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    POLYUNSATURATED FATTY ACID PRODUCTION IN HETEROLOGOUS ORGANISMS USING PUFA POLYKETIDE SYNTHASE SYSTEMS
    5.
    发明申请
    POLYUNSATURATED FATTY ACID PRODUCTION IN HETEROLOGOUS ORGANISMS USING PUFA POLYKETIDE SYNTHASE SYSTEMS 有权
    使用多不饱和脂多糖合成酶系统进行异构体生物聚合脂肪酸生产

    公开(公告)号：US20070270494A1

    公开(公告)日：2007-11-22

    申请号：US11686856

    申请日：2007-03-15

    申请人： James Metz ,  Jerry Kuner ,  James Lippmeier

    发明人： James Metz ,  Jerry Kuner ,  James Lippmeier

    IPC分类号： A61K31/20 ,  A01H5/00 ,  C07H21/00 ,  C12N1/00 ,  C12N15/82 ,  C12N9/00 ,  C12N5/10 ,  C12N15/74 ,  C11B11/00 ,  A23D7/00

    CPC分类号： C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

    摘要： Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the organism, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

    摘要翻译： 公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用 一样。 还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物体表达的脂肪酸合酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加生物体中丙二酰辅酶A的水平，以及 一方面抑制KASII或KASIII。 除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

    Carotene Synthase Gene and Uses Therefor
    8.
    发明申请
    Carotene Synthase Gene and Uses Therefor 有权

    公开(公告)号：US20070269869A1

    公开(公告)日：2007-11-22

    申请号：US11557761

    申请日：2006-11-08

    申请人： Craig Weaver ,  James Metz ,  Jerry Kuner ,  Frank Overton

    发明人： Craig Weaver ,  James Metz ,  Jerry Kuner ,  Frank Overton

    IPC分类号： C12P19/00 ,  A01H5/00 ,  C12P7/64 ,  C11B11/00

    CPC分类号： C12N9/001 ,  C12N9/00 ,  C12N9/0004

    摘要： Described herein is a novel three domain gene from Schizochytrium, denoted carotene synthase, that encodes a protein with three different enzymatic activities: phytoene dehydrogenase (PD), phytoene synthase (PS), and lycopene cyclase (LC). Also described is the isolated gene encoding the carotene synthase, homologues thereof, the enzyme encoded by such gene, biologically active portions and homologues thereof, recombinant nucleic acid molecules, microorganisms and plants that have been genetically modified to increase or decrease the action of such gene, and methods of producing carotenoids and derivatives thereof or methods of producing microorganisms and lipid products lacking pigmentation using the knowledge of the carotene synthase described herein.

    Carotene Synthase Gene and Uses Therefor
    9.
    发明申请
    Carotene Synthase Gene and Uses Therefor 审中-公开
    胡萝卜素合酶基因及其用途

    公开(公告)号：US20070253933A1

    公开(公告)日：2007-11-01

    申请号：US11557743

    申请日：2006-11-08

    申请人： Craig Weaver ,  James Metz ,  Jerry Kuner ,  Frank Overton

    发明人： Craig Weaver ,  James Metz ,  Jerry Kuner ,  Frank Overton

    IPC分类号： C12P1/04 ,  A61K39/00 ,  C07H21/04 ,  C07K14/00 ,  C07K16/18 ,  C12N1/21 ,  C12N15/00

    CPC分类号： C12N9/001 ,  C12N9/00 ,  C12N9/0004

    摘要： Described herein is a novel three domain gene from Schizochytrium, denoted carotene synthase, that encodes a protein with three different enzymatic activities: phytoene dehydrogenase (PD), phytoene synthase (PS), and lycopene cyclase (LC). Also described is the isolated gene encoding the carotene synthase, homologues thereof, the enzyme encoded by such gene, biologically active portions and homologues thereof, recombinant nucleic acid molecules, microorganisms and plants that have been genetically modified to increase or decrease the action of such gene, and methods of producing carotenoids and derivatives thereof or methods of producing microorganisms and lipid products lacking pigmentation using the knowledge of the carotene synthase described herein.

    摘要翻译： 本文描述了来自裂殖酵母（Schizochytrium）的新型三结构域基因，表示为胡萝卜素合成酶，其编码具有三种不同酶活性的蛋白质：芸苔属脱氢酶（PD），八氢番茄红素合成酶（PS）和番茄红素环化酶（LC）。 还描述了编码胡萝卜素合成酶，其同系物，由该基因编码的酶，生物活性部分及其同源物，经遗传修饰以增加或减少该基因的作用的重组核酸分子，微生物和植物的分离基因 以及生产类胡萝卜素及其衍生物的方法或使用本文所述的胡萝卜素合酶的知识来生产缺乏色素沉淀的微生物和脂质产物的方法。