公开(公告)号：US11635379B2

公开(公告)日：2023-04-25

申请号：US16479152

申请日：2018-01-19

Applicant: The General Hospital Corporation

Inventor： Akilan Palanisami ,  Tayyaba Hasan

IPC: B01L3/00 ,  B01L7/00 ,  G01N21/64 ,  G01N21/76 ,  C12Q1/04

Abstract: The present disclosure features portable wide field fluorimeter systems, e.g., in the form of low-cost mobile platforms, and methods to perform fluorometric assays to detect a change in fluorescence intensity in liquid samples, e.g., caused by the presence of a target analyte, e.g., a protein, e.g., an enzyme (e.g., β-lactamase) expressed by a target pathogen in a liquid sample in a point-of-care setting. In some implementations, a portable system for detecting a change in fluorescence intensity in a liquid sample includes a microfluidic device, an optical assembly including an emission filter and one or more lenses, and an analyzer device that collects and processes a fluorescent signal for the detection of a target analyte produced by the target pathogen present in the liquid sample.

公开(公告)号：US20200154710A1

公开(公告)日：2020-05-21

申请号：US16013333

申请日：2018-06-20

Applicant: The General Hospital Corporation ,  New England Biolabs

Inventor： Tayyaba Hasan ,  Ulysses W. Sallum ,  Barton Slatko ,  Jeremy Foster

IPC: A01N43/90 ,  G01N21/64 ,  C07D501/16 ,  A01N25/00

Abstract: Photoactivatable pesticide compounds and methods for the use thereof in the elimination and detection of pests are provided.

公开(公告)号：US20180161272A1

公开(公告)日：2018-06-14

申请号：US15576583

申请日：2016-05-26

Applicant: The General Hospital Corporation

Inventor： Girgis Obaid ,  Tayyaba Hasan

IPC: A61K9/127 ,  A61K47/54 ,  A61K47/69 ,  A61K41/00 ,  A61P35/00

CPC classification number: A61K9/127 ,  A61K41/0071 ,  A61K47/544 ,  A61K47/6913 ,  A61P35/00

Abstract: Provided herein is a liposome comprising: a) a conjugate comprising a lysophospholipid and a photosensitizer; b) a first derivatized phospholipid comprising a first phospholipid and a strained cyclooctyne moiety; c) a second derivatized phospholipid comprising a second phospholipid and a polyethylene glycol polymer; and d) a cationic or anionic lipid. Also provided herein is a liposome comprising: a) a conjugate comprising a lysophospholipid and a photosensitizer; b) a first derivatized phospholipid comprising a first phospholipid and a targeting moiety; c) a second derivatized phospholipid comprising a second phospholipid and a polyethylene glycol polymer; and d) a cationic or anionic lipid. The liposomes provided herein can be used, for example, in the treatment of cancer or in the imaging of cancer tumors.

公开(公告)号：US11547300B2

公开(公告)日：2023-01-10

申请号：US15114881

申请日：2015-02-02

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： Tayyaba Hasan ,  Bryan Q. Spring ,  Akilan Palanisami

IPC: A61B5/00 ,  A61K49/00 ,  A61K41/00 ,  G06T7/00 ,  G01J1/48 ,  A61B1/00 ,  A61B1/04

Abstract: Methods for evaluating micrometastases in a tissue region of a subject are described. The methods include administering to the subject a detectably effective amount of a tumor-targeted photoactivatable immunoconjugate; allowing a sufficient amount of time for the tumor-targeted photoactivatable immunoconjugate to enter micrometastases in the tissue region; illuminating the tumor-targeted photoactivatable immunoconjugate; obtaining an image of the tissue region of the subject using a fluorescent imaging device, and evaluating the micrometastases in the tissue region by conducting algorithmic analysis of the image. Methods of treating micrometastases in a tissue region of a subject are also described.

公开(公告)号：US20200041413A1

公开(公告)日：2020-02-06

申请号：US16479152

申请日：2018-01-19

Applicant: The General Hospital Corporation

Inventor： Akilan Palanisami ,  Tayyaba Hasan

IPC: G01N21/64 ,  B01L3/00 ,  B01L7/00 ,  G01N21/76 ,  G01N33/573

Abstract: The present disclosure features portable wide field fluorimeter systems, e.g., in the form of low-cost mobile platforms, and methods to perform fluorometric assays to detect a change in fluorescence intensity in liquid samples, e.g., caused by the presence of a target analyte, e.g., a protein, e.g., an enzyme (e.g., β-lactamase) expressed by a target pathogen in a liquid sample in a point-of-care setting. In some implementations, a portable system for detecting a change in fluorescence intensity in a liquid sample includes a microfluidic device, an optical assembly including an emission filter and one or more lenses, and an analyzer device that collects and processes a fluorescent signal for the detection of a target analyte produced by the target pathogen present in the liquid sample.

公开(公告)号：US11795491B2

公开(公告)日：2023-10-24

申请号：US15819345

申请日：2017-11-21

Applicant: The General Hospital Corporation

Inventor： Tayyaba Hasan ,  Ulysses W. Sallum ,  Sarika Verma ,  Gerard Nau

IPC: C12Q1/34 ,  C07D501/52 ,  C12Q1/04 ,  G01N33/542 ,  G01N33/569

CPC classification number: C12Q1/34 ,  C07D501/52 ,  C12Q1/04 ,  G01N33/542 ,  G01N33/56911 ,  G01N2333/986

Abstract: The present invention provides photosensitizer compounds for use in detecting beta-lactamase activity. Methods and kits that utilize the photosensitizer compounds of the invention for the detection of, quantitation of, and classification or typing of microbial beta-lactamases.

公开(公告)号：US20220016436A1

公开(公告)日：2022-01-20

申请号：US17292466

申请日：2019-11-11

Applicant: THE GENERAL HOSPITAL CORPORATION ,  THE UNIVERSITY OF MASSACHUSETTS

Inventor： Tayyaba Hasan ,  Jonathan P. Celli ,  Imran Rizvi ,  Filip Cuckov ,  Hui Liu

IPC: A61N5/06 ,  A61N5/067

Abstract: A low-cost light delivery system includes a light source device and a light delivery applicator. The light source device includes a light source (e.g., a light emitting diode, a laser, or the like) mounted on a passive heat sink and coupled to an optical fiber extending out of the light source device to carry a light signal. The light source device also includes a microcontroller configured to define a required irradiance to be delivered by the light signal based on a prescribed dosimetry parameter; and one or more power sources, each configured to provide power to at least one of the high powered light emitting diode and the microcontroller. The light delivery applicator can be removably connected to the optical fiber and configured to be placed proximal to an area of the patient to deliver the required irradiance of the light signal to the area of the patient.

公开(公告)号：US20220142922A1

公开(公告)日：2022-05-12

申请号：US17393214

申请日：2021-08-03

Applicant: The General Hospital Corporation

Inventor： Girgis Obaid ,  Tayyaba Hasan

IPC: A61K9/127 ,  A61K47/69 ,  A61K47/54 ,  A61K41/00 ,  A61P35/00

Abstract: Provided herein is a liposome comprising: a) a conjugate comprising a lysophospholipid and a photosensitizer; b) a first derivatized phospholipid comprising a first phospholipid and a strained cyclooctyne moiety; c) a second derivatized phospholipid comprising a second phospholipid and a polyethylene glycol polymer; and d) a cationic or anionic lipid. Also provided herein is a liposome comprising: a) a conjugate comprising a lysophospholipid and a photosensitizer; b) a first derivatized phospholipid comprising a first phospholipid and a targeting moiety; c) a second derivatized phospholipid comprising a second phospholipid and a polyethylene glycol polymer; and d) a cationic or anionic lipid. The liposomes provided herein can be used, for example, in the treatment of cancer or in the imaging of cancer tumors.

公开(公告)号：US10426388B2

公开(公告)日：2019-10-01

申请号：US15114906

申请日：2015-02-02

Applicant: THE GENERAL HOSPITAL CORPORATION

Inventor： Tayyaba Hasan ,  Srivalleesha Mallidi

IPC: A61B5/1455 ,  A61B5/00 ,  A61B5/145 ,  A61N5/06 ,  G06T7/00 ,  G16H50/30 ,  G16H30/40 ,  G16H50/20 ,  G16H20/40 ,  A61B5/055

Abstract: A method for measuring differential blood oxygen saturation in a solid tumor is described that includes the steps of obtaining a first oxygenation image of a solid tumor before, during, or immediately after a administration of vascular therapy; obtaining a second oxygenation image of the solid tumor after a devascularization time period; and determining a differential blood oxygen saturation value by comparing the first oxygenation image and the second oxygenation image. The differential blood oxygen saturation value can be compared to a blood oxygen saturation necrosis value to provide a prognosis for tumor recurrence, or to guide of the tumor.

公开(公告)号：US20170233785A1

公开(公告)日：2017-08-17

申请号：US15519340

申请日：2015-10-16

Applicant: THE GENERAL HOSPITAL CORPORATION DBA MASSACHUSETTS GENERAL HOSPITAL

Inventor： Tayyaba Hasan ,  Akilan Palanisami ,  Shazia Khan

IPC: C12Q1/14 ,  B01L3/00

CPC classification number: C12Q1/14 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/502746 ,  B01L2300/042 ,  B01L2300/044 ,  B01L2300/0681 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/12 ,  B01L2400/049 ,  G01N33/56938 ,  G01N33/582

Abstract: One aspect of the present disclosure relates to a device for detecting a target analyte in a liquid sample. The device can comprise a housing. The housing can include an inlet for receiving a liquid sample, an outlet for removing a volume of the liquid sample from the device, a filter associated with the outlet and being sized and dimensioned to retain a target analyte on a surface thereof, and a flow system comprising at least one channel that is in communication with the inlet and the outlet. At least a portion of the at least one channel can be located substantially adjacent the surface of the filter and be shaped and dimensioned to reduce the amount of unreacted fluorescent probe available to create the background interference during detection of the target analyte.