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    METHOD AND DEVICE FOR AUTOMATICALLY PROCESSING A SAMPLE
    2.
    发明申请
    METHOD AND DEVICE FOR AUTOMATICALLY PROCESSING A SAMPLE 有权
    用于自动处理样品的方法和装置

    公开(公告)号：US20120070823A1

    公开(公告)日：2012-03-22

    申请号：US12864641

    申请日：2009-01-13

    申请人： Thomas Rothmann ,  Ralf Himmelreich ,  Thomas Laue

    发明人： Thomas Rothmann ,  Ralf Himmelreich ,  Thomas Laue

    IPC分类号： C12Q1/68 ,  C12M1/12

    CPC分类号： B01L3/502715 ,  B01J2219/00335 ,  B01J2219/00373 ,  B01J2219/00423 ,  B01J2219/00605 ,  B01J2219/00641 ,  B01J2219/00722 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/10 ,  B01L2300/0672 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/1827 ,  C12M47/06

    摘要： The invention relates to a method and a device for processing a biological sample. According to the invention, the processing comprises binding, washing and eluting biomolecules of the biological sample. The aim of the invention is to provide a method and a device for the automatic processing of biological samples which can have a relatively large volume and which can be further process by a microfluidic system. According to the invention, a container (1) is provided with a filter (10) and can be or is closed. A biological sample is introduced into the container. An inlet or outlet (4) for liquids is mounted downstream of the filter. In order to carry out processing, liquids that are present in the container are not only sucked off through the filter and passed on via the inlet or outlet, but liquids required for processing are also pumped into the container through the filter. The container can be connected to a microfluidic system in a relatively easy manner since only one conduit is required for an automated processing.

    摘要翻译： 本发明涉及一种用于处理生物样品的方法和装置。 根据本发明，加工包括生物样品的结合，洗涤和洗脱生物分子。 本发明的目的是提供一种用于自动处理生物样品的方法和装置，其可以具有相对较大的体积并且可以通过微流体系统进一步处理。 根据本发明，容器（1）设置有过滤器（10）并且可以是或者是关闭的。 将生物样品引入容器中。 用于液体的入口或出口（4）安装在过滤器的下游。 为了进行处理，存在于容器中的液体不仅通过过滤器吸出并经由入口或出口通过，而且处理所需的液体也通过过滤器被泵送到容器中。 容器可以以相对容易的方式连接到微流体系统，因为仅需要一个管道用于自动化处理。

    SIMULTANEOUS DETECTION OF MULTIPLE NUCLEIC ACID SEQUENCES IN A REACTION
    3.
    发明申请
    SIMULTANEOUS DETECTION OF MULTIPLE NUCLEIC ACID SEQUENCES IN A REACTION 审中-公开
    在反应中同时检测多个核酸序列

    公开(公告)号：US20110151459A1

    公开(公告)日：2011-06-23

    申请号：US12991071

    申请日：2009-05-05

    申请人： Thomas Rothmann ,  Holger Engel ,  Thomas Laue

    发明人： Thomas Rothmann ,  Holger Engel ,  Thomas Laue

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q2527/107 ,  C12Q2537/143

    摘要： The present invention relates to a method for simultaneously amplifying and detecting nucleic acid sequences in a reaction comprising the following steps: (i) providing a sample comprising at least one nucleic acid molecule; (ii) providing reagents for performing an amplification reaction, wherein the reagents comprise at least four, preferably at least five, more preferably at least six probes, wherein (a) each of the probes is specific for a nucleic acid sequence; (b) at least two, preferably at least three probes carry the same label; and (c) each of the probes that carry the same label has a melting temperature (Tm) which differs by more than 2° C. from the other probes with the same label when they are dissociated from their target nucleic acid sequence by heating; (iii) amplifying the nucleic acid sequences in the reaction; (iv) detecting the amplified nucleic acids by determining whether the labeled probe has bound its nucleic acid sequence; and (v) detecting the temperature at which each given labeled probe dissociates from the nucleic acid sequence to which it has bound. The invention also relates to kits for the use in such a method.

    摘要翻译： 本发明涉及在反应中同时扩增和检测核酸序列的方法，包括以下步骤：（i）提供包含至少一种核酸分子的样品; （ii）提供用于进行扩增反应的试剂，其中试剂包含至少四个，优选至少五个，更优选至少六个探针，其中（a）每个探针对核酸序列是特异性的; （b）至少两个，优选至少三个探头携带相同的标签; 和（c）携带相同标记物的每个探针当通过加热从其靶核酸序列解离时，具有与其他具有相同标记的探针相差大于2℃的解链温度（Tm）; （iii）扩增反应中的核酸序列; （iv）通过确定标记的探针是否结合其核酸序列来检测扩增的核酸; 和（v）检测每个给定的标记探针从其所结合的核酸序列解离的温度。 本发明还涉及用于这种方法的试剂盒。

    Method for demonstrating the presence of molecules by means of optical gratings
    5.
    发明授权
    Method for demonstrating the presence of molecules by means of optical gratings 失效
    通过光栅显示分子的存在的方法

    公开(公告)号：US08780351B2

    公开(公告)日：2014-07-15

    申请号：US13319269

    申请日：2010-04-24

    申请人： Stefan Walheim ,  Thomas Schimmel ,  Steven Lenhert ,  Harald Fuchs ,  Falko Brinkmann ,  Thomas Laue

    发明人： Stefan Walheim ,  Thomas Schimmel ,  Steven Lenhert ,  Harald Fuchs ,  Falko Brinkmann ,  Thomas Laue

    IPC分类号： G01N21/00 ,  G01N21/55

    CPC分类号： G01N33/54373

    摘要： A method for detecting the presence of molecules includes bringing into contact with at least one optical grating comprised of a first liquid phase, a second liquid phase which cannot be mixed with the first liquid phase. The first liquid phase is arranged on a substrate in a form of periodic structures having a periodicity ranging from about 190 nm to about 10 μm. The molecules to be detected, which are capable of changing the optical characteristics of the at least one optical grating are added to the first liquid phase or to the second liquid phase either before, during or after the bringing step. The presence of molecules is detected by measuring a changed reflection or transmission of light at the at least one optical grating or a changing spatial course of the diffraction pattern as soon as the first liquid phase makes contact with the second liquid phase and with the molecules to be detected.

    摘要翻译： 用于检测分子存在的方法包括与至少一个光栅相接触，该光栅由第一液相，不能与第一液相混合的第二液相组成。 第一液相以周期性结构的形式布置在基板上，周期性范围为约190nm至约10μm。 能够改变至少一个光栅的光学特性的待检测分子在引入步骤之前，期间或之后被添加到第一液相或第二液相。 分子的存在通过在第一液相与第二液相接触并且分子到达时测量在至少一个光栅上的光的改变的反射或透射或衍射图的改变的空间过程来检测 被检测。

    METHOD FOR DEMONSTRATING THE PRESENCE OF MOLECULES BY MEANS OF OPTICAL GRATINGS
    6.
    发明申请
    METHOD FOR DEMONSTRATING THE PRESENCE OF MOLECULES BY MEANS OF OPTICAL GRATINGS 失效
    通过光学显微镜分析分子存在的方法

    公开(公告)号：US20120154817A1

    公开(公告)日：2012-06-21

    申请号：US13319269

    申请日：2010-04-24

    申请人： Stefan Walheim ,  Thomas Schimmel ,  Steven Lenhert ,  Harald Fuchs ,  Falko Brinkmann ,  Thomas Laue

    发明人： Stefan Walheim ,  Thomas Schimmel ,  Steven Lenhert ,  Harald Fuchs ,  Falko Brinkmann ,  Thomas Laue

    IPC分类号： G01N21/55

    CPC分类号： G01N33/54373

    摘要： A method for detecting the presence of molecules includes bringing into contact with at least one optical grating comprised of a first liquid phase, a second liquid phase which cannot be mixed with the first liquid phase. The first liquid phase is arranged on a substrate in a form of periodic structures having a periodicity ranging from about 190 nm to about 10 μm. The molecules to be detected, which are capable of changing the optical characteristics of the at least one optical grating are added to the first liquid phase or to the second liquid phase either before, during or after the bringing step. The presence of molecules is detected by measuring a changed reflection or transmission of light at the at least one optical grating or a changing spatial course of the diffraction pattern as soon as the first liquid phase makes contact with the second liquid phase and with the molecules to be detected.

    摘要翻译： 用于检测分子存在的方法包括与至少一个光栅相接触，该光栅由第一液相，不能与第一液相混合的第二液相组成。 第一液相以周期性结构的形式布置在基板上，周期性范围为约190nm至约10μm。 能够改变至少一个光栅的光学特性的待检测分子在引入步骤之前，期间或之后被添加到第一液相或第二液相。 分子的存在通过在第一液相与第二液相接触并且分子到达时测量在至少一个光栅上的光的改变的反射或透射或衍射图的改变的空间过程来检测 被检测。