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公开(公告)号：US5300128A
公开(公告)日：1994-04-05
申请号：US22124
申请日：1993-02-25
CPC分类号： C12N5/0025 , A01H4/006 , C12N5/04
摘要： A method of plant tissue culture which comprises culturing a tissue or an organ of a plant, a part of the same or cultured cells in a medium containing a culture filtrate and/or an extract of a photosynthetic procaryotic microorganism.Such a culturing method effectively proliferates the plant tissues and cultured cells, and also promotes the formation of adventive embryos, regeneration of the plant body and production of useful substances formed by that plant.Strains of cyanobacteria or photosynthetic bacteria are preferably used as the photosynthetic procaryotic microorganism.
摘要翻译： 一种植物组织培养方法，其包括在含有培养滤液和/或光合原核生物微生物提取物的培养基中培养植物的组织或器官，其一部分或培养细胞。 这种培养方法有效地增殖植物组织和培养细胞，并且还促进形成胚胎，植物体的再生和由该植物形成的有用物质的生产。 优选使用蓝细菌或光合细菌菌株作为光合原核生物。
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公开(公告)号：US5217892A
公开(公告)日：1993-06-08
申请号：US908894
申请日：1992-07-08
CPC分类号： A01H4/006 , C12N5/0025 , C12N5/04
摘要： A method of plant tissue culture which comprises culturing a tissue or an organ of a plant, a part of the same or cultured cells in a medium containing a culture filtrate and/or an extract of a photosynthetic procaryotic microorganism.Such a culturing method effectively proliferates the plant tissues and cultured cells, and also promotes the formation of adventive embryos, regeneration of the plant body and production of useful substances formed by that plant.Strains of cyanobacteria or photosynthetic bacteria are preferably used as the photosynthetic procaryotic microorganism.
摘要翻译： PCT No.PCT / JP89 / 00741 Sec。 371 1990年3月21日 102（e）1990年3月21日PCT PCT日期：1989年7月25日PCT公布。 出版物WO90 / 00855 1990年2月8日。一种植物组织培养方法，其包括在含有培养滤液和/或光合原核生物微生物提取物的培养基中培养植物的组织或器官，其一部分或培养细胞 。 这种培养方法有效地增殖植物组织和培养细胞，并且还促进形成胚胎，植物体的再生和由该植物形成的有用物质的生产。 优选使用蓝细菌或光合细菌菌株作为光合原核生物。
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3.发明授权公开(公告)号：US06197168B1
公开(公告)日：2001-03-06
申请号：US09426658
申请日：1999-10-25
申请人： Tadashi Matsunaga , Tsuruo Nakayama , Hitoshi Wake , Kin-ichi Ozawa , Noriyuki Nakamura , Nobuyuki Murakami , Hiromichi Takahashi , Toshihiro Takimoto , Hideo Kadoi
发明人： Tadashi Matsunaga , Tsuruo Nakayama , Hitoshi Wake , Kin-ichi Ozawa , Noriyuki Nakamura , Nobuyuki Murakami , Hiromichi Takahashi , Toshihiro Takimoto , Hideo Kadoi
IPC分类号： C23F1300
CPC分类号： B63B59/04 , C02F1/48 , C02F2303/04 , C23F13/00
摘要： An electrochemical stain prevention apparatus of a submerged structure comprising a submerged structure of which at least the stain prevention surface is formed of a conductive film that does not generate chlorine even by applying a potential of 5 V vs. SCE or less, a counter electrode located so as not to contact with the submerged structure, and a power supply unit for passing a direct current through the submerged structure having the conductive film formed thereon and the counter electrode. Aquatic organisms adhered to the surface of the conductive film can effectively be controlled by applying a potential of from 0.1 to 5 V vs. SCE to the submerged structure of such a stain prevention apparatus without generating chlorine. A potential applied to the conductive film of the submerged structure can be controlled with good accuracy by disposing a reference electrode between the submerged structure and the counter electrode. As the conductive film formed on the substrate of the submerged structure, a sprayed coating film made of a metal nitride can preferably be used.
摘要翻译： 一种浸没结构的电化学防污染装置，包括浸没结构，其至少防污染表面由不产生氯的导电膜形成，即使通过施加5V与SCE以下的电位，也可以使用对置电极 以便不与浸没结构接触;以及电源单元，用于使直流电通过其上形成有导电膜的浸没结构和对电极。 通过将0.1〜5V相对于SCE的电位施加到这种防污染装置的浸没结构上，可以有效地控制附着在导电膜表面的水生生物，而不产生氯。 通过在浸没结构和对电极之间设置参考电极，能够以高精度控制施加到浸没结构的导电膜的电位。 作为形成在浸没结构体的基板上的导电膜，优选使用由金属氮化物构成的喷涂膜。
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公开(公告)号：US20110057145A1
公开(公告)日：2011-03-10
申请号：US12876562
申请日：2010-09-07
申请人： Tadashi Matsunaga , Tsuyoshi Tanaka , Keiichi Hatakeyama , Takeo Tanaami , Hitoshi Wake , Tomoyuki Taguchi , Takeyuki Mogi
发明人： Tadashi Matsunaga , Tsuyoshi Tanaka , Keiichi Hatakeyama , Takeo Tanaami , Hitoshi Wake , Tomoyuki Taguchi , Takeyuki Mogi
IPC分类号： H01F1/00
CPC分类号： H01F1/42
摘要： There is provided a method for preparing dendrimer-modified magnetic fine particles wherein such particles can be made within a shorter time and more inexpensively than in the above-stated prior art processes and lot-to-lot variations in properties are lessened. The method for preparing dendrimer-fixed magnetic fine particles comprises the steps of providing magnetic particles having a functional group at a surface thereof, providing a dendrimer having a functional group at a base end portion thereof and synthesized to a desired generation and binding the functional group of the magnetic particles and the functional group of the dendrimer directly or indirectly through a crosslinking agent.
摘要翻译： 提供了一种制备树枝状聚合物改性的磁性细颗粒的方法，其中这种颗粒可以在比上述现有技术方法更短的时间和更廉价的情况下制备，并且批次间的性质变化减少。 制备树枝状大分子固定磁性微粒的方法包括以下步骤：在其表面提供具有官能团的磁性颗粒，提供在其基端部分具有官能团的树枝状聚合物，并合成所需的一代并结合官能团 的磁性颗粒和树枝状聚合物的官能团直接或间接地通过交联剂。
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公开(公告)号：US07834139B2
公开(公告)日：2010-11-16
申请号：US10593514
申请日：2005-03-14
申请人： Hiroshi Matsui , Tadashi Matsunaga
发明人： Hiroshi Matsui , Tadashi Matsunaga
CPC分类号： B82Y30/00 , B82Y5/00 , B82Y15/00 , B82Y25/00 , C01G49/08 , C01P2004/04 , C01P2004/13 , C01P2004/64 , C01P2006/42 , C07K14/195 , G01N33/56911 , G01N33/588 , H01F1/0081 , Y10S977/838 , Y10S977/904 , Y10T428/32
摘要： A magnetic nanotube includes bacterial magnetic nanocrystals contacted onto a nanotube which absorbs the nanocrystals. The nanocrystals are contacted on at least one surface of the nanotube. A method of fabricating a magnetic nanotube includes synthesizing the bacterial magnetic nanocrystals, which have an outer layer of proteins. A nanotube provided is capable of absorbing the nanocrystals and contacting the nanotube with the nanocrystals. The nanotube is preferably a peptide bolaamphiphile. A nanotube solution and a nanocrystal solution including a buffer and a concentration of nanocrystals are mixed. The concentration of nanocrystals is optimized, resulting in a nanocrystal to nanotube ratio for which bacterial magnetic nanocrystals are immobilized on at least one surface of the nanotubes. The ratio controls whether the nanocrystals bind only to the interior or to the exterior surfaces of the nanotubes. Uses include cell manipulation and separation, biological assay, enzyme recovery, and biosensors.
摘要翻译： 磁性纳米管包括接触纳米管的细菌磁性纳米晶体，其吸收纳米晶体。 纳米晶体在纳米管的至少一个表面上接触。 制造磁性纳米管的方法包括合成具有蛋白质外层的细菌磁性纳米晶体。 所提供的纳米管能够吸收纳米晶体并使纳米管与纳米晶体接触。 纳米管优选为多肽多肽。 混合包含缓冲液和纳米晶体浓度的纳米管溶液和纳米晶体溶液。 纳米晶体的浓度被优化，导致纳米晶体与纳米管的比率，细菌磁性纳米晶体固定在纳米管的至少一个表面上。 该比例控制纳米晶体是否仅结合到纳米管的内部或外部表面。 用途包括细胞操作和分离，生物测定，酶回收和生物传感器。
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公开(公告)号：US20050130191A1
公开(公告)日：2005-06-16
申请号：US10928183
申请日：2004-08-30
IPC分类号： G01N33/53 , C07H1/08 , C07H21/04 , C12M1/00 , C12N15/09 , C12Q1/68 , G01N33/50 , G01N33/543 , G01N33/566 , G01N33/58
CPC分类号： G01N33/54353 , C07H21/04 , C12Q1/682 , C12Q1/6837 , Y10T436/143333 , C12Q2565/629 , C12Q2565/501
摘要： The present invention provides a dendrimer-based biochip, wherein a flow channel through which a solution containing biopolymer molecules is flowed is formed in the substrate of the biochip, a plurality of dendrimer molecules one end of each of which is bound to the walls of the flow channel are formed thereon, and probe biopolymer or antibody molecules are bound to the tips of the dendrimer molecules so that, if the probe biopolymer molecules are bound, then target biopolymer molecules can be captured by means of a complementary combination and, if the antibody molecules are bound, then protein can be extracted by means of antigen-antibody reaction, whereby biopolymers can be retrieved in a highly efficient manner.
摘要翻译： 本发明提供了一种基于树状聚合物的生物芯片，其中在生物芯片的基底中形成有流过含有生物聚合物分子的溶液的流动通道，多个树枝状大分子的一端结合到生物芯片的壁上 在其上形成流动通道，并且探针生物聚合物或抗体分子结合到树状聚合物分子的末端，使得如果探针生物聚合物分子结合，则靶生物聚合物分子可以通过互补组合捕获，并且如果抗体 分子结合，则可以通过抗原 - 抗体反应提取蛋白质，从而以高效的方式检索生物聚合物。
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公开(公告)号：US6033878A
公开(公告)日：2000-03-07
申请号：US122632
申请日：1998-07-27
申请人： Tadashi Matsunaga
发明人： Tadashi Matsunaga
IPC分类号： C07K14/195 , C12N11/00 , C12N15/62 , G01N33/543 , G01N33/554 , C12P21/04 , C07H21/04 , C07K1/00 , C12N1/20 , C12N15/00
CPC分类号： G01N33/54326 , C07K14/195 , C12N11/00 , C12N15/62 , G01N33/554 , C07K2319/00 , G01N2333/195
摘要： A fusion DNA sequence, which is obtained by fusing a gene coding for another useful protein to a fragment of a magA gene coding for a protein bound to an organic membrane for covering magnetic particles produced in cells of a magnetic bacterium AMB-1, is expressed in the magnetic bacterium to obtain the protein in a state of being bound to the magnetic particles. According to the present invention, useful proteins such as enzymes and antibodies can be stably obtained in a state of being bound to the organic membrane of the magnetic particles only by cultivating a transformed magnetic bacterium, and separating the magnetic particles produced in cells, without any necessity to perform a treatment such as immobilization. The functional protein immobilized on the magnetic particles can be magnetically controlled. Thus the function can be efficiently exhibited at a desired topical position. Magnetic particles to which a desired protein is bound can be semipermanently produced only by maintaining and cultivating an identical bacterial strain. Since the protein is produced on the magnetic particles, an objective protein can be magnetically separated and recovered in a short period of time. Thus it is possible to perform efficient separation and purification.
摘要翻译： 通过将编码另一有用蛋白质的基因融合到编码与有机膜结合的蛋白质的magA基因的片段以覆盖在细菌AMB-1的细胞中产生的磁性颗粒而获得的融合DNA序列被表达 在磁性细菌中以获得与磁性颗粒结合的状态的蛋白质。 根据本发明，只有通过培养转化的磁性细菌才能稳定地获得有用的蛋白质，例如酶和抗体，只要通过培养转化的磁性细菌才能与磁性颗粒的有机膜结合，并且分离细胞中产生的磁性颗粒 需要进行固定等处理。 固定在磁性颗粒上的功能性蛋白质可以被磁控制。 因此，可以在期望的局部位置有效地显示该功能。 通过保持和培养相同的细菌菌株，可以半永久地产生所需蛋白质结合的磁性颗粒。 由于蛋白质是在磁性颗粒上产生的，所以目标蛋白质可以在短时间内被磁分离和回收。 因此，可以进行有效的分离和纯化。
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公开(公告)号：US5476797A
公开(公告)日：1995-12-19
申请号：US135580
申请日：1993-10-13
申请人： Tadashi Matsunaga
发明人： Tadashi Matsunaga
IPC分类号： G01N31/22 , G01N33/48 , G01N33/569 , G01N33/567 , G01N21/64 , G01N27/404 , G01N27/48
CPC分类号： G01N33/48 , G01N31/22 , G01N33/56972 , Y10S436/806 , Y10S436/808
摘要： Processes for detecting a sensitized leukocyte or an antigen in a liquid sample are described. One of the processes contains the steps of: adding a known antigen to the liquid sample, or the liquid sample to a known sensitized leukocyte; applying an electric potential between a working electrode immersed in a mixture of the known antigen or the known leukocytes and the liquid sample, and a counter electrode, to generate an electric current therein; and measuring an amount of the electric current generated. Further, another process contains the steps of: adding a known antigen to said liquid sample, or the liquid sample to a known sensitized leukocyte; and measuring an amount of serotonin or histamine released thereby. Still further, apparatuses for carrying out the above processes are also described.
摘要翻译： 描述了用于检测液体样品中敏化白细胞或抗原的方法。 其中一个过程包括以下步骤：向液体样品或液体样品中加入已知抗原至已知致敏白细胞; 在浸没在已知抗原或已知白细胞的混合物中的工作电极与液体样品之间施加电位和相对电极，以在其中产生电流; 并测量产生的电流量。 此外，另一方法包括以下步骤：向所述液体样品或液体样品中加入已知抗原至已知致敏白细胞; 并测量由此释放的5-羟色胺或组胺的量。 此外，还描述了用于执行上述处理的装置。
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公开(公告)号：US08003562B2
公开(公告)日：2011-08-23
申请号：US12442533
申请日：2007-09-14
IPC分类号： B01J21/08 , B01J21/06 , B01J23/02 , C03C12/02 , C04B35/46 , C04B35/465 , C04B35/468 , C04B35/47
CPC分类号： B01J35/004 , B01J21/063 , B01J21/08 , B01J23/002 , B01J23/02 , B01J35/008 , B01J35/06 , B01J2523/00 , C02F1/32 , C02F1/725 , C02F2305/10 , C04B35/14 , C04B35/6224 , C04B35/62259 , C04B2235/3213 , C04B2235/3215 , C04B2235/3232 , C04B2235/3236 , C04B2235/3272 , C04B2235/3418 , C04B2235/441 , C04B2235/449 , C04B2235/483 , C04B2235/775 , C04B2235/80 , D01F9/08 , B01J2523/24 , B01J2523/47 , B01J2523/25
摘要： A silica base composite photocatalyst that has appropriate water purification capability, inhibiting precipitation of metal oxides; and a process for producing the same. The silica base composite photocatalyst is one composed mainly of a composite oxide phase consisting of an oxide phase (first phase) composed mainly of silica component and a titania phase (second phase) wherein the ratio of presence of the second phase increases aslope toward the surface layer, characterized in that at least one metal oxide selected from among strontium titanate and barium titanate is contained in the second phase.
摘要翻译： 具有适当水净化能力，抑制金属氧化物沉淀的二氧化硅基复合光催化剂; 及其制造方法。 二氧化硅基复合光催化剂主要由复合氧化物相组成，该复合氧化物相由主要由二氧化硅组分组成的氧化物相（第一相）和二氧化钛相（第二相）组成，其中第二相的存在比例向斜面增加 其特征在于，在所述第二相中包含选自钛酸锶和钛酸钡中的至少一种金属氧化物。
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10.发明申请公开(公告)号：US20100087311A1
公开(公告)日：2010-04-08
申请号：US12442533
申请日：2007-09-14
IPC分类号： B01J21/08
CPC分类号： B01J35/004 , B01J21/063 , B01J21/08 , B01J23/002 , B01J23/02 , B01J35/008 , B01J35/06 , B01J2523/00 , C02F1/32 , C02F1/725 , C02F2305/10 , C04B35/14 , C04B35/6224 , C04B35/62259 , C04B2235/3213 , C04B2235/3215 , C04B2235/3232 , C04B2235/3236 , C04B2235/3272 , C04B2235/3418 , C04B2235/441 , C04B2235/449 , C04B2235/483 , C04B2235/775 , C04B2235/80 , D01F9/08 , B01J2523/24 , B01J2523/47 , B01J2523/25
摘要： A silica base composite photocatalyst that has appropriate water purification capability, inhibiting precipitation of metal oxides; and a process for producing the same. The silica base composite photocatalyst is one composed mainly of a composite oxide phase consisting of an oxide phase (first phase) composed mainly of silica component and a titania phase (second phase) wherein the ratio of presence of the second phase increases aslope toward the surface layer, characterized in that at least one metal oxide selected from among strontium titanate and barium titanate is contained in the second phase.
摘要翻译： 具有适当水净化能力，抑制金属氧化物沉淀的二氧化硅基复合光催化剂; 及其制造方法。 二氧化硅基复合光催化剂主要由复合氧化物相组成，该复合氧化物相由主要由二氧化硅组分组成的氧化物相（第一相）和二氧化钛相（第二相）组成，其中第二相的存在比例向斜面增加 其特征在于，在所述第二相中包含选自钛酸锶和钛酸钡中的至少一种金属氧化物。
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