公开(公告)号：US10465226B2

公开(公告)日：2019-11-05

申请号：US15519340

申请日：2015-10-16

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： Tayyaba Hasan ,  Akilan Palanisami ,  Shazia Khan

IPC: C12Q1/14 ,  B01L3/00 ,  G01N33/569 ,  G01N33/58

Abstract: One aspect of the present disclosure relates to a device for detecting a target analyte in a liquid sample. The device can comprise a housing. The housing can include an inlet for receiving a liquid sample, an outlet for removing a volume of the liquid sample from the device, a filter associated with the outlet and being sized and dimensioned to retain a target analyte on a surface thereof, and a flow system comprising at least one channel that is in communication with the inlet and the outlet. At least a portion of the at least one channel can be located substantially adjacent the surface of the filter and be shaped and dimensioned to reduce the amount of unreacted fluorescent probe available to create the background interference during detection of the target analyte.

公开(公告)号：US11635379B2

公开(公告)日：2023-04-25

申请号：US16479152

申请日：2018-01-19

Applicant: The General Hospital Corporation

Inventor： Akilan Palanisami ,  Tayyaba Hasan

IPC: B01L3/00 ,  B01L7/00 ,  G01N21/64 ,  G01N21/76 ,  C12Q1/04

Abstract: The present disclosure features portable wide field fluorimeter systems, e.g., in the form of low-cost mobile platforms, and methods to perform fluorometric assays to detect a change in fluorescence intensity in liquid samples, e.g., caused by the presence of a target analyte, e.g., a protein, e.g., an enzyme (e.g., β-lactamase) expressed by a target pathogen in a liquid sample in a point-of-care setting. In some implementations, a portable system for detecting a change in fluorescence intensity in a liquid sample includes a microfluidic device, an optical assembly including an emission filter and one or more lenses, and an analyzer device that collects and processes a fluorescent signal for the detection of a target analyte produced by the target pathogen present in the liquid sample.

公开(公告)号：US20170233785A1

公开(公告)日：2017-08-17

申请号：US15519340

申请日：2015-10-16

Applicant: THE GENERAL HOSPITAL CORPORATION DBA MASSACHUSETTS GENERAL HOSPITAL

Inventor： Tayyaba Hasan ,  Akilan Palanisami ,  Shazia Khan

IPC: C12Q1/14 ,  B01L3/00

CPC classification number: C12Q1/14 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502746 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2400/049 ,  G01N33/56938 ,  G01N33/582

Abstract: One aspect of the present disclosure relates to a device for detecting a target analyte in a liquid sample. The device can comprise a housing. The housing can include an inlet for receiving a liquid sample, an outlet for removing a volume of the liquid sample from the device, a filter associated with the outlet and being sized and dimensioned to retain a target analyte on a surface thereof, and a flow system comprising at least one channel that is in communication with the inlet and the outlet. At least a portion of the at least one channel can be located substantially adjacent the surface of the filter and be shaped and dimensioned to reduce the amount of unreacted fluorescent probe available to create the background interference during detection of the target analyte.

公开(公告)号：US11547300B2

公开(公告)日：2023-01-10

申请号：US15114881

申请日：2015-02-02

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： Tayyaba Hasan ,  Bryan Q. Spring ,  Akilan Palanisami

IPC: A61B5/00 ,  A61K49/00 ,  A61K41/00 ,  G06T7/00 ,  G01J1/48 ,  A61B1/00 ,  A61B1/04

Abstract: Methods for evaluating micrometastases in a tissue region of a subject are described. The methods include administering to the subject a detectably effective amount of a tumor-targeted photoactivatable immunoconjugate; allowing a sufficient amount of time for the tumor-targeted photoactivatable immunoconjugate to enter micrometastases in the tissue region; illuminating the tumor-targeted photoactivatable immunoconjugate; obtaining an image of the tissue region of the subject using a fluorescent imaging device, and evaluating the micrometastases in the tissue region by conducting algorithmic analysis of the image. Methods of treating micrometastases in a tissue region of a subject are also described.

公开(公告)号：US20200041413A1

公开(公告)日：2020-02-06

申请号：US16479152

申请日：2018-01-19

Applicant: The General Hospital Corporation

Inventor： Akilan Palanisami ,  Tayyaba Hasan

IPC: G01N21/64 ,  B01L3/00 ,  B01L7/00 ,  G01N21/76 ,  G01N33/573

Abstract: The present disclosure features portable wide field fluorimeter systems, e.g., in the form of low-cost mobile platforms, and methods to perform fluorometric assays to detect a change in fluorescence intensity in liquid samples, e.g., caused by the presence of a target analyte, e.g., a protein, e.g., an enzyme (e.g., β-lactamase) expressed by a target pathogen in a liquid sample in a point-of-care setting. In some implementations, a portable system for detecting a change in fluorescence intensity in a liquid sample includes a microfluidic device, an optical assembly including an emission filter and one or more lenses, and an analyzer device that collects and processes a fluorescent signal for the detection of a target analyte produced by the target pathogen present in the liquid sample.