公开(公告)号：US20110033880A1

公开(公告)日：2011-02-10

申请号：US12866071

申请日：2007-06-29

申请人： Takako Yada ,  Koji Miyamoto ,  Michinari Honda

发明人： Takako Yada ,  Koji Miyamoto ,  Michinari Honda

IPC分类号： C12Q1/54 ,  C07H21/00 ,  C12N15/63 ,  C12N1/21 ,  C12N1/00 ,  C12N1/15 ,  C12N9/96

CPC分类号： C12N9/0006 ,  C12Q1/006 ,  C12Q1/32 ,  C12Q1/54 ,  C12Y101/9901 ,  G01N27/301 ,  G01N27/302 ,  G01N27/3271 ,  G01N33/66 ,  G01N2333/902 ,  G01N2333/904 ,  Y10T29/49117

摘要： An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.

摘要翻译： 本发明的目的是提供：编码与葡萄糖具有优异的反应性，优异的热稳定性和优异的底物识别性能的优异性质的FAD-共轭葡萄糖脱氢酶的新型基因（多核苷酸），还具有低活性 麦芽糖 使用用该基因转染的转化体细胞来生产酶的方法; 和测定葡萄糖的方法，用于测定葡萄糖的试剂组合物，用于测定葡萄糖的生物传感器等，其特征在于使用所得的酶。 本发明涉及编码FAD-缀合的葡萄糖脱氢酶的多核苷酸，其包含含有氨基酸序列的多肽：X1-X2-X3-X4-X5-X6（其中X1和X2独立地代表脂族氨基酸残基; X3和X6 独立地表示支链氨基酸残基; X4和X5独立地表示杂环氨基酸残基或芳香族氨基酸残基）; 和别的。

公开(公告)号：US08492130B2

公开(公告)日：2013-07-23

申请号：US12866071

申请日：2007-06-29

申请人： Takako Yada ,  Koji Miyamoto ,  Michinari Honda

发明人： Takako Yada ,  Koji Miyamoto ,  Michinari Honda

IPC分类号： C12Q1/54 ,  C12N9/00 ,  C12N9/96 ,  C12N1/20 ,  C12N15/00

CPC分类号： C12N9/0006 ,  C12Q1/006 ,  C12Q1/32 ,  C12Q1/54 ,  C12Y101/9901 ,  G01N27/301 ,  G01N27/302 ,  G01N27/3271 ,  G01N33/66 ,  G01N2333/902 ,  G01N2333/904 ,  Y10T29/49117

摘要： An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.

摘要翻译： 本发明的目的是提供：编码与葡萄糖具有优异的反应性，优异的热稳定性和优异的底物识别性能的优异性质的FAD-共轭葡萄糖脱氢酶的新型基因（多核苷酸），还具有低活性 麦芽糖 使用用该基因转染的转化体细胞来生产酶的方法; 和测定葡萄糖的方法，用于测定葡萄糖的试剂组合物，用于测定葡萄糖的生物传感器等，其特征在于使用所得的酶。 本发明涉及编码FAD-缀合的葡萄糖脱氢酶的多核苷酸，其包含含有氨基酸序列的多肽：X1-X2-X3-X4-X5-X6（其中X1和X2独立地表示脂肪族氨基酸残基; X3和X6 独立地表示支链氨基酸残基; X4和X5独立地表示杂环氨基酸残基或芳香族氨基酸残基）; 和别的。

公开(公告)号：US20120122130A1

公开(公告)日：2012-05-17

申请号：US11886885

申请日：2006-03-27

申请人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Ayaka Atsumi ,  Tetsunari Morita ,  Emi Ishimaru

发明人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Ayaka Atsumi ,  Tetsunari Morita ,  Emi Ishimaru

IPC分类号： C12Q1/54 ,  C12N9/96 ,  C12N5/10 ,  C12N1/21 ,  C12N1/19 ,  C12N1/15 ,  C12N15/53 ,  C12N15/63

CPC分类号： C12Q1/006 ,  C12N9/0004 ,  C12N9/0006 ,  C12Q1/004 ,  C12Q1/32 ,  C12Y101/05 ,  C12Y101/9901 ,  G01N27/3271 ,  G01N2333/904

摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.

摘要翻译： 本发明提供了大规模生产辅酶葡萄糖脱氢酶的成员，其对葡萄糖具有优异的底物识别能力，同时对麦芽糖提供低作用。 本发明涉及编码可溶性辅酶葡萄糖脱氢酶的多核苷酸，其在电子受体存在下催化葡萄糖的氧化并具有5％以下麦芽糖的活性; 由多核苷酸的核苷酸序列编码的多肽; 携带多核苷酸的重组载体; 使用重组载体产生的转化细胞; 一种生产多肽的方法，包括培养转化的细胞并从培养产物中收集连接到FAD以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。

公开(公告)号：US20090176262A1

公开(公告)日：2009-07-09

申请号：US12396724

申请日：2009-03-03

申请人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Tetsunari Morita ,  Mika Kuyama ,  Tokuji Ikeda ,  Kenji Kano ,  Seiya Tsujimura

发明人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Tetsunari Morita ,  Mika Kuyama ,  Tokuji Ikeda ,  Kenji Kano ,  Seiya Tsujimura

IPC分类号： C12Q1/54

CPC分类号： C12N9/0004 ,  C12Q1/32 ,  C12Q1/54

摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

摘要翻译： 本发明提供了一种在电子受体存在下催化葡萄糖氧化反应的微生物衍生的可溶性辅酶结合葡萄糖脱氢酶，其麦芽糖活性低至5％以下， 菲咯啉。 本发明还提供了生产辅酶结合葡萄糖脱氢酶的方法，以及使用辅酶结合葡萄糖脱氢酶进行测定的方法和试剂。 根据本发明，辅酶结合葡萄糖脱氢酶可以应用于工业领域，并且也可以在材料生产或分析中使用，包括使用辅酶结合葡萄糖脱氢酶作为测定或消除样品中的葡萄糖的方法 以及作为有机化合物的制造方法。 还可以提供能够精确测量血糖水平的葡萄糖传感器。 因此，可以提供具有高效用的酶，例如用于改变药物，临床研究和食品领域的材料的能力。

公开(公告)号：US20100297743A1

公开(公告)日：2010-11-25

申请号：US12851668

申请日：2010-08-06

申请人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Tetsunari Morita ,  Mika Kuyama ,  Tokuji Ikeda ,  Kenji Kano ,  Seiya Tsujimura

发明人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Tetsunari Morita ,  Mika Kuyama ,  Tokuji Ikeda ,  Kenji Kano ,  Seiya Tsujimura

IPC分类号： C12M1/34

CPC分类号： C12N9/0004 ,  C12Q1/32 ,  C12Q1/54

摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

摘要翻译： 本发明提供了一种在电子受体存在下催化葡萄糖氧化反应的微生物衍生的可溶性辅酶结合葡萄糖脱氢酶，其麦芽糖活性低至5％以下， 菲咯啉。 本发明还提供了生产辅酶结合葡萄糖脱氢酶的方法，以及使用辅酶结合葡萄糖脱氢酶进行测定的方法和试剂。 根据本发明，辅酶结合葡萄糖脱氢酶可以应用于工业领域，并且也可以在材料生产或分析中使用，包括使用辅酶结合葡萄糖脱氢酶作为测定或消除样品中的葡萄糖的方法 以及作为有机化合物的制造方法。 还可以提供能够精确测量血糖水平的葡萄糖传感器。 因此，可以提供具有高效用的酶，例如用于改变药物，临床研究和食品领域的材料的能力。

公开(公告)号：US20060063217A1

公开(公告)日：2006-03-23

申请号：US10540025

申请日：2003-12-24

申请人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Tetsunari Morita ,  Mika Kuyama ,  Tokuji Ikeda ,  Kenji Kano ,  Seiya Tsujimura

发明人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Tetsunari Morita ,  Mika Kuyama ,  Tokuji Ikeda ,  Kenji Kano ,  Seiya Tsujimura

IPC分类号： C12Q1/54 ,  C12N9/02 ,  C12N1/16

CPC分类号： C12N9/0004 ,  C12Q1/32 ,  C12Q1/54

摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

公开(公告)号：US08691547B2

公开(公告)日：2014-04-08

申请号：US11886885

申请日：2006-03-27

申请人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Ayaka Atsumi ,  Tetsunari Morita ,  Emi Ishimaru

发明人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Ayaka Atsumi ,  Tetsunari Morita ,  Emi Ishimaru

IPC分类号： C12N9/04 ,  C12N9/02 ,  C12N9/96 ,  C12N1/20 ,  C12N1/00 ,  C12N15/00 ,  C12Q1/54 ,  C07H21/04

CPC分类号： C12Q1/006 ,  C12N9/0004 ,  C12N9/0006 ,  C12Q1/004 ,  C12Q1/32 ,  C12Y101/05 ,  C12Y101/9901 ,  G01N27/3271 ,  G01N2333/904

摘要： The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.

摘要翻译： 本发明提供了大规模生产辅酶葡萄糖脱氢酶的成员，其对葡萄糖具有优异的底物识别能力，同时对麦芽糖提供低作用。 本发明涉及编码可溶性辅酶葡萄糖脱氢酶的多核苷酸，其在电子受体存在下催化葡萄糖的氧化并具有5％以下麦芽糖的活性; 由多核苷酸的核苷酸序列编码的多肽; 携带多核苷酸的重组载体; 使用重组载体产生的转化细胞; 一种生产多肽的方法，包括培养转化的细胞并从培养产物中收集连接到FAD以发挥葡萄糖脱水活性的多肽; 使用多肽测定葡萄糖的方法; 用于测定葡萄糖的试剂组合物; 和生物传感器。

公开(公告)号：US07514250B2

公开(公告)日：2009-04-07

申请号：US10540025

申请日：2003-12-24

申请人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Tetsunari Morita ,  Mika Kuyama ,  Tokuji Ikeda ,  Kenji Kano ,  Seiya Tsujimura

发明人： Hironori Omura ,  Hirokazu Sanada ,  Takako Yada ,  Tetsunari Morita ,  Mika Kuyama ,  Tokuji Ikeda ,  Kenji Kano ,  Seiya Tsujimura

IPC分类号： C12N9/02

CPC分类号： C12N9/0004 ,  C12Q1/32 ,  C12Q1/54

摘要： The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level. Therefore, it became possible to provide an enzyme having a high utility, such as an ability of being used for modifying a material in the fields of pharmaceuticals, clinical studies and food products.

摘要翻译： 本发明提供了一种在电子受体存在下催化葡萄糖氧化反应的微生物衍生的可溶性辅酶结合葡萄糖脱氢酶，其麦芽糖活性低至5％以下， 菲咯啉。 本发明还提供了生产辅酶结合葡萄糖脱氢酶的方法，以及使用辅酶结合葡萄糖脱氢酶进行测定的方法和试剂。 根据本发明，辅酶结合葡萄糖脱氢酶可以应用于工业领域，并且也可以在材料生产或分析中使用，包括使用辅酶结合葡萄糖脱氢酶作为测定或消除样品中的葡萄糖的方法 以及作为有机化合物的制造方法。 还可以提供能够精确测量血糖水平的葡萄糖传感器。 因此，可以提供具有高效用的酶，例如用于改变药物，临床研究和食品领域的材料的能力。