公开(公告)号：US20160138095A1

公开(公告)日：2016-05-19

申请号：US14898112

申请日：2014-06-11

Applicant: Stichting VU-VUMC

Inventor： Erwin Johannes Gerard Peterman ,  Gijs Jan Lodewijk Wuite ,  Gerrit Sitters ,  Douwe Kamsma

IPC: C12Q1/68 ,  G01N29/06

CPC classification number: C12Q1/6837 ,  G01N29/0681 ,  G02B21/32

Abstract: A molecular manipulation system for investigating molecules, having a sample holder constructed to hold a sample comprising a plurality of molecules attached on one side to a surface in the sample holder and on another side attached to a microbead of a plurality of microbeads. The system having; an acoustic wave generator to generate an acoustic wave exerting a force on the microbeads in the sample; and a detector device to detect a response of the plurality of microbeads in the sample on the force exerted by the acoustic wave to investigate the molecules attached to the microbeads.

Abstract translation: 一种用于调查分子的分子操纵系统，具有样品架，其被构造成将包含附着在一侧的多个分子的样品保持在样品保持器中的表面上，另一侧附着在多个微珠的微珠上。 系统有 声波发生器，用于产生在样品中的微珠上施加力的声波; 以及检测器装置，用于检测样品中的多个微珠对由声波施加的力的响应，以研究附着于微珠的分子。

公开(公告)号：US09766180B2

公开(公告)日：2017-09-19

申请号：US14895477

申请日：2014-06-02

Applicant: Stichting VU-VUmc

Inventor： Gijs Jan Lodewijk Wuite ,  Erwin Johannes Gerard Peterman ,  Iddo Heller ,  Gerrit Sitters ,  Andrea Candelli ,  Stefan Walter Hell

IPC: G01N33/00 ,  G01N35/00 ,  G01N21/00 ,  C12Q1/68 ,  G01N21/64 ,  B01L3/00 ,  G02B21/00 ,  G02B21/16 ,  G02B21/32

CPC classification number: G01N21/6428 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0877 ,  B01L2400/0454 ,  C12Q1/6816 ,  G01N21/6458 ,  G02B21/0048 ,  G02B21/0076 ,  G02B21/16 ,  G02B21/32 ,  C12Q2565/629

Abstract: The present disclosure concerns a method and system for imaging a molecular strand (MS). The method comprises providing a sample volume (SV) comprising the strand (MS); providing an excitation beam (EB) having an excitation focus (EF) in the sample volume (SV); scanning the excitation focus (EF) in the sample volume (SV) along a one dimensional scanning line (SL); trapping an end of the strand (MS) in the sample volume (SV) and extending the strand (MS) along a one-dimensional trapping line (LL) parallel to the scanning line (SL); aligning the trapping line (LL) to coincide with the scanning line (SL) to have the scanning excitation focus (EF) coincide with the strand (MS); and recording the fluorescence response (FR) as a function of a plurality of distinct scanning positions (X0) of the excitation focus (EF) along the scanning line (SL).

公开(公告)号：US09952421B2

公开(公告)日：2018-04-24

申请号：US15549832

申请日：2016-02-04

Applicant: Stichting Vu

Inventor： Andreas Sebastian Biebricher ,  Andrea Candelli ,  Iddo Heller ,  Niels Laurens ,  Erwin Johannes Gerard Peterman ,  Gijs Jan Lodewijk Wuite

IPC: G02B21/32 ,  G02B27/14 ,  G02B26/08 ,  G02B27/28 ,  G02B21/16 ,  G02B21/18 ,  G02B21/36 ,  G01N21/64 ,  B01L3/00 ,  G02B27/10

CPC classification number: G02B21/32 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0654 ,  B01L2400/0454 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B26/0816 ,  G02B27/106 ,  G02B27/141 ,  G02B27/283

Abstract: The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1,OT2,OT3). In one method, trapping beams (TB1,TB2,TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1,OT2,OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.

公开(公告)号：US20180024342A1

公开(公告)日：2018-01-25

申请号：US15549832

申请日：2016-02-04

Applicant: Stichting Vu

Inventor： Andreas Sebastian Biebricher ,  Andrea Candelli ,  Iddo Heller ,  Niels Laurens ,  Erwin Johannes Gerard Peterman ,  Gijs Jan Lodewijk Wuite

IPC: G02B21/32 ,  G02B27/14 ,  G02B26/08 ,  B01L3/00 ,  G02B21/16 ,  G02B21/18 ,  G02B21/36 ,  G01N21/64 ,  G02B27/10 ,  G02B27/28

CPC classification number: G02B21/32 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0663 ,  B01L2300/0654 ,  B01L2400/0454 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/16 ,  G02B21/18 ,  G02B21/361 ,  G02B26/0816 ,  G02B27/106 ,  G02B27/141 ,  G02B27/283

Abstract: The present disclosure relates to an apparatus (100) and method for controlling a plurality of simultaneously active optical traps (OT1, OT2, OT3). In one method, trapping beams (TB1, TB2, TB3) are provided and redirected for individually controlling a respective position (X,Y) of optical traps (OT1, OT2, OT3) formed by focusing of the redirected trapping beams in a sample volume (SV). Light (L11,L20) from the sample volume (SV) corresponding to the optical traps is received. A path of a detector beam (AB) is overlapped with one of the trapping beams (TB3), wherein the detector beam has a distinct wavelength (λA) from that of the overlapping trapping beam (TB3). In one channel, the light from the sample volume is filtered according to wavelength, and only the filtered light having the wavelength (λA) of the detector beam (AB) is measured.

公开(公告)号：US20230366801A1

公开(公告)日：2023-11-16

申请号：US18030034

申请日：2021-10-05

Applicant: Stichting VU ,  LUMICKS CA HOLDING B.V.

Inventor： Andreas Sebastian Biebricher ,  Vadim Bogatyr ,  Gijs Jan Lodewijk Wuite ,  Erwin Johannes Gerard Peterman ,  Iddo Heller ,  Rogier Martijn Reijmers

IPC: G01N15/14 ,  G06T7/60 ,  G06T7/20 ,  G06T7/70 ,  G06V20/69

CPC classification number: G01N15/1404 ,  G01N15/1434 ,  G06T7/60 ,  G06T7/20 ,  G06T7/70 ,  G06V20/698 ,  G01N2015/149 ,  G01N2015/1493 ,  G01N2015/1075

Abstract: A method comprises receiving images representing manipulating cellular bodies that includes exerting force pulses to the bodies on a wall surface; analyzing the images to determine the size of the bodies and tracking locations during and after each of force pulses, the tracking locations defining first trajectories of the bodies moving away from the wall surface and trajectories of the bodies moving towards the wall surface; determining densities of the bodies using the second trajectories and a sedimentation model of the bodies moving towards the wall surface and determining body velocities based on the first trajectories and a velocity model of the bodies moving away from the wall surface; and, determining a contrast factor for each body based on the sizes and the densities, the force applied to the bodies and the body velocities and determining a compressibility for each of the bodies based on the determined contrast factors.