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公开(公告)号：US10926257B2
公开(公告)日：2021-02-23
申请号：US16115434
申请日：2018-08-28
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Xiaolong Qiu , Elliot Hui , Amrith Karunaratne , Erik Werner
Abstract: A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. Experiments using animal organs show that the digestion device with hydro-mincing capabilities is superior to conventional scalpel mincing and digestion based on recovery of DNA and viable single cells. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.
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公开(公告)号：US12036550B2
公开(公告)日：2024-07-16
申请号：US17180711
申请日：2021-02-19
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Xiaolong Qiu , Elliot Hui , Amrith Karunaratne , Erik Werner
IPC: B01L3/00
CPC classification number: B01L3/50273 , B01L2200/027 , B01L2200/0647 , B01L2300/042 , B01L2300/0816
Abstract: A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.
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公开(公告)号：US20210197191A1
公开(公告)日：2021-07-01
申请号：US17180711
申请日：2021-02-19
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Xiaolong Qiu , Elliot Hui , Amrith Karunaratne , Erik Werner
IPC: B01L3/00
Abstract: A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.
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公开(公告)号：US20190070605A1
公开(公告)日：2019-03-07
申请号：US16115434
申请日：2018-08-28
Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
Inventor： Jered Haun , Xiaolong Qiu , Elliot Hui , Amrith Karunaratne , Erik Werner
Abstract: A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. Experiments using animal organs show that the digestion device with hydro-mincing capabilities is superior to conventional scalpel mincing and digestion based on recovery of DNA and viable single cells. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.
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