公开(公告)号：US08882012B2

公开(公告)日：2014-11-11

申请号：US13217437

申请日：2011-08-25

申请人： Yuji Haraguchi ,  Tatsuya Shimizu ,  Teruo Okano ,  Takahiro Shioyama ,  Akane Suzuki ,  Sunao Takeda

发明人： Yuji Haraguchi ,  Tatsuya Shimizu ,  Teruo Okano ,  Takahiro Shioyama ,  Akane Suzuki ,  Sunao Takeda

IPC分类号： B02C18/00 ,  B02C13/28 ,  C12M1/33 ,  G01N1/28 ,  C12M1/00

CPC分类号： G01N1/286 ,  C12M41/02 ,  C12M45/02 ,  C12M47/04 ,  G01N2001/2866

摘要： A cell isolation apparatus includes: a chamber body which includes a chamber into which tissue is to be introduced; an isolation member which is moved in the chamber and which is to collide with the tissue to isolate a cell; and a controlling portion which controls an operation of the isolation member in the chamber body.

摘要翻译： 细胞分离装置包括：腔体，其包括要被导入组织的腔室; 隔离构件，其在腔室中移动并与组织碰撞以隔离细胞; 以及控制部分，其控制室主体中的隔离构件的操作。

公开(公告)号：US08420028B2

公开(公告)日：2013-04-16

申请号：US13214774

申请日：2011-08-22

申请人： Akane Suzuki ,  Takahiro Shioyama ,  Sunao Takeda

发明人： Akane Suzuki ,  Takahiro Shioyama ,  Sunao Takeda

IPC分类号： A61B10/00

CPC分类号： G01N1/286 ,  G01N1/38 ,  G01N15/14 ,  G01N2001/085 ,  G01N2001/2866

摘要： A cell isolation apparatus includes: a cylinder member that is inserted into a container into which a reagent is to be introduced; a net which is stretched over the cylinder member; a tissue acquiring unit that is projected from the cylinder member; and a bottom lid portion that is configured by a bottomed pipe in which a first hole that communicates an interior with an exterior is formed, and that is fitted to a bottom portion of the cylinder member in a state where the net and the tissue acquiring unit are accommodated.

摘要翻译： 细胞隔离装置包括：插入到待引入试剂的容器中的圆筒构件; 在气缸构件上拉伸的网; 组织获取单元，其从所述圆筒构件突出; 以及底盖部，其由形成有与内部连通的第一孔的有底管构成，并且在所述网和所述组织获取单元的状态下嵌合到所述筒构件的底部， 被容纳。

公开(公告)号：US09970872B2

公开(公告)日：2018-05-15

申请号：US13217338

申请日：2011-08-25

申请人： Takahiro Shioyama ,  Akane Suzuki ,  Sunao Takeda

发明人： Takahiro Shioyama ,  Akane Suzuki ,  Sunao Takeda

IPC分类号： G01N33/48 ,  G01N21/64 ,  G01N15/14 ,  G01N15/10

CPC分类号： G01N21/6428 ,  G01N15/1459 ,  G01N2015/1006 ,  G01N2015/1402 ,  G01N2015/1488

摘要： A cell analyzer includes: a measuring portion which measures cells that are nuclear stained; a displaying portion which displays a histogram of a fluorescence intensity by using a result of the measurement by the measuring portion; and a determining unit which obtains a number of strong-area cells that are distributed in an area where the fluorescence intensity is stronger than normal cells, and which determines a malignancy grade of cancer based on the number of strong-area cells and the histogram.

公开(公告)号：US09163209B2

公开(公告)日：2015-10-20

申请号：US13541183

申请日：2012-07-03

申请人： Takahiro Shioyama ,  Akane Suzuki

发明人： Takahiro Shioyama ,  Akane Suzuki

IPC分类号： B01L3/02 ,  C12M3/00 ,  C12M1/00 ,  C12M1/33 ,  B01L3/00 ,  G01F25/00 ,  G01N35/10

CPC分类号： C12M47/04 ,  B01L3/021 ,  B01L3/502 ,  B01L2300/047 ,  B01L2300/0681 ,  B01L2400/0487 ,  C12M45/02 ,  G01N2035/103 ,  G01N2035/1053

摘要： A pipette member to be attachable to a container capable of accommodating a tissue and a liquid, is used for isolating cells from the tissue by stirring, and includes: a cylinder member which includes a first opening that is to be accommodated in the container, and a second opening that is to be connected to a cell isolation apparatus, and in which a passage for communicating the first opening with the second opening is defined; and a filter member which is disposed inside the cylinder member, and which separates the passage into a first part that includes the first opening, and a second part that includes the second opening.

摘要翻译： 可附接到容纳组织和液体的容器的移液管构件用于通过搅拌从组织中分离细胞，并且包括：气缸构件，其包括容纳在容器中的第一开口，以及 要连接到电池隔离装置的第二开口，其中限定了用于使第一开口与第二开口连通的通道; 以及过滤构件，其设置在所述缸构件的内部，并且将所述通道分离成包括所述第一开口的第一部分，以及包括所述第二开口的第二部分。

公开(公告)号：US08821815B2

公开(公告)日：2014-09-02

申请号：US13542459

申请日：2012-07-05

申请人： Takahiro Shioyama ,  Akane Suzuki ,  Teruo Okano ,  Tatsuya Shimizu ,  Yuji Haraguchi

发明人： Takahiro Shioyama ,  Akane Suzuki ,  Teruo Okano ,  Tatsuya Shimizu ,  Yuji Haraguchi

IPC分类号： B01D35/00 ,  C12M1/33

CPC分类号： C12M45/02

摘要： A cell isolation instrument includes: a first container which has an opening in an upper portion; a filtration member which defines at least a part of a lower portion of the first container; an isolation member which is movably housed in the first container to collide with a tissue, thereby isolating cells; and a second container which houses the first container in a manner that the first container can be taken out.

摘要翻译： 电池隔离器包括：第一容器，其在上部具有开口; 限定所述第一容器的下部的至少一部分的过滤构件; 隔离构件，其可移动地容纳在第一容器中以与组织碰撞，从而隔离单元; 以及容纳第一容器的第二容器，其中第一容器可以被取出。

公开(公告)号：US20130017129A1

公开(公告)日：2013-01-17

申请号：US13541183

申请日：2012-07-03

申请人： Takahiro Shioyama ,  Akane Suzuki

发明人： Takahiro Shioyama ,  Akane Suzuki

IPC分类号： B01L3/02

CPC分类号： C12M47/04 ,  B01L3/021 ,  B01L3/502 ,  B01L2300/047 ,  B01L2300/0681 ,  B01L2400/0487 ,  C12M45/02 ,  G01N2035/103 ,  G01N2035/1053

摘要： A pipette member to be attachable to a container capable of accommodating a tissue and a liquid, is used for isolating cells from the tissue by stirring, and includes: a cylinder member which includes a first opening that is to be accommodated in the container, and a second opening that is to be connected to a cell isolation apparatus, and in which a passage for communicating the first opening with the second opening is defined; and a filter member which is disposed inside the cylinder member, and which separates the passage into a first part that includes the first opening, and a second part that includes the second opening.

摘要翻译： 可附接到容纳组织和液体的容器的移液管构件用于通过搅拌从组织中分离细胞，并且包括：气缸构件，其包括容纳在容器中的第一开口，以及 要连接到电池隔离装置的第二开口，其中限定了用于使第一开口与第二开口连通的通道; 以及过滤构件，其设置在所述缸构件的内部，并且将所述通道分离成包括所述第一开口的第一部分，以及包括所述第二开口的第二部分。