公开(公告)号：US08877918B2

公开(公告)日：2014-11-04

申请号：US13362373

申请日：2012-01-31

申请人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

发明人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

IPC分类号： C07H21/00 ,  C12N15/10 ,  C12N1/06 ,  C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N1/06 ,  C12N15/1003 ,  C12Q2527/101 ,  C12Q2527/113

摘要： There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state.

摘要翻译： 提供了适用于较宽范围的微生物并且能够快速提取核酸的核酸提取方法。 核酸提取方法包括以下步骤：将细胞悬浮液引入容器，密封容器，并将加热器预热至不低于100℃的设定温度。此外，该方法包括使容器接触的步骤 加热器加热至设定温度，从而将容纳在容器中的细胞悬浮液加热至规定的最高温度至不低于100℃，容器保持密封状态。

公开(公告)号：US20120197008A1

公开(公告)日：2012-08-02

申请号：US13362373

申请日：2012-01-31

申请人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

发明人： Yoshihiro Ozeki ,  Akiyo Yamada ,  Nobuhiro Sasaki ,  Hitoshi Wake ,  Takeyuki Mogi ,  Tomoyuki Taguchi

IPC分类号： C07H1/08

CPC分类号： C12Q1/6806 ,  C12N1/06 ,  C12N15/1003 ,  C12Q2527/101 ,  C12Q2527/113

摘要： There is provided a nucleic acid extraction method applicable to microbes in a relatively wide range, and capable of rapidly extracting nucleic acid. The nucleic acid extraction method comprises the steps of introducing a cell suspension into a vessel, sealing the vessel, and preheating a heater up to a set temperature not lower than 100° C. Further, the method comprises the step of bringing the vessel into contact with the heater heated up to the set temperature, thereby heating the cell suspension housed in the vessel up to a prescribed highest temperature at not lower than 100° C. with the vessel held in a sealed state.

摘要翻译： 提供了适用于较宽范围的微生物并且能够快速提取核酸的核酸提取方法。 核酸提取方法包括以下步骤：将细胞悬浮液引入容器中，密封容器，并将加热器预热至不低于100℃的设定温度。此外，该方法包括使容器接触的步骤 加热器加热到设定温度，由此将容器保持在密封状态下，将容纳在容器中的细胞悬浮液加热至规定的最高温度至不低于100℃。

公开(公告)号：US20120135469A1

公开(公告)日：2012-05-31

申请号：US13389233

申请日：2010-01-21

申请人： Yoshihiro Ozeki ,  Nobuhiro Sasaki ,  Kazuo Nagasawa ,  Masayuki Tera ,  Yuki Matsuba ,  Haruka Nakamura ,  Yutaka Abe

发明人： Yoshihiro Ozeki ,  Nobuhiro Sasaki ,  Kazuo Nagasawa ,  Masayuki Tera ,  Yuki Matsuba ,  Haruka Nakamura ,  Yutaka Abe

IPC分类号： C12P19/44 ,  C12N5/10 ,  A01H5/00 ,  C07H15/207 ,  C12N15/54 ,  C12N9/10 ,  C12N15/63

CPC分类号： C12N9/1048 ,  C07H13/08 ,  C07H15/18 ,  C07H17/065 ,  C12P19/44 ,  C12P19/60

摘要： An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A): wherein R1 is independently selected from hydrogen, or C1-6 alkyl, C2-6 alkenyl, and C2-6 alkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO2, and SO2, n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a β bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.

摘要翻译： 本发明的目的是提供一种含糖糖核苷酸以外的糖供体化合物和能够使用糖核苷酸以外的糖供体化合物催化糖基转移反应的酶的供糖试剂。 本发明提供以下：含有式（A）化合物的给糖试剂：其中R 1独立地选自氢或C 1-6烷基，C 2-6烯基和C 2-6炔基，其中每个基团 未取代或被一个或多个选自OH，F，Cl，Br，I，CN，NO2和SO2的基团取代，n为0,1,2,3,4或5，m为0或1，X 代表通过＆bgr结合的单糖; 其端基异碳碳键; 能够使用糖供体催化糖基转移反应的糖基转移酶; 和包含编码糖基转移酶的DNA的糖基转移酶基因。

公开(公告)号：US08722868B2

公开(公告)日：2014-05-13

申请号：US13389233

申请日：2010-01-21

申请人： Yoshihiro Ozeki ,  Nobuhiro Sasaki ,  Kazuo Nagasawa ,  Masayuki Tera ,  Yuki Matsuba ,  Haruka Nakamura ,  Yutaka Abe

发明人： Yoshihiro Ozeki ,  Nobuhiro Sasaki ,  Kazuo Nagasawa ,  Masayuki Tera ,  Yuki Matsuba ,  Haruka Nakamura ,  Yutaka Abe

IPC分类号： C07H21/04 ,  C12N9/10 ,  C12N15/00 ,  C12N5/10

CPC分类号： C12N9/1048 ,  C07H13/08 ,  C07H15/18 ,  C07H17/065 ,  C12P19/44 ,  C12P19/60

摘要： An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A): wherein R1 is independently selected from hydrogen, or C1-6 alkyl, C2-6 alkenyl, and C2-6 alkynyl in which each of the groups is unsubstituted or substituted with one or more groups selected from OH, F, Cl, Br, I, CN, NO2, and SO2, n is 0, 1, 2, 3, 4 or 5, m is 0 or 1, and X represents a monosaccharide bound via a β bond on its anomeric carbon; a glycosyltransferase capable of catalyzing a glycosyl transfer reaction using the sugar donor; and a glycosyltransferase gene comprising DNA encoding the glycosyltransferase.

摘要翻译： 本发明的目的是提供一种含糖糖核苷酸以外的糖供体化合物和能够使用糖核苷酸以外的糖供体化合物催化糖基转移反应的酶的供糖试剂。 本发明提供以下：含有式（A）化合物的给糖试剂：其中R 1独立地选自氢或C 1-6烷基，C 2-6烯基和C 2-6炔基，其中每个基团 未取代或被一个或多个选自OH，F，Cl，Br，I，CN，NO2和SO2的基团取代，n为0,1,2,3,4或5，m为0或1，X 代表通过＆bgr结合的单糖; 其端基异碳碳键; 能够使用糖供体催化糖基转移反应的糖基转移酶; 和包含编码糖基转移酶的DNA的糖基转移酶基因。

公开(公告)号：US06258450B1

公开(公告)日：2001-07-10

申请号：US09357909

申请日：1999-07-21

申请人： Nobuhiro Sasaki ,  Yoshinao Takahashi ,  Katsuyuki Horie

发明人： Nobuhiro Sasaki ,  Yoshinao Takahashi ,  Katsuyuki Horie

IPC分类号： B32B300

CPC分类号： C04B35/4686 ,  H01B3/12 ,  H01G4/1227 ,  Y10T428/24926

摘要： The dielectric ceramic composition is composed of a sintered body of ceramic particles containing Ba2Ti5O11 as a major component. The ceramic particles may contain Ag in the form of solid solution at the rate of 0.2% to 3.0% by mole. the ceramic particles may further contain Ba2TiSi2O8 so the BaTi5O11/Ba2TiSi2O8 ratio is 1.5 to 9 BaTi5O11 to 1 Ba2TiSi2O8. The dielectric ceramic composition can be sintered at a low enough temperature to enable a highly conductive metal such as Ag, Cu or the like to be burned and sintered integrally.

摘要翻译： 电介质陶瓷组合物由以Ba2Ti5O11为主要成分的陶瓷粒子的烧结体构成。 陶瓷颗粒可以以0.2％至3.0％摩尔的比例含有固溶体形式的Ag。 陶瓷颗粒可能还含有Ba2TiSi2O8，因此BaTi5O11 / Ba2TiSi2O8的比例为1.5〜9 BaTi5O11〜1 Ba2TiSi2O8。 电介质陶瓷组合物可以在足够低的温度下烧结以使诸如Ag，Cu等的高导电性金属燃烧并整体烧结。

公开(公告)号：US5102925A

公开(公告)日：1992-04-07

申请号：US479900

申请日：1990-02-13

申请人： Yuji Suzuki ,  Tabito Nishimura ,  Nobuhiro Sasaki

发明人： Yuji Suzuki ,  Tabito Nishimura ,  Nobuhiro Sasaki

IPC分类号： C09D133/00 ,  C08L57/00 ,  C08L67/08 ,  C09D7/12 ,  C09D133/04 ,  C09D133/06 ,  C09D167/00 ,  C09D167/08

CPC分类号： C09D133/06 ,  C09D167/08 ,  C09D7/125 ,  C09D7/1266 ,  C09D7/1275 ,  C08L2205/18 ,  C08L57/00 ,  C08L67/08

摘要： An air-drying paint composition comprising (a) internally cross-linked polymer microparticles produced by emulsion polymerizing ethylenically unsaturated monomers including at least one cross-linking monomer, (b) a film-forming resin selected from the group consisting of an air-drying alkyd resin, an acrylic polymer and an acrylic-modified alkyd resin, and (c) a volatile organic solvent. The paint may be formulated in high solids content while retaining low viscosity.

公开(公告)号：US6103385A

公开(公告)日：2000-08-15

申请号：US64833

申请日：1998-04-23

申请人： Nobuhiro Sasaki

发明人： Nobuhiro Sasaki

IPC分类号： H01G4/12 ,  C04B35/462 ,  C04B35/468 ,  H01G4/012 ,  B32B9/00 ,  C04B35/46

CPC分类号： C04B35/462 ,  C04B35/4686 ,  C04B35/4688 ,  Y10T428/24355 ,  Y10T428/24917 ,  Y10T428/2913

摘要： The ceramic electronic part has a ceramic material and an electrode formed on the surface of the ceramic material. The ceramic material has rod-shaped particles formed projecting from the surface thereof, the rod-shaped particles having an aspect ratio (a long axis/short axis ratio) of from 3 to 30. The ceramic material is formed using a dielectric ceramic composition containing, as a major component, a composite oxide which, for example, comprises an oxide of barium (Ba), an oxide of titanium (Ti) an oxide of at least one rare earth element and one or both of an oxide of bismuth (Bi) and an oxide of lead (Pb). The ceramic electronic part can improve its characteristics at high frequency inherent in conventional ones which suffer from the remarkably worsening of their high frequency characteristics due to a rise in the specific resistance of the electrode when it attempts to improve the adherence strength of the electrode are made using an electrode paste to which a component capable of causing a chemical reaction with the ceramic material or which worsen their high frequency characteristics by the remaining of materials obtained by the reaction with a treatment liquid on the surface of the ceramic material when attempts are made to improve the adherence strength between the ceramic material and the electrode by chemical treatment.

摘要翻译： 陶瓷电子部件具有形成在陶瓷材料表面上的陶瓷材料和电极。 陶瓷材料具有从其表面突出形成的棒状颗粒，该棒状颗粒具有3至30的纵横比（长轴/短轴比）。陶瓷材料使用包含 作为主要成分的复合氧化物，例如包含钡（Ba）的氧化物，钛（Ti）的氧化物，至少一种稀土元素的氧化物和铋的氧化物（Bi ）和铅（Pb）的氧化物。 陶瓷电子部件可以提高其在常规的固有的高频下的特性，其中当试图提高电极的粘附强度时，由于电极的电阻率的上升而使其高频特性显着恶化 使用能够与陶瓷材料发生化学反应的成分的电极糊料，或者通过与陶瓷材料的表面上的处理液反应而获得的剩余材料使其高频特性恶化， 通过化学处理提高陶瓷材料与电极之间的粘附强度。

公开(公告)号：US07148170B2

公开(公告)日：2006-12-12

申请号：US10200121

申请日：2002-07-23

申请人： Nobuhiro Sasaki ,  Sadayoshi Kato ,  Kunihiro Matsushita ,  Makoto Miyashiro ,  Hitoshi Kikuchi ,  Tadashi Itoi

发明人： Nobuhiro Sasaki ,  Sadayoshi Kato ,  Kunihiro Matsushita ,  Makoto Miyashiro ,  Hitoshi Kikuchi ,  Tadashi Itoi

IPC分类号： C04B35/622

CPC分类号： C04B35/638 ,  C04B35/62204 ,  C04B35/6261 ,  C04B35/6264 ,  C04B35/62695 ,  C04B2235/725

摘要： Recycled ceramic slurry is produced by a method including the steps: dissolving the waste material of unfired ceramic in a solvent to form a waste solution; and adjusting a composition of the waste solution to thereby produce the recycled ceramic slurry. If the waste material of unfired ceramic includes a metal component, the metal component may be removed therefrom before the step of adjusting the composition of the waste solution.

公开(公告)号：US5994253A

公开(公告)日：1999-11-30

申请号：US110417

申请日：1998-07-06

申请人： Nobuhiro Sasaki ,  Katsuyuki Horie ,  Hirokazu Chazono

发明人： Nobuhiro Sasaki ,  Katsuyuki Horie ,  Hirokazu Chazono

IPC分类号： C04B35/00 ,  C04B35/46 ,  C04B35/468 ,  H01B3/12 ,  H01G4/12

CPC分类号： C04B35/4686 ,  C04B35/4688 ,  H01G4/1227

摘要： A dielectric ceramic composition is comprised of a crystal structure phase of a tungsten bronze form, one titanate-barium phase or more titanate-barium phases selected from Ba.sub.2 Ti.sub.9 O.sub.20, BaTi.sub.2 O.sub.5, BaTi.sub.4 O.sub.9 and Ba.sub.4 Ti.sub.13 O.sub.30, and a fine crystal phase comprising an oxide of each of at least B, Ag and Mn, in which the crystal structure phase of the tungsten bronze form is formed with a limited amount of a compound oxide of Ba, Nd and Ti as a basic component and contains a limited amount of each of an oxide of at least Bi, Pb, Zn and Si. The dielectric ceramic composition can be fired at a temperature of 920.degree. C. or lower and sintered forming into a ceramic electronic part which has remarkably high dielectric characteristics, such as the dielectric constant of 60 or higher, the Q factor of 1,000 or higher, and the temperature coefficient .tau..epsilon.r of the relative dielectric constant .epsilon.r of .+-.60 ppm/.degree. C. or smaller. The ceramic electronic parts for high frequency are further provided with high electrical features and prepared at low costs.

摘要翻译： 电介质陶瓷组合物由选自Ba2Ti9O20，BaTi2O5，BaTi4O9和Ba4Ti13O30的钨青铜型晶体结构相，一种钛酸钡 - 钡相或更多种钛酸钡 - 钡相组成，以及包含各自的氧化物的细晶相 最少的B，Ag和Mn，其中青铜形式的晶体结构相形成有限量的作为碱性组分的Ba，Nd和Ti的复合氧化物，并且含有有限量的每种氧化物的 最少的Bi，Pb，Zn和Si。 电介质陶瓷组合物可以在920℃以下的温度下烧结，烧结成陶瓷电子部件，其电介质特性如介电常数为60以上，Q为1000以上， 相对介电常数εr的温度系数τεr为+/- 60ppm /℃以下。 高频陶瓷电子部件还具有较高的电气特性，且成本低廉。