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公开(公告)号：US11926865B2
公开(公告)日：2024-03-12
申请号：US17672552
申请日：2022-02-15
发明人： Nikolay Samusik , Garry P. Nolan , Yury Goltsev
IPC分类号： C12Q1/68 , C12Q1/6804 , C12Q1/6818
CPC分类号： C12Q1/6818 , C12Q1/6804 , C12Q1/6804 , C12Q2565/1015 , C12Q1/6804 , C12Q2565/101
摘要： A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
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公开(公告)号：US20210238665A1
公开(公告)日：2021-08-05
申请号：US17230706
申请日：2021-04-14
IPC分类号： C12Q1/6841 , C12Q1/682 , G01N33/542
摘要： SNAIL provides cost-efficient detection of specific nucleic acids in single cells, and may be combined with flow cytometry to simultaneously analyze large numbers of cells for a plurality of nucleic acids, e.g. at least one, to up to 5, up to 10, up to 15, up to 20 or more transcripts can be simultaneously analyzed, at a rate of up to about 50, 100, 250, 500 or more cells/second. The methods require only two primers for amplification, and may further include a detection primer.
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公开(公告)号：US10982263B2
公开(公告)日：2021-04-20
申请号：US16679769
申请日：2019-11-11
IPC分类号： C12Q1/68 , C12Q1/6818 , C12Q1/6804
摘要： A method for analyzing planar sample is provided. In some cases the method comprises: (a) labelling the planar sample with a capture agent that is linked to a nucleic acid, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the nucleic acid, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
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公开(公告)号：US20190330689A1
公开(公告)日：2019-10-31
申请号：US16240463
申请日：2019-01-04
发明人： Nikolay Samusik , Garry P. Nolan , Yury Goltsev
IPC分类号： C12Q1/6818 , C12Q1/6804
摘要： A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
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公开(公告)号：US10267802B2
公开(公告)日：2019-04-23
申请号：US15275043
申请日：2016-09-23
IPC分类号： G01N33/574
摘要： Cellular markers indicating a poor prognosis for ovarian cancer patients are disclosed. In particular, the invention relates to methods utilizing the frequency of a subset of cells in ovarian tumor tissue expressing vimentin, cMyc, or HE4, or any combination thereof, to predict an ovarian cancer patient will relapse.
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公开(公告)号：US10041949B2
公开(公告)日：2018-08-07
申请号：US14483999
申请日：2014-09-11
IPC分类号： G01N33/48 , G01N33/50 , G01N33/58 , H01J49/00 , G01N33/68 , G01N33/483 , G01N33/487 , G01N15/10
CPC分类号： G01N33/58 , G01N15/10 , G01N33/483 , G01N33/48735 , G01N33/5005 , G01N33/6848 , G01N2015/1006 , G01N2458/15 , H01J49/0004
摘要： A method of generating a high resolution two-dimensional image of a sample comprising cells and extracellular structures is provided. In certain embodiments, the method comprises: labeling a sample with at least one mass tag, thereby producing a labeled sample; scanning the sample with a secondary ion mass spectrometer (SIMS) ion beam to generate a data set that comprises spatially-addressable measurements of the abundance of the mass tag across an area of the sample; and outputting the data set. In many embodiments, the data set contains the identity and abundance of the mass tag. A system for performing the method is also provided.
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公开(公告)号：US09909167B2
公开(公告)日：2018-03-06
申请号：US14560921
申请日：2014-12-04
发明人： Nikolay Samusik , Garry P. Nolan , Yury Goltsev
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q1/6804 , C12Q2565/1015 , C12Q2565/101
摘要： A method for analyzing planar sample is provided. In some cases the method comprises: (a) incubating the planar sample with a capture agent that is linked to an oligonucleotide, wherein the capture agent specifically binds to complementary sites in the planar sample; (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the oligonucleotide, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
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公开(公告)号：US20170082628A1
公开(公告)日：2017-03-23
申请号：US15275043
申请日：2016-09-23
IPC分类号： G01N33/574
CPC分类号： G01N33/57449 , G01N2333/4703 , G01N2800/52 , G01N2800/54
摘要： Cellular markers indicating a poor prognosis for ovarian cancer patients are disclosed. In particular, the invention relates to methods utilizing the frequency of a subset of cells in ovarian tumor tissue expressing vimentin, cMyc, or HE4, or any combination thereof, to predict an ovarian cancer patient will relapse.
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9.发明授权Apparatus and method for sub-micrometer elemental image analysis by mass spectrometry 有权通过质谱法进行亚微米元素图像分析的装置和方法公开(公告)号：US09312111B2
公开(公告)日：2016-04-12
申请号：US14673279
申请日：2015-03-30
CPC分类号： H01J49/0036 , H01J49/0004 , H01J49/0409 , H01J49/0413 , H01J49/142 , H01J49/40 , H01J49/401
摘要： A mass spectrometer system having: a primary ion source capable of irradiating a segment on a planar sample with a beam of primary ions, an orthogonal ion mass-to-charge ratio, the analyzer being configured to separate secondary elemental atomic ions according to their mass-to-charge ratio by time of flight; an ion detector for detecting secondary elemental atomic ions and producing mass spectra measurements; and a synchronizer. In the system, the beam of primary ions scans across the planar sample in two dimensions and the synchronizer associates the mass spectra measurements with positions on the planar sample.
摘要翻译： 一种质谱仪系统，具有：一次离子源，其能够用一次离子束照射平面样品上的段，正交离子质荷比，所述分析器被配置为根据其质量分离次级元素原子离子 按飞行时间计算; 用于检测次要元素原子离子并产生质谱测量的离子检测器; 和同步器。 在系统中，主要离子束在两维中扫描平面样品，同步器将质谱测量与平面样品上的位置相关联。
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公开(公告)号：US20230065757A1
公开(公告)日：2023-03-02
申请号：US17795216
申请日：2021-02-04
IPC分类号： G01N33/50 , C07K16/28 , A61P35/00 , G01N33/569 , G01N33/574
摘要： Provided herein, among other things, is a method for predicting how a patient responds to immunotherapy. In some embodiments, the method may comprise: performing a multiplexed binding assay on a tissue section of a tumor obtained from a cancer patient to identify at least cancer cells, effector immune cells and immunosuppressive cells in the tissue section; measuring, for each cell of a plurality of the effector immune cells: (i) the physical distance to its most proximal cancer cell; and (ii) the physical distances to its most proximal immunosuppressive cell; and calculating, for each of the effector immune cells analyzed, the ratio of the distance measured in step (i) and distance measured in step (ii), wherein the ratio is predictive of the patient's response to immunotherapy. The method may be used to select patients for immunotherapy.
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