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    Method for analyzing gene expression frequency
    2.
    发明授权
    Method for analyzing gene expression frequency 失效
    分析基因表达频率的方法

    公开(公告)号：US06806049B1

    公开(公告)日：2004-10-19

    申请号：US09926028

    申请日：2001-08-16

    申请人： Takami Maekawa ,  Akira Mitsui ,  Masayo Date ,  Hisao Fukuda ,  Yoshiyuki Takahara

    发明人： Takami Maekawa ,  Akira Mitsui ,  Masayo Date ,  Hisao Fukuda ,  Yoshiyuki Takahara

    IPC分类号： C12Q168

    CPC分类号： C12Q1/6809 ,  C12Q2525/191 ,  C12Q2521/313

    摘要： In gene expression frequency analysis, a vector primer to which each cDNA is ligated is formed by synthesizing the cDNA from each mRNA using by a vector primer having a poly(T) sequence; each cDNA sequence is converted to a tag on the vector primer; a concatemer is formed by ligating the obtained tags via a sequence that enables recognition of ends of the tags; and the nucleotide sequence of the concatemer is analyzed.

    摘要翻译： 在基因表达频率分析中，通过使用具有聚（T）序列的载体引物合成来自每个mRNA的cDNA，形成连接有每个cDNA的载体引物; 将每个cDNA序列转化为载体引物上的标签; 通过使得能够识别标签的末端的序列结合所获得的标签来形成并行器; 并对该并发物的核苷酸序列进行分析。

    Method of searching for gene encoding nuclear transport protein
    3.
    发明授权
    Method of searching for gene encoding nuclear transport protein 失效
    搜索编码核转运蛋白的基因的方法

    公开(公告)号：US07195869B2

    公开(公告)日：2007-03-27

    申请号：US10204310

    申请日：2001-11-06

    申请人： Takami Maekawa ,  Maiko Mori ,  Yoshiyuki Takahara

    发明人： Takami Maekawa ,  Maiko Mori ,  Yoshiyuki Takahara

    IPC分类号： C12Q1/68 ,  G01N33/53 ,  C12N15/00 ,  C07K1/00 ,  C07H21/04

    CPC分类号： C12N15/1086 ,  C07K2319/09 ,  C07K2319/60 ,  C12N15/1051 ,  C12N15/1072

    摘要： Each gene in a group comprising many genes is cloned into an expression vector so that its gene product should be expressed as a fusion protein with a protein emitting self-fluorescence to construct a gene expression library. The gene expression library is introduced into cells of two groups to express the fusion proteins in the cells, and only one cell group is stimulated by a drug or the like. Then, cells in which the fusion proteins are localized in the nuclei or their nuclei are isolated from cells of the two groups, respectively. By comparing these cells, genes coding for proteins that are transported into the nuclei specifically in response to a certain stimulus when the stimulus is applied to the cells are retrieved.

    摘要翻译： 将包含许多基因的组中的每个基因克隆到表达载体中，使得其基因产物应当被表达为具有发射自体荧光的蛋白质的融合蛋白以构建基因表达文库。 将基因表达文库导入两组细胞，以在细胞中表达融合蛋白，只有一个细胞组被药物等刺激。 然后，分别从两组的细胞中分离其中融合蛋白定位于细胞核或其核中的细胞。 通过比较这些细胞，检索编码当刺激施加到细胞时特异性地响应于某种刺激而被转运到细胞核中的蛋白质的基因。

    Method for screening a salty taste modulating substance
    4.
    发明授权
    Method for screening a salty taste modulating substance 有权
    筛选咸味调节物质的方法

    公开(公告)号：US09222944B2

    公开(公告)日：2015-12-29

    申请号：US13190885

    申请日：2011-07-26

    申请人： Yutaka Ishiwatari ,  Yuko Kai ,  Takami Maekawa ,  Yuzuru Eto

    发明人： Yutaka Ishiwatari ,  Yuko Kai ,  Takami Maekawa ,  Yuzuru Eto

    IPC分类号： C12Q1/68 ,  G01N33/68 ,  G01N33/50

    CPC分类号： G01N33/6872 ,  G01N33/5008

    摘要： By contacting a test substance with a cell that expresses a Kv3.2 protein, and comparing observed cation influx into the cell with cation influx into the cell observed when the test substance is not contacted with the cell, a salty taste modulating substance such as salt alternative compound, or salt perception enhancing or inhibiting compound is screened.

    摘要翻译： 通过使测试物质与表达Kv3.2蛋白质的细胞接触，并且当测试物质不与细胞接触时观察到的阳离子进入细胞，将观察到的阳离子流入细胞与阳离子进入比较，将咸味调节物质如盐 替代化合物或盐感知增强或抑制化合物被筛选。

    Glutamate receptor and utilization thereof
    5.
    发明授权
    Glutamate receptor and utilization thereof 有权
    谷氨酸受体及其利用

    公开(公告)号：US07186819B2

    公开(公告)日：2007-03-06

    申请号：US10918857

    申请日：2004-12-10

    申请人： Ana San Gabriel ,  Hisayuki Uneyama ,  Takami Maekawa ,  Kunio Torii

    发明人： Ana San Gabriel ,  Hisayuki Uneyama ,  Takami Maekawa ,  Kunio Torii

    IPC分类号： C12N15/12

    CPC分类号： C07K14/70571

    摘要： The inventive subject matter relates to a glutamate receptor, DNA which encodes the receptor, a transformed cell expressing the receptor, a method for producing the receptor, a method for identifying an agonist, antagonist, or allosteric modulator for glutamic acid, a method for identifying an agonist for glutamic acid, an antibody to the receptor, and processes for making glutamate receptor modulators and pharmaceutical compositions comprising said modulator.

    摘要翻译： 本发明涉及谷氨酸受体，编码受体的DNA，表达受体的转化细胞，产生受体的方法，用于鉴定谷氨酸的激动剂，拮抗剂或变构调节剂的方法，鉴定方法 谷氨酸的激动剂，受体的抗体，以及制备谷氨酸受体调节剂的方法和包含所述调节剂的药物组合物。

    Glutamic acid receptor and utilization thereof
    8.
    发明授权
    Glutamic acid receptor and utilization thereof 有权
    谷氨酸受体及其利用

    公开(公告)号：US07157250B2

    公开(公告)日：2007-01-02

    申请号：US10828332

    申请日：2004-04-21

    申请人： Ana San Gabriel ,  Takami Maekawa ,  Hisayuki Uneyama ,  Kunio Torii

    发明人： Ana San Gabriel ,  Takami Maekawa ,  Hisayuki Uneyama ,  Kunio Torii

    IPC分类号： C12P21/06 ,  C07K5/00

    CPC分类号： C07K14/70571 ,  A61K38/00 ,  G01N2500/00

    摘要： An agonist or antagonist of glutamic acid or an allosteric modulator is screened by reacting a glutamic acid receptor protein having the following properties with a substance binding thereto in the presence of a test substance and detecting the inhibition or promotion of the reaction:(A) it has a transmembrane domain and an intracellular domain common to those of type 4 metabotropic glutamic acid receptor protein, or(B) it has an extracellular domain by about 316 or 327 amino acid residues shorter than that of the type 4 metabotropic glutamic acid receptor protein.

    摘要翻译： 谷氨酸或变构调节剂的激动剂或拮抗剂通过使具有以下特性的谷氨酸受体蛋白质与与其结合的物质在测试物质存在下进行筛选，并检测反应的抑制或促进作用：（A） 具有与4型代谢型谷氨酸受体蛋白质共同的跨膜结构域和细胞内结构域，或（B）其具有比4型代谢型谷氨酸受体蛋白质短约316或327个氨基酸残基的细胞外结构域。

    Novel glutamate receptors and utilization thereof
    9.
    发明申请
    Novel glutamate receptors and utilization thereof 有权
    新型谷氨酸受体及其利用

    公开(公告)号：US20060040350A1

    公开(公告)日：2006-02-23

    申请号：US10922166

    申请日：2004-08-20

    申请人： Ana San Gabriel ,  Hisayuki Uneyama ,  Takami Maekawa ,  Kunio Torii

    发明人： Ana San Gabriel ,  Hisayuki Uneyama ,  Takami Maekawa ,  Kunio Torii

    IPC分类号： C07H21/04 ,  C12P21/06 ,  C07K14/705

    CPC分类号： C07K14/70571

    摘要： The inventive subject matter relates to novel glutamate receptors and utilization thereof; more specifically to a glutamate receptor, DNA which encodes the receptor, a transformed cell expressing the receptor, a method for producing the receptor, a method for identifying an agonist, antagonist, or allosteric modulator for glutamic acid, a method for identifying an agonist for glutamic acid, an antibody to the receptor, and processes for making glutamate receptor modulators and pharmaceutical compositions comprising said modulator.

    摘要翻译： 本发明涉及新型谷氨酸受体及其利用; 更具体地涉及谷氨酸受体，编码受体的DNA，表达受体的转化细胞，产生受体的方法，用于鉴定谷氨酸的激动剂，拮抗剂或变构调节剂的方法，鉴定用于谷氨酸的激动剂的方法 谷氨酸，对受体的抗体，以及制备谷氨酸受体调节剂的方法和包含所述调节剂的药物组合物。

    Novel glutamate receptor and utilization thereof
    10.
    发明申请
    Novel glutamate receptor and utilization thereof 有权
    新型谷氨酸受体及其利用

    公开(公告)号：US20050282746A1

    公开(公告)日：2005-12-22

    申请号：US10918857

    申请日：2004-12-10

    申请人： Ana Gabriel ,  Hisayuki Uneyama ,  Takami Maekawa ,  Kunio Torii

    发明人： Ana Gabriel ,  Hisayuki Uneyama ,  Takami Maekawa ,  Kunio Torii

    IPC分类号： A61P1/04 ,  A61P1/12 ,  A61P1/14 ,  A61P3/04 ,  A61P3/10 ,  A61P43/00 ,  C07K14/705 ,  C12N15/12 ,  A61K38/17 ,  C07H21/04

    CPC分类号： C07K14/70571

    摘要： It is intended to disclose a glutamate sensor in taste sensation and digestion and provide techniques using the same. A glutamate-receptor protein having the following characteristics is reacted with a substance binding thereto in the presence of a test substance and thus the inhibition or acceleration of the reaction is detected to thereby search for a glutamate agonist or antagonist or an allosteric modulator: (1) having a transmembrane domain and an intracellular domain common to a type 1 metabolic glutamate receptor protein; and (2) having an extracellular domain shorter by about 409 or 481 amino acid residues than the type 1 metabolic glutamate receptor protein.

    摘要翻译： 旨在公开味觉和消化中的谷氨酸盐传感器，并提供使用其的技术。 具有以下特征的谷氨酸受体蛋白质在与测试物质的存在下与结合的物质反应，因此检测到反应的抑制或加速，从而搜索谷氨酸激动剂或拮抗剂或变构调节剂：（1 ）具有跨膜结构域和1型代谢谷氨酸受体蛋白共有的细胞内结构域; 和（2）具有比1型代谢谷氨酸受体蛋白质更短约409或481个氨基酸残基的细胞外结构域。