公开(公告)号：US08426674B2

公开(公告)日：2013-04-23

申请号：US12521701

申请日：2007-12-28

申请人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

发明人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

IPC分类号： A01K67/04

CPC分类号： C12N9/16

摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.

摘要翻译： 蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA） 生产。 结果表明，活性TRACP5b是从蚕生产的。 这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。

公开(公告)号：US20110239313A1

公开(公告)日：2011-09-29

申请号：US12521701

申请日：2007-12-28

申请人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

发明人： Iwao Kiyokawa ,  Tatsuya Ohashi ,  Toshihide Miura ,  Katsuhiro Katayama ,  Toshiki Tamura ,  Isao Kobayashi ,  Hideki Sezutsu

IPC分类号： C12P21/00 ,  C12N15/85 ,  A01K67/04

CPC分类号： C12N9/16

摘要： Silkworms which have (i) a DNA encoding a transcriptional regulator operably linked downstream of a promoter of a DNA encoding a protein specifically expressed in the silk gland and (ii) a DNA encoding TRACP5 operably linked downstream of a target promoter of the transcriptional regulator were produced. The result showed that active TRACP5b was produced from the silkworms. This means that TRACP5 produced from the silk gland of the silkworms undergoes processing in the silk gland that is similar to the processing taking place at bone resorption sites.

摘要翻译： 蚕具有（i）编码转录调节子的DNA，其可操作地连接在编码丝腺特异性表达的蛋白质的DNA的启动子的下游，和（ii）可操作地连接在转录调节子的靶启动子下游的编码TRACP5的DNA） 生产。 结果表明，活性TRACP5b由蚕生产。 这意味着从蚕丝腺产生的TRACP5在丝腺中进行加工，类似于在骨吸收部位发生的加工。

公开(公告)号：US08865966B2

公开(公告)日：2014-10-21

申请号：US13701946

申请日：2011-05-30

申请人： Iwao Kiyokawa ,  Yuji Arimatsu ,  Toshihide Miura ,  Ryo Kojima ,  Hideki Sezutsu ,  Keiro Uchino ,  Isao Kobayashi ,  Toshiki Tamura

发明人： Iwao Kiyokawa ,  Yuji Arimatsu ,  Toshihide Miura ,  Ryo Kojima ,  Hideki Sezutsu ,  Keiro Uchino ,  Isao Kobayashi ,  Toshiki Tamura

IPC分类号： C12P21/00 ,  A01K67/033 ,  C12N15/00 ,  C12N15/85 ,  C12N15/09 ,  A01K67/04 ,  C12P21/02

CPC分类号： C12N15/8509 ,  A01K67/033 ,  A01K67/0335 ,  A01K67/04 ,  A01K2217/052 ,  A01K2227/706 ,  A01K2267/01 ,  C12N15/09 ,  C12P21/02

摘要： Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.

摘要翻译： 通过将编码单体CRP的DNA转移到蚕中，从而构建转基因蚕，然后收集和纯化由上述构建的转基因蚕产生的五聚体CRP，从而高效生产五聚体CRP。

公开(公告)号：US20130212717A1

公开(公告)日：2013-08-15

申请号：US13701946

申请日：2011-05-30

申请人： Iwao Kiyokawa ,  Yuji Arimatsu ,  Toshihide Miura ,  Ryo Kojima ,  Hideki Sezutsu ,  Keiro Uchino ,  Isao Kobayashi ,  Toshiki Tamura

发明人： Iwao Kiyokawa ,  Yuji Arimatsu ,  Toshihide Miura ,  Ryo Kojima ,  Hideki Sezutsu ,  Keiro Uchino ,  Isao Kobayashi ,  Toshiki Tamura

IPC分类号： C12N15/85 ,  A01K67/033

CPC分类号： C12N15/8509 ,  A01K67/033 ,  A01K67/0335 ,  A01K67/04 ,  A01K2217/052 ,  A01K2227/706 ,  A01K2267/01 ,  C12N15/09 ,  C12P21/02

摘要： Pentameric CRP is produced at a high efficiency by transferring DNA, which encodes monomeric CRP, into a silkworm to thereby construct a transgenic silkworm and then collecting and purifying pentameric CRP that is produced by the transgenic silkworm constructed above.

摘要翻译： 通过将编码单体CRP的DNA转移到蚕中，从而构建转基因蚕，然后收集和纯化由上述构建的转基因蚕产生的五聚体CRP，从而高效生产五聚体CRP。

公开(公告)号：US07465556B2

公开(公告)日：2008-12-16

申请号：US10546608

申请日：2004-02-20

申请人： Toshihide Miura ,  Tatsuya Ohashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

发明人： Toshihide Miura ,  Tatsuya Ohashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

IPC分类号： C12Q1/42 ,  G01N33/573 ,  G01N33/53 ,  G01N33/535

CPC分类号： G01N33/573 ,  Y10S436/815

摘要： Specific and accurate immunoassay of tartrate resistant acid phosphatase 5b (TRACP 5b) in a specimen may be carried out by bonding TRACP 5b in the specimen to an antibody, subjecting the TRACP 5b bonded to the antibody to enzyme reaction with a 2-halo-4-nitrophenylphosphoric acid or a salt thereof, a substrate for TRACP 5b, and then assaying the enzymatic activity of TRACP 5b.

摘要翻译： 样品中酒石酸盐酸性磷酸酶5b（TRACP 5b）的特异性和准确的免疫测定可以通过将样品中的TRACP 5b结合到抗体上进行，将与抗体结合的TRACP 5b与2-卤代-4 - 硝基苯基磷酸或其盐，TRACP 5b的底物，然后测定TRACP 5b的酶活性。

公开(公告)号：US20060154317A1

公开(公告)日：2006-07-13

申请号：US10546608

申请日：2004-02-20

申请人： Toshihide Miura ,  Tatsuya Ohashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

发明人： Toshihide Miura ,  Tatsuya Ohashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

IPC分类号： G01N33/537 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/573 ,  Y10S436/815

摘要： Specific and accurate immunoassay of tartrate resistant acid phosphatase 5b (TRACP 5b) in a specimen may be carried out by bonding TRACP 5b in the specimen to an antibody, subjecting the TRACP 5b bonded to the antibody to enzyme reaction with a 2-halo-4-nitrophenylphosphoric acid or a salt thereof, a substrate for TRACP 5b, and then assaying the enzymatic activity of TRACP 5b.

摘要翻译： 样品中酒石酸盐酸性磷酸酶5b（TRACP 5b）的特异性和准确的免疫测定可以通过将样品中的TRACP 5b结合到抗体上进行，将与抗体结合的TRACP 5b与2-卤代-4 - 硝基苯基磷酸或其盐，TRACP 5b的底物，然后测定TRACP 5b的酶活性。

公开(公告)号：US07074903B2

公开(公告)日：2006-07-11

申请号：US10424726

申请日：2003-04-29

申请人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

发明人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Kumiko Sasagawa ,  Katsuhiro Katayama

IPC分类号： C07K16/40 ,  C12N5/20 ,  C12P21/08 ,  G01N33/543 ,  G01N33/573

CPC分类号： C07K16/40 ,  Y10S435/96 ,  Y10S435/975

摘要： Monocolonal antibodies having a higher reactivity with tartrate-resistant acid phosphatase 5b (TRACP 5b) than tartrate-resistant acid phosphatase 5a (TRACP 5a) and having a higher specificity to TRACP 5b can be obtained by cell fusion using as antigens TRACP 5b purified from human osteoclasts. By using the monoclonal antibody, TRACP 5b in a sample can be detected specifically with a high sensitivity.

公开(公告)号：US07611855B2

公开(公告)日：2009-11-03

申请号：US10538912

申请日：2003-12-24

申请人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Fumio Nomura ,  Takeshi Tomonaga ,  Katsuhiro Katayama

发明人： Tatsuya Ohashi ,  Toshihide Miura ,  Yoshihiko Igarashi ,  Fumio Nomura ,  Takeshi Tomonaga ,  Katsuhiro Katayama

IPC分类号： G01N33/543 ,  G01N33/546 ,  G01N33/573 ,  C07K16/40 ,  C12P21/08 ,  C12N5/20

CPC分类号： G01N33/54393 ,  Y10S435/962 ,  Y10S435/975 ,  Y10S436/811

摘要： Using two types of antibodies, i.e., a first antibody having a higher affinity for a target substance than for a competitive substance and a second antibody having a higher affinity for the competitive substance than for the target substance, a specimen is treated with these two antibodies. Then, the competitive substance in the specimen first binds to the second antibody and thus the ratio of the target substance to the competitive substance in the specimen is enlarged. As a result, the target substance becomes liable to bind to the first antibody and, in its turn, the reactivity of the target substance is elevated compared with the case of using the first antibody alone. Thus, the target substance in the specimen can be accurately assayed while avoiding the effects of the competitive substance contained in the specimen.

摘要翻译： 使用两种类型的抗体，即对靶物质的亲和力高于竞争性物质的第一抗体和对于竞争性物质具有比目标物质更高的亲和力的第二抗体，用这两种抗体 。 然后，样品中的竞争性物质首先与第二抗体结合，从而扩大了样品中目标物质与竞争性物质的比例。 结果，与仅使用第一抗体的情况相比，靶物质变得易于与第一抗体结合，并且反过来，靶物质的反应性升高。 因此，可以准确地测定样品中的目标物质，同时避免样品中所含的竞争性物质的影响。

公开(公告)号：US20120276562A1

公开(公告)日：2012-11-01

申请号：US13502198

申请日：2010-10-13

申请人： Wataru Kikuchi ,  Momoe Sato ,  Kenta Noda ,  Iwao Kiyokawa ,  Toshihide Miura ,  Ryo Kojima ,  Fumio Nomura ,  Kazuyuki Sogawa

发明人： Wataru Kikuchi ,  Momoe Sato ,  Kenta Noda ,  Iwao Kiyokawa ,  Toshihide Miura ,  Ryo Kojima ,  Fumio Nomura ,  Kazuyuki Sogawa

IPC分类号： G01N33/566 ,  C07K16/00 ,  G01N21/47 ,  G01N33/545 ,  G01N21/78

CPC分类号： G01N33/6893 ,  C07K16/36 ,  C07K2317/34 ,  G01N33/5308 ,  G01N2333/75 ,  G01N2800/08

摘要： Disclosed is an immunoassay method whereby a 5.9 kDa peptide which results from the degradation of the α-E chain and α chain of human fibrinogens and which is used as a peptide marker for diagnosing hepatic disease can be specifically assayed in a biological sample containing contaminating peptides by bringing antibodies that recognize the N terminal of said peptide marker and antibodies that recognize the C terminal of said peptide marker into contact with said peptide marker, forming immune complexes of said peptide marker and the two antibodies, and immunoassaying the obtained immune complexes.

摘要翻译： 公开了一种免疫测定方法，其中可以在含有污染肽的生物样品中特异性地测定由于人纤维蛋白原的α-E链和α链的降解而产生的并用作诊断肝脏疾病的肽标志物的5.9kDa肽 通过将识别所述肽标记的N末端的抗体和识别所述肽标记的C末端的抗体与所述肽标记接触，形成所述肽标记和两种抗体的免疫复合物，并免疫测定所获得的免疫复合物。

公开(公告)号：US09017959B2

公开(公告)日：2015-04-28

申请号：US13502198

申请日：2010-10-13

申请人： Wataru Kikuchi ,  Momoe Sato ,  Kenta Noda ,  Iwao Kiyokawa ,  Toshihide Miura ,  Ryo Kojima ,  Fumio Nomura ,  Kazuyuki Sogawa

发明人： Wataru Kikuchi ,  Momoe Sato ,  Kenta Noda ,  Iwao Kiyokawa ,  Toshihide Miura ,  Ryo Kojima ,  Fumio Nomura ,  Kazuyuki Sogawa

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/68 ,  C07K16/36

CPC分类号： G01N33/6893 ,  C07K16/36 ,  C07K2317/34 ,  G01N33/5308 ,  G01N2333/75 ,  G01N2800/08

摘要： Disclosed is an immunoassay method whereby a 5.9 kDa peptide which results from the degradation of the α-E chain and α chain of human fibrinogens and which is used as a peptide marker for diagnosing hepatic disease can be specifically assayed in a biological sample containing contaminating peptides by bringing antibodies that recognize the N terminal of said peptide marker and antibodies that recognize the C terminal of said peptide marker into contact with said peptide marker, forming immune complexes of said peptide marker and the two antibodies, and immunoassaying the obtained immune complexes.

摘要翻译： 公开了一种免疫测定方法，其中可以在含有污染肽的生物样品中特异性地测定由于人纤维蛋白原的α-E链和α链的降解而产生的并用作诊断肝脏疾病的肽标志物的5.9kDa肽 通过将识别所述肽标记的N末端的抗体和识别所述肽标记的C末端的抗体与所述肽标记接触，形成所述肽标记和两种抗体的免疫复合物，并免疫测定所获得的免疫复合物。