公开(公告)号：US09766180B2

公开(公告)日：2017-09-19

申请号：US14895477

申请日：2014-06-02

Applicant: Stichting VU-VUmc

Inventor： Gijs Jan Lodewijk Wuite ,  Erwin Johannes Gerard Peterman ,  Iddo Heller ,  Gerrit Sitters ,  Andrea Candelli ,  Stefan Walter Hell

IPC: G01N33/00 ,  G01N35/00 ,  G01N21/00 ,  C12Q1/68 ,  G01N21/64 ,  B01L3/00 ,  G02B21/00 ,  G02B21/16 ,  G02B21/32

CPC classification number: G01N21/6428 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0877 ,  B01L2400/0454 ,  C12Q1/6816 ,  G01N21/6458 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/32 ,  C12Q2565/629

Abstract: The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).

公开(公告)号：US09952421B2

公开(公告)日：2018-04-24

申请号：US15549832

申请日：2016-02-04

Applicant: Stichting Vu

Inventor： Andreas Sebastian Biebricher ,  Andrea Candelli ,  Iddo Heller ,  Niels Laurens ,  Erwin Johannes Gerard Peterman ,  Gijs Jan Lodewijk Wuite

IPC: G02B21/32 ,  G02B27/14 ,  G02B26/08 ,  G02B27/28 ,  G02B21/16 ,  G02B21/18 ,  G02B21/36 ,  G01N21/64 ,  B01L3/00 ,  G02B27/10

CPC classification number: G02B21/32 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0654 ,  B01L2400/0454 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B26/0816 ,  G02B27/106 ,  G02B27/141 ,  G02B27/283

Abstract: The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1,OT2,OT3). In one method, trapping beams (TB1,TB2,TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1,OT2,OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.

公开(公告)号：US20180024342A1

公开(公告)日：2018-01-25

申请号：US15549832

申请日：2016-02-04

Applicant: Stichting Vu

Inventor： Andreas Sebastian Biebricher ,  Andrea Candelli ,  Iddo Heller ,  Niels Laurens ,  Erwin Johannes Gerard Peterman ,  Gijs Jan Lodewijk Wuite

IPC: G02B21/32 ,  G02B27/14 ,  G02B26/08 ,  B01L3/00 ,  G02B21/16 ,  G02B21/18 ,  G02B21/36 ,  G01N21/64 ,  G02B27/10 ,  G02B27/28

CPC classification number: G02B21/32 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0654 ,  B01L2400/0454 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B26/0816 ,  G02B27/106 ,  G02B27/141 ,  G02B27/283

Abstract: The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1, OT2, OT3). In one method, trapping beams (TB1, TB2, TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1, OT2, OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.