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    Parvovirus B19 receptor and parvovirus B19 detection
    5.
    发明授权
    Parvovirus B19 receptor and parvovirus B19 detection 失效
    细小病毒B19受体和细小病毒B19检测

    公开(公告)号：US5449608A

    公开(公告)日：1995-09-12

    申请号：US34132

    申请日：1993-03-22

    申请人： Neal S. Young ,  Kevin E. Brown

    发明人： Neal S. Young ,  Kevin E. Brown

    IPC分类号： G01N33/566 ,  A61K35/76 ,  A61K38/00 ,  A61K39/23 ,  A61K48/00 ,  A61P31/12 ,  C07H21/04 ,  C07K14/015 ,  C12N5/10 ,  C12N7/00 ,  C12N15/09 ,  C12N15/866 ,  G01N33/569 ,  C12Q1/70

    CPC分类号： C12N15/86 ,  C07K14/005 ,  G01N33/56983 ,  A61K38/00 ,  A61K48/00 ,  C12N2710/14143 ,  C12N2750/14322

    摘要： The invention provides a method of detecting the presence of a parvovirus in a sample comprising contacting the sample with a purified receptor for a parvovirus, and detecting the presence of binding of parvovirus to the receptor, the presence of binding indicating the presence of parvovirus in the sample. The present invention also provides methods of purifying and removing parvoviruses from samples. The invention further provides a composition comprising a globoside, or the parvovirus B19 binding domain of globoside, in a pharmaceutically acceptable carrier. Also provided are methods of preventing or treating parvovirus B19 infection in a human subject by preventing the binding of parvovirus B19 to P antigen and methods of gene therapy utilizing parvovirus B19 and the parvovirus B19 cellular receptor.

    摘要翻译： 本发明提供了一种检测样品中细小病毒存在的方法，包括使样品与细小病毒的纯化受体接触，并检测细胞病毒与受体的结合，存在结合，指示细小病毒存在于 样品。 本发明还提供从样品中纯化和去除细小病毒的方法。 本发明进一步提供了一种组合物，其在药学上可接受的载体中包含球苷或球状蛋白的细小病毒B19结合结构域。 还提供了通过防止细小病毒B19与P抗原的结合以及使用细小病毒B19和细小病毒B19细胞受体的基因治疗方法来预防或治疗人受试者中的细小病毒B19感染的方法。

    Detection of mutational frequency and related methods
    9.
    发明授权
    Detection of mutational frequency and related methods 有权
    检测突变频率及相关方法

    公开(公告)号：US07255993B1

    公开(公告)日：2007-08-14

    申请号：US10704283

    申请日：2003-11-06

    申请人： Neal S. Young ,  Sachiko Kajigaya

    发明人： Neal S. Young ,  Sachiko Kajigaya

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6883 ,  C12Q2600/156

    摘要： Methods are provided herein for measuring the mutational frequency of a DNA molecule in cells, for example stem cells or hematopoietic cells such as CD34+ cells or granulocytes. The method includes sequencing corresponding regions of mtDNA from a set of hematopoietic cells, or a set of clonal populations of hematopoietic cells, and comparing the sequence of the corresponding regions of mtDNA from the cells, or clonal populations of cells. The method also includes the comparison of mtDNA sequences with genomic DNA sequences. Also provided are methods for screening for an agent that has a mutagenic effect on a cell. The method includes contacting, or treating, clonal populations of cells with an agent and comparing the sequence of the mtDNA obtained from the treated clonal populations of cells, with the sequence of the corresponding region of mtDNA obtained from a control clonal populations of cells.

    摘要翻译： 本文提供了用于测量细胞中DNA分子的突变频率的方法，例如干细胞或造血细胞例如CD34 +细胞或粒细胞。 该方法包括从一组造血细胞或一组造血细胞的克隆群体中对相应的mtDNA区域进行测序，并比较来自细胞或细胞的克隆群体的mtDNA的相应区域的序列。 该方法还包括mtDNA序列与基因组DNA序列的比较。 还提供了筛选对细胞具有诱变作用的试剂的方法。 该方法包括使细胞与克隆的细胞群接触或处理，并将从经处理的细胞群体获得的mtDNA的序列与从对照克隆细胞群获得的mtDNA的相应区域的序列进行比较。