公开(公告)号：US20110057145A1

公开(公告)日：2011-03-10

申请号：US12876562

申请日：2010-09-07

申请人： Tadashi Matsunaga ,  Tsuyoshi Tanaka ,  Keiichi Hatakeyama ,  Takeo Tanaami ,  Hitoshi Wake ,  Tomoyuki Taguchi ,  Takeyuki Mogi

发明人： Tadashi Matsunaga ,  Tsuyoshi Tanaka ,  Keiichi Hatakeyama ,  Takeo Tanaami ,  Hitoshi Wake ,  Tomoyuki Taguchi ,  Takeyuki Mogi

IPC分类号： H01F1/00

CPC分类号： H01F1/42

摘要： There is provided a method for preparing dendrimer-modified magnetic fine particles wherein such particles can be made within a shorter time and more inexpensively than in the above-stated prior art processes and lot-to-lot variations in properties are lessened. The method for preparing dendrimer-fixed magnetic fine particles comprises the steps of providing magnetic particles having a functional group at a surface thereof, providing a dendrimer having a functional group at a base end portion thereof and synthesized to a desired generation and binding the functional group of the magnetic particles and the functional group of the dendrimer directly or indirectly through a crosslinking agent.

摘要翻译： 提供了一种制备树枝状聚合物改性的磁性细颗粒的方法，其中这种颗粒可以在比上述现有技术方法更短的时间和更廉价的情况下制备，并且批次间的性质变化减少。 制备树枝状大分子固定磁性微粒的方法包括以下步骤：在其表面提供具有官能团的磁性颗粒，提供在其基端部分具有官能团的树枝状聚合物，并合成所需的一代并结合官能团 的磁性颗粒和树枝状聚合物的官能团直接或间接地通过交联剂。

公开(公告)号：US08877918B2

公开(公告)日：2014-11-04

申请号：US13362373

申请日：2012-01-31

申请人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

发明人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

IPC分类号： C07H21/00 ,  C12N15/10 ,  C12N1/06 ,  C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N1/06 ,  C12N15/1003 ,  C12Q2527/101 ,  C12Q2527/113

摘要： There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state.

摘要翻译： 提供了适用于较宽范围的微生物并且能够快速提取核酸的核酸提取方法。 核酸提取方法包括以下步骤：将细胞悬浮液引入容器，密封容器，并将加热器预热至不低于100℃的设定温度。此外，该方法包括使容器接触的步骤 加热器加热至设定温度，从而将容纳在容器中的细胞悬浮液加热至规定的最高温度至不低于100℃，容器保持密封状态。

公开(公告)号：US20120197008A1

公开(公告)日：2012-08-02

申请号：US13362373

申请日：2012-01-31

申请人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

发明人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

IPC分类号： C07H1/08

CPC分类号： C12Q1/6806 ,  C12N1/06 ,  C12N15/1003 ,  C12Q2527/101 ,  C12Q2527/113

摘要： There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state.

摘要翻译： 提供了适用于较宽范围的微生物并且能够快速提取核酸的核酸提取方法。 核酸提取方法包括以下步骤：将细胞悬浮液引入容器中，密封容器，并将加热器预热至不低于100℃的设定温度。此外，该方法包括使容器接触的步骤 加热器加热到设定温度，由此将容器保持在密封状态下，将容纳在容器中的细胞悬浮液加热至规定的最高温度至不低于100℃。

公开(公告)号：US5217892A

公开(公告)日：1993-06-08

申请号：US908894

申请日：1992-07-08

申请人： Tadashi Matsunaga ,  Hitoshi Wake ,  Mayumi Ono ,  Kiyoshi Hishinuma ,  Hironori Umetsu

发明人： Tadashi Matsunaga ,  Hitoshi Wake ,  Mayumi Ono ,  Kiyoshi Hishinuma ,  Hironori Umetsu

IPC分类号： A01H4/00 ,  C12N5/00 ,  C12N5/04

CPC分类号： A01H4/006 ,  C12N5/0025 ,  C12N5/04

摘要： A method of plant tissue culture which comprises culturing a tissue or an organ of a plant, a part of the same or cultured cells in a medium containing a culture filtrate and/or an extract of a photosynthetic procaryotic microorganism.Such a culturing method effectively proliferates the plant tissues and cultured cells, and also promotes the formation of adventive embryos, regeneration of the plant body and production of useful substances formed by that plant.Strains of cyanobacteria or photosynthetic bacteria are preferably used as the photosynthetic procaryotic microorganism.

摘要翻译： PCT No.PCT / JP89 / 00741 Sec。 371 1990年3月21日 102（e）1990年3月21日PCT PCT日期：1989年7月25日PCT公布。 出版物WO90 / 00855 1990年2月8日。一种植物组织培养方法，其包括在含有培养滤液和/或光合原核生物微生物提取物的培养基中培养植物的组织或器官，其一部分或培养细胞 。 这种培养方法有效地增殖植物组织和培养细胞，并且还促进形成胚胎，植物体的再生和由该植物形成的有用物质的生产。 优选使用蓝细菌或光合细菌菌株作为光合原核生物。

公开(公告)号：US06197168B1

公开(公告)日：2001-03-06

申请号：US09426658

申请日：1999-10-25

申请人： Tadashi Matsunaga ,  Tsuruo Nakayama ,  Hitoshi Wake ,  Kin-ichi Ozawa ,  Noriyuki Nakamura ,  Nobuyuki Murakami ,  Hiromichi Takahashi ,  Toshihiro Takimoto ,  Hideo Kadoi

发明人： Tadashi Matsunaga ,  Tsuruo Nakayama ,  Hitoshi Wake ,  Kin-ichi Ozawa ,  Noriyuki Nakamura ,  Nobuyuki Murakami ,  Hiromichi Takahashi ,  Toshihiro Takimoto ,  Hideo Kadoi

IPC分类号： C23F1300

CPC分类号： B63B59/04 ,  C02F1/48 ,  C02F2303/04 ,  C23F13/00

摘要： An electrochemical stain prevention apparatus of a submerged structure comprising a submerged structure of which at least the stain prevention surface is formed of a conductive film that does not generate chlorine even by applying a potential of 5 V vs. SCE or less, a counter electrode located so as not to contact with the submerged structure, and a power supply unit for passing a direct current through the submerged structure having the conductive film formed thereon and the counter electrode. Aquatic organisms adhered to the surface of the conductive film can effectively be controlled by applying a potential of from 0.1 to 5 V vs. SCE to the submerged structure of such a stain prevention apparatus without generating chlorine. A potential applied to the conductive film of the submerged structure can be controlled with good accuracy by disposing a reference electrode between the submerged structure and the counter electrode. As the conductive film formed on the substrate of the submerged structure, a sprayed coating film made of a metal nitride can preferably be used.

摘要翻译： 一种浸没结构的电化学防污染装置，包括浸没结构，其至少防污染表面由不产生氯的导电膜形成，即使通过施加5V与SCE以下的电位，也可以使用对置电极 以便不与浸没结构接触;以及电源单元，用于使直流电通过其上形成有导电膜的浸没结构和对电极。 通过将0.1〜5V相对于SCE的电位施加到这种防污染装置的浸没结构上，可以有效地控制附着在导电膜表面的水生生物，而不产生氯。 通过在浸没结构和对电极之间设置参考电极，能够以高精度控制施加到浸没结构的导电膜的电位。 作为形成在浸没结构体的基板上的导电膜，优选使用由金属氮化物构成的喷涂膜。

公开(公告)号：US5300128A

公开(公告)日：1994-04-05

申请号：US22124

申请日：1993-02-25

申请人： Tadashi Matsunaga ,  Hitoshi Wake ,  Mayumi Ono ,  Kiyoshi Hishinuma ,  Hironori Umetsu

发明人： Tadashi Matsunaga ,  Hitoshi Wake ,  Mayumi Ono ,  Kiyoshi Hishinuma ,  Hironori Umetsu

IPC分类号： A01H4/00 ,  C12N5/00 ,  C12N5/04 ,  C10L1/08 ,  C10L1/10

CPC分类号： C12N5/0025 ,  A01H4/006 ,  C12N5/04

摘要： A method of plant tissue culture which comprises culturing a tissue or an organ of a plant, a part of the same or cultured cells in a medium containing a culture filtrate and/or an extract of a photosynthetic procaryotic microorganism.Such a culturing method effectively proliferates the plant tissues and cultured cells, and also promotes the formation of adventive embryos, regeneration of the plant body and production of useful substances formed by that plant.Strains of cyanobacteria or photosynthetic bacteria are preferably used as the photosynthetic procaryotic microorganism.

摘要翻译： 一种植物组织培养方法，其包括在含有培养滤液和/或光合原核生物微生物提取物的培养基中培养植物的组织或器官，其一部分或培养细胞。 这种培养方法有效地增殖植物组织和培养细胞，并且还促进形成胚胎，植物体的再生和由该植物形成的有用物质的生产。 优选使用蓝细菌或光合细菌菌株作为光合原核生物。