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公开(公告)号：US20240167018A1
公开(公告)日：2024-05-23
申请号：US18317248
申请日：2023-05-15
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , Children's Medical Center Corporation
发明人： Daniel E. Bauer , Stuart H. Orkin , Neville Espi Sanjana , Ophir Shalem , Jason Wright , Feng Zhang
IPC分类号： C12N15/10
CPC分类号： C12N15/1079 , C12N15/102 , C12N15/1075 , C12N15/1082
摘要： The application relates to a deep scanning mutagenesis library to interrogate phenotypic changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use.
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公开(公告)号：US20240102004A1
公开(公告)日：2024-03-28
申请号：US18345958
申请日：2023-06-30
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , CHILDREN'S MEDICAL CENTER CORPORATION
发明人： Daniel E. Bauer , Stuart H. Orkin , Neville Espi Sanjana , Ophir Shalem , Jason Wright , Feng Zhang
IPC分类号： C12N15/10
CPC分类号： C12N15/1079 , C12N15/102 , C12N15/1075 , C12N15/1082
摘要： The application relates to a deep scanning mutagenesis library to interrogate phenotypic changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use.
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公开(公告)号：US11685917B2
公开(公告)日：2023-06-27
申请号：US15807007
申请日：2017-11-08
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , CHILDREN'S MEDICAL CENTER CORPORATION
发明人： Daniel E. Bauer , Stuart H. Orkin , Neville Espi Sanjana , Ophir Shalem , Jason Wright , Feng Zhang
IPC分类号： C12N15/10
CPC分类号： C12N15/1079 , C12N15/102 , C12N15/1075 , C12N15/1082
摘要： The application relates to a deep scanning mutagenesis library to interrogate phenotypic changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use.
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公开(公告)号：US20180119138A1
公开(公告)日：2018-05-03
申请号：US15807007
申请日：2017-11-08
申请人： THE BROAD INSTITUTE INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , CHILDREN'S MEDICAL CENTER CORPORATION
发明人： Daniel E. Bauer , Stuart H. Orkin , Neville Espi Sanjana , Ophir Shalem , Jason Wright , Feng Zhang
IPC分类号： C12N15/10
CPC分类号： C12N15/1079 , C12N15/102 , C12N15/1075 , C12N15/1082
摘要： The application relates to a deep scanning mutagenesis library to interrogate phenotypic changes in a population of cells comprising a plurality of CRISPR-Cas system guide RNAs targeting genomic sequences within at least one continuous genomic region, wherein the guide RNAs target at least 100 genomic sequences upstream of a PAM sequence for every 1000 base pairs within the continuous genomic region and methods for their use.
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公开(公告)号：US20160115488A1
公开(公告)日：2016-04-28
申请号：US14990444
申请日：2016-01-07
发明人： Feng Zhang , David Benjamin Turitz Cox , Luciano Marraffini , David Olivier Bikard , Wenyan Jiang , Neville Espi Sanjana
CPC分类号： C12N15/746 , C12N9/22 , C12N15/102 , C12N15/1082 , C12N15/113 , C12N15/63 , C12N15/70 , C12N15/74 , C12N15/85 , C12N15/8509 , C12N15/907 , C12N2310/20 , C12N2310/3519 , C12N2310/531 , C12N2800/101
摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
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公开(公告)号：US20200080094A1
公开(公告)日：2020-03-12
申请号：US16535043
申请日：2019-08-07
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： Feng Zhang , Le Cong , David Benjamin Turitz Cox , Patrick Hsu , Shuailiang Lin , Fei Ran , Randall Jeffrey Platt , Neville Espi Sanjana
摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
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公开(公告)号：US20200032278A1
公开(公告)日：2020-01-30
申请号：US16445156
申请日：2019-06-18
申请人： Massachusetts Institute of Technology , The Broad Institute, Inc. , President and Fellows of Harvard College
发明人： Feng Zhang , Le Cong , David Benjamin Turitz Cox , Patrick Hsu , Shuailiang Lin , Fei Ran , Randall Jeffrey Platt , Neville Espi Sanjana
摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
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公开(公告)号：US08993233B2
公开(公告)日：2015-03-31
申请号：US14105017
申请日：2013-12-12
申请人： The Broad Institute Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： Feng Zhang , Le Cong , Randall Jeffrey Platt , Neville Espi Sanjana , Fei Ran
IPC分类号： C12Q1/68 , C12N15/85 , C12N15/63 , C12N15/01 , C12N15/86 , C12N9/14 , C12N9/22 , C12N9/52 , C12N15/00 , C07H21/02 , C07H21/04 , A61K38/43 , A61K38/46 , A61K38/47 , C12N15/10
CPC分类号： C12N15/63 , C12N7/00 , C12N9/22 , C12N15/01 , C12N15/102 , C12N15/1082 , C12N15/85 , C12N15/86 , C12N2320/30 , C12N2740/15043
摘要： The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors with additional functional domains. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
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公开(公告)号：US20200032277A1
公开(公告)日：2020-01-30
申请号：US16445150
申请日：2019-06-18
申请人： Massachusetts Institute of Technology , The Broad Institute, Inc. , President and Fellows of Harvard College
发明人： Feng Zhang , Le Cong , David Benjamin Turitz Cox , Patrick Hsu , Shuailiang Lin , Fei Ran , Randall Jeffrey Platt , Neville Espi Sanjana
摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
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公开(公告)号：US20180327756A1
公开(公告)日：2018-11-15
申请号：US15230161
申请日：2016-08-05
申请人： THE BROAD INSTITUTE INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Feng Zhang , Le Cong , David Benjamin Turitz Cox , Patrick Hsu , Shuailiang Lin , Fei Ran , Randall Jeffrey Platt , Neville Espi Sanjana
IPC分类号： C12N15/74 , C12N9/22 , C12N15/113 , C12N15/63 , C12N15/70 , C12N15/85 , C12N15/90 , C12N15/10
CPC分类号： C12N15/746 , C12N9/22 , C12N15/102 , C12N15/1082 , C12N15/113 , C12N15/63 , C12N15/70 , C12N15/74 , C12N15/85 , C12N15/8509 , C12N15/907 , C12N2310/20 , C12N2310/3519 , C12N2310/531 , C12N2800/101
摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
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