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1.发明申请公开(公告)号：US20110151459A1
公开(公告)日：2011-06-23
申请号：US12991071
申请日：2009-05-05
申请人： Thomas Rothmann , Holger Engel , Thomas Laue
发明人： Thomas Rothmann , Holger Engel , Thomas Laue
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6818 , C12Q2527/107 , C12Q2537/143
摘要： The present invention relates to a method for simultaneously amplifying and detecting nucleic acid sequences in a reaction comprising the following steps: (i) providing a sample comprising at least one nucleic acid molecule; (ii) providing reagents for performing an amplification reaction, wherein the reagents comprise at least four, preferably at least five, more preferably at least six probes, wherein (a) each of the probes is specific for a nucleic acid sequence; (b) at least two, preferably at least three probes carry the same label; and (c) each of the probes that carry the same label has a melting temperature (Tm) which differs by more than 2° C. from the other probes with the same label when they are dissociated from their target nucleic acid sequence by heating; (iii) amplifying the nucleic acid sequences in the reaction; (iv) detecting the amplified nucleic acids by determining whether the labeled probe has bound its nucleic acid sequence; and (v) detecting the temperature at which each given labeled probe dissociates from the nucleic acid sequence to which it has bound. The invention also relates to kits for the use in such a method.
摘要翻译： 本发明涉及在反应中同时扩增和检测核酸序列的方法，包括以下步骤：（i）提供包含至少一种核酸分子的样品; （ii）提供用于进行扩增反应的试剂，其中试剂包含至少四个，优选至少五个，更优选至少六个探针，其中（a）每个探针对核酸序列是特异性的; （b）至少两个，优选至少三个探头携带相同的标签; 和（c）携带相同标记物的每个探针当通过加热从其靶核酸序列解离时，具有与其他具有相同标记的探针相差大于2℃的解链温度（Tm）; （iii）扩增反应中的核酸序列; （iv）通过确定标记的探针是否结合其核酸序列来检测扩增的核酸; 和（v）检测每个给定的标记探针从其所结合的核酸序列解离的温度。 本发明还涉及用于这种方法的试剂盒。
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公开(公告)号：US20120107818A1
公开(公告)日：2012-05-03
申请号：US13318424
申请日：2010-05-04
申请人： Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wendy , Rainer Dahlke
发明人： Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wendy , Rainer Dahlke
CPC分类号： C12Q1/6818 , Y02A50/59 , C12Q2527/107 , C12Q2537/143 , C12Q2565/629
摘要： The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising the following steps, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set, wherein (a) each probe set consists of at least three probes, (b) each of the probes is specific for a nucleic acid sequence, (c) there are at least two probes in each set which carry an identical label, (d) each of the probes in a given probe set that carries an identical label has a melting temperature (Tm) which differs by more than 2° C. from the other probe in said probe set with the same label, (e) wherein the probes carrying the identical label differ in melting temperature (Tm) in a way that they are distinguishable by melting point, (f) performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.
摘要翻译： 本发明涉及用于扩增和检测反应盒中的核酸序列的方法，包括以下步骤：（i）提供包含至少一种核酸分子的样品，（ii）在所述盒的第一反应室中提供试剂 用于扩增反应，（iii）将样品与扩增试剂混合，（iv）扩增筒的第一反应室中的至少一种核酸，（v）将至少部分扩增反应转移到第二和 每个探针组由至少三个探针组成，（b）每个探针对于核酸序列是特异性的，（c）存在至少两个探针 在每个携带相同标签的组中，（d）给定探针组中携带相同标记物的每个探针具有与所述探针中的另一个探针相差大于2℃的熔解温度（Tm） 设置相同的标签，（e）其中携带相同标记的探针以熔点可区分的方式在熔融温度（Tm）上不同，（f）进行熔点分析以确定哪个探针 已经特异地结合核酸。
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公开(公告)号：US08937174B2
公开(公告)日：2015-01-20
申请号：US12864641
申请日：2009-01-13
申请人： Thomas Rothmann , Ralf Himmelreich , Thomas Laue
发明人： Thomas Rothmann , Ralf Himmelreich , Thomas Laue
CPC分类号： B01L3/502715 , B01J2219/00335 , B01J2219/00373 , B01J2219/00423 , B01J2219/00605 , B01J2219/00641 , B01J2219/00722 , B01L7/52 , B01L2200/025 , B01L2200/027 , B01L2200/10 , B01L2300/0672 , B01L2300/0681 , B01L2300/0816 , B01L2300/1827 , C12M47/06
摘要： The invention relates to a method and a device for the automatic processing of biological samples which can have a relatively large volume and which can be further processed by a microfluidic system. A container is provided with a filter and can be or is closed. A biological sample is introduced into the container. An inlet or outlet for liquids is mounted downstream of the filter. In order to carry out processing, liquids that are present in the container are not only sucked off through the filter and passed on via the inlet or outlet, but liquids required for processing are also pumped into the container through the filter. The container can be connected to a microfluidic system in a relatively easy manner since only one conduit is required for an automated processing.
摘要翻译： 本发明涉及一种用于自动处理生物样品的方法和装置，其可以具有相对较大的体积并且可以由微流体系统进一步处理。 容器设有过滤器，可以被关闭。 将生物样品引入容器中。 用于液体的入口或出口安装在过滤器的下游。 为了进行处理，存在于容器中的液体不仅通过过滤器吸出并经由入口或出口通过，而且处理所需的液体也通过过滤器被泵送到容器中。 容器可以以相对容易的方式连接到微流体系统，因为仅需要一个管道用于自动化处理。
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公开(公告)号：US08852863B2
公开(公告)日：2014-10-07
申请号：US13318424
申请日：2010-05-04
申请人： Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wende , Rainer Dahlke
发明人： Thomas Rothmann , Holger Engel , Ralf Himmelreich , Andy Wende , Rainer Dahlke
CPC分类号： C12Q1/6818 , Y02A50/59 , C12Q2527/107 , C12Q2537/143 , C12Q2565/629
摘要： The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set and performing a melting point analysis in order to determine which of the probes has specifically bound a nucleic acid.
摘要翻译： 本发明涉及用于扩增和检测反应盒中的核酸序列的方法，其包括：（i）提供包含至少一个核酸分子的样品，（ii）在所述盒的第一反应室中提供用于扩增的试剂 反应，（iii）将样品与扩增试剂混合，（iv）扩增筒的第一反应室中的至少一种核酸，（v）将至少部分扩增反应转移到第二和第三反应室 的每个包含探针组并进行熔点分析，以便确定哪个探针具体结合核酸。
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公开(公告)号：US20120070823A1
公开(公告)日：2012-03-22
申请号：US12864641
申请日：2009-01-13
申请人： Thomas Rothmann , Ralf Himmelreich , Thomas Laue
发明人： Thomas Rothmann , Ralf Himmelreich , Thomas Laue
CPC分类号： B01L3/502715 , B01J2219/00335 , B01J2219/00373 , B01J2219/00423 , B01J2219/00605 , B01J2219/00641 , B01J2219/00722 , B01L7/52 , B01L2200/025 , B01L2200/027 , B01L2200/10 , B01L2300/0672 , B01L2300/0681 , B01L2300/0816 , B01L2300/1827 , C12M47/06
摘要： The invention relates to a method and a device for processing a biological sample. According to the invention, the processing comprises binding, washing and eluting biomolecules of the biological sample. The aim of the invention is to provide a method and a device for the automatic processing of biological samples which can have a relatively large volume and which can be further process by a microfluidic system. According to the invention, a container (1) is provided with a filter (10) and can be or is closed. A biological sample is introduced into the container. An inlet or outlet (4) for liquids is mounted downstream of the filter. In order to carry out processing, liquids that are present in the container are not only sucked off through the filter and passed on via the inlet or outlet, but liquids required for processing are also pumped into the container through the filter. The container can be connected to a microfluidic system in a relatively easy manner since only one conduit is required for an automated processing.
摘要翻译： 本发明涉及一种用于处理生物样品的方法和装置。 根据本发明，加工包括生物样品的结合，洗涤和洗脱生物分子。 本发明的目的是提供一种用于自动处理生物样品的方法和装置，其可以具有相对较大的体积并且可以通过微流体系统进一步处理。 根据本发明，容器（1）设置有过滤器（10）并且可以是或者是关闭的。 将生物样品引入容器中。 用于液体的入口或出口（4）安装在过滤器的下游。 为了进行处理，存在于容器中的液体不仅通过过滤器吸出并经由入口或出口通过，而且处理所需的液体也通过过滤器被泵送到容器中。 容器可以以相对容易的方式连接到微流体系统，因为仅需要一个管道用于自动化处理。
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公开(公告)号：US20130210019A1
公开(公告)日：2013-08-15
申请号：US13817174
申请日：2011-08-16
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12Q2521/301 , C12Q2521/513
摘要： The present invention relates to a method for amplifying a template nucleic acid, wherein the method comprises amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction in the presence of a nicking endonuclease, and wherein said template nucleic acid comprises a sequence recognized by said nicking endonuclease or a sequence recognized by said nicking endonuclease is introduced into the template nucleic acid during the HDA reaction. The invention further pertains to a kit for amplifying a nucleic acid, comprising a nicking endonuclease, a helicase and a DNA polymerase.
摘要翻译： 本发明涉及用于扩增模板核酸的方法，其中所述方法包括在切口内切核酸酶存在下使用解旋酶依赖性扩增（HDA）反应扩增所述模板核酸，并且其中所述模板核酸包含识别的序列 通过所述切口内切核酸酶或由所述切口内切核酸酶识别的序列在HDA反应期间引入模板核酸。 本发明还涉及用于扩增核酸的试剂盒，其包含切口内切核酸酶，解旋酶和DNA聚合酶。
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公开(公告)号：US20100267081A1
公开(公告)日：2010-10-21
申请号：US12671354
申请日：2008-08-01
摘要： The present invention provides a method for fixing and/or stabilizing a sample, in which the sample is put into a permeable container with a maximum overall height of 10 mm, preferably of 5 mm, and the container filled with the sample is immersed in fixing and/or stabilizing agents and the sample is fixed and/or stabilized.
摘要翻译： 本发明提供一种用于固定和/或稳定样品的方法，其中将样品放入具有最大总高度为10mm，优选为5mm的可渗透容器中，并且将填充有样品的容器浸入固定 和/或稳定剂，并且样品是固定和/或稳定的。
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公开(公告)号：US20100255571A1
公开(公告)日：2010-10-07
申请号：US12671369
申请日：2008-08-01
摘要： The present invention provides a method for fixing and/or stabilizing a sample, in which the sample is put into a permeable container, the permeable container having an opening, which is pressed into the sample, then a portion of the sample brought into the permeable container in this way is separated from the rest of the sample, and the container filled with the sample in this way is immersed in fixing and/or stabilizing agents and the sample is fixed and/or stabilized.
摘要翻译： 本发明提供了一种用于固定和/或稳定样品的方法，其中将样品放入可渗透容器中，该可渗透容器具有一个开口，该孔被压入样品中，然后一部分样品进入可渗透的 以这种方式将容器与样品的其余部分分离，并且以这种方式填充样品的容器浸入固定和/或稳定剂中，并且样品被固定和/或稳定。
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9.发明授权公开(公告)号：US09574225B2
公开(公告)日：2017-02-21
申请号：US13141593
申请日：2009-12-23
CPC分类号： C12Q1/6806 , C12N15/1013 , C12Q1/686 , C12Q2565/629 , C12Q2563/143 , C12Q2549/119 , C12Q2547/101
摘要： The invention relates to the preparation of a biological sample for performing verifications and examinations, wherein the aim of the invention is the creation of a method for preparing a biological sample having an improved PCR sensitivity compared to the reference standard having standard PCR without having to raise the cost thereof.
摘要翻译： 本发明涉及用于进行验证和检查的生物样品的制备，其中本发明的目的是创建与具有标准PCR的参考标准相比具有改进的PCR灵敏度的生物样品的制备方法，而不必提高 其成本。
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公开(公告)号：US08573518B2
公开(公告)日：2013-11-05
申请号：US12989569
申请日：2009-04-24
摘要： The invention relates to a method for breaking down biological material, especially for obtaining biomolecules by ultrasound, the biological material being arranged, in a container together with a liquid.
摘要翻译： 本发明涉及一种用于分解生物材料的方法，特别是通过超声波将生物分子与液体一起排列在容器中。
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