公开(公告)号：US20170097326A1

公开(公告)日：2017-04-06

申请号：US14872139

申请日：2015-10-01

申请人： DIONEX CORPORATION ,  THERMO HYPERSIL-KEYSTONE LLC

发明人： Xiaodong LIU ,  Jennifer M. NGUYEN ,  Ronald A. SHERANT ,  Mark L. TRACY

IPC分类号： G01N30/56

CPC分类号： G01N30/56 ,  B01D15/206 ,  G01N2030/027 ,  G01N2030/565

摘要： A method of packing a chromatography column, including: dispersing chromatographic media particles in a slurry solution to form a slurry, filling a chromatography column with the chromatographic media particles by introducing the slurry to the column and applying a pressure to pack the chromatographic media particles in the chromatography column. The slurry solution is preferably aqueous based slurry solution. The pressure is preferably applied and held substantially constant for a first period at a first pressure and is applied and held substantially constant for a second period, following the first period, at a second pressure higher than the first pressure.

公开(公告)号：US09597610B2

公开(公告)日：2017-03-21

申请号：US13672819

申请日：2012-11-09

申请人： GE HEALTHCARE BIOPROCESS R&D AB

发明人： Klaus Gebauer

IPC分类号： B01D15/20 ,  B01D15/22 ,  G01N30/56 ,  B01D15/14 ,  G01N30/60

CPC分类号： B01D15/206 ,  B01J2220/56 ,  G01N30/56 ,  G01N30/6021 ,  G01N30/603 ,  G01N30/606 ,  G01N2030/565 ,  Y10T137/0402

摘要： The invention relates to a system for packing chromatography columns with a chromatography medium and packing method for use in such columns. In particular, the invention relates to a method and system for packing chromatography columns where the column and chromatography media are pre-sterilized.

公开(公告)号：US20140224738A1

公开(公告)日：2014-08-14

申请号：US14201175

申请日：2014-03-07

申请人： GE HEALTHCARE BIO-SCIENCES AB

发明人： KLAUS GEBAUER

IPC分类号： B01D15/10 ,  B65B1/04

CPC分类号： B01D15/10 ,  B01D15/1885 ,  B01D15/206 ,  G01N30/02 ,  G01N30/467 ,  G01N30/56 ,  G01N30/6043 ,  G01N2030/565

摘要： The invention provides a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.

摘要翻译： 本发明提供了一种从干燥可溶胀颗粒开始的高效色谱柱的包装方法，以及通过该方法包装的色谱柱和分离生物分子中色谱柱的用途。 在包装方法中，足以使柱体积体积的约105-120％的液体中的溶胀体积的干燥可溶胀颗粒的量转移到柱中，将柱关闭并将液体提供给塔。

公开(公告)号：US20130306563A1

公开(公告)日：2013-11-21

申请号：US13980985

申请日：2012-01-31

申请人： Andreas Blaschyk

发明人： Andreas Blaschyk

IPC分类号： B01D15/22

CPC分类号： B01D15/22 ,  G01N30/56 ,  G01N30/6021 ,  G01N30/603 ,  G01N30/6047 ,  G01N30/6069 ,  G01N2030/565

摘要： Herein is reported the use of a chromatography column support comprising at least one plane of symmetry, one axis of symmetry, at least three legs, at least three straight connectors, whereby the connectors define a plane that is perpendicular to the axis of symmetry of the support, whereby the connectors are connected to each other at the axis of symmetry, whereby each leg is connected to a connector, whereby each leg is perpendicular to the plane defined by the connectors, whereby all legs are on the same side of the plane defined by the connectors for stabilizing the packing of a chromatography column.

摘要翻译： 本文报道了使用包括至少一个对称平面，一个对称轴，至少三个腿，至少三个直线连接器的色谱柱支架，由此连接器限定了垂直于 支撑件，由此连接器在对称轴线处彼此连接，由此每个支腿连接到连接器，由此每个支腿垂直于由连接器限定的平面，由此所有支腿位于所定义的平面的同一侧上 通过用于稳定色谱柱的包装的连接器。

公开(公告)号：US08585894B1

公开(公告)日：2013-11-19

申请号：US13683199

申请日：2012-11-21

申请人： GE Healthcare Bio-Sciences AB

发明人： Per Uselius

IPC分类号： B01D15/08

CPC分类号： B01D15/14 ,  G01N2030/565

摘要： A nozzle assembly (1) provided in a chromatography column (3), said nozzle assembly comprising a nozzle tube (21) through which chromatography media slurry is to be provided to the column (3). According to the invention said nozzle assembly (1) further comprises a pivot (39) connected in a first end (40a) to a pivot point arm (35), said pivot (39) can be pivoted into at least two different locked pivot positions and said pivot (39) being connected to the nozzle tube (21) such that a nozzle tip (25) of said nozzle tube is adjusted into at least two different locked nozzle tip positions corresponding to the at least two different locked pivot positions.

摘要翻译： 提供在色谱柱（3）中的喷嘴组件（1），所述喷嘴组件包括喷嘴管（21），色谱介质浆料将通过喷嘴管（21）提供给塔（3）。 根据本发明，所述喷嘴组件（1）还包括在第一端（40a）连接到枢转点臂（35）的枢轴（39），所述枢轴（39）可以枢转到至少两个不同的锁定枢转位置 并且所述枢轴（39）连接到所述喷嘴管（21），使得所述喷嘴管的喷嘴尖端（25）被调节成对应于所述至少两个不同的锁定枢转位置的至少两个不同的锁定喷嘴尖端位置。

公开(公告)号：US20130193052A1

公开(公告)日：2013-08-01

申请号：US13754540

申请日：2013-01-30

申请人： REPLIGEN CORPORATION

发明人： Daniel P. Witt ,  William J. Wilde ,  Adrian Lowe ,  Michael Shamashkin ,  Peter Rezac ,  Alexander Slocum ,  Travis Ward

IPC分类号： B01D15/22

CPC分类号： B01D15/22 ,  B01D15/206 ,  G01N30/56 ,  G01N30/6017 ,  G01N30/6065 ,  G01N2030/562 ,  G01N2030/565 ,  Y10T29/49826 ,  Y10T137/598

摘要： If one manufactures chromatography column tubes from plastic/thermoplastic or composite materials (such as polypropylene (PP), polyethylene (PE), polyamides, acetals, or glass-filled plastics, such as glass-fiber plastics) and secures at least one of two flow distributors within the column tube with a tight interference or press fit, the resulting chromatography columns reduce or avoid the formation of dead zones around the press fit flow distributor and have an infinitely adjustable packing medium volume, also known as the medium “bed height.”

摘要翻译： 如果从塑料/热塑性材料或复合材料（如聚丙烯（PP），聚乙烯（PE）），聚酰胺，缩醛或玻璃填充塑料（如玻璃纤维塑料）生产色谱柱管，并确保至少两个 在柱管内的流动分配器具有紧密的干涉或压配合，所得的色谱柱减少或避免在压配合流量分配器周围形成死区，并且具有无限可调的包装介质体积，也称为介质“床高度”。 “

公开(公告)号：US20130186834A1

公开(公告)日：2013-07-25

申请号：US13825829

申请日：2011-09-23

申请人： John J. Vicalvi ,  Joseph Makowiecki ,  Joseph D. Crowell ,  Parrish M. Galliher

发明人： John J. Vicalvi ,  Joseph Makowiecki ,  Joseph D. Crowell ,  Parrish M. Galliher

IPC分类号： B01D15/22

CPC分类号： B01D15/22 ,  B01F7/00141 ,  B01F7/00858 ,  B01F7/161 ,  B01F7/162 ,  B01F7/1695 ,  B01F7/22 ,  B01F13/0211 ,  B01F13/065 ,  B01F13/0827 ,  B01F15/00162 ,  B01F15/00175 ,  B01F15/0022 ,  B01F15/00227 ,  B01F15/0085 ,  B01F15/065 ,  G01N30/50 ,  G01N30/56 ,  G01N30/6017 ,  G01N30/603 ,  G01N2030/565

摘要： Methods and systems for chromatography are disclosed that employ a flexible container configured to fit within a support structure and adapted to receive a filtration or absorptive medium, such as a chromatography resin. The flexible container can include at least one inlet, at least one outlet, and a separation barrier peripherally sealed within the container to separate the container into a resin containing portion and a drainage portion. The barrier can be configured to exclude the resin material from the drainage portion during use while allowing fluids to pass therethrough. The disposable chromatography system can further include one or more agitators disposed within the flexible container and adjustably configured to be raised or lowered in the flexible container. When the agitator is in the raised position, the resin packing material can operate in a settled, packed-bed configuration. Alternatively, the agitator in the lowered position permits the chromatography resin packing material to operate in a mixed, slurry configuration.

摘要翻译： 公开了用于层析的方法和系统，其采用配置成装配在支撑结构内并适于接收过滤或吸收介质如色谱树脂的柔性容器。 柔性容器可以包括至少一个入口，至少一个出口和周边地密封在容器内的分离屏障，以将容器分离成树脂容纳部分和排出部分。 挡板可以构造成在使用期间将树脂材料从排水部分排除，同时允许流体通过。 一次性色谱系统还可以包括设置在柔性容器内的一个或多个搅拌器，并可调节地构造成在柔性容器中升高或降低。 当搅拌器处于升起位置时，树脂包装材料可以以沉降的填充床构型运行。 或者，处于降低位置的搅拌器允许色谱树脂包装材料以混合的浆料配置运行。

公开(公告)号：US08066882B2

公开(公告)日：2011-11-29

申请号：US12994174

申请日：2009-05-26

申请人： Niklas Edblad

发明人： Niklas Edblad

IPC分类号： B01D15/08

CPC分类号： B01D15/206 ,  G01N30/56 ,  G01N2030/565

摘要： A method for packing a media bed in a column (3) from a slurry being a dispersion of said media particles and a liquid, said method comprising the steps of: a) estimating a slurry concentration; b) filling the column (3) with a certain volume of the slurry from a slurry tank (13) connected to the column; c) packing a test media bed from the slurry; d) detecting where the test media bed is consolidated; e) use the information about the consolidated test media bed height for providing to the column an amount of slurry that after further packing will give a user target bed height; f) packing a media bed from the slurry.

摘要翻译： 一种从作为所述介质颗粒和液体的分散体的浆料中的柱（3）中填充介质床的方法，所述方法包括以下步骤：a）估计浆料浓度; b）用一定体积的浆料从连接到该塔的浆料罐（13）中填充塔（3）; c）从浆料中包装测试介质床; d）检测测试介质床被固结的位置; e）使用关于固结的测试介质床高度的信息来向柱提供一定量的浆料，其在进一步包装之后将给予用户目标床高; f）从浆料中包装介质床。

公开(公告)号：US07452471B2

公开(公告)日：2008-11-18

申请号：US11255682

申请日：2005-10-21

申请人： Kristian Windahl

发明人： Kristian Windahl

IPC分类号： B01D15/08

CPC分类号： G01N30/56 ,  B01D15/206 ,  G01N30/6021 ,  G01N2030/565

摘要： A column packing system comprises a control unit provided with software for monitoring the column interior pressure and/or flow through the column for use in calculating the breakpoint where a movable adapter comes into contact with a consolidated bed of bed media. The calculated breakpoint is used by the control unit to determine how much further the movable adapter has to move into the column in order to achieve a desired amount of bed compression.

摘要翻译： 柱填充系统包括控制单元，该控制单元具有用于监测柱内部压力和/或流过柱的软件，用于计算可移动适配器与固定的床介质床接触的断点。 所计算的断点由控制单元用于确定可移动适配器进一步移动到柱中以进一步达到期望的床压缩量。

公开(公告)号：US20080237146A1

公开(公告)日：2008-10-02

申请号：US11955902

申请日：2007-12-13

申请人： D. Jed Harrison ,  Richard Oleschuk ,  Loranelle Shultz-Lockyear ,  Cameron Skinner ,  Paul Li

发明人： D. Jed Harrison ,  Richard Oleschuk ,  Loranelle Shultz-Lockyear ,  Cameron Skinner ,  Paul Li

IPC分类号： B01J8/00

CPC分类号： B01L3/502707 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0877 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0487 ,  G01N1/40 ,  G01N1/405 ,  G01N30/56 ,  G01N30/6095 ,  G01N2030/285 ,  G01N2030/565 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255 ,  Y10T436/2575

摘要： An on-chip packed reactor bed design is disclosed that allows for an effective exchange of packing materials such as beads at a miniaturized level. Also disclosed is a method of treating a sample within a microfluidic analysis system, comprising: providing a main channel having a trapping zone; providing a slurry of a reagent treated packing material; inducing a flow of said packing material into said trapping zone through a flow channel connected to said trapping zone to load said trapping zone and form a packed bed of said packing material; and flowing a sample containing analytes through said packed bed, said reagent treating the sample. The present invention extends the function of microfluidic analysis systems to new applications including on-chip solid phase extraction (SPE) and on-chip capillary electrochromatography (CEC). The design can be further extended to include integrated packed bed immuno- or enzyme reactors.

摘要翻译： 公开了片上堆叠反应器床设计，其允许在小型化水平上有效地交换包装材料，例如珠粒。 还公开了一种处理微流体分析系统内的样品的方法，包括：提供具有捕获区的主通道; 提供试剂处理的包装材料的浆料; 通过连接到所述捕集区的流动通道将所述包装材料流入所述捕集区，以加载所述捕集区并形成所述包装材料的填充床; 并将含有分析物的样品流经所述填充床，所述试剂处理样品。 本发明将微流控分析系统的功能扩展到包括片上固相萃取（SPE）和片上毛细管电色谱（CEC）的新应用。 该设计可以进一步扩展到包括集成的填充床免疫或酶反应器。