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公开(公告)号：US09814802B2
公开(公告)日：2017-11-14
申请号：US13874054
申请日：2013-04-30
CPC分类号： A61L27/3604 , A61L27/3683 , A61L27/3804 , A61L2430/18
摘要： A method of differentiating cells into CK19-positive cells capable of producing hair follicle-like and hair structure-like can include: providing a tissue scaffold; seeding cells into the scaffold, the cells being capable of differentiation; incubating the scaffold having the cells in a cell growth media; and incubating the scaffold having the cells in an osteogenic differentiation medium sufficient for CK19-positive cells to be generated in the scaffold. The tissue scaffold can be a decellularized Whartons' jelly matrix. The cell growth media excludes osteogenic differentiation components: dexamethasone, β-glycerophosphate, 1α,25-hydroxyvitamin D3, and ascorbic acid 2-phosphate. The osteogenic differentiation medium includes the osteogenic differentiation components. The cells can be mesenchymal cells, such as WJMSCs.
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公开(公告)号：US20220054255A1
公开(公告)日：2022-02-24
申请号：US17517027
申请日：2021-11-02
发明人： Michael Detamore , Lindsey Ott , Robert Weatherly
摘要： An implant can include a plurality of polymeric fibers associated together into a fibrous body. The fibrous body is capable of being shaped to fit a tracheal defect and capable of being secured in place by suture or by bioadhesive. The fibrous body can have aligned fibers (e.g., circumferentially aligned) or unaligned fibers. The fibrous body can be electrospun. The fibrous body can have a first characteristic in a first gradient distribution across at least a portion of the fibrous body. The fibrous body can include one or more structural reinforcing members, such as ribbon structural reinforcing members, which can be embedded in the plurality of fibers. The fibrous body can include one or more structural reinforcing members bonded to the fibers with liquid polymer as an adhesive, the liquid polymer having a substantially similar composition of the fibers.
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公开(公告)号：US20160235892A1
公开(公告)日：2016-08-18
申请号：US15024317
申请日：2014-09-25
发明人： Michael Detamore , Amanda Renth , Amanda Sutherland , Emily Beck , Richard Hopkins , Gabriel Converse
CPC分类号： A61L27/54 , A61K9/0024 , A61K35/32 , A61L27/20 , A61L27/3612 , A61L27/3687 , A61L27/3691 , A61L27/52 , A61L2300/606 , A61L2300/622 , A61L2430/02 , A61L2430/06
摘要： The present invention is related to compositions comprising decellularized cartilage tissue powder in the forms of paste, putty, hydrogel, and scaffolds, methods of making compositions, and methods of using these compositions for treating osteochondral defects and full- or partial-thickness cartilage defects.
摘要翻译： 本发明涉及包含糊状，油灰，水凝胶和支架形式的脱细胞软骨组织粉末的组合物，制备组合物的方法，以及使用这些组合物治疗骨软骨缺损和全部或部分厚度软骨缺损的方法。
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4.发明申请GENERATING CK19-POSITIVE CELLS WITH HAIR-LIKE STRUCTURES FROM WHARTONS JELLY 有权生成CK19-阳性细胞，其中含有类似结构的JELLY公开(公告)号：US20140148915A1
公开(公告)日：2014-05-29
申请号：US13874054
申请日：2013-04-30
IPC分类号： A61L27/36
CPC分类号： A61L27/3604 , A61L27/3683 , A61L27/3804 , A61L2430/18
摘要： A method of differentiating cells into CK19-positive cells capable of producing hair follicle-like and hair structure-like can include: providing a tissue scaffold; seeding cells into the scaffold, the cells being capable of differentiation; incubating the scaffold having the cells in a cell growth media; and incubating the scaffold having the cells in an osteogenic differentiation medium sufficient for CK19-positive cells to be generated in the scaffold. The tissue scaffold can be a decellularized Whartons' jelly matrix. The cell growth media excludes osteogenic differentiation components: dexamethasone, β-glycerophosphate, 1α,25-hydroxyvitamin D3, and ascorbic acid 2-phosphate. The osteogenic differentiation medium includes the osteogenic differentiation components. The cells can be mesenchymal cells, such as WJMSCs.
摘要翻译： 将细胞分化成能够产生毛囊样和毛发结构的CK19阳性细胞的方法可以包括：提供组织支架; 将细胞接种到支架中，细胞能够分化; 将具有细胞的支架在细胞生长培养基中孵育; 并将具有细胞的支架在成骨分化培养基中孵育，足以使CK19阳性细胞在支架中产生。 组织支架可以是脱细胞的沃顿饮料基质。 细胞生长培养基排除成骨分化成分：地塞米松，甘油磷酸酯，1α，25-羟基维生素D3和2-磷酸抗坏血酸。 成骨分化培养基包括成骨分化成分。 细胞可以是间充质细胞，如WJMSCs。
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公开(公告)号：US11432922B2
公开(公告)日：2022-09-06
申请号：US17517027
申请日：2021-11-02
发明人： Michael Detamore , Lindsey Ott , Robert Weatherly
摘要： An implant can include a plurality of polymeric fibers associated together into a fibrous body. The fibrous body is capable of being shaped to fit a tracheal defect and capable of being secured in place by suture or by bioadhesive. The fibrous body can have aligned fibers (e.g., circumferentially aligned) or unaligned fibers. The fibrous body can be electrospun. The fibrous body can have a first characteristic in a first gradient distribution across at least a portion of the fibrous body. The fibrous body can include one or more structural reinforcing members, such as ribbon structural reinforcing members, which can be embedded in the plurality of fibers. The fibrous body can include one or more structural reinforcing members bonded to the fibers with liquid polymer as an adhesive, the liquid polymer having a substantially similar composition of the fibers.
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公开(公告)号：US10722614B2
公开(公告)日：2020-07-28
申请号：US15024317
申请日：2014-09-25
发明人： Michael Detamore , Amanda Renth , Amanda Sutherland , Emily Beck , Richard Hopkins , Gabriel Converse
摘要： The present invention is related to compositions comprising decellularized cartilage tissue powder in the forms of paste, putty, hydrogel, and scaffolds, methods of making compositions, and methods of using these compositions for treating osteochondral defects and full- or partial-thickness cartilage defects.
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公开(公告)号：US20200015962A1
公开(公告)日：2020-01-16
申请号：US16570510
申请日：2019-09-13
发明人： Michael Detamore , Lindsey Ott , Robert Weatherly
摘要： An implant can include a plurality of polymeric fibers associated together into a fibrous body. The fibrous body is capable of being shaped to fit a tracheal defect and capable of being secured in place by suture or by bioadhesive. The fibrous body can have aligned fibers (e.g., circumferentially aligned) or unaligned fibers. The fibrous body can be electrospun. The fibrous body can have a first characteristic in a first gradient distribution across at least a portion of the fibrous body. The fibrous body can include one or more structural reinforcing members, such as ribbon structural reinforcing members, which can be embedded in the plurality of fibers. The fibrous body can include one or more structural reinforcing members bonded to the fibers with liquid polymer as an adhesive, the liquid polymer having a substantially similar composition of the fibers.
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公开(公告)号：US20170067018A1
公开(公告)日：2017-03-09
申请号：US15353244
申请日：2016-11-16
IPC分类号： C12N5/0793 , C12N15/113
CPC分类号： C12N5/062 , A61K35/30 , C12N15/113 , C12N2310/14 , C12N2310/51 , C12N2506/025 , C12N2510/00 , C12N2533/90
摘要： A method of transforming human cells into mechanosensory hair cells (MHCs), such as inner hear hair cells in the cochlea and vestibular organs, can include: causing human Wharton's jelly cells (hWJCs) to increase expression of or biological function of HATH1 so as to transform the hWJCs into MHCs. The method can include; administering a nucleic acid that encodes HATH1 to the hWJCs; causing inhibited expression of or biological function of HES1 and/or HES5 in the hWJCs; administering a nucleic acid that inhibits HES1 and/or a nucleic acid that inhibits HES5 to the hWJCs; causing inhibited expression of or biological function of HES1 and/or HES5 in the WJCs by administering a nucleic acid that inhibits HES1 and/or a nucleic acid that inhibits HES5; nucleic acids are administered includes a sequence of SEQ ID NO: 2, SEQ ID NO: 3, and/or SEQ ID NO: 4.
摘要翻译： 将人类细胞转化为机械感染毛细胞（MHC）的方法，例如耳蜗和前庭器官内部听到的毛细胞，可以包括：引起人类沃顿的果冻细胞（hWJCs）增加HATH1的表达或生物学功能，以便 将hWJCs转化为MHC。 该方法可以包括 向hWJCs施用编码HATH1的核酸; 导致hWJCs中HES1和/或HES5的表达或生物学功能受到抑制; 施用抑制HES1的核酸和/或对hWJC抑制HES5的核酸; 通过施用抑制HES1的核酸和/或抑制HES5的核酸来抑制WJCs中HES1和/或HES5的表达或生物学功能; 核酸被给予包括SEQ ID NO：2，SEQ ID NO：3和/或SEQ ID NO：4的序列。
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公开(公告)号：US20160038643A1
公开(公告)日：2016-02-11
申请号：US14918229
申请日：2015-10-20
发明人： Michael Detamore , Emily Beck , Stevin Gehrke , Cory Berkland
CPC分类号： A61L27/48 , A61L27/20 , A61L27/3817 , A61L27/383 , A61L27/3834 , A61L27/50 , A61L27/52 , A61L27/54 , A61L2430/06 , A61L2430/32 , C08L5/08
摘要： An implantable hydrogel precursor composition can include: a cross-linkable polymer matrix that is biocompatible; and a plurality of polymer particles in the cross-linkable polymer matrix. The cross-linkable polymer matrix can include a cross-linkable hyaluronic acid polymer that has cross-linkable functional groups. The hyaluronic acid polymer can be a methacrylated hyaluronic acid polymer. The methacrylated hyaluronic acid polymer can have a molecular weight from about 500 kDa to about 1.8 MDa. The polymer particles can include a cross-linked hyaluronic acid. The cross-linkable polymer matrix having the polymer particles has a yield stress. The cross-linkable polymer matrix having the polymer particles has shape retention at physiological temperatures. The composition can include live cells in the cross-linkable polymer matrix. The composition can include a biologically active agent in the cross-linkable polymer matrix.
摘要翻译： 可植入水凝胶前体组合物可以包括：可生物相容的可交联聚合物基质; 和可交联聚合物基质中的多个聚合物颗粒。 可交联聚合物基质可以包括具有可交联官能团的可交联的透明质酸聚合物。 透明质酸聚合物可以是甲基丙烯酸酯化的透明质酸聚合物。 甲基丙烯酸酯化的透明质酸聚合物可以具有约500kDa至约1.8MDa的分子量。 聚合物颗粒可以包括交联的透明质酸。 具有聚合物颗粒的可交联聚合物基质具有屈服应力。 具有聚合物颗粒的可交联聚合物基质在生理温度下具有形状保持性。 组合物可以包括可交联聚合物基质中的活细胞。 组合物可以在可交联的聚合物基质中包括生物活性剂。
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公开(公告)号：US10744228B2
公开(公告)日：2020-08-18
申请号：US15254709
申请日：2016-09-01
发明人： Michael Detamore , Emily Beck
摘要： An implantable composition can include methacrylated solubilized devitalized cartilage (MeSDVC) with or without devitalized cartilage (DVC) particles. These compositions can be hydrogel precursors. After implantation, the MeSDVC may be crosslinked so as to form a hydrogel. The crosslinked hydrogel can include the DVC particles. A hydrogel precursor matrix (e.g., not crosslinked) can include a crosslinkable substance that can be crosslinked into a hydrogel, where DVC particles are included in the precursor matrix. The hydrogel precursor matrix can be located in a tissue defect site, such as a hole or recess in a cartilage or bone, and then crosslinked into a hydrogel that has the DVC particles therein.
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