公开(公告)号：US20190233499A1

公开(公告)日：2019-08-01

申请号：US16335519

申请日：2017-09-21

申请人： TOSOH CORPORATION ,  SAGAMI CHEMICAL RESEARCH INSTITUTE

发明人： Kouta HATAYAMA ,  Teruhiko IDE ,  Hiroyuki ITO ,  Yosuke TERAO ,  Naoki YAMANAKA ,  Satoshi ENDO

IPC分类号： C07K14/735 ,  C12N1/20 ,  C12P21/02 ,  C07K16/06 ,  C12N15/70 ,  C07K1/22

CPC分类号： C07K14/70535 ,  C07K1/22 ,  C07K16/065 ,  C12N1/20 ,  C12N15/70 ,  C12P21/02

摘要： The problem to be addressed by the present invention is to provide improved recombinant FcγRIIb and FcγRIIa that do not require refolding and exhibit high productivity and thermal stability, and to provide a method for producing the same. Said problem is solved by improved recombinant FcγRIIb comprising at least the amino acid residues of the extracellular domain of human FcγRIIb (No. 43 to No. 215 in UniProt No. P31994), wherein, in said amino acid residues, at least one amino acid substitution has occurred at a position corresponding to No. 82, 94, 98, 104, 105, or 139 in UniProt No. P31994. Said problem is also solved by improved recombinant FcγRIIa comprising at least the amino acid residues of the extracellular domain of human FcγRIIa (No. 34 to No. 206 in UniProt No. P12318-1), wherein, in said amino acid residues, at least one amino acid substitution has occurred at a position corresponding to No. 73, 85, 89, 95, 96, or 130 in UniProt No. P12318-1.

公开(公告)号：US20210116445A1

公开(公告)日：2021-04-22

申请号：US17253824

申请日：2019-06-18

申请人： TOSOH CORPORATION

发明人： Yosuke TERAO ,  Yasuyuki AKIYAMA ,  Satoshi ENDO ,  Ryoko WATANABE

IPC分类号： G01N33/538 ,  G01N33/543

摘要： An object of the present invention is to provide a method for detecting the presence or absence of a disease, the risk of developing a disease, the degree of progression of a disease and/or the degree of progression of aging, in a subject. The present invention achieves the above described object by a method including the following steps (a) to (c): (a) adding a solution containing an antibody obtained from a subject to a column filled with an insoluble carrier on which an Fc-binding protein is immobilized, to allow the antibody to be adsorbed on the carrier; (b) eluting the antibody adsorbed on the carrier using an eluent, to obtain data of a separation pattern of the antibody; and (c) detecting the presence or absence of a disease, the risk of developing a disease, the degree of progression of a disease and/or the degree of progression of aging, in the subject, using the data as an indicator.

公开(公告)号：US20210002367A1

公开(公告)日：2021-01-07

申请号：US16971431

申请日：2019-02-21

申请人： TOSOH CORPORATION

发明人： Masumi TAKAYAMA ,  Yosuke TERAO

IPC分类号： C07K16/28 ,  C12N15/64 ,  C07K1/14

摘要： An Fc-binding protein having at least amino acid residues of the extracellular domain of the amino acid sequence of wildtype human FcγRIIa (UniProt Accession Number: P12318) wherein the amino acid residues in the domain have an amino acid substitution or deletion at at least a specific position, a method for producing the protein, and an antibody adsorbent using the protein.

公开(公告)号：US20170218044A1

公开(公告)日：2017-08-03

申请号：US15321916

申请日：2015-06-24

申请人： TOSOH CORPORATION

发明人： Yoshiharu ASAOKA ,  Toru TANAKA ,  Yosuke TERAO ,  Naoki YAMANAKA ,  Natsuko KIZU ,  Masaru AOKI ,  Teruhiko IDE

IPC分类号： C07K14/735 ,  B01D15/38 ,  B01J20/281 ,  G01N33/68 ,  C07K1/22 ,  C07K16/00

摘要： Provided are: an Fc-binding protein having improved stability, particularly to heat and acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent. Specifically provided are: an Fc-binding protein having improved stability to heat and acid, achieved by substituting an amino-acid residue in a specific position in the extracellular region of human FcyRIIIa with another specific amino acid; a method for producing the protein; an antibody adsorbent using the protein; and a method for separating the antibodies using the adsorbent.

公开(公告)号：US20210277053A1

公开(公告)日：2021-09-09

申请号：US16762306

申请日：2018-11-08

申请人： TOSOH CORPORATION

发明人： Naoki YAMANAKA ,  Yukie UCHIDA ,  Yosuke TERAO

IPC分类号： C07K1/14 ,  C07K14/31

摘要： The invention aims to provide an immunoglobulin-binding protein having improved chemical stability, especially stability against alkali. The object can be achieved by improving stability against alkali by substituting an amino acid residue(s) at a particular position(s) in an immunoglobulin-binding domain such as domain C of protein A derived from a bacterium belonging to the genus Staphylococcus, to another/other particular amino acid residue(s).

公开(公告)号：US20210261605A1

公开(公告)日：2021-08-26

申请号：US16759039

申请日：2018-10-26

申请人： TOSOH CORPORATION

发明人： Ryoko WATANABE ,  Satoshi ENDO ,  Yosuke TERAO ,  Seigo OE

IPC分类号： C07K1/22 ,  C12P21/02

摘要： The present invention relates to an Fc-binding protein exhibiting improved alkaline resistance, a method for producing said protein, an antibody adsorbent obtained by immobilizing said protein on a carrier, and a method for separating an antibody using said adsorbent.

公开(公告)号：US20200283779A1

公开(公告)日：2020-09-10

申请号：US16606486

申请日：2018-04-13

申请人： TOSOH CORPORATION

发明人： Yosuke TERAO ,  Naoki YAMANAKA ,  Natsuko KIZU ,  Satoshi ENDO ,  Seigo OE

IPC分类号： C12N15/70 ,  C07K14/435 ,  C07K1/14

摘要： The present invention addresses the problem of providing FcRn having improved stability with respect to heat and acids, a method for producing said FcRn, an antibody adsorbent in which said FcRn is used, and an antibody isolation method in which said adsorbent is used. The above problem is solved by substituting an amino acid residue at a specific position in an extracellular region of a human FcRn α chain and/or a β2 microglobulin region of a human FcRn β chain by another specific amino acid.

公开(公告)号：US20200223885A1

公开(公告)日：2020-07-16

申请号：US16486380

申请日：2018-02-07

申请人： TOSOH CORPORATION

发明人： Yosuke TERAO ,  Yoshiharu ASAOKA ,  Ryoko OTAKE ,  Satoshi ENDO ,  Naoki YAMANAKA ,  Yukie YAMAMOTO ,  Seigo OE

IPC分类号： C07K1/22 ,  C07K16/00 ,  C07K14/00

摘要： The present application addresses the problem of providing an Fc-binding protein having an improved antibody separation ability. The present application also addresses the problem of providing a high-accuracy antibody separation method using an insoluble carrier having the protein immobilized thereon. The problems can be solved by: an Fc-binding protein in which at least an amino acid substitution at a specific position therein occurs and which has reduced affinity for an antibody; and an antibody separation method including allowing an equilibration buffer solution to pass through a column in which an insoluble carrier having the protein immobilized thereon is filled to equilibrate the column, adding a solution containing an antibody to cause the adsorption of the antibody onto the carrier, and eluting the antibody adsorbed on the carrier using an elution solution.