ,  G01N33/44

CPC分类号： G01N30/463 ,  G01N30/12 ,  G01N30/84 ,  G01N31/12 ,  G01N2030/025 ,  G01N2030/125 ,  G01N2030/8405 ,  G01N30/02 ,  G01N30/462 ,  G01N33/44

摘要： An on-line multidimensional system which includes a liquid chromatograph having an on-line connection to a pyrolysis probe, which in turn has on-line connection to a gas chromatograph. Preferred applications use a size-exclusion chromatograph coupled to a pyrolysis probe coupled to a gas chromatograph to simultaneously produce composition as a function of molecular weight/size information for polymeric materials.

摘要翻译： 一种在线多维系统，其包括具有与热解探针的在线连接的液相色谱仪，其又与气相色谱仪在线连接。 优选的应用使用与耦合到气相色谱仪的热解探针耦合的尺寸排阻色谱，以同时产生作为聚合物材料的分子量/尺寸信息的函数的组成。

公开(公告)号：US5522988A

公开(公告)日：1996-06-04

申请号：US318481

申请日：1994-10-05

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Bruce E. Richter

IPC分类号： G01N30/02 ,  G01N30/16 ,  G01N30/46 ,  B01D15/08

CPC分类号： G01N30/465 ,  G01N2030/025 ,  G01N2030/165 ,  G01N30/462

摘要： Apparatus and method for on-line coupled liquid chromatography and gas chromatography analysis. The system includes a high-volume, liquid vaporizing chamber, or interface capillary tube which allows injection of a substantial volume of sample-carrying eluent and concentrates bands, obviating adverse effects on the resolution or peak shape of the components of interest. The vaporizing chamber is interposed between a packed or wall-coated open tubular capillary liquid chromatographic column and a capillary gas chromatographic column. The capillary liquid chromatographic column permits the use of small volumes of eluent and reduced flow rates.

摘要翻译： 在线耦合液相色谱和气相色谱分析的仪器和方法。 该系统包括大容量的液体蒸发室或接口毛细管，其允许注入大量的样品携带洗脱液和浓缩物带，消除对感兴趣的组分的分辨率或峰形的不利影响。 蒸发室介于填充或壁涂开放的管状毛细管液相色谱柱和毛细管气相色谱柱之间。 毛细管液相色谱柱允许使用少量洗脱液和降低流速。

公开(公告)号：US5376277A

公开(公告)日：1994-12-27

申请号：US68486

申请日：1993-05-28

申请人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Steven J. Martin ,  Charles G. Smith ,  Gary L. Jewett

发明人： Hernan J. Cortes ,  Curtis D. Pfeiffer ,  Steven J. Martin ,  Charles G. Smith ,  Gary L. Jewett

IPC分类号： G01N30/02 ,  G01N30/12 ,  G01N30/46 ,  G01N30/84 ,  G01N31/12 ,  G01N33/44 ,  B01D15/08

CPC分类号： G01N30/463 ,  G01N30/12 ,  G01N30/84 ,  G01N31/12 ,  G01N2030/025 ,  G01N2030/125 ,  G01N2030/8405 ,  G01N30/02 ,  G01N30/462 ,  G01N33/44

摘要： An on-line multidimensional system which includes a liquid chromatograph having an on-line connection to a pyrolysis probe, which in turn has on-line connection to a gas chromatograph. Preferred applications use a size-exclusion chromatograph coupled to a pyrolysis probe coupled to a gas chromatograph to simultaneously produce composition as a function of molecular weight/size information for polymeric materials.

摘要翻译： 一种在线多维系统，其包括具有与热解探针的在线连接的液相色谱仪，其又与气相色谱仪在线连接。 优选的应用使用与耦合到气相色谱仪的热解探针耦合的尺寸排阻色谱，以同时产生作为聚合物材料的分子量/尺寸信息的函数的组成。

公开(公告)号：US5139681A

公开(公告)日：1992-08-18

申请号：US594403

申请日：1990-10-09

申请人： Hernan J. Cortes ,  Robert M. Campbell ,  R. Paul Himes ,  Curtis D. Pfeiffer

发明人： Hernan J. Cortes ,  Robert M. Campbell ,  R. Paul Himes ,  Curtis D. Pfeiffer

IPC分类号： B01D15/08 ,  G01N30/02 ,  G01N30/06 ,  G01N30/12 ,  G01N30/38 ,  G01N30/46

CPC分类号： G01N30/463 ,  G01N2030/062 ,  G01N2030/126 ,  G01N30/02 ,  G01N30/38 ,  G01N30/462

摘要： An on-line multidimensional chromatographic system and method which couples a liquid chromatograph to a supercritical fluid chromatograph. The system includes an inlet column for depositing a fraction of interest eluting from the liquid chromatograph, an interface interposed between the inlet column and the supercritical fluid chromatograph for receiving and trapping an analyte from the deposited fraction on an impactor surface, and a valve arrangement for controlling fluid flow throughout the system. After the fraction of interest has been deposited in the inlet column, the liquid chromatograph solvent is removed from the inlet column, and then the deposited fraction is extracted from the inlet column in a supercritical fluid stream. The supercritical fluid stream is directed into the interface, where the analyte is trapped while the supercritical fluid is decompressed and vented. A subsequent supercritical fluid stream is then used to introduce the trapped analyte directly into the analytical column of the supercritical fluid chromatograph.

摘要翻译： 一种将液相色谱仪与超临界流体色谱仪相结合的在线多维色谱系统和方法。 该系统包括用于从液相色谱仪洗脱出一部分沉积物的入口柱，介于入口柱和超临界流体色谱仪之间的界面，用于接收和捕获来自沉积部分的分析物在冲击器表面上，以及阀装置 控制流经整个系统的流体。 感兴趣的部分已经沉积在入口塔中后，液体色谱溶剂从入口塔中除去，然后以超临界流体流从入口塔提取沉积的馏分。 将超临界流体流引入界面，在该界面中分析物被捕获，同时超临界流体被减压和排气。 然后使用随后的超临界流体流将捕获的分析物直接引入超临界流体色谱仪的分析柱中。

公开(公告)号：US4529521A

公开(公告)日：1985-07-16

申请号：US526921

申请日：1983-08-26

申请人： Hernan J. Cortes ,  James C. Davis

发明人： Hernan J. Cortes ,  James C. Davis

IPC分类号： G01N13/04 ,  G01N30/02 ,  G01N30/06 ,  G01N30/14 ,  G01N33/483 ,  B01D15/08

CPC分类号： B01D63/02 ,  B01D61/243 ,  B01D61/28 ,  G01N30/06 ,  B01D2313/90 ,  G01N2030/146 ,  G01N30/02

摘要： A method for analyzing the serum phase of a latex solution by permeating low molecular weight components present in the serum phase for a known amount of time through a size selective permeable membrane to a dialysate fluid and sequentially injecting the dialysate fluid into a liquid chromatograph for subsequent analysis.

摘要翻译： 通过将存在于血清相中的低分子量组分通过尺寸选择性渗透膜渗透到透析液中并将透析液顺次注入液相色谱仪中来分析胶乳溶液的血清相的方法， 分析。

公开(公告)号：US4629705A

公开(公告)日：1986-12-16

申请号：US615985

申请日：1984-05-31

申请人： Hernan J. Cortes ,  Timothy S. Stevens

发明人： Hernan J. Cortes ,  Timothy S. Stevens

IPC分类号： G01N30/88 ,  B01D15/08 ,  G01N30/02 ,  G01N30/74 ,  G01N30/96 ,  G01N31/08 ,  G01N21/00

CPC分类号： G01N30/96 ,  G01N30/74

摘要： A versatile and highly sensitive procedure of indirect photometric chromatography in which a variable capacity pH dependent weakly basic or weakly acidic ion exchange medium is used in the chromatographic column to effect ion separations. Adjusting eluent pH allows increased detection limits without extended analysis time, reduced column efficiency, or the need for a battery of conventional strongly basic/acidic ion exchange columns.

摘要翻译： 间接光度色谱法的多功能和高灵敏度的程序，其中在色谱柱中使用可变容量的pH依赖性弱碱性或弱酸性离子交换介质来实现离子分离。 调节洗脱液pH值可以提高检测限度，无需延长分析时间，降低色谱柱效率，或需要一批常规的强碱性/酸性离子交换色谱柱。