公开(公告)号：US20180002170A1

公开(公告)日：2018-01-04

申请号：US15463860

申请日：2017-03-20

申请人： The Regents of the University of California

发明人： R. Adam Seger ,  Paolo Actis ,  Boaz Vilozny ,  Nader Pourmand

IPC分类号： B82Y5/00 ,  G01Q60/44 ,  B82Y15/00 ,  B01L3/00 ,  G01N33/487

CPC分类号： B82Y5/00 ,  B01J2219/00371 ,  B01L3/502715 ,  B01L3/50273 ,  B01L2300/0645 ,  B82Y15/00 ,  G01N33/48728 ,  G01Q60/44

摘要： Disclosed herein are methods and systems for controlled ejection of desired material onto surfaces including in single cells using nanopipettes, as well as ejection onto and into cells. Some embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller for depositing a user defined pattern on an arbitrary substrate for the purpose of controlled cell adhesion and growth. Alternate embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller and electronic control of a voltage differential in a bore of the nanopipette electroosmotically injecting material into a cell in a high-throughput manner and with minimal damage to the cell. Yet other embodiments are directed to method and system comprising functionalized nanopipettes combined with scanning ion conductance microscopy for studying molecular interactions and detection of biomolecules inside a single living cell.

公开(公告)号：US10345260B2

公开(公告)日：2019-07-09

申请号：US14641064

申请日：2015-03-06

申请人： The Regents of the University of California

发明人： Nader Pourmand ,  Boaz Vilozny ,  Paolo Actis ,  R. Adam Seger

IPC分类号： G01N27/447 ,  B01L3/02 ,  B01L3/00 ,  B82Y35/00 ,  G01N33/487 ,  G01Q60/44

摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.

公开(公告)号：US20150177189A1

公开(公告)日：2015-06-25

申请号：US14641064

申请日：2015-03-06

申请人： The Regents of the University of California

发明人： Nader Pourmand ,  Boaz Vilozny ,  Paolo Actis ,  R. Adam Seger

IPC分类号： G01N27/447

CPC分类号： G01N27/44791 ,  B01L3/021 ,  B01L3/50273 ,  B01L2300/0896 ,  B82Y35/00 ,  G01N33/48721 ,  G01Q60/44

摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.

摘要翻译： 公开了用于检测离子迁移和结合的方法和装置，利用适用于电化学感测电路的纳米管。 可以在其内孔上用金属螯合剂官能化纳米针管，用于结合和感测金属离子或其它特异性结合分子，例如用于结合和感测葡萄糖的硼酸。 这种功能化的纳米移液管包含在电传感器中，该传感器检测纳米管片何时选择性地和可逆地结合离子或小分子。 还公开了一种纳米反应器，其包括纳米管，用于通过电压导向的离子迁移控制水溶液中的沉淀，其中离子可以通过孔中的排斥电荷引出到内孔内。

公开(公告)号：US20220229014A1

公开(公告)日：2022-07-21

申请号：US17557549

申请日：2021-12-21

申请人： The Regents of the University of California

发明人： Nader Pourmand ,  Boaz Vilozny ,  Paolo Actis ,  R. Adam Seger

IPC分类号： G01N27/447 ,  B01L3/02 ,  B01L3/00 ,  G01N33/487 ,  G01Q60/44 ,  G01N27/327 ,  B82Y35/00

摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.

公开(公告)号：US11243188B2

公开(公告)日：2022-02-08

申请号：US16422735

申请日：2019-05-24

申请人： The Regents of the University of California

发明人： Nader Pourmand ,  Boaz Vilozny ,  Paolo Actis ,  R. Adam Seger

IPC分类号： G01N27/327 ,  C25B7/00 ,  G01N27/447 ,  B01L3/02 ,  B01L3/00 ,  G01N33/487 ,  G01Q60/44 ,  B82Y35/00

摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.

公开(公告)号：US10513434B2

公开(公告)日：2019-12-24

申请号：US15463860

申请日：2017-03-20

申请人： The Regents of the University of California

发明人： R. Adam Seger ,  Paolo Actis ,  Boaz Vilozny ,  Nader Pourmand

IPC分类号： B82Y15/00 ,  B82Y5/00 ,  B01L3/00 ,  G01Q60/44 ,  G01N33/487

摘要： Disclosed herein are methods and systems for controlled ejection of desired material onto surfaces including in single cells using nanopipettes, as well as ejection onto and into cells. Some embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller for depositing a user defined pattern on an arbitrary substrate for the purpose of controlled cell adhesion and growth. Alternate embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller and electronic control of a voltage differential in a bore of the nanopipette electroosmotically injecting material into a cell in a high-throughput manner and with minimal damage to the cell. Yet other embodiments are directed to method and system comprising functionalized nanopipettes combined with scanning ion conductance microscopy for studying molecular interactions and detection of biomolecules inside a single living cell.

公开(公告)号：US11709148B2

公开(公告)日：2023-07-25

申请号：US17557549

申请日：2021-12-21

申请人： The Regents of the University of California

发明人： Nader Pourmand ,  Boaz Vilozny ,  Paolo Actis ,  R. Adam Seger

IPC分类号： B01L3/02 ,  G01N27/416 ,  G01N27/447 ,  B01L3/00 ,  G01N33/487 ,  G01Q60/44 ,  G01N27/327 ,  B82Y35/00

CPC分类号： G01N27/44791 ,  B01L3/021 ,  B01L3/50273 ,  B82Y35/00 ,  G01N27/3277 ,  G01N27/4167 ,  G01N33/48721 ,  G01Q60/44 ,  B01L2300/0896

摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.