公开(公告)号：US10203307B2

公开(公告)日：2019-02-12

申请号：US14729999

申请日：2015-06-03

申请人： The Regents of the University of California

发明人： Daojing Wang ,  Pan Mao ,  Rafael Gomez-Sjoberg ,  Hung-Ta Wang ,  Peidong Yang

IPC分类号： G01N30/06 ,  H01J49/04 ,  G01N30/60 ,  G01N30/72 ,  B01L3/00 ,  G01N30/88

摘要： Systems, methods, and apparatus provide integration of microscopic imaging and liquid chromatography-mass spectrometry on a microfluidic device. In one aspect, an apparatus includes a first wafer, a second wafer, and an emitter. The emitter is disposed between the first wafer and the second wafer. The first wafer defines a sample input hole. The first wafer and the second wafer define a first channel, the first channel including a first end and a second end. The first end of the first channel is proximate the sample input hole. The first channel is configured to contain separation media. The second end of the first channel is proximate the emitter.

公开(公告)号：US20150293063A1

公开(公告)日：2015-10-15

申请号：US14729999

申请日：2015-06-03

申请人： The Regents of the University of California

发明人： Daojing Wang ,  Pan Mao ,  Rafael Gomez-Sjoberg ,  Hung-Ta Wang ,  Peidong Yang

IPC分类号： G01N30/06 ,  G01N30/72

CPC分类号： G01N30/06 ,  B01L3/502753 ,  B01L2300/069 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/0887 ,  G01N30/6095 ,  G01N30/7233 ,  G01N30/7266 ,  G01N2030/8813 ,  H01J49/0431

摘要： This disclosure provides systems, methods, and apparatus for integration of microscopic imaging and liquid chromatography-mass spectrometry on a microfluidic device. In one aspect, an apparatus includes a first wafer, a second wafer, and an emitter. The emitter is disposed between the first wafer and the second wafer. The first wafer defines a sample input hole. The first wafer and the second wafer define a first channel, the first channel including a first end and a second end. The first end of the first channel is proximate the sample input hole. The first channel is configured to contain separation media. The second end of the first channel is proximate the emitter.

摘要翻译： 本公开提供用于将微观成像和液相色谱 - 质谱法整合到微流体装置上的系统，方法和装置。 一方面，一种装置包括第一晶片，第二晶片和发射极。 发射极设置在第一晶片和第二晶片之间。 第一个晶片定义了一个样品输入孔。 第一晶片和第二晶片限定第一通道，第一通道包括第一端和第二端。 第一通道的第一端靠近样品输入孔。 第一个通道配置为包含分离介质。 第一通道的第二端靠近发射器。

公开(公告)号：US09793477B2

公开(公告)日：2017-10-17

申请号：US14137840

申请日：2013-12-20

申请人： The Regents of the University of California

发明人： Daojing Wang ,  Pan Mao ,  Hung-Ta Wang ,  Peidong Yang

IPC分类号： H01L49/00 ,  B01D59/44 ,  G01N30/72 ,  B01D3/00 ,  H01J49/04 ,  H01J49/00 ,  H01J49/16 ,  B01L3/00

CPC分类号： H01L49/00 ,  B01D59/44 ,  B01L3/5027 ,  G01N30/72 ,  G01N30/7206 ,  H01J49/0018 ,  H01J49/04 ,  H01J49/167

摘要： The present invention provides for a structure comprising a plurality of emitters, wherein a first nozzle of a first emitter and a second nozzle of a second emitter emit in two directions that are not or essentially not in the same direction; wherein the walls of the nozzles and the emitters form a monolithic whole. The present invention also provides for a structure comprising an emitter with a sharpened end from which the emitter emits; wherein the emitters forms a monolithic whole. The present invention also provides for a fully integrated separation of proteins and small molecules on a silicon chip before the electrospray mass spectrometry analysis.