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    Methods for Mass Spectrometry of Mixtures of Protein or Polypeptides Using Proton Transfer Reaction
    3.
    发明申请
    Methods for Mass Spectrometry of Mixtures of Protein or Polypeptides Using Proton Transfer Reaction 审中-公开

    公开(公告)号：US20180102243A1

    公开(公告)日：2018-04-12

    申请号：US15830439

    申请日：2017-12-04

    申请人： Thermo Finnigan LLC

    发明人： James L. STEPHENSON, JR. ,  John E. P. SYKA ,  August A. SPECHT

    IPC分类号： H01J49/00 ,  H01J49/04 ,  C12Q1/04 ,  G01N33/68

    CPC分类号： H01J49/0072 ,  C12Q1/04 ,  G01N33/6848 ,  H01J49/0031 ,  H01J49/0445

    摘要： A method comprises: (1) making an extract of a biological sample; (2) repeatedly: (a) choosing a respective one of a plurality of pre-determined protein or polypeptide analyte compounds; (b) introducing a portion of the extract into an electrospray ionization source, thereby generating positive ions comprising a plurality of ion species; (c) isolating a plurality of subsets of the ion species comprising respective mass-to-charge (m/z) ratio ranges, each range including an m/z ratio corresponding to a respective protonation state of the chosen compound; (d) reacting the isolated plurality of subsets of first-generation ion species with proton transfer reaction reagent anions for a pre-determined time duration; (e) generating a mass spectrum of the product ion species; and (g) identifying either the presence or absence of the compound based on the mass spectrum; and (3) identifying the presence or absence of the microorganism within the sample based on analytes present.

    Methods for Data-Dependent Mass Spectrometry of Mixed Biomolecular Analytes
    5.
    发明申请
    Methods for Data-Dependent Mass Spectrometry of Mixed Biomolecular Analytes 审中-公开
    混合生物分子分析仪的数据相关质谱法

    公开(公告)号：US20160268112A1

    公开(公告)日：2016-09-15

    申请号：US15067727

    申请日：2016-03-11

    申请人： Thermo Finnigan LLC

    发明人： Ping F. YIP ,  James L. STEPHENSON, JR. ,  Oksana GVOZDYAK

    IPC分类号： H01J49/00 ,  G01N33/68

    CPC分类号： H01J49/0036 ,  G01N33/6848 ,  G01N33/6851 ,  H01J49/0031 ,  H01J49/004

    摘要： A method for mass spectral analysis of a sample containing a plurality of biomolecule species comprises: (a) mass analyzing a plurality of first-generation ion species generated from a sample portion; (b) automatically recognizing, for each of at least one biomolecule species, a respective subset of m/z ratios corresponding to respective first-generation ion species generated from the each biomolecule species; (c) selecting, from each recognized subset, a single representative m/z ratio; (d) isolating a sub-population of ions having each representative m/z ratio from ions having other m/z ratios; and (e) fragmenting each isolated sub-population of ions so as to generate second-generation ion species.

    摘要翻译： 包含多个生物分子物质的样品的质谱分析方法包括：（a）质量分析从样品部分产生的多个第一代离子物质; （b）对于至少一种生物分子物种中的每一种，自动识别对应于从每个生物分子物种产生的各自的第一代离子物种的m / z比率的相应子集; （c）从每个识别的子集中选择单个代表性的m / z比; （d）从具有其他m / z比的离子分离具有每个代表性m / z比的离子的子群; 和（e）将每个分离的离子子群体分段，以产生第二代离子物质。

    Methods for Data-Dependent Mass Spectrometry of Mixed Biomolecular Analytes
    6.
    发明申请
    Methods for Data-Dependent Mass Spectrometry of Mixed Biomolecular Analytes 审中-公开

    公开(公告)号：US20190164735A1

    公开(公告)日：2019-05-30

    申请号：US16262385

    申请日：2019-01-30

    申请人： Thermo Finnigan LLC

    发明人： Ping F. YIP ,  James L. STEPHENSON, JR. ,  Oksana GVOZDYAK

    IPC分类号： H01J49/00 ,  G01N33/68

    摘要： A method for mass spectral analysis of a sample containing a plurality of intact protein molecule species comprises: (a) mass analyzing a plurality of ion species generated from a sample portion; (b) automatically recognizing, for each of at least one intact protein molecule species, a respective subset of m/z ratios corresponding to ion species generated from the each intact protein molecule species; and (c) storing or reporting to a user information relating to each subset of the m/z ratios, wherein step (b) comprises: automatically assigning a tentative charge state to each above-threshold m/z ratio; automatically adjusting the tentative charge to achieve a set of self-consistent assigned charge states; and decomposing the assigned charge states into analyte-specific clusters, each analyte-specific cluster being a one of the subsets of the m/z ratios.

    Automated Determination of Mass Spectrometer Collision Energy
    7.
    发明申请
    Automated Determination of Mass Spectrometer Collision Energy 审中-公开

    公开(公告)号：US20180350578A1

    公开(公告)日：2018-12-06

    申请号：US15975634

    申请日：2018-05-09

    申请人： Thermo Finnigan LLC

    发明人： Ping F. YIP ,  Helene L. CARDASIS ,  James L. STEPHENSON, JR.

    IPC分类号： H01J49/00

    CPC分类号： H01J49/0045 ,  H01J49/0031 ,  H01J49/005

    摘要： The present disclosure establishes new dissociation parameters that may be used to determine the collision energy (CE) needed to achieve a desired extent of dissociation for a given analyte precursor ion using collision cell type collision-induced dissociation. This selection is based solely on the analyte precursor ion's molecular weight, MW, and charge state, z. Metrics are proposed that may be used as a parameter for the “extent of dissociation”, and then predictive models are developed of the CEs required to achieve a range of values for each metric. Each model is a simple smooth function of only MW and z of the precursor ion. Coupled with a real-time spectral deconvolution (m/z to mass) algorithm, methods in accordance with the invention enable control over the extent of dissociation through automated, real-time selection of collision energy in a precursor-dependent manner.