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    Method to transform algae, materials therefor, and products produced thereby
    1.
    发明授权
    Method to transform algae, materials therefor, and products produced thereby 失效
    转化藻类，其材料的方法和由此生产的产品

    公开(公告)号：US5661017A

    公开(公告)日：1997-08-26

    申请号：US404732

    申请日：1995-03-15

    申请人： Terri Goodman Dunahay ,  Paul G. Roessler ,  Eric E. Jarvis

    发明人： Terri Goodman Dunahay ,  Paul G. Roessler ,  Eric E. Jarvis

    IPC分类号： C12N9/00 ,  C12N15/29 ,  C12N15/82 ,  A01H13/00 ,  C12N1/13 ,  C12N15/31 ,  C12N15/79

    CPC分类号： C12N15/8241 ,  C12N9/93

    摘要： Disclosed is a method to transform chlorophyll C-containing algae which includes introducing a recombinant molecule comprising a nucleic acid molecule encoding a dominant selectable marker operatively linked to an algal regulatory control sequence into a chlorophyll C-containing alga in such a manner that the marker is produced by the alga. In a preferred embodiment the algal regulatory control sequence is derived from a diatom and preferably Cyclotella cryptica. Also disclosed is a chimeric molecule having one or more regulatory control sequences derived from one or more chlorophyll C-containing algae operatively linked to a nucleic acid molecule encoding a selectable marker, an RNA molecule and/or a protein, wherein the nucleic acid molecule does not normally occur with one or more of the regulatory control sequences. Further specifically disclosed are molecules pACCNPT10, pACCNPT4.8 and pACCNPT5.1. The methods and materials of the present invention provide the ability to accomplish stable genetic transformation of chlorophyll C-containing algae.

    摘要翻译： 公开了一种转化含叶绿素C的藻类的方法，其包括将包含编码与藻类调节控制序列有效连接的显性选择标记的核酸分子的重组分子引入含叶绿素C的藻类中，使得标记为 由藻类生产。 在优选的实施方案中，藻类调节控制序列衍生自硅藻，优选来自Cyclotella cryptica。 还公开了具有一种或多种调节控制序列的嵌合分子，所述调节控制序列衍生自与可选择标记，RNA分子和/或蛋白质编码的核酸分子有效连接的一种或多种含有叶绿素C的藻类，其中所述核酸分子 通常不会发生一个或多个调控序列。 进一步具体公开的是分子pACCNPT10，pACCNPT4.8和pACCNPT5.1。 本发明的方法和材料提供了完成叶绿素C的藻类的稳定遗传转化的能力。