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公开(公告)号：US11597919B2
公开(公告)日：2023-03-07
申请号：US16592744
申请日：2019-10-03
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , University of Tokyo , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Silvana Konermann , Alexandro Trevino , Mark Brigham , Fei Ran , Patrick Hsu , Chie-yu Lin , Osamu Nureki , Hiroshi Nishimasu , Ryuichiro Ishitani , Feng Zhang
IPC分类号： C12N9/22 , A61K38/46 , A61K48/00 , C12N15/113 , C12N15/90 , C12Q1/6876 , A61P43/00
摘要： The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems.
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公开(公告)号：US10577630B2
公开(公告)日：2020-03-03
申请号：US14971356
申请日：2015-12-16
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： Feng Zhang , Le Cong , Fei Ran
IPC分类号： A01K67/00 , C12N15/90 , C12N9/22 , C12N15/63 , C12N15/10 , A01K67/027 , C12N15/86 , A61K48/00 , C12N7/00
摘要： The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.
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3.发明授权Engineering of systems, methods and optimized guide compositions for sequence manipulation 有权用于序列操纵的系统，方法和优化指导组合的工程公开(公告)号：US08906616B2
公开(公告)日：2014-12-09
申请号：US14290575
申请日：2014-05-29
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： Feng Zhang , Le Cong , Patrick Hsu , Fei Ran
CPC分类号： C12N9/22 , C12N9/16 , C12N15/01 , C12N15/1082 , C12N15/52 , C12N15/63 , C12N15/79 , C12N15/85 , C12N15/86 , C12N15/902 , C12Q1/6806 , C12Y301/00
摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR-Cas system.
摘要翻译： 本发明提供了用于操纵靶序列的序列和/或活性的系统，方法和组合物。 提供了载体和载体系统，其中一些编码CRISPR复合物的一个或多个组分，以及用于设计和使用这种载体的方法。 还提供了在真核细胞中引导CRISPR复合物形成的方法以及通过使用CRISPR-Cas系统引入精确突变来选择特异性细胞的方法。
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公开(公告)号：US10946108B2
公开(公告)日：2021-03-16
申请号：US14970967
申请日：2015-12-16
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： Feng Zhang , Le Cong , Fei Ran , Matthias Heidenreich , Lukasz Swiech
摘要： The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.
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公开(公告)号：US20180312824A1
公开(公告)日：2018-11-01
申请号：US15838720
申请日：2017-12-12
申请人： The Broad Institute Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College , University of Tokyo
发明人： Feng Zhang , Winston Yan , Osamu Nureki , Kaijie Zheng , Le Cong , Hiroshi Nishimasu , Fei Ran , Yinqing Li
CPC分类号： C12N9/22 , C07K2299/00 , C07K2319/09 , C07K2319/095 , C07K2319/20 , C07K2319/70 , C12N15/102
摘要： The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are structural information on the Cas protein of the CRISPR-Cas system, use of this information in generating modified components of the CRISPR complex, vectors and vector systems which encode one or more components or modified components of a CRISPR complex, as well as methods for the design and use of such vectors and components. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. In particular the present invention comprehends optimized functional CRISPR-Cas enzyme systems. In particular the present invention comprehends engineered new guide architectures and enzymes to be used in optimized Staphylococcus aureus CRISPR-Cas enzyme systems.
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6.发明申请ENGINEERING AND OPTIMIZATION OF SYSTEMS, METHODS AND COMPOSITIONS FOR SEQUENCE MANIPULATION WITH FUNCTIONAL DOMAINS 审中-公开系统的功能和优化，用于具有功能域的序列操作的方法和组合公开(公告)号：US20150291965A1
公开(公告)日：2015-10-15
申请号：US14738398
申请日：2015-06-12
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Feng Zhang , Le Cong , Randall Jeffrey Platt , Neville Espi Sanjana , Fei Ran
CPC分类号： C12N15/63 , C12N7/00 , C12N9/22 , C12N15/01 , C12N15/102 , C12N15/1082 , C12N15/85 , C12N15/86 , C12N2320/30 , C12N2740/15043
摘要： The invention provides for engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors with additional functional domains. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
摘要翻译： 本发明提供了用于操纵靶序列的序列和/或活性的系统，方法和组合物的工程和优化。 提供了载体和载体系统，其中一些编码CRISPR复合物的一个或多个组分，以及用于设计和使用具有附加功能结构域的载体的方法。 还提供了在原核和真核细胞中引导CRISPR复合物形成的方法，以确保增强靶标识别和避免毒性的特异性。
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7.发明申请公开(公告)号：US20140273232A1
公开(公告)日：2014-09-18
申请号：US14290575
申请日：2014-05-29
申请人： THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY , PRESIDENT AND FELLOWS OF HARVARD COLLEGE
发明人： Feng Zhang , Le Cong , Patrick Hsu , Fei Ran
IPC分类号： C12N15/85
CPC分类号： C12N9/22 , C12N9/16 , C12N15/01 , C12N15/1082 , C12N15/52 , C12N15/63 , C12N15/79 , C12N15/85 , C12N15/86 , C12N15/902 , C12Q1/6806 , C12Y301/00
摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR-Cas system.
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公开(公告)号：US20210361779A1
公开(公告)日：2021-11-25
申请号：US17201347
申请日：2021-03-15
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： Feng Zhang , Le Cong , Fei Ran , Matthias Heidenreich , Lukasz Swiech
摘要： The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are delivery systems and tissues or organ which are targeted as sites for delivery. Also provided are vectors and vector systems some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity and to edit or modify a target site in a genomic locus of interest to alter or improve the status of a disease or a condition.
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9.发明授权公开(公告)号：US10711285B2
公开(公告)日：2020-07-14
申请号：US14972523
申请日：2015-12-17
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： Feng Zhang , Patrick Hsu , Chie-yu Lin , Fei Ran
摘要： The invention provides for delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in prokaryotic and eukaryotic cells to ensure enhanced specificity for target recognition and avoidance of toxicity.
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公开(公告)号：US20200063147A1
公开(公告)日：2020-02-27
申请号：US16532442
申请日：2019-08-05
申请人： The Broad Institute, Inc. , Massachusetts Institute of Technology , President and Fellows of Harvard College
发明人： Feng Zhang , Le Cong , David Benjamin Turitz Cox , Patrick Hsu , Shuailiang Lin , Fei Ran , Randall Jeffrey Platt , Neville Espi Sanjana
摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
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