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    Method for producing glycoconjugates
    1.
    发明授权
    Method for producing glycoconjugates 失效
    糖缀合物的制备方法

    公开(公告)号：US6046040A

    公开(公告)日：2000-04-04

    申请号：US123766

    申请日：1998-07-28

    申请人： Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Shin-ichiro Nishimura ,  Kuriko Yamada

    发明人： Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Shin-ichiro Nishimura ,  Kuriko Yamada

    IPC分类号： C12P19/44 ,  C12N9/10 ,  C12N11/10 ,  C12P19/18 ,  C12P7/64 ,  C12P19/00 ,  C12P19/14 ,  C12P19/26

    CPC分类号： C12N9/1048 ,  C12N11/10 ,  C12P19/18

    摘要： A method for producing glycoconjugate, which comprises the steps of:(i) binding a sugar residue to the side chain of a water-soluble polymer via a linker having a selectively cleavable linkage to give a primer, and bringing said primer into contact with an immobilized glycosyltransferase in the presence of a sugar nucleotide, to transfer a sugar residue of said sugar nucleotide to the sugar residue of said primer,(ii) elongating a sugar chain by transfer of plural sugar residues by repeating the step (i) at least once,(iii) removing, where necessary, a by-produced nucleotide or an unreacted sugar nucleotide, and(iv) repeating the steps (i)-(iii) where necessary and releasing the glycoconjugate by selectively cleaving the cleavable linkage in the linker, from the above-mentioned primer connecting the sugar chain elongated by the transfer of plural sugar residues,and a method for producing a sphingoglycolipid.

    摘要翻译： 一种制备糖缀合物的方法，其包括以下步骤：（i）通过具有选择性切割键的接头将糖残基与水溶性聚合物的侧链结合，得到底漆，并使所述底漆与 在糖核苷酸存在下固定糖基转移酶，将所述糖核苷酸的糖残基转移到所述引物的糖残基，（ii）通过重复步骤（i）至少一次转移多个糖残基来延长糖链 ，（iii）在必要时除去副产物核苷酸或未反应的糖核苷酸，和（iv）在必要时重复步骤（i） - （iii），通过选择性地切割接头中的可切割连接键释放糖缀合物， 通过连接通过多个糖残基的转移而延伸的糖链的上述引物和用于生产鞘糖脂的方法。

    Sugar chain synthesizer
    4.
    发明授权
    Sugar chain synthesizer 失效
    糖链合成器

    公开(公告)号：US06962809B1

    公开(公告)日：2005-11-08

    申请号：US10482626

    申请日：2002-07-01

    申请人： Kisaburo Deguchi ,  Genzo Hirata ,  Junkichi Miura ,  Masahito Ito ,  Shinichiro Nishimura ,  Susumu Nishiguchi ,  Atsushi Toda ,  Hiroaki Nakagawa ,  Kuriko Yamada

    发明人： Kisaburo Deguchi ,  Genzo Hirata ,  Junkichi Miura ,  Masahito Ito ,  Shinichiro Nishimura ,  Susumu Nishiguchi ,  Atsushi Toda ,  Hiroaki Nakagawa ,  Kuriko Yamada

    IPC分类号： C12P19/14 ,  C12P19/18 ,  C12M1/34

    CPC分类号： C12P19/18 ,  C12P19/14

    摘要： A sugar chain synthesizer comprising one or more reaction columns packed with immobilized glycosyltransferase and/or glycosidase; one or more separation means, arranged downstream from the reaction columns, for separating reaction products, unreaction products and byproducts contained in the solution eluted from the reaction columns; a first pump for feeding a primer of water soluble polymer and buffer solution to the reaction columns through a first selector valve; a second pump for feeding a buffer solution and sugar nucleotide solution to any one of the reaction columns through a second selector valve; one or more circulation flow paths connecting between a flow path downstream from the separation means and a flow path upstream from each of the reaction columns; and a third selector valve, arranged between the separation means and one or more circulation flow paths, for selective connection between the separation means and a desired circulation flow path. This synthesizer ensures continuous and automatic synthesis of sugar chains even if complicated.

    摘要翻译： 一种糖链合成仪，其包含一个或多个填充有固定的糖基转移酶和/或糖苷酶的反应柱; 一个或多个分离装置，布置在反应塔的下游，用于分离从反应塔洗脱的溶液中所含的反应产物，未反应产物和副产物; 第一泵，用于通过第一选择阀向反应塔供给水溶性聚合物和缓冲溶液的底漆; 第二泵，其通过第二选择阀将缓冲溶液和糖核苷酸溶液供给到任一反应塔; 连接在分离装置下游的流动路径和每个反应塔上游的流动路径之间的一个或多个循环流动路径; 以及第三选择阀，布置在所述分离装置和一个或多个循环流动路径之间，用于分离装置和期望的循环流动路径之间的选择性连接。 这种合成器即使复杂，也能确保糖链的连续和自动合成。

    Production of cotton fiber with improved fiber characteristics
    5.
    发明授权
    Production of cotton fiber with improved fiber characteristics 失效
    生产具有纤维特性提高的棉纤维

    公开(公告)号：US06563021B1

    公开(公告)日：2003-05-13

    申请号：US09760892

    申请日：2001-01-17

    申请人： Yoshihisa Kasukabe ,  Koichi Fujisawa ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen

    发明人： Yoshihisa Kasukabe ,  Koichi Fujisawa ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen

    IPC分类号： A01H100

    CPC分类号： C12N15/8261 ,  A01N49/00 ,  C07K14/415 ,  C12N15/8242 ,  Y02A40/146

    摘要： Methods are disclosed to achieve an improvement in the characteristics and yield of cotton fibers. In one method, a cotton plant of the genus Gossypium in seed form or in growth stage is treated with a brassinosteroid. In another method, an ovule culture is prepared from a cotton plant of the genus Gossypium in a brassinosteroid-containing liquid medium. Cotton fibers with improved fiber characteristics are obtained from the cotton bolls of the treated plant or from the cultured ovules. Also disclosed are a method for inducing specific genes expression in a cotton plant to produce cotton fibers with improved fiber characteristics by treatment with a brassinosteroid, and a cotton plant produced by this method; as well as a novel gene capable of changing the degree of its expression found in the ovules of a cotton plant treated with a brassinosteroid, a gene capable of hybridizing with this novel gene, a recombinant plasmid containing this novel gene, a transformant containing this recombinant plasmid, and the like.

    摘要翻译： 公开了实现棉纤维特性和产量改进的方法。 在一种方法中，种子形式或生长阶段的棉属棉花植物用油菜素固醇处理。 在另一种方法中，在含油菜素固醇的液体培养基中从棉属棉花植物制备胚珠培养物。 具有改善的纤维特性的棉纤维由处理植物的棉铃或培养的胚珠获得。 还公开了一种在棉花植物中诱导特异性基因表达的方法，以通过用油菜素甾醇处理生产具有改善的纤维特性的棉纤维和通过该方法生产的棉花植物; 以及能够改变用油菜素类固醇处理的棉花植物的胚珠中发现的表达程度的新型基因，能够与该新基因杂交的基因，含有该新基因的重组质粒，含有该重组体的转化体 质粒等。

    Production of cotton fibers with improved fiber characteristics by treatment with brassinosteroids
    6.
    发明授权
    Production of cotton fibers with improved fiber characteristics by treatment with brassinosteroids 失效
    通过处理油菜素类固醇生产具有改善纤维特性的棉纤维

    公开(公告)号：US5880110A

    公开(公告)日：1999-03-09

    申请号：US391696

    申请日：1995-02-21

    申请人： Yoshihisa Kasukabe ,  Koichi Fujisawa ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen

    发明人： Yoshihisa Kasukabe ,  Koichi Fujisawa ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen

    IPC分类号： A01H1/00 ,  A01H3/04 ,  A01N49/00 ,  C07J73/00 ,  C07K14/415 ,  C12N1/21 ,  C12N5/04 ,  C12N5/10 ,  C12N15/00 ,  C12N15/01 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  C12R1/19 ,  A01N43/04

    CPC分类号： C12N15/8261 ,  A01N49/00 ,  C07K14/415 ,  C12N15/8242

    摘要： Methods are disclosed to achieve an improvement in the characteristics and yield of cotton fibers. In one method, a cotton plant of the genus Gossypium in seed form or in growth stage is treated with a brassinosteroid. In another method, an ovule culture is prepared from a cotton plant of the genus Gossypium in a brassinosteroid-containing liquid medium. Cotton fibers with improved fiber characteristics are obtained from the cotton bolls of the treated plant or from the cultured ovules. Also disclosed are a method for inducing specific genes expression in a cotton plant to produce cotton fibers with improved fiber characteristics by treatment with a brassinosteroid, and a cotton plant produced by this method; as well as a novel gene capable of changing the degree of its expression found in the ovules of a cotton plant treated with a brassinosteroid, a gene capable of hybridizing with this novel gene, a recombinant plasmid containing this novel gene, a transformant containing this recombinant plasmid, and the like.

    摘要翻译： 公开了实现棉纤维特性和产量改进的方法。 在一种方法中，种子形式或生长阶段的棉属棉花植物用油菜素固醇处理。 在另一种方法中，在含油菜素固醇的液体培养基中从棉属棉花植物制备胚珠培养物。 具有改善的纤维特性的棉纤维由处理植物的棉铃或培养的胚珠获得。 还公开了一种在棉花植物中诱导特异性基因表达的方法，以通过用油菜素甾醇处理生产具有改善的纤维特性的棉纤维和通过该方法生产的棉花植物; 以及能够改变用油菜素甾醇处理的棉花植物的胚珠中发现的表达程度的新型基因，能够与该新基因杂交的基因，含有该新基因的重组质粒，含有该重组体的转化体 质粒等。

    Cotton plant promoters
    7.
    发明授权
    Cotton plant promoters 失效
    棉花植物启动子

    公开(公告)号：US06259003B1

    公开(公告)日：2001-07-10

    申请号：US09009443

    申请日：1998-01-20

    申请人： Koichi Fujisawa ,  Yoshihisa Kasukabe ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen ,  Song Ping

    发明人： Koichi Fujisawa ,  Yoshihisa Kasukabe ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen ,  Song Ping

    IPC分类号： A01H500

    CPC分类号： C12N15/8233 ,  C07K14/415 ,  C12N15/8216

    摘要： Novel plant promoters isolated from cotton fiber tissues are provided. The plant promoters are located upstream cotton plant genes KC03, KC18, KC22, and Gh3, all of which are expressed in cotton fibers, and contain (a) DNA having the nucleotide sequence of SEQ ID NO:1, 6, 11, or 16. Also provided are plant expression vectors prepared by introducing the plant promoters into appropriate vectors; transformed plant cells prepared by introducing the plant expression vectors into host plant cells; transformed plants regenerated from the transformed plant cells; transformed plant seeds obtained from the transformed plants; and processes for producing transformed plants from the plant seeds.

    摘要翻译： 提供从棉花纤维组织分离的新型植物启动子。 植物启动子位于棉花植物基因KC03，KC18，KC22和Gh3之上，所有这些都在棉花纤维中表达，并含有（a）具有SEQ ID NO：1,6,11或16的核苷酸序列的DNA 还提供了通过将植物启动子引入适当载体而制备的植物表达载体; 通过将植物表达载体导入宿主植物细胞而制备的转化植物细胞; 从转化的植物细胞再生的转化植物; 从转化的植物获得的转化的植物种子; 以及从植物种子产生转化植物的方法。

    Production of cotton fibers with improved fiber characteristics
    8.
    发明授权
    Production of cotton fibers with improved fiber characteristics 失效
    生产具有改善纤维特性的棉纤维

    公开(公告)号：US06225536B1

    公开(公告)日：2001-05-01

    申请号：US08867484

    申请日：1997-06-02

    申请人： Yoshihisa Kasukabe ,  Koichi Fujisawa ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen

    发明人： Yoshihisa Kasukabe ,  Koichi Fujisawa ,  Susumu Nishiguchi ,  Yoshihiko Maekawa ,  Randy Dale Allen

    IPC分类号： A01H500

    CPC分类号： C12N15/8261 ,  A01N49/00 ,  C07K14/415 ,  C12N15/8242 ,  Y02A40/146

    摘要： Methods are disclosed to achieve an improvement in the characteristics and yield of cotton fibers. In one method, a cotton plant of the genus Gossypium in seed form or in growth stage is treated with a brassinosteroid. In another method, an ovule culture is prepared from a cotton plant of the genus Gossypium in a brassinosteroid-containing liquid medium. Cotton fibers with improved fiber characteristics are obtained from the cotton bolls of the treated plant or from the cultured ovules. Also disclosed are a method for inducing specific genes expression in a cotton plant to produce cotton fibers with improved fiber characteristics by treatment with a brassinosteroid, and a cotton plant produced by this method; as well as a novel gene capable of changing the degree of its expression found in the ovules of a cotton plant treated with a brassinosteroid, a gene capable of hybridizing with this novel gene, a recombinant plasmid containing this novel gene, a transformant containing this recombinant plasmid, and the like.

    摘要翻译： 公开了实现棉纤维特性和产量改进的方法。 在一种方法中，种子形式或生长阶段的棉属棉花植物用油菜素固醇处理。 在另一种方法中，在含油菜素固醇的液体培养基中从棉属棉花植物制备胚珠培养物。 具有改善的纤维特性的棉纤维由处理植物的棉铃或培养的胚珠获得。 还公开了一种在棉花植物中诱导特异性基因表达的方法，以通过用油菜素甾醇处理生产具有改善的纤维特性的棉纤维和通过该方法生产的棉花植物; 以及能够改变用油菜素甾醇处理的棉花植物的胚珠中发现的表达程度的新型基因，能够与该新基因杂交的基因，含有该新基因的重组质粒，含有该重组体的转化体 质粒等。