公开(公告)号：US07089161B1

公开(公告)日：2006-08-08

申请号：US10045870

申请日：2002-01-10

Applicant: Sumit Verma ,  Mark Selker

Inventor： Sumit Verma ,  Mark Selker

IPC: G06F17/10

CPC classification number: H04B10/07 ,  H04B10/0799

Abstract: The present invention describes the method and system for characterizing optical receivers based on the measured impulse response and by applying linear system theory. In a first embodiment, the impulse response of an optical receiver is obtained. Convolving the impulse response with band-limited input pulses (modeled with straight-line edge pulses), a receiver output pulse can then be determined. From this pulse, an associated eye diagram can be mathematically derived and the maximum ISI can be estimated. In a second embodiment, for a given input pulse stream with a specified amount of noise, the impulse response-generated eye diagram is used to estimate the bit error rate (BER) that the receiver would yield.

Abstract translation: 本发明描述了基于测量的脉冲响应来表征光接收机的方法和系统，并且通过应用线性系统理论。 在第一实施例中，获得光接收器的脉冲响应。 使用带限输入脉冲（用直线边缘脉冲建模）来旋转脉冲响应，然后可以确定接收器输出脉冲。 从该脉冲，相关联的眼图可以在数学上得到并且可以估计最大的ISI。 在第二实施例中，对于具有指定量的噪声的给定输入脉冲流，使用脉冲响应生成的眼图来估计接收机将产生的误比特率（BER）。

公开(公告)号：US09267100B2

公开(公告)日：2016-02-23

申请号：US13385100

申请日：2012-02-02

Applicant: Mark Selker ,  Timothy Johnston ,  Barbara Paldus

Inventor： Mark Selker ,  Timothy Johnston ,  Barbara Paldus

IPC: C12M1/00 ,  G01N21/359 ,  G01N21/552 ,  G01N21/65 ,  G01N21/85

CPC classification number: C12M27/14 ,  C12M23/00 ,  C12M29/00 ,  C12M29/06 ,  G01N21/359 ,  G01N21/552 ,  G01N21/65 ,  G01N21/8507

Abstract: A composite sensor assembly for monitoring bio-processes which is suitable for use with a polymeric bioprocess vessel or with downstream equipment, and comprises: i) a port comprising a high surface tension thermoplastic having a hollow tubular portion and a base plate portion, the base plate portion being fusibly sealable to the bioreactor vessel at a hole in the wall thereof; ii) a generally opaque polymeric monitoring sensor assembly including electrical, and/or optical measurement components. The sensor assembly fits inside the bore of the hollow tubular portion of the port, and is adhesively retained therein.

Abstract translation: 一种用于监测适用于聚合物生物过程容器或与下游设备的生物过程的复合传感器组件，包括：i）包含具有中空管状部分和底板部分的高表面张力热塑性塑料的端口， 板部分在其壁的孔处易于密封到生物反应器容器; ii）包括电和/或光学测量部件的通常不透明的聚合物监测传感器组件。 传感器组件装配在端口的中空管状部分的孔内，并且粘合地保持在其中。

公开(公告)号：US20080282026A1

公开(公告)日：2008-11-13

申请号：US12150806

申请日：2008-05-01

Applicant: Mark Selker ,  Barbara Paldus ,  Charles Kamas

Inventor： Mark Selker ,  Barbara Paldus ,  Charles Kamas

IPC: G06F12/00

CPC classification number: G11C11/22 ,  A61L2/0035 ,  A61L2/24 ,  G16B15/30

Abstract: A data management system for a biological process, comprising: a. a single-use component, b. a tag assembly, including a non-volatile memory storage component, that is associated with the single-use component, c. the memory storage component including a unique identification and a memory, and at least one data element that describes a key performance or control parameter of the single-use component d. a memory reader useable to obtain the identification from the memory storage component

Abstract translation: 一种用于生物过程的数据管理系统，包括：a。 一次性使用的组件，b。 与单次使用组件相关联的标签组件，包括非易失性存储器存储组件，c。 所述存储器存储组件包括唯一标识和存储器，以及描述所述一次性组件d的关键性能或控制参数的至少一个数据元素。 可用于从存储器存储组件获得标识的存储器读取器

公开(公告)号：US20080171383A1

公开(公告)日：2008-07-17

申请号：US11725255

申请日：2007-03-17

Applicant: Mark Selker ,  Benjamin Blizard ,  Timothy Johnston ,  Barbara Paldus

Inventor： Mark Selker ,  Benjamin Blizard ,  Timothy Johnston ,  Barbara Paldus

IPC: C12M1/34

CPC classification number: C12M23/14 ,  C12M29/00 ,  C12M41/26 ,  C12M41/32 ,  C12M41/34

Abstract: A port assembly for use with a polymeric bioreactor bag said assembly comprising: i) a hollow port member comprised at least in part of a material suitable to be fusibly affixed to the wall surface of said bioreactor bag; ii) at least one fluorophore spot positioned on said port member; iii) conduit means for conveying excitation light from an optical source to said fluorophore, said conduit means being an assembly comprising at least one of a lens, an optic fiber, a curved parabolic collimator, a shaped reflector or a wave guide; iv) conduit means for conveying fluorescent emission light from said fluorophore to a photo-detector, said conduit means being an assembly comprising at least one of a lens, an optic fiber, a curved parabolic collimator, a shaped reflector or a wave guide.

Abstract translation: 一种与聚合物生物反应器袋一起使用的端口组件，所述组件包括：i）中空端口构件，其至少部分地包括适于被柔性地固定到所述生物反应器袋的壁表面的材料; ii）位于所述端口构件上的至少一个荧光团斑点; iii）用于将来自光源的激发光传送到所述荧光团的导管装置，所述导管装置是包括透镜，光纤，弯曲抛物线准直器，成形反射器或波导中的至少一个的组件; iv）用于将来自所述荧光团的荧光发射光传送到光检测器的导管装置，所述导管装置是包括透镜，光纤，弯曲抛物线准直仪，成形反射器或波导中的至少一个的组件。

公开(公告)号：US08008065B2

公开(公告)日：2011-08-30

申请号：US11728560

申请日：2007-03-26

Applicant: Mark Selker ,  Timothy Johnston ,  Barbara Paldus

Inventor： Mark Selker ,  Timothy Johnston ,  Barbara Paldus

IPC: C12M1/34 ,  C12M1/26 ,  G01N1/10

CPC classification number: C12M23/00

Abstract: A port for use with a bioreactor vessel comprising: i) a base member comprising a hollow tubular portion and a base plate configured to be sealingly affixed to a hole in the wall of a bioreactor vessel ii) a hollow, generally tubular bushing member for containing electrical, optical, microfluidic and/or chemical monitoring components which bushing member fits inside the bore of the tubular portion of the base member, both the base member and bushing member providing access to the contents of a bioreactor by; iii) a monitoring assembly inserted into the bushing member which assembly comprises means for providing incoming optical and/or electrical signals and means for collecting and transmitting measurement signals resulting from the interaction of incoming optical and/or electrical signals with the contents of a bioreactor; and iv) a cover which maintains the position and alignment of components ii) and iii) relative to the base member.

Abstract translation: 一种用于生物反应器容器的端口，包括：i）基部构件，其包括中空管状部分和底板，所述基座构造成密封地固定到生物反应器容器的壁的孔中ii）中空的大体管状衬套构件， 电气，光学，微流体和/或化学监测部件，衬套构件配合在基部构件的管状部分的孔内，基部构件和衬套构件都提供通过生物反应器的内容物的通路; iii）监测组件，其插入到衬套构件中，该组件包括用于提供输入光学和/或电信号的装置，以及用于收集和传送由输入光学和/或电信号与生物反应器的内容物相互作用而产生的测量信号的装置; 以及iv）保持组件ii）和iii）相对于基座构件的位置和对准的盖。

公开(公告)号：US07824902B2

公开(公告)日：2010-11-02

申请号：US11725255

申请日：2007-03-17

Applicant: Mark Selker ,  Benjamin Blizard ,  Timothy Johnston ,  Barbara Paldus

Inventor： Mark Selker ,  Benjamin Blizard ,  Timothy Johnston ,  Barbara Paldus

IPC: C12M1/34

CPC classification number: C12M23/14 ,  C12M29/00 ,  C12M41/26 ,  C12M41/32 ,  C12M41/34

Abstract: A port assembly for use with a polymeric bioreactor bag includes the following components: i) a hollow port member made from a material which can be fusibly affixed to the wall surface of the bioreactor bag; ii) at least one fluorophore spot positioned on the port member; iii) a conduit for conveying excitation light from an optical source to the fluorophore, which conduit can be a lens, a curved parabolic collimator, a shaped reflector or a wave guide; and iv) a second conduit for conveying fluorescent emission light from the excited fluorophore to a photo-detector, which second conduit although different from the first, can likewise be a lens, a curved parabolic collimator, a shaped reflector or a wave guide.

Abstract translation: 与聚合物生物反应器袋一起使用的端口组件包括以下部件：i）由材料制成的中空端口部件，该材料可以柔软地固定在生物反应器袋的壁表面上; ii）位于端口构件上的至少一个荧光团斑点; iii）用于将激发光从光源传输到荧光团的导管，该导管可以是透镜，弯曲抛物线准直器，成形反射器或波导管; 以及iv）用于将来自激发的荧光团的荧光发射光传送到光检测器的第二导管，该第二导管虽然不同于第一导管，但也可以是透镜，弯曲的抛物线准直器，成形反射器或波导。

公开(公告)号：US20090104653A1

公开(公告)日：2009-04-23

申请号：US11975952

申请日：2007-10-23

Applicant: Barbara Paldus ,  Mark Selker

Inventor： Barbara Paldus ,  Mark Selker

IPC: C12Q1/06

CPC classification number: C12N1/00 ,  C12Q1/06

Abstract: This invention relates to methods for monitoring and controlling bioprocesses. Specifically, it describes using quasi-real-time analytical and numerical techniques to analyze optical loss measurements calibrated to indicate cell viability, whereby it is possible to reveal process changes and/or process events such as feeding or induction. Additionally, the present invention makes it possible to accurately estimate the onset of a decrease in cell viability and/or a suitable time for cell harvesting for a cell culture growth process. Pattern recognition methods for identifying specific process events such as batch feeding, cell infection, and product precipitation are also described.

Abstract translation: 本发明涉及用于监测和控制生物过程的方法。 具体来说，它描述了使用准实时分析和数值技术来分析校准以指示细胞活力的光学损耗测量，从而可以显示过程变化和/或过程事件，例如进食或诱导。 此外，本发明使得可以精确地估计细​​胞活力降低的发生和/或用于细胞培养生长过程的细胞收获的合适时间。 还描述了用于识别特定过程事件的模式识别方法，例如分批进料，细胞感染和产物沉淀。

公开(公告)号：US07489402B2

公开(公告)日：2009-02-10

申请号：US11724818

申请日：2007-03-16

Applicant: Mark Selker ,  Benjamin Blizard ,  Timothy Johnston

Inventor： Mark Selker ,  Benjamin Blizard ,  Timothy Johnston

IPC: G01N21/64

CPC classification number: G01N21/6428 ,  G01N2021/6441

Abstract: An apparatus and method for the stimulation and collection of fluorescent signals from a target analyte. The apparatus comprises: i) a frequency modulated optical source which emits excitation light of a wavelength which will stimulate a target fluorophore to emit a fluorescent signal when illuminated by the excitation light, ii) a first optical filter interposed between the optical source and the target fluorophore, iii) a second optical filter interposed between the fluorophore and a photo-detector and positioned to receive the emitted fluorescent signal. The beam path of at least one, preferably both, of the excitation light and the fluorescent signal is transmitted substantially through free space. A data processor will normally be used to calculate and record the phase delay between the excitation light and the fluorescent signal.

Abstract translation: 用于刺激和收集来自目标分析物的荧光信号的装置和方法。 该装置包括：i）调频光源，其发射波长的激发光，该激发光将在被激发光照射时刺激目标荧光团发出荧光信号，ii）插入在光源和靶之间的第一光学滤波器 荧光团，iii）插入在所述荧光团和光检测器之间并定位成接收所发射的荧光信号的第二光学滤光器。 激发光和荧光信号的至少一个，优选两个的光束路径基本上透过自由空间传播。 通常将使用数据处理器来计算和记录激发光和荧光信号之间的相位延迟。

公开(公告)号：US20080032389A1

公开(公告)日：2008-02-07

申请号：US11728560

申请日：2007-03-26

Applicant: Mark Selker ,  Barbara Paldus ,  Timothy Johnston

Inventor： Mark Selker ,  Barbara Paldus ,  Timothy Johnston

IPC: C12M1/00

CPC classification number: C12M23/00

Abstract: A port for use with a bioreactor vessel comprising: i) a base member comprising a hollow tubular portion and a base plate configured to be sealingly affixed to a hole in the wall of a bioreactor vessel ii) a hollow, generally tubular bushing member for containing electrical, optical, microfluidic and/or chemical monitoring components which bushing member fits inside the bore of the tubular portion of the base member, both the base member and bushing member providing access to the contents of a bioreactor by; iii) a monitoring assembly inserted into the bushing member which assembly comprises means for providing incoming optical and/or electrical signals and means for collecting and transmitting measurement signals resulting from the interaction of incoming optical and/or electrical signals with the contents of a bioreactor; and iv) a cover which maintains the position and alignment of components ii) and iii) relative to the base member.

Abstract translation: 一种用于生物反应器容器的端口，包括：i）基部构件，其包括中空管状部分和底板，所述基座构造成密封地固定到生物反应器容器的壁的孔中ii）中空的大体管状衬套构件， 电气，光学，微流体和/或化学监测部件，衬套构件配合在基部构件的管状部分的孔内，基部构件和衬套构件都提供通过生物反应器的内容物的通路; iii）监测组件，其插入到衬套构件中，该组件包括用于提供输入光学和/或电信号的装置，以及用于收集和传送由输入光学和/或电信号与生物反应器的内容物相互作用而产生的测量信号的装置; 以及iv）保持组件ii）和iii）相对于基座构件的位置和对准的盖。

公开(公告)号：US20130286380A1

公开(公告)日：2013-10-31

申请号：US13506585

申请日：2012-04-30

Applicant: Mark Selker ,  Barbara Paldus

Inventor： Mark Selker ,  Barbara Paldus

IPC: G01J3/44

CPC classification number: G01J3/44 ,  G01J3/0218 ,  G01N21/65 ,  G01N2201/08 ,  G01N2201/129 ,  G02B6/02328 ,  G02B6/02385

Abstract: A multi-analyte sensor system based on hollow core photonic bandgap fiber and Raman anti-Stokes spectroscopy. The system includes: i) an inlet to introduce an analyte sample into an analyzer chamber which analyzer includes; ii) a measurement system to derive the anti-Stokes spectral peaks and/or spectra of the sample; iii) a set of reference calibrants corresponding to the analytes of which the sample is primarily comprised; iv) a second inlet to introduce said calibrants into the analyzer chamber; v) a second measurement system to derive the anti-Stokes spectral peaks and/or spectra of the calibrants vi) an outlet through which the sample and calibrants are expelled from the analyzer chamber.

Abstract translation: 基于空心光子带隙光纤和拉曼反斯托克斯光谱的多分析物传感器系统。 该系统包括：i）将分析物样品引入分析器包含的分析器室中的入口; ii）导出样品的反斯托克斯光谱峰和/或光谱的测量系统; iii）与样品主要包含的分析物相对应的一组参考校准; iv）将所述校准物引入分析器室的第二入口; v）第二测量系统，以导出校准件的反斯托克斯光谱峰值和/或光谱; vi）出口，样品和校准物通过该出口从分析器室排出。