公开(公告)号：US20100141752A1

公开(公告)日：2010-06-10

申请号：US12629547

申请日：2009-12-02

申请人： Tatsuki Yamada ,  Shinsuke Tani ,  Takeshi Otsuka ,  Satoshi Arai ,  Yuichi Ishikawa ,  Kengo Takeuchi

发明人： Tatsuki Yamada ,  Shinsuke Tani ,  Takeshi Otsuka ,  Satoshi Arai ,  Yuichi Ishikawa ,  Kengo Takeuchi

IPC分类号： G09G5/02 ,  H04N7/18 ,  G09G5/00

CPC分类号： G01N1/312 ,  G01N2035/00039 ,  G01N2035/00138 ,  G02B21/26 ,  G02B21/367

摘要： A microscope system includes an image acquiring unit that acquires a specimen image formed by capturing a specimen multi-stained by a plurality of pigments using a microscope; a pigment amount acquiring unit that acquires a pigment amount of each pigment staining a corresponding position on the specimen, for each pixel of the specimen image; and a pigment selecting unit that selects a display target pigment from the plurality of pigments. The system also includes a display image generating unit that generates a display image where a staining state of the specimen by the display target pigment is displayed, on the basis of the pigment amount of the display target pigment in each pixel of the specimen image; and a display processing unit that displays the display image on a display unit.

摘要翻译： 显微镜系统包括图像获取单元，其获取通过使用显微镜捕获多个颜料多染色的样本而形成的标本图像; 颜料量获取单元，针对样本图像的每个像素获取每个颜料的颜料量染色样品上的相应位置; 以及颜料选择单元，其从多种颜料中选择显示目标颜料。 该系统还包括显示图像生成单元，其基于样本图像的每个像素中的显示目标颜料的颜料量，生成显示目标颜料的样品的染色状态的显示图像; 以及显示处理单元，其将显示图像显示在显示单元上。

公开(公告)号：US09110305B2

公开(公告)日：2015-08-18

申请号：US12950268

申请日：2010-11-19

申请人： Tatsuki Yamada ,  Yuichi Ishikawa ,  Kengo Takeuchi

发明人： Tatsuki Yamada ,  Yuichi Ishikawa ,  Kengo Takeuchi

IPC分类号： H04N7/18 ,  G02B21/36 ,  G01N1/30

CPC分类号： G02B21/365 ,  G01N1/30

摘要： A microscope system includes an acquisition unit that obtains a specimen image acquired by capturing the specimen stained by an element identification dye that visualizes a predetermined cell constituent element and a molecule target dye that visualizes a predetermined target molecule by using a microscope; a dye amount unit that obtains dye amounts of the element identification dye and the molecule target dye that stain corresponding positions on the specimen for each pixel of the image; an element area identification unit that identifies an area of the cell constituent element based on the dye amount of the element identification dye; a condition setting unit that sets the presence or absence of the predetermined target molecule on the cell constituent element as a condition; and an extraction unit that extracts an area of a target portion that satisfies the condition based on the dye amount of the molecule target dye.

摘要翻译： 显微镜系统包括获取单元，其获取通过捕获通过使用显微镜可视化预定细胞构成元素的元素识别染料和可视化预定靶分子的分子靶染料而染色的样品而获得的标本图像; 染色量单元，其获得染色剂量，所述染料量对于图像的每个像素染色样品上的相应位置的元素识别染料和分子靶染料; 元件区域识别单元，其基于元件识别染料的染色量来识别单元构成元件的面积; 条件设定单元，其将所述细胞构成元件上的所述规定的目标分子的存在或不存在作为条件; 以及提取单元，其基于分子靶染料的染料量提取满足条件的目标部分的面积。

公开(公告)号：US20110212486A1

公开(公告)日：2011-09-01

申请号：US12950268

申请日：2010-11-19

申请人： Tatsuki Yamada ,  Yuichi Ishikawa ,  Kengo Takeuchi

发明人： Tatsuki Yamada ,  Yuichi Ishikawa ,  Kengo Takeuchi

IPC分类号： G01N33/48 ,  C12M1/34

CPC分类号： G02B21/365 ,  G01N1/30

摘要： A microscope system includes an acquisition unit that obtains a specimen image acquired by capturing the specimen stained by an element identification dye that visualizes a predetermined cell constituent element and a molecule target dye that visualizes a predetermined target molecule by using a microscope; a dye amount unit that obtains dye amounts of the element identification dye and the molecule target dye that stain corresponding positions on the specimen for each pixel of the image; an element area identification unit that identifies an area of the cell constituent element based on the dye amount of the element identification dye; a condition setting unit that sets the presence or absence of the predetermined target molecule on the cell constituent element as a condition; and an extraction unit that extracts an area of a target portion that satisfies the condition based on the dye amount of the molecule target dye.

摘要翻译： 显微镜系统包括获取单元，其获取通过捕获通过使用显微镜可视化预定细胞构成元素的元素识别染料和可视化预定靶分子的分子靶染料而染色的样品而获得的标本图像; 染色量单元，其获得染色剂量，所述染料量针对图像的每个像素染色样品上的相应位置的元件识别染料和分子靶染料; 元件区域识别单元，其基于元件识别染料的染色量来识别单元构成元件的面积; 条件设定单元，其将所述细胞构成元件上的所述规定的目标分子的存在或不存在作为条件; 以及提取单元，其基于分子靶染料的染料量提取满足条件的目标部分的面积。

公开(公告)号：US20100272334A1

公开(公告)日：2010-10-28

申请号：US12817451

申请日：2010-06-17

申请人： Tatsuki Yamada ,  Satoshi Arai ,  Yoko Yamamoto ,  Yuichi Ishikawa ,  Kengo Takeuchi

发明人： Tatsuki Yamada ,  Satoshi Arai ,  Yoko Yamamoto ,  Yuichi Ishikawa ,  Kengo Takeuchi

IPC分类号： G06T7/00

CPC分类号： G01N1/312 ,  G01N2035/00039 ,  G01N2035/00138 ,  G02B21/26 ,  G02B21/367

摘要： A microscope system includes an image acquisition unit for acquiring a spectral image of a specimen by using a microscope, a structure specifying unit for specifying an extraction target structure in the specimen, a display method specifying unit for specifying a display method of the extraction target structure, a structure extraction unit for extracting an area of the extraction target structure in the spectral image by using a reference spectrum of the extraction target structure on the basis of pixel values of each pixel included in the spectral image, a display image generator for generating a display image that represents the extraction target structure in the specimen by the display method specified by the display method specifying unit on the basis of an extraction result of the structure extraction unit, and a display processing unit for performing process for displaying the display image on a display unit.

摘要翻译： 显微镜系统包括用于通过使用显微镜获取样本的光谱图像的图像获取单元，用于指定样本中的提取目标结构的结构指定单元，用于指定提取目标结构的显示方法的显示方法确定单元 一种结构提取单元，用于根据包括在所述分光图像中的每个像素的像素值，通过使用所述提取目标结构的参考光谱来提取所述光谱图像中的所述提取目标结构的区域;显示图像生成器， 显示图像，其通过基于结构提取单元的提取结果的显示方法指定单元指定的显示方法来显示样本中的提取目标结构;以及显示处理单元，用于执行将显示图像显示在 显示单元。

公开(公告)号：US06259080B1

公开(公告)日：2001-07-10

申请号：US09268587

申请日：1999-03-15

申请人： Zheng Li ,  Takashi Yoneyama ,  Tatsuki Yamada ,  Takashi Nagano

发明人： Zheng Li ,  Takashi Yoneyama ,  Tatsuki Yamada ,  Takashi Nagano

IPC分类号： G02B704

CPC分类号： G02B21/244

摘要： The present invention is an automatic focal point detection device. In a broad visual field and high resolution observation, when focusing is impossible in a splitting visual field obtained by splitting a specimen in a visual field in plural number, a stage is moved to an in-focus position stored in advance and focusing is executed. Besides the device is provided with an excitation light shutter for intercepting an excitation light with which a specimen is irradiated and when stray light corresponding to a difference detected by closing and opening the shutter in fluorescence observation is smaller than a specific value, specimen search is performed and focusing is executed at a level exceeding stray light, while the difference is equal to or larger than the specific value, focusing is executed with nothing more done.

摘要翻译： 本发明是一种自动焦点检测装置。 在宽视野和高分辨率观察中，当通过在多个视野中分割样本而获得的分割视野中不可能进行聚焦时，将舞台移动到预先存储的对焦位置并进行聚焦。 此外，该装置设置有用于截取照射样本的激发光的激发光快门，并且当在荧光观察中对应于通过关闭和打开快门检测到的差异的杂散光小于特定值时，进行样本搜索 并且在超过杂散光的水平上执行聚焦，而差异等于或大于特定值，则不再进行聚焦。

公开(公告)号：US20120033064A1

公开(公告)日：2012-02-09

申请号：US13205922

申请日：2011-08-09

申请人： Tatsuki Yamada ,  Futoshi Akiyama ,  Rie Horii

发明人： Tatsuki Yamada ,  Futoshi Akiyama ,  Rie Horii

IPC分类号： H04N7/18

CPC分类号： H04N7/183 ,  G02B21/0004 ,  G02B21/086 ,  G02B21/26 ,  G02B21/367 ,  G02B26/008

摘要： A microscope system includes a pigment amount calculating unit, a cell component identification processing unit, a target molecule expression portion extraction unit, a cell variant setting unit, a cell variant classification determining unit, and a display image generating unit. The pigment amount calculating unit calculates a pigment amount of a molecule target pigment for each pixel of a VS image. The cell component identification processing unit identifies a cell component based on the pigment amount. The target molecule expression portion extraction unit extracts expression portions of target molecules in the area of the cell component. The cell variant setting unit sets a cell variant including a combination of presence/absence of expressions of target molecules. The cell variant classification determining unit classifies a cell area in the VS image into a cell variant based on the combination of the expression portions of the target molecules included in the cell area.

摘要翻译： 显微镜系统包括颜料量计算单元，单元部件识别处理单元，目标分子表达部分提取单元，单元变体设置单元，单元变体分类确定单元和显示图像生成单元。 颜料量计算单元计算VS图像的每个像素的分子靶颜料的颜料量。 细胞成分识别处理单元基于颜料量鉴定细胞成分。 靶分子表达部分提取单元提取细胞成分区域中的靶分子的表达部分。 细胞变体设置单元设置包括目标分子表达的存在/不存在的组合的细胞变体。 细胞变体分类确定单元基于细胞区域中包含的靶分子的表达部分的组合，将VS图像中的细胞区域分类为细胞变体。

公开(公告)号：US07932504B2

公开(公告)日：2011-04-26

申请号：US12343748

申请日：2008-12-24

申请人： Tatsuki Yamada

发明人： Tatsuki Yamada

IPC分类号： G01N21/64

CPC分类号： G02B21/16 ,  G01N21/6458 ,  G02B21/18

摘要： A whole specimen image generated in the bright view field observation method is displayed, and arbitrary domains are designated. And the fluorescence VS image of each object of interest is generated by acquiring the shape of microscopic image for every small block which is formed by dividing the inside of that object of interest into plurality for every said object of interest, and combining two or more microscopic images generated in the fluorescence observation method. The object of interest is an area where a tumor etc., exist, for example.

摘要翻译： 显示在亮视野观察方法中产生的整体标本图像，并指定任意域。 并且通过对于每个所述感兴趣对象将所述感兴趣对象的内部分成多个而形成的每个小块获取显微图像的形状，并且组合两个或更多个微观的 在荧光观察方法中产生的图像。 感兴趣的对象是例如存在肿瘤等的区域。

公开(公告)号：US20090213214A1

公开(公告)日：2009-08-27

申请号：US12357059

申请日：2009-01-21

申请人： Tatsuki Yamada

发明人： Tatsuki Yamada

IPC分类号： H04N7/18

CPC分类号： G01N21/6458 ,  G02B21/367 ,  G06K9/0014 ,  G06T5/50 ,  G06T7/0012 ,  G06T2200/04 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/10148 ,  G06T2207/30024

摘要： A microscope system has a VS image generation means for generating a virtual slide image of a specimen which is constructed by mutually connecting a plurality of microscope images with a first photomagnification photographed and acquired whenever an objective lens and the specimen are relatively moved in a direction perpendicular to the optical axis and which represents the entire image of the specimen, an object-of-interest set means setting an object of interest with respect to the entire image of the specimen represented by the VS image, and a three-dimensional VS image generation means for generating a three-dimensional VS image which is constructed by connecting the microscope images at different focal positions in accordance with the same focal position and which is constructed from the microscope images with a second photomagnification higher than the first photomagnification and represents the image of the object of interest.

摘要翻译： 显微镜系统具有用于产生样本的虚拟幻灯片图像的VS图像产生装置，每当物镜和样本在垂直方向上相对移动时，通过相互连接多个显微镜图像和第一次光学曝光而获得的样本 相对于由VS图像表示的样本的整个图像设置感兴趣对象的三维VS图像生成装置，其特征在于， 用于产生三维VS图像的装置，其通过根据相同焦点位置连接不同焦点位置处的显微镜图像而构成，并且由具有高于第一光放大的第二光放大的显微镜图像构成，并且表示 感兴趣的对象。

公开(公告)号：US07250963B2

公开(公告)日：2007-07-31

申请号：US10320132

申请日：2002-12-16

申请人： Kiyoshi Yuri ,  Shuji Nakagawa ,  Tatsuki Yamada

发明人： Kiyoshi Yuri ,  Shuji Nakagawa ,  Tatsuki Yamada

IPC分类号： H04N7/18 ,  H04N9/47

CPC分类号： G02B21/365

摘要： The microscopic image capture apparatus includes: a slide glass transfer unit 17 transfers a slide glass 10 from a first slide glass storage unit 16 to a space under a microscope objective lens 11; a wide field-of-view image capture unit 60 captures the entire image of wide field-of-view of the slide glass 10 in synchronization with the transfer of the slide glass 10 by the slide glass transfer unit 17; a microscopic image capture unit 14 captures a microscopic image of the sample S on the slide glass 10 whose entire image of wide field-of-view has been captured by the wide field-of-view image capture unit 60; and a slide glass storage unit 18 stores the slide glass 10 whose sample S has been captured by the microscopic image capture unit 14 from the observation position of the microscope 11 into the second slide glass tray unit 19.

摘要翻译： 显微图像捕获装置包括：载玻片转移单元17将载玻片10从第一载玻片存储单元16传送到显微镜物镜11下方的空间; 宽视野图像拍摄单元60与滑动玻璃转印单元17的载玻片10的转印同步地捕获载玻片10的整个视场的整个图像; 微观图像捕获单元14捕获由宽视野图像捕获单元60捕获其整个视野的整个图像的载玻片10上的样本S的显微镜图像; 并且载玻片存储单元18将其显微镜图像捕获单元14捕获的样品S的载玻片10从显微镜11的观察位置存储到第二载玻片托盘单元19中。

公开(公告)号：US08937653B2

公开(公告)日：2015-01-20

申请号：US13205922

申请日：2011-08-09

申请人： Tatsuki Yamada ,  Futoshi Akiyama ,  Rie Horii

发明人： Tatsuki Yamada ,  Futoshi Akiyama ,  Rie Horii

IPC分类号： H04N7/18 ,  G02B21/00 ,  G02B21/36 ,  G02B26/00 ,  G02B21/08 ,  G02B21/26

CPC分类号： H04N7/183 ,  G02B21/0004 ,  G02B21/086 ,  G02B21/26 ,  G02B21/367 ,  G02B26/008

摘要： A microscope system includes a pigment amount calculating unit, a cell component identification processing unit, a target molecule expression portion extraction unit, a cell variant setting unit, a cell variant classification determining unit, and a display image generating unit. The pigment amount calculating unit calculates a pigment amount of a molecule target pigment for each pixel of a VS image. The cell component identification processing unit identifies a cell component based on the pigment amount. The target molecule expression portion extraction unit extracts expression portions of target molecules in the area of the cell component. The cell variant setting unit sets a cell variant including a combination of presence/absence of expressions of target molecules. The cell variant classification determining unit classifies a cell area in the VS image into a cell variant based on the combination of the expression portions of the target molecules included in the cell area.

摘要翻译： 显微镜系统包括颜料量计算单元，单元部件识别处理单元，目标分子表达部分提取单元，单元变体设置单元，单元变体分类确定单元和显示图像生成单元。 颜料量计算单元计算VS图像的每个像素的分子靶颜料的颜料量。 细胞成分识别处理单元基于颜料量鉴定细胞成分。 靶分子表达部分提取单元提取细胞成分区域中的靶分子的表达部分。 细胞变体设置单元设置包括目标分子表达的存在/不存在的组合的细胞变体。 细胞变体分类确定单元基于细胞区域中包含的靶分子的表达部分的组合，将VS图像中的细胞区域分类为细胞变体。