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公开(公告)号：US20210311025A1
公开(公告)日：2021-10-07
申请号：US17345968
申请日：2021-06-11
发明人： Fei Liu , Utkan Demirci , Sanjiv Sam Gambhir , Viswam S. Nair
摘要： A device (“the ExoTIC device”) for the isolation of extracellular vesicles from an extracellular vesicle-containing sample in which the sample is flowed through a membrane in a flow chamber to capture and purify the extracellular vesicles on the membrane. The extracellular vesicles may be washed and collected and utilized in any one of a number of ways including, but not limited to, identifying biomarkers of a disease, identifying the presence of a biomarker in a patient to determine whether a patient has a disease, and therapeutically treating existing diseases by re-introducing the extracellular vesicles, potentially modified, back into a body.
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2.发明申请Intravascular Magnetic Wire for Detection, Retrieval or Elimination of Disease-Associated Biomarkers and Toxins 审中-公开用于检测，检索或消除疾病相关生物标志物和毒素的血管内磁线公开(公告)号：US20160354116A1
公开(公告)日：2016-12-08
申请号：US15175959
申请日：2016-06-07
CPC分类号： A61B17/52 , A61B5/05 , A61B5/14546 , A61B5/6851 , A61B10/04 , A61N2/06
摘要： A medical device and method for detection, retrieval or elimination of disease-associated toxins and biomarkers is provided. A plurality of magnets is arranged within a flexible sheath forming a flexible wire. The magnets are magnetically attached to each other, end-to-end, and arranged with their magnetic polarities alternating in direction. The magnetization direction of each of the magnets is orthogonal to the length axis of the flexible wire. The medical device is completely self-contained and does not require a bulky external field source able to maintain strong magnetic field gradients (100-10,000 T/m) along the wire, and at a radial distance (˜1 mm) to attract magnetic particles throughout the entire vein diameter at a range of physiologic velocities (1-10 cm/sec). This technology is a major step forward for the clinical relevance of CTC analysis to personalized medicine and introduces a powerful generalizable strategy for enrichment of other rare blood biomarkers.
摘要翻译： 提供了用于检测，检索或消除疾病相关毒素和生物标志物的医疗装置和方法。 多个磁体布置在形成柔性线的柔性护套内。 磁体彼此以磁性方式相互连接，端对端并以其极性在方向上交替排列。 每个磁体的磁化方向与柔性线的长度轴正交。 医疗设备是完全独立的，不需要庞大的外部场源，能够沿导线保持强磁场梯度（100-10,000 T / m），并且在径向距离（〜1mm）处吸引磁性颗粒 在整个静脉直径范围内的生理速度（1-10厘米/秒）。 该技术是CTC分析与个性化医学临床相关性的重要一步，为其他稀有血液生物标志物的富集提供了强大的可推广的策略。
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公开(公告)号：US11073511B2
公开(公告)日：2021-07-27
申请号：US16073577
申请日：2017-02-01
发明人： Fei Liu , Utkan Demirci , Sanjiv Sam Gambhir , Viswam S. Nair
摘要： A device (“the ExoTIC device”) for the isolation of extracellular vesicles from an extracellular vesicle-containing sample in which the sample is flowed through a membrane in a flow chamber to capture and purify the extracellular vesicles on the membrane. The extracellular vesicles may be washed and collected and utilized in any one of a number of ways including, but not limited to, identifying biomarkers of a disease, identifying the presence of a biomarker in a patient to determine whether a patient has a disease, and therapeutically treating existing diseases by re-introducing the extracellular vesicles, potentially modified, back into a body.
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公开(公告)号：US20180002760A2
公开(公告)日：2018-01-04
申请号：US15133996
申请日：2016-04-20
发明人： Seung-min Park , Dawson Wong , Chin Chun Ooi , Sanjiv Sam Gambhir , Viswam S. Nair , Shan X. Wang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C12Q1/686 , C12Q2600/158 , G06F17/30132 , C12Q2521/107 , C12Q2537/143
摘要： A method for identification of circulating tumor cells (CTCs) in a blood sample uses magnetic enrichment and a nanowell assay. The CTCs are magnetically labeled with cancer cell markers conjugated to magnetic nanoparticles and then separated by passing the blood sample through a magnetic sifter. The enriched CTCs are then loaded into a microfluidic single-cell molecular assay comprising an array of 25,600 or more nanowells, each containing at most a single one of the CTCs. Using multiple fluorescent gene markers, simultaneous multiple-color multiplexed gene expression of the CTCs is performed, preferably using RT-PCR. Images of fluorescence signals from individual nanowells are analyzed to identify CTCs.
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公开(公告)号：US20150118157A1
公开(公告)日：2015-04-30
申请号：US14590538
申请日：2015-01-06
CPC分类号： A61K51/0491 , C07B59/005 , C07H19/16
摘要： Embodiments of the present disclosure provide for compounds and methods of making compounds such as those shown in FIGS. 1.1A and 1.1B having formula 2, 3, 4, 5, 11, and 12 and formula 2′, 4′, and 11′,as well as uses for the compounds for imaging, and the like.
摘要翻译： 本公开的实施方案提供了制备化合物的化合物和方法，例如图1和2所示的化合物。 具有式2,3,4,5,11和12以及式2'，4'和11'的1.1A和1.1B以及用于成像的化合物的用途等。
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公开(公告)号：US11604177B1
公开(公告)日：2023-03-14
申请号：US16506942
申请日：2019-07-09
发明人： Seung-Min Park , Daeyoun Won , Brian J. Lee , Sunil Bodapati , Alexander Lozano , Diego Escobedo , Sanjiv Sam Gambhir
IPC分类号： G01N31/22 , A47K17/00 , A61B10/00 , A61B5/1171 , A61B5/1172 , G06T7/00 , G06T7/90 , G06T7/20 , A61B5/00
摘要： Devices, systems, and methods are provided for monitoring or analyzing excreta of a subject using a toilet. In one embodiment, the device includes a housing mounted adjacent a toilet bowl, a urinalysis module for deploying urinalysis strips into the toilet bowl to collect a urine sample from a subject's urine stream delivered into the toilet bowl and a urinalysis sensor to acquire data from the urinalysis strip related to the urine sample, and a stool analysis module including a camera oriented into the toilet bowl to provide images of contents of the toilet bowl. In addition, the device may include a uroflowmetry module and/or a biometric identification module. One or more sensors are provided for identifying when a subject uses the toilet, and a processor activates one or more of the modules when the sensors indicate a subject is using the toilet.
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公开(公告)号：US10920287B2
公开(公告)日：2021-02-16
申请号：US15631124
申请日：2017-06-23
申请人： Global Life Science Solutions Operations UK Ltd , The Board of Trustees of the Leland Stanford Junior University
发明人： Srabani Bhaumik , Sanjiv Sam Gambhir , Ramasamy Paulmurugan , Shahriar Yaghoubi , Byeong-Cheol Ahn , Natesh Parashurama
IPC分类号： C12N15/85 , C12Q1/6897 , C12N15/10
摘要： An expression vector, comprising a first reporter nucleic acid sequence operably linked to a first expression control sequence comprising a promoter; and a second reporter nucleic acid sequence operably linked to a second expression control sequence that comprises a response element that is activated or inactivated as one or more of the cells differentiate or dedifferentiate. Methods and kits for imaging and monitoring stem cells comprising the expression vector are also provided.
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公开(公告)号：US20220139004A1
公开(公告)日：2022-05-05
申请号：US17517401
申请日：2021-11-02
发明人： Idan Steinberg , Sanjiv Sam Gambhir
摘要： A method for photoacoustic imaging is performed by measuring RF time samples from transducer elements, and reconstructing an estimated initial pressure image from the measured RF time samples and a pre-calculated forward model matrix. The reconstructing involves minimizing the difference between the pre-calculated forward model matrix applied to the image estimate and the measured RF time samples by implementing a superiorized modified conjugate gradient least squares algorithm to minimize the total variation norm. The model matrix factors each of the transducer elements into sub-wavelength mathematical elements.
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公开(公告)号：US20210109086A1
公开(公告)日：2021-04-15
申请号：US16885028
申请日：2020-05-27
申请人： The Scripps Research Institute , The Board of Trustees of The Leland Stanford Junior University
摘要： The present invention provides methods for diagnosing lung cancer in a subject comprising (a) generating circulating tumor cell (CTC) data from a blood sample obtained from the subject based on a direct analysis comprising immunofluorescent staining and morphological characteristics of nucleated cells in the sample, wherein CTCs are identified in context of surrounding nucleated cells based on a combination of the immunofluorescent staining and morphological characteristics; (b) obtaining clinical data for the subject; (c) combining the CTC data with the clinical data to diagnose lung cancer in the subject.
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公开(公告)号：US10167515B2
公开(公告)日：2019-01-01
申请号：US15133996
申请日：2016-04-20
发明人： Seung-min Park , Dawson Wong , Chin Chun Ooi , Sanjiv Sam Gambhir , Viswam S. Nair , Shan X. Wang
IPC分类号： C12Q1/6886 , C12Q1/686 , G06F17/30
摘要： A method for identification of circulating tumor cells (CTCs) in a blood sample uses magnetic enrichment and a nanowell assay. The CTCs are magnetically labeled with cancer cell markers conjugated to magnetic nanoparticles and then separated by passing the blood sample through a magnetic sifter. The enriched CTCs are then loaded into a microfluidic single-cell molecular assay comprising an array of 25,600 or more nanowells, each containing at most a single one of the CTCs. Using multiple fluorescent gene markers, simultaneous multiple-color multiplexed gene expression of the CTCs is performed, preferably using RT-PCR. Images of fluorescence signals from individual nanowells are analyzed to identify CTCs.
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