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公开(公告)号：US08497115B2
公开(公告)日：2013-07-30
申请号：US13350384
申请日：2012-01-13
申请人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
发明人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
CPC分类号： C12N15/81 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含核酸构建体，其包含编码信号的第一核苷酸序列 肽，其与编码多肽的第二核苷酸序列可操作地连接，其中第一个核苷酸序列与第二个核苷酸序列是异源的，第一个核苷酸序列的3'末端紧邻第二个核苷酸序列的起始密码子的上游。 本发明还涉及分离的信号肽序列以及包含与编码多肽的核苷酸序列可操作地连接的信号肽序列的构建体，载体和真菌宿主细胞。
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公开(公告)号：US20120122191A1
公开(公告)日：2012-05-17
申请号：US13350384
申请日：2012-01-13
申请人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
发明人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
CPC分类号： C12N15/81 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含核酸构建体，其包含编码信号的第一核苷酸序列 肽，其与编码多肽的第二核苷酸序列可操作地连接，其中第一个核苷酸序列与第二个核苷酸序列是异源的，第一个核苷酸序列的3'末端紧邻第二个核苷酸序列的起始密码子的上游。 本发明还涉及分离的信号肽序列以及包含与编码多肽的核苷酸序列可操作地连接的信号肽序列的构建体，载体和真菌宿主细胞。
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公开(公告)号：US20140011262A1
公开(公告)日：2014-01-09
申请号：US13945557
申请日：2013-07-18
申请人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
发明人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
CPC分类号： C12N15/81 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含核酸构建体，其包含编码信号的第一核苷酸序列 肽，其与编码多肽的第二核苷酸序列可操作地连接，其中第一个核苷酸序列与第二个核苷酸序列是异源的，第一个核苷酸序列的3'末端紧邻第二个核苷酸序列的起始密码子的上游。 本发明还涉及分离的信号肽序列以及包含与编码多肽的核苷酸序列可操作地连接的信号肽序列的构建体，载体和真菌宿主细胞。
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公开(公告)号：US20080268526A1
公开(公告)日：2008-10-30
申请号：US12135611
申请日：2008-06-09
申请人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
发明人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
IPC分类号： C12P1/00
CPC分类号： C12N15/81 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
摘要翻译： 本发明涉及产生多肽的方法，包括：（a）在有助于产生多肽的培养基中培养真菌宿主细胞，其中真菌宿主细胞包含核酸构建体，其包含编码信号的第一核苷酸序列 肽，其与编码多肽的第二核苷酸序列可操作地连接，其中第一个核苷酸序列与第二个核苷酸序列是异源的，第一个核苷酸序列的3'末端紧邻第二个核苷酸序列的起始密码子的上游。 本发明还涉及分离的信号肽序列以及包含与编码多肽的核苷酸序列可操作地连接的信号肽序列的构建体，载体和真菌宿主细胞。
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公开(公告)号：US07393664B2
公开(公告)日：2008-07-01
申请号：US10837318
申请日：2004-04-30
申请人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
发明人： Suchindra Maiyuran , Ana Fidantsef , Howard Brody
CPC分类号： C12N15/81 , C07K2319/02 , C12N15/80 , C12P21/02
摘要： The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence and the 3′ end of the first nucleotide sequence is immediately upstream of the initiator codon of the second nucleotide sequence. The present invention also relates to the isolated signal peptide sequences and to constructs, vectors, and fungal host cells comprising the signal peptide sequences operably linked to nucleotide sequences encoding polypeptides.
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6.发明授权公开(公告)号：US08716023B2
公开(公告)日：2014-05-06
申请号：US11009890
申请日：2004-12-09
CPC分类号： C12P1/02 , C12N15/113 , C12N15/1137 , C12N2310/111 , C12N2310/14 , C12N2310/53
摘要： The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expression of the target gene. The present invention also relates to the filamentous fungal strains and to methods of producing a biological substance of interest in such filamentous fungal strains.
摘要翻译： 本发明涉及用于减少或消除丝状真菌菌株中靶基因表达的方法，其包括：（a）将丝状真菌菌株的双链可转录核酸构建体插入基因组中，所述双链可转录核酸构建体包含第一核苷酸序列，其包含 可操作地连接到靶基因的同源编码区的启动子和包含靶基因的同源编码区或其部分的第二核苷酸序列，其中第一和第二核苷酸序列彼此互补，第二核苷酸 序列相对于第一个核苷酸序列处于相反方向; 和（b）通过在有利于产生干扰RNA的条件下培养丝状真菌菌株诱导由双链可转录核酸构建体编码的干扰RNA的产生; 其中所述干扰RNA与所述靶基因的RNA转录物相互作用以减少或消除所述靶基因的表达。 本发明还涉及丝状真菌菌株以及在这种丝状真菌菌株中生产目的生物物质的方法。
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7.发明申请公开(公告)号：US20050158844A1
公开(公告)日：2005-07-21
申请号：US11009890
申请日：2004-12-09
IPC分类号： C12N1/16 , C12N9/88 , C12N15/113 , C12N15/74
CPC分类号： C12P1/02 , C12N15/113 , C12N15/1137 , C12N2310/111 , C12N2310/14 , C12N2310/53
摘要： The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expression of the target gene. The present invention also relates to the filamentous fungal strains and to methods of producing a biological substance of interest in such filamentous fungal strains.
摘要翻译： 本发明涉及用于减少或消除丝状真菌菌株中靶基因表达的方法，其包括：（a）将丝状真菌菌株的双链可转录核酸构建体插入基因组中，所述双链可转录核酸构建体包含第一核苷酸序列，其包含 可操作地连接到靶基因的同源编码区的启动子和包含靶基因的同源编码区或其部分的第二核苷酸序列，其中第一和第二核苷酸序列彼此互补，第二核苷酸 序列相对于第一个核苷酸序列处于相反方向; 和（b）通过在有利于产生干扰RNA的条件下培养丝状真菌菌株诱导由双链可转录核酸构建体编码的干扰RNA的产生; 其中所述干扰RNA与所述靶基因的RNA转录物相互作用以减少或消除所述靶基因的表达。 本发明还涉及丝状真菌菌株以及在这种丝状真菌菌株中生产目的生物物质的方法。
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8.发明申请Polypeptides having Cellulolytic Enhancing Activity and Polynucleotides Encoding Same 失效具有纤维素分解增强活性的多肽和编码相同的多核苷酸公开(公告)号：US20100299788A1
公开(公告)日：2010-11-25
申请号：US12746022
申请日：2008-12-17
申请人： Paul Harris , Suchindra Maiyuran , Kimberly Brown
发明人： Paul Harris , Suchindra Maiyuran , Kimberly Brown
CPC分类号： C12N9/2434 , C07K14/37 , C11D3/38645
摘要： The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
摘要翻译： 本发明涉及具有纤维素分解增强活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。
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公开(公告)号：US08575426B2
公开(公告)日：2013-11-05
申请号：US12746003
申请日：2008-12-17
申请人： Paul Harris , Suchindra Maiyuran , Kimberly Brown
发明人： Paul Harris , Suchindra Maiyuran , Kimberly Brown
CPC分类号： C12N9/2434 , C07K14/37 , C12P19/14
摘要： The present invention relates to isolated polypeptides having cellulolytic enhancing activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
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10.发明申请POLYPEPTIDES HAVING CELLOBIOHYDROLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 有权具有环糊精活性的多肽和编码其的多核苷酸公开(公告)号：US20120156755A1
公开(公告)日：2012-06-21
申请号：US13405988
申请日：2012-02-27
申请人： Suchindra Maiyuran , Paul Harris , Hanshu Ding , Kimberly Brown
发明人： Suchindra Maiyuran , Paul Harris , Hanshu Ding , Kimberly Brown
CPC分类号： C12N9/2437 , C11D3/38645 , C12Y302/01091
摘要： The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
摘要翻译： 本发明涉及具有纤维二糖水解酶活性的分离的多肽和编码多肽的分离的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用多肽的方法。
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