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公开(公告)号：US11709148B2
公开(公告)日：2023-07-25
申请号：US17557549
申请日：2021-12-21
发明人： Nader Pourmand , Boaz Vilozny , Paolo Actis , R. Adam Seger
IPC分类号： B01L3/02 , G01N27/416 , G01N27/447 , B01L3/00 , G01N33/487 , G01Q60/44 , G01N27/327 , B82Y35/00
CPC分类号： G01N27/44791 , B01L3/021 , B01L3/50273 , B82Y35/00 , G01N27/3277 , G01N27/4167 , G01N33/48721 , G01Q60/44 , B01L2300/0896
摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.
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公开(公告)号：US20130143774A1
公开(公告)日：2013-06-06
申请号：US13693686
申请日：2012-12-04
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6874 , C12N15/10 , C12N15/1093 , C12Q1/6806 , C12Q2523/107 , C12Q2563/137
摘要： Provided are methods and compositions for the generation of broad size distributions of polynucleic acid fragments from larger polynucleic acids. The invention provides unbiased polynucleic acid fragments, i.e. fragments representative of all portions of the larger polynucleic acids. The methods are amendable to automation and are cost-effective. The methods comprise contacting the polynucleic acid sample with a reducing agent and a transition metal in a solvent to form a mixture. An exemplified reducing agent is sodium ascorbate, and an exemplified transition metal is copper.
摘要翻译： 提供了用于产生来自较大多核酸的多核酸片段的宽尺寸分布的方法和组合物。 本发明提供了无偏的多核酸片段，即代表较大多核苷酸的所有部分的片段。 这些方法可以自动化修改，具有成本效益。 所述方法包括使多核酸样品与还原剂和过渡金属在溶剂中接触以形成混合物。 示例性的还原剂是抗坏血酸钠，例示的过渡金属是铜。
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公开(公告)号：US10345260B2
公开(公告)日：2019-07-09
申请号：US14641064
申请日：2015-03-06
发明人： Nader Pourmand , Boaz Vilozny , Paolo Actis , R. Adam Seger
IPC分类号： G01N27/447 , B01L3/02 , B01L3/00 , B82Y35/00 , G01N33/487 , G01Q60/44
摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.
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4.发明申请公开(公告)号：US20150177189A1
公开(公告)日：2015-06-25
申请号：US14641064
申请日：2015-03-06
发明人： Nader Pourmand , Boaz Vilozny , Paolo Actis , R. Adam Seger
IPC分类号： G01N27/447
CPC分类号： G01N27/44791 , B01L3/021 , B01L3/50273 , B01L2300/0896 , B82Y35/00 , G01N33/48721 , G01Q60/44
摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.
摘要翻译： 公开了用于检测离子迁移和结合的方法和装置，利用适用于电化学感测电路的纳米管。 可以在其内孔上用金属螯合剂官能化纳米针管，用于结合和感测金属离子或其它特异性结合分子，例如用于结合和感测葡萄糖的硼酸。 这种功能化的纳米移液管包含在电传感器中，该传感器检测纳米管片何时选择性地和可逆地结合离子或小分子。 还公开了一种纳米反应器，其包括纳米管，用于通过电压导向的离子迁移控制水溶液中的沉淀，其中离子可以通过孔中的排斥电荷引出到内孔内。
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公开(公告)号：US11243188B2
公开(公告)日：2022-02-08
申请号：US16422735
申请日：2019-05-24
发明人： Nader Pourmand , Boaz Vilozny , Paolo Actis , R. Adam Seger
IPC分类号： G01N27/327 , C25B7/00 , G01N27/447 , B01L3/02 , B01L3/00 , G01N33/487 , G01Q60/44 , B82Y35/00
摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.
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公开(公告)号：US20210041426A1
公开(公告)日：2021-02-11
申请号：US16969146
申请日：2019-02-11
发明人： Nader Pourmand
IPC分类号： G01N33/543 , G01N27/48 , B01L3/02
摘要： Provided are methods for simultaneously detecting analytes. The methods include exposing a tip of a nanosensor to a biological sample, measuring ionic current flow through the nanosensor tip to detect a first analyte and a second analyte in the biological sample, and distinguishing the first analyte from the second analyte based on a first diffusion limited current peak resulting from binding of a first specific binding member to the first analyte being distinguishable from a second diffusion limited current peak resulting from binding of a second specific binding member to the second analyte. Sensing apparatuses that find use, e.g., in practicing the methods of the present disclosure, are also provided.
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公开(公告)号：US10513434B2
公开(公告)日：2019-12-24
申请号：US15463860
申请日：2017-03-20
发明人： R. Adam Seger , Paolo Actis , Boaz Vilozny , Nader Pourmand
IPC分类号： B82Y15/00 , B82Y5/00 , B01L3/00 , G01Q60/44 , G01N33/487
摘要： Disclosed herein are methods and systems for controlled ejection of desired material onto surfaces including in single cells using nanopipettes, as well as ejection onto and into cells. Some embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller for depositing a user defined pattern on an arbitrary substrate for the purpose of controlled cell adhesion and growth. Alternate embodiments are directed to a method and system comprising nanopipettes combined with an xyz controller and electronic control of a voltage differential in a bore of the nanopipette electroosmotically injecting material into a cell in a high-throughput manner and with minimal damage to the cell. Yet other embodiments are directed to method and system comprising functionalized nanopipettes combined with scanning ion conductance microscopy for studying molecular interactions and detection of biomolecules inside a single living cell.
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公开(公告)号：US20180259480A1
公开(公告)日：2018-09-13
申请号：US15697289
申请日：2017-09-06
IPC分类号： G01N27/42 , G01N27/403 , G01N33/487
CPC分类号： G01N27/42 , G01N27/4035 , G01N33/48707
摘要： Disclosed are methods and devices for biomolecular detection, comprising a nanopipette, exemplified as a hollow inert, non-biological structure with a conical tip opening of nanoscale dimensions, suitable for holding an electrolyte solution which may contain an analyte such as a protein biomolecule to be detected as it is passed through the tip opening. Biomolecules are detected by specific reaction with peptide ligands chemically immobilized in the vicinity of the tip. Analytes which bind to the ligands cause a detectible change in ionic current. A sensitive detection circuit, using a feedback amplifier circuit, and alternating voltages is further disclosed. Detection of IL-10 at a concentration of 4ng/ml is also disclosed, as is detection of VEGF.
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公开(公告)号：US20220229014A1
公开(公告)日：2022-07-21
申请号：US17557549
申请日：2021-12-21
发明人： Nader Pourmand , Boaz Vilozny , Paolo Actis , R. Adam Seger
IPC分类号： G01N27/447 , B01L3/02 , B01L3/00 , G01N33/487 , G01Q60/44 , G01N27/327 , B82Y35/00
摘要： Disclosed are methods and devices for detection of ion migration and binding, utilizing a nanopipette adapted for use in an electrochemical sensing circuit. The nanopipette may be functionalized on its interior bore with metal chelators for binding and sensing metal ions or other specific binding molecules such as boronic acid for binding and sensing glucose. Such a functionalized nanopipette is comprised in an electrical sensor that detects when the nanopipette selectively and reversibly binds ions or small molecules. Also disclosed is a nanoreactor, comprising a nanopipette, for controlling precipitation in aqueous solutions by voltage-directed ion migration, wherein ions may be directed out of the interior bore by a repulsing charge in the bore.
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公开(公告)号：US11255814B2
公开(公告)日：2022-02-22
申请号：US16588326
申请日：2019-09-30
IPC分类号： G01N27/42 , G01N33/487 , G01N27/403
摘要： Disclosed are methods and devices for biomolecular detection, comprising a nanopipette, exemplified as a hollow inert, non-biological structure with a conical tip opening of nanoscale dimensions, suitable for holding an electrolyte solution which may contain an analyte such as a protein biomolecule to be detected as it is passed through the tip opening. Biomolecules are detected by specific reaction with peptide ligands chemically immobilized in the vicinity of the tip. Analytes which bind to the ligands cause a detectible change in ionic current. A sensitive detection circuit, using a feedback amplifier circuit, and alternating voltages is further disclosed. Detection of IL-10 at a concentration of 4 ng/ml is also disclosed, as is detection of VEGF.
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