公开(公告)号：US20060269449A1

公开(公告)日：2006-11-30

申请号：US11420343

申请日：2006-05-25

申请人： Takafumi Nakamura ,  Yusuke Osaka ,  Kazuaki Kakehi

发明人： Takafumi Nakamura ,  Yusuke Osaka ,  Kazuaki Kakehi

IPC分类号： B32B27/04 ,  G01N33/00

CPC分类号： C13B20/02 ,  C13B20/14

摘要： A reaction tank 15 for heating a sample solution containing glycoprotein in an alkali solution to separate sugar chains, and two cartridges 17a and 17b filled with ion exchange resin are provided, and the sample solution transported from the reaction tank 15 is subjected to neutralization treatment by passing the sample solution through the cartridges. The cartridges 17a and 17b are connected in parallel by two flow channel switching valves 16a and 16b. By switching the valves 16a and 16b, selection of a cartridge to be used for the neutralization treatment is made, and at the same time, acid and water are passed through the cartridge which is not in use, in order to allow regeneration of the ion exchange resin in the cartridge.

摘要翻译： 用于加热含碱性溶液中含有糖蛋白的样品溶液以分离糖链的反应槽15，以及填充有离子交换树脂的两个盒17a和17b，从反应槽15输送的样品溶液进行中和 通过将样品溶液通过墨盒进行处理。 盒17a和17b由两个流路切换阀16a和16b并联连接。 通过切换阀门16a和16b，进行中和处理用的墨盒的选择，并且同时使酸和水通过未使用的墨盒，以便允许再生 盒中的离子交换树脂。

公开(公告)号：US20070178581A1

公开(公告)日：2007-08-02

申请号：US11607025

申请日：2006-12-01

申请人： Yusuke Osaka ,  Morimasa Hayashi

发明人： Yusuke Osaka ,  Morimasa Hayashi

IPC分类号： G01N33/558 ,  C12M3/00

CPC分类号： G01N33/558

摘要： An screening method and apparatus for online identification of a reactive compound bonding with a target compound includes a first dimension analysis step whereby a sample including a target compound and a reactive compound are injected together into a sample injection port. The sample is led to a first dimension column using a first dimension mobile phase and is separated into components. An isolation step isolates the sample portion into a loop passage. A reactive compound concentration step includes leading the reaction products into a trap column that selectively traps the reactive compound while dissociating it from the reaction products isolated by the isolation step. A second dimension analysis step leads the sample trapped in the trap column to a second dimension column using a second dimension mobile phase.

摘要翻译： 用于在线鉴定与目标化合物键合的反应性化合物的筛选方法和装置包括第一维度分析步骤，其中将包含目标化合物和反应性化合物的样品一起注入样品注入口。 使用第一维流动相将样品引导到第一维柱，并将其分成组分。 隔离步骤将样品部分隔离成环通道。 反应性化合物浓缩步骤包括引导反应产物进入捕集塔，该捕集塔选择性捕集反应性化合物，同时使其与通过分离步骤分离的反应产物解离。 第二维分析步骤使用第二维移动相将陷阱中捕获的样品引导到第二维列。

公开(公告)号：US20050218055A1

公开(公告)日：2005-10-06

申请号：US11088930

申请日：2005-03-25

申请人： Morimasa Hayashi ,  Yosuke Iwata ,  Yusuke Osaka

发明人： Morimasa Hayashi ,  Yosuke Iwata ,  Yusuke Osaka

IPC分类号： G01N30/46 ,  B01D15/08 ,  G01N30/08 ,  G01N30/26 ,  G01N30/72 ,  G01N30/74 ,  G01N30/78

CPC分类号： G01N30/463 ,  G01N30/461 ,  G01N30/468 ,  G01N2030/085 ,  B01D15/361 ,  B01D15/325

摘要： A two-dimensional liquid chromatograph includes a first-dimension separation channel for guiding a sample injected from a sample injection part to a first-dimension analysis column using a first-dimension analysis mobile phase for separation; two trap columns; an analysis channel for guiding components retained in the trap columns to a second-dimension analysis column using a second-dimension analysis mobile phase for analysis; and a channel switching mechanism. The switching mechanism connects the first-dimension separation channel to one of the trap columns and connecting the analysis channel to the other of the trap columns, and also switches connections between the trap columns and the channels.

摘要翻译： 二维液相色谱仪包括：第一维分离通道，用于使用第一维分析流动相分离从第一维分析柱引入从样品注入部分注入的样品; 两个陷阱列; 分析通道，用于使用二维分析流动相将捕集塔中保留的组分引导到二维分析柱进行分析; 和信道切换机制。 切换机构将第一维分离通道连接到其中一个陷阱列，并将分析通道连接到另一个陷阱列，还可以切换陷阱列和通道之间的连接。

公开(公告)号：US07214313B2

公开(公告)日：2007-05-08

申请号：US11088930

申请日：2005-03-25

申请人： Morimasa Hayashi ,  Yosuke Iwata ,  Yusuke Osaka

发明人： Morimasa Hayashi ,  Yosuke Iwata ,  Yusuke Osaka

IPC分类号： B01D15/08

CPC分类号： G01N30/463 ,  G01N30/461 ,  G01N30/468 ,  G01N2030/085 ,  B01D15/361 ,  B01D15/325

摘要： A two-dimensional liquid chromatograph includes a first-dimension separation channel for guiding a sample injected from a sample injection part to a first-dimension analysis column using a first-dimension analysis mobile phase for separation; two trap columns; an analysis channel for guiding components retained in the trap columns to a second-dimension analysis column using a second-dimension analysis mobile phase for analysis; and a channel switching mechanism. The switching mechanism connects the first-dimension separation channel to one of the trap columns and connecting the analysis channel to the other of the trap columns, and also switches connections between the trap columns and the channels.

摘要翻译： 二维液相色谱仪包括：第一维分离通道，用于使用第一维分析流动相分离从第一维分析柱引入从样品注入部分注入的样品; 两个陷阱列; 分析通道，用于使用二维分析流动相将捕集塔中保留的组分引导到二维分析柱进行分析; 和信道切换机制。 切换机构将第一维分离通道连接到其中一个陷阱列，并将分析通道连接到另一个陷阱列，还可以切换陷阱列和通道之间的连接。

公开(公告)号：US20060210437A1

公开(公告)日：2006-09-21

申请号：US11371734

申请日：2006-03-09

申请人： Yusuke Osaka

发明人： Yusuke Osaka

IPC分类号： G01N30/00

CPC分类号： G01N30/466 ,  G01N30/468 ,  G01N2030/027

摘要： Different lectins are immobilized in four columns, respectively. A sample containing a sugar chain is injected from a sample injection section, sent to one of the columns through a first flow path switching valve and a second or third flow path switching valve. Then, an eluate of the column is sent to a detector through a fourth flow path switching valve and is detected. Simultaneously, a cleaning solution is sent to one of columns, which differs from the column performing the analysis, through the first flow path switching valve and the second or third flow path switching valve. After the column is cleaned, the cleaning solution is discharged to a drain through the fourth flow path switching valve. Measurements are performed in the four columns sequentially, and measurement data are inputted into an arithmetic control device. Subsequently, data representing interactions between the sugar chain and the lectins are obtained.

摘要翻译： 不同的凝集素分别固定在四列中。 从样品注射部分注入含有糖链的样品，通过第一流路切换阀和第二或第三流路切换阀送到一列。 然后，柱的洗脱液通过第四流路切换阀送到检测器，并被检测。 同时，通过第一流路切换阀和第二或第三流路切换阀，将清洗液送到与执行分析的列不同的列之一。 在柱被清洁之后，清洁溶液通过第四流路切换阀排出到排水口。 依次对四列执行测量，并将测量数据输入到运算控制装置。 随后，获得表示糖链与凝集素之间相互作用的数据。