公开(公告)号：US4145253A

公开(公告)日：1979-03-20

申请号：US845970

申请日：1977-10-27

申请人： Takao Iida ,  Kunikatsu Shirahata ,  Isao Maisubara ,  Masahiro Sugimoto ,  Shinzo Ishii ,  Ryo Okachi ,  Takashi Nara

发明人： Takao Iida ,  Kunikatsu Shirahata ,  Isao Maisubara ,  Masahiro Sugimoto ,  Shinzo Ishii ,  Ryo Okachi ,  Takashi Nara

IPC分类号： C07H15/224 ,  C12D9/14

CPC分类号： C07H15/224 ,  Y10S435/863

摘要： New antibiotic compounds, Fortimicin factors D and KE are produced by fermentation of microorganisms belonging to the genus Micromonospora. The antibiotic compounds are accumulated in the culture liquor and are isolated therefrom. A semisynthetic method of producing Fortimicin KE utilizing Fortimicin D is also disclosed.

摘要翻译： 新型抗生素化合物，Fortimicin因子D和KE通过发酵属于小单孢菌属的微生物产生。 抗生素化合物在培养液中积聚并从中分离。 还公开了使用Fortimicin D生产Fortimicin KE的半合成方法。

公开(公告)号：US4209612A

公开(公告)日：1980-06-24

申请号：US957845

申请日：1978-11-06

申请人： Keiichi Takahashi ,  Takao Iida ,  Kunikatsu Shirahata ,  Masahiro Sugimoto ,  Shinzo Ishii ,  Ryo Okachi ,  Takashi Nara

发明人： Keiichi Takahashi ,  Takao Iida ,  Kunikatsu Shirahata ,  Masahiro Sugimoto ,  Shinzo Ishii ,  Ryo Okachi ,  Takashi Nara

IPC分类号： A61K31/35 ,  A61K31/351 ,  A61P31/04 ,  C07D309/28 ,  C07H15/224 ,  C12P19/48 ,  C07H15/22

CPC分类号： C07H15/224 ,  C12P19/48

摘要： New antibiotic compounds, Fortimicin factors KF and KG are produced by fermentation of microorganisms belonging to the genus Micromonospora. The antibiotic compounds are accumulated in the culture liquor and are isolated therefrom.

摘要翻译： 新型抗生素化合物，Fortimicin因子KF和KG通过发酵属于小单孢菌属的微生物产生。 抗生素化合物在培养液中积聚并从中分离。

公开(公告)号：US4216308A

公开(公告)日：1980-08-05

申请号：US974434

申请日：1978-12-29

申请人： Takao Iida ,  Kunikatsu Shirahata ,  Isao Matsubara ,  Masahiro Sugimoto ,  Shinzo Ishii ,  Ryo Okachi ,  Takashi Nara

发明人： Takao Iida ,  Kunikatsu Shirahata ,  Isao Matsubara ,  Masahiro Sugimoto ,  Shinzo Ishii ,  Ryo Okachi ,  Takashi Nara

IPC分类号： C07H15/224 ,  A61K31/71 ,  C07H15/22

CPC分类号： C07H15/224 ,  Y10S435/863

摘要： New antibiotic compounds, Fortimicin factors D and KE are produced by fermentation of microorganisms belonging to the genus Micromonospora. The antibiotic compounds are accumulated in the culture liquor and are isolated therefrom. A semisynthetic method of producing Fortimicin KE utilizing Fortimicin D is also disclosed.

公开(公告)号：US4310661A

公开(公告)日：1982-01-12

申请号：US219586

申请日：1980-12-23

申请人： Takao Iida ,  Kunikatsu Shirahata ,  Shinzo Ishii ,  Ryo Okachi ,  Takashi Nara

发明人： Takao Iida ,  Kunikatsu Shirahata ,  Shinzo Ishii ,  Ryo Okachi ,  Takashi Nara

IPC分类号： C07H15/236 ,  C07H15/22 ,  A61K31/71

CPC分类号： C07H15/236 ,  Y10S435/867

摘要： New antibacterial compounds, XK-62-3 and XK-62-4, are produced by fermentation of microorganisms belonging to the genus Micromonospora. The compounds are accumulated in the culture liquor and are isolated therefrom.

摘要翻译： 新型抗菌化合物XK-62-3和XK-62-4是通过发酵属于小单孢菌属的微生物生产的。 将化合物积聚在培养液中并与其分离。

公开(公告)号：US4298690A

公开(公告)日：1981-11-03

申请号：US906333

申请日：1978-05-16

申请人： Takao Iida ,  Kunikatsu Shirahata ,  Shinzo Ishii ,  Ryo Okachi ,  Takashi Nara

发明人： Takao Iida ,  Kunikatsu Shirahata ,  Shinzo Ishii ,  Ryo Okachi ,  Takashi Nara

IPC分类号： C07H15/236 ,  C12P19/48 ,  C12N1/20 ,  C12R1/29

CPC分类号： C07H15/236 ,  Y10S435/867

摘要： New antibacterial compounds, XK-62-3 and XK-62-4, are produced by fermentation of microorganisms belonging to the genus Micromonospora. The compounds are accumulated in the culture liquor and are isolated therefrom.

摘要翻译： 新型抗菌化合物XK-62-3和XK-62-4是通过发酵属于小单孢菌属的微生物生产的。 将化合物积聚在培养液中并与其分离。

公开(公告)号：US4296040A

公开(公告)日：1981-10-20

申请号：US97001

申请日：1970-11-23

申请人： Satoshi Omura ,  Haruo Tanaka ,  Itaru Takahashi ,  Shinzo Ishii ,  Kazuyuki Mineura ,  Kunikatsu Shirahata ,  Masaji Kasai

发明人： Satoshi Omura ,  Haruo Tanaka ,  Itaru Takahashi ,  Shinzo Ishii ,  Kazuyuki Mineura ,  Kunikatsu Shirahata ,  Masaji Kasai

IPC分类号： A61K31/35 ,  A61P31/04 ,  C07D493/08 ,  C12P17/18 ,  C12R1/465 ,  C07D311/78

CPC分类号： C07D493/08 ,  C12P17/181 ,  Y10S435/886

摘要： The present invention teaches a new antibiotic nanaomycin E represented by the formula: ##STR1## Nanaomycin E is active against Gram-positive bacteria, Trichophyton and Mycoplasma and may be used as antibacterial and therapeutic agents for humans and animals. Nanaomycin E is also a useful starting maerial for the preparation of nanaomycin A which latter nanaomycin has the highest activity among the various nanaomycin-type compounds. Nanaomycin E is produced by fermentation of a microorganism belonging to the genus Streptomyces and capable of producing nanaomycin E, especially Streptomyces rosa variant notoensis (FERM-P 2209; ATCC 31135) and recovering the same from the fermented liquor.

摘要翻译： 本发明教导了由下式表示的新型抗生素纳那霉素E：Nanaomycin E对革兰氏阳性菌，毛癣菌和支原体具有活性，可用作人和动物的抗菌剂和治疗剂。 纳豆霉素E也是制备纳诺霉素A的有用起始物，后者纳那霉素在各种纳诺霉素型化合物中活性最高。 纳豆霉素E是通过发酵属于链霉菌属的微生物产生的，并且能够产生纳诺霉素E，特别是罗氏变形链霉菌（FERM-P 2209; ATCC 31135），并从发酵液中回收。

公开(公告)号：US4353986A

公开(公告)日：1982-10-12

申请号：US276432

申请日：1981-06-22

申请人： Satoshi Omura ,  Haruo Tanaka ,  Itaru Takahashi ,  Shinzo Ishii ,  Kazuyuki Mineura ,  Kunikatsu Shirahata ,  Masaji Kasai

发明人： Satoshi Omura ,  Haruo Tanaka ,  Itaru Takahashi ,  Shinzo Ishii ,  Kazuyuki Mineura ,  Kunikatsu Shirahata ,  Masaji Kasai

IPC分类号： A61K31/35 ,  A61P31/04 ,  C07D493/08 ,  C12P17/18 ,  C12R1/465 ,  C12P17/06

CPC分类号： C07D493/08 ,  C12P17/181 ,  Y10S435/886

摘要： The present invention teaches a new antibiotic nanaomycin E represented by the formula: ##STR1## Nanaomycin E is active against Gram-positive bacteria, Trichophyton and Mycoplasma and may be used as antibacterial and therapeutic agents for humans and animals. Nanaomycin E is also a useful starting material for the preparation of nanaomycin A which latter nanaomycin has the highest activity among the various nanaomycin-type compounds. Nanaomycin E is produced by fermentation of a microorganism belonging to the genus Streptomyces and capable of producing nanaomycin E, especially Streptomyces rosa variant notoensis (FERM-P 2209; ATCC 31135) and recovering the same from the fermented liquor.

摘要翻译： 本发明教导了由下式表示的新型抗生素纳那霉素E：Nanaomycin E对革兰氏阳性菌，毛癣菌和支原体具有活性，可用作人和动物的抗菌剂和治疗剂。 纳那霉素E也是制备纳诺霉素A的有用起始原料，后者纳那霉素在各种纳诺霉素型化合物中具有最高的活性。 纳豆霉素E是通过发酵属于链霉菌属的微生物产生的，并且能够产生纳诺霉素E，特别是罗氏不动杆菌（FERM-P 2209; ATCC 31135），并从发酵液中回收。

公开(公告)号：US4563462A

公开(公告)日：1986-01-07

申请号：US546142

申请日：1983-10-27

申请人： Shinzo Ishii ,  Shigeo Katsumata ,  Yuko Arai ,  Tadashi Ashizawa ,  Makoto Morimoto ,  Kunikatsu Shirahata ,  Yutaka Saito ,  Motomichi Kono

发明人： Shinzo Ishii ,  Shigeo Katsumata ,  Yuko Arai ,  Tadashi Ashizawa ,  Makoto Morimoto ,  Kunikatsu Shirahata ,  Yutaka Saito ,  Motomichi Kono

IPC分类号： A61K31/47 ,  A61P35/00 ,  A61P35/02 ,  C07D471/16 ,  C12P17/18 ,  C12R1/465

CPC分类号： C07D471/16 ,  C12P17/182 ,  C12R1/465

摘要： A substance designated by us as AX-2 and represented by the formula: ##STR1## This substance is produced by culturing a microorganism of the genus Streptomyces and capable of producing AX-2 in a medium to accumulate AX-2 in the cultured broth and isolating AX-2 therefrom.An anti-tumor composition comprising an effective amount of AX-2 in association with a physiologically acceptable carrier or excipient, which is active against Sarcoma 180.

摘要翻译： 由我们指定为AX-2并由下式表示的物质：该物质通过培养链霉菌属的微生物并能够在培养基中产生AX-2并在培养的肉汤中积累AX-2而产生AX-2， 从其中分离AX-2。 一种抗肿瘤组合物，其包含与生理上可接受的载体或赋形剂相关联的有效量的AX-2，其对肉瘤180有活性。

公开(公告)号：US4649199A

公开(公告)日：1987-03-10

申请号：US697918

申请日：1985-02-04

申请人： Shinzo Ishii ,  Shigeo Katsumata ,  Yukou Arai ,  Kazuhisa Fujimoto ,  Makoto Morimoto

发明人： Shinzo Ishii ,  Shigeo Katsumata ,  Yukou Arai ,  Kazuhisa Fujimoto ,  Makoto Morimoto

IPC分类号： C07D498/22 ,  C12P17/18 ,  C12R1/465 ,  A61K31/495

CPC分类号： C07D498/22

摘要： A pharmacologically acceptable acid addition salt of DC-52 with 0.5-2.0 equivalent weight of inorganic acid, sulfonic acid, acidic amino acid, citric acid, transaconitic acid, .alpha.-ketoglutaric acid, itaconic acid, malonic acid or ascorbic acid on the basis of DC-52, having the same degree of antitumor activity as DC-52, is superior to DC-52 in stability at powdering or at preservation.

摘要翻译： 磺酸，酸性氨基酸，柠檬酸，经官能度酸，α-酮戊二酸，衣康酸，丙二酸或抗坏血酸的基础上，加入0.5〜2.0当量的DC-52的药理学上可接受的酸加成盐 与DC-52具有相同程度的抗肿瘤活性的DC-52在粉化或保存时的稳定性优于DC-52。

公开(公告)号：US4560661A

公开(公告)日：1985-12-24

申请号：US488174

申请日：1983-04-25

申请人： Hideo Katsumata ,  Shigeo Katsumata ,  Shinzo Ishii ,  Yuko Arai

发明人： Hideo Katsumata ,  Shigeo Katsumata ,  Shinzo Ishii ,  Yuko Arai

IPC分类号： C12N9/00 ,  C12N9/04 ,  C12N9/18 ,  C12N9/52 ,  C12N9/80 ,  C12N9/02 ,  C12N9/14 ,  C12N9/20 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92

CPC分类号： C12N9/0006 ,  C12N9/00 ,  C12N9/18 ,  C12N9/52 ,  C12N9/80 ,  Y10S435/815

摘要： Disclosed is a process for purifying an enzyme contained in a solution such as cell extract liquor or fermentation culture liquor. The crude enzyme solution is brought into contact with either a strongly acidic cation exchange resin of high porous type or a strongly basic anion exchange resin of high porous type to adsorb the enzyme on the resin. An eluting agent is then passed through the resin to elute out the enzyme as a purified enzyme solution.

摘要翻译： 公开了一种纯化细胞提取液或发酵培养液等溶液中所含的酶的方法。 将粗酶溶液与高多孔型的强酸性阳离子交换树脂或高多孔型的强碱性阴离子交换树脂接触以将酶吸附在树脂上。 然后将洗脱剂通过树脂以作为纯化酶溶液洗脱出酶。