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    Biocatalyst for production of D-lactic acid
    4.
    发明授权
    Biocatalyst for production of D-lactic acid 有权
    用于生产D-乳酸的生物催化剂

    公开(公告)号：US08669093B2

    公开(公告)日：2014-03-11

    申请号：US10573813

    申请日：2004-09-17

    申请人： Mitsufumi Wade ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

    发明人： Mitsufumi Wade ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

    IPC分类号： C12N1/21 ,  C12P7/56 ,  C12N1/26

    CPC分类号： C12P7/56

    摘要： A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.

    摘要翻译： 提供高产率D-乳酸的制造方法，提供高纯度的高纯度D-副乳酸和副产物有机酸的生产方法。 在一个方面，培养其中丙酮酸甲酸裂合酶（pfl）的活性失活或降低，并且进一步增强大肠杆菌来源的NADH依赖性D-乳酸脱氢酶（ldhA）的活性的微生物，以有效地产生D- 乳酸。 关于增强ldhA活性的方法，通过在基因组上连接编码ldhA的基因与控制糖酵解途径参与蛋白质的表达的基因的启动子，核酸生物合成途径或氨基酸生物合成途径 与使用表达载体增强基因表达的方法相比，获得了合适的结果。 培养其中dld基因基本上失活或减少的微生物被培养以产生具有降低的丙酮酸浓度的高质量D-乳酸。

    Biocatalyst for production of d-lactic acid (as amended)
    5.
    发明申请
    Biocatalyst for production of d-lactic acid (as amended) 有权
    用于生产d-乳酸的生物催化剂（经修订）

    公开(公告)号：US20070065930A1

    公开(公告)日：2007-03-22

    申请号：US10573813

    申请日：2004-09-17

    申请人： Mitsufumi Wada ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

    发明人： Mitsufumi Wada ,  Toshihiro Oikawa ,  Daisuke Mochizuki ,  Junko Tokuda ,  Miyuki Kawashima ,  Tadashi Araki ,  Reiko Abe ,  Hitoki Miyake ,  Hitoshi Takahashi ,  Hideki Sawai ,  Takashi Mimitsuka ,  Takashi Morishige ,  Yosuke Higashi

    IPC分类号： C12P7/56 ,  C12N1/21

    CPC分类号： C12P7/56

    摘要： A method for producing D-lactic acid in high yield, and to provide a method for producing D-lactic acid with high selectivity, in which optical purity is high and a by-product organic acid is small. In one aspect, a microorganism, wherein activity of pyruvate formate-lyase (pfl) is inactivated or decreased, and further activity of Escherichia coli-derived NADH-dependent D-lactate dehydrogenase (ldhA) is enhanced, is cultured to efficiently produce D-lactic acid. With regard to a method for enhancing ldhA activity, by linking, on a genome, a gene encoding ldhA with a promoter of a gene which controls expression of a protein involved in a glycolytic pathway, a nucleic acid biosynthesis pathway or an amino acid biosynthesis pathway, suitable results are obtained compared to the method for enhancing expression of the gene using an expression vector. A microorganism in which a dld gene is substantially inactivated or decreased is cultured to produce high quality D-lactic acid with reduced concentration of pyruvic acid.

    摘要翻译： 提供高产率D-乳酸的制造方法，提供高纯度的高纯度D-副乳酸和副产物有机酸的生产方法。 在一个方面，培养其中丙酮酸甲酸裂合酶（pfl）的活性失活或降低，并且进一步增强大肠杆菌来源的NADH依赖性D-乳酸脱氢酶（ldhA）的活性的微生物，以有效地产生D- 乳酸。 关于增强ldhA活性的方法，通过在基因组上连接编码ldhA的基因与控制糖酵解途径参与蛋白质的表达的基因的启动子，核酸生物合成途径或氨基酸生物合成途径 与使用表达载体增强基因表达的方法相比，获得了合适的结果。 培养其中dld基因基本上失活或减少的微生物被培养以产生具有降低的丙酮酸浓度的高质量D-乳酸。

    PRESSURIZED-FLUID CONTROL MECHANISM AND PRESSURIZED-FLUID SUPPLY DEVICE
    6.
    发明申请
    PRESSURIZED-FLUID CONTROL MECHANISM AND PRESSURIZED-FLUID SUPPLY DEVICE 审中-公开
    加压流体控制机构和加压流体供应装置

    公开(公告)号：US20100243681A1

    公开(公告)日：2010-09-30

    申请号：US12679958

    申请日：2008-09-25

    申请人： Ryouhei Abe ,  Reiko Abe

    发明人： Ryouhei Abe ,  Reiko Abe

    IPC分类号： B65D83/00

    CPC分类号： F17C13/04 ,  F16K1/303 ,  F17C2201/0109 ,  F17C2201/0119 ,  F17C2201/058 ,  F17C2203/0617 ,  F17C2203/0636 ,  F17C2205/0111 ,  F17C2205/0308 ,  F17C2205/032 ,  F17C2205/0329 ,  F17C2205/0382 ,  F17C2205/0394 ,  F17C2209/2109 ,  F17C2221/013 ,  F17C2223/0153 ,  F17C2223/035 ,  F17C2260/036 ,  F17C2270/0518 ,  F17C2270/0718 ,  F17C2270/0745

    摘要： A pressurized-fluid control mechanism for controlling the ejection of a compressed carbonic gas C charged into a gas container vessel (3), and a Pressurized-Fluid Supply device that makes use of the pressurized-fluid control mechanism, are disclosed. The pressurized-fluid control mechanism is mounted on the gas container vessel filled with the compressed carbonic gas C to control the ejection of the compressed carbonic gas C charged into the gas container vessel. The pressurized-fluid control mechanism includes a sealing plate (13) that stops up an opening (4) of the gas container vessel, and an acute pointed member (17) provided towards its extreme end. A sealing part (19) progressively reduced in its diameter is formed at an extreme end of the acute pointed member. The pressurized-fluid control mechanism also includes a coil spring (23) that biases the acute pointed member. The acute pointed member (17), used in this pressurized-fluid control mechanism, is arranged within the gas container vessel and adapted to be moved back and forth in the gas container vessel to open/close an ejection opening (18) formed in the sealing plate. The acute pointed member is biased by the coil spring to fit a sealing part into the ejection opening to close the opening.

    摘要翻译： 公开了一种用于控制装入气体容器（3）中的压缩碳气C的喷射的加压流体控制机构，以及利用加压流体控制机构的加压流体供给装置。 加压流体控制机构安装在填充有压缩碳气C的气体容器容器中，以控制装入气体容器的压缩碳气C的喷射。 压力流体控制机构包括阻挡气体容器容器的开口（4）的密封板（13）和朝向其最末端设置的锐角构件（17）。 形成在尖锐部件的最末端的直径逐渐减小的密封部件（19）。 加压流体控制机构还包括偏压急尖部件的螺旋弹簧（23）。 在该加压流体控制机构中使用的急尖尖构件（17）设置在气体容器内，适于在气体容器容器中前后移动，以打开/关闭形成在该压力流体控制机构中的喷射口（18） 密封板。 尖锐的构件由螺旋弹簧偏压以将密封部件装配到喷射口中以封闭开口。