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    Semi-synthetic quorum sensors
    1.
    发明授权
    Semi-synthetic quorum sensors 有权
    半合成法定传感器

    公开(公告)号：US09102974B2

    公开(公告)日：2015-08-11

    申请号：US13248250

    申请日：2011-09-29

    申请人： Steven Lenhert

    发明人： Steven Lenhert

    IPC分类号： H04N7/18 ,  C12Q1/00 ,  G01N21/47 ,  G01N21/64

    CPC分类号： G01N21/6428 ,  C12Q1/001 ,  G01N21/4788 ,  G01N21/6452 ,  G01N21/6486 ,  H04N7/183

    摘要： Described is a device comprising a substrate, and a quorum sensor array on the substrate. The quorum sensor array comprises quorum sensors that release signal molecules in response to one or more environmental signals being sense by the quorum sensors to thereby amplify the one or more environmental signals by causing a signal chain reaction in neighboring quorum sensors of the quorum sensor array. Each of the quorum sensors comprises a lipid multilayer structure. Also described is a method comprising providing data for changes in optical properties of at least part of the quorum sensor array in response to exposing the quorum sensor array to one or more environmental signals, and determining the presence of the one or more environmental signals based on the data.

    摘要翻译： 描述了一种装置，其包括基板和基板上的法定传感器阵列。 该法定传感器阵列包括响应于一个或多个环境信号被法定传感器感知释放信号分子的法定传感器，从而通过在法定传感器阵列的相邻法定传感器中引起信号链反应来放大一个或多个环境信号。 每个法定传感器包括脂质多层结构。 还描述了一种方法，包括响应于将法定传感器阵列暴露于一个或多个环境信号而提供用于数量以改变至少部分法定传感器阵列的光学特性的数据，以及基于以下方式确定一个或多个环境信号的存在： 数据。

    LIPID MULTILAYER MICROARRAYS AND THEIR USE FOR CELL CULTURE SCREENING
    2.
    发明申请
    LIPID MULTILAYER MICROARRAYS AND THEIR USE FOR CELL CULTURE SCREENING 有权
    脂质多层微阵列及其用于细胞培养的筛选

    公开(公告)号：US20130137599A1

    公开(公告)日：2013-05-30

    申请号：US13534772

    申请日：2012-06-27

    申请人： STEVEN LENHERT ,  Aubrey Kusi-Appiah

    发明人： STEVEN LENHERT ,  Aubrey Kusi-Appiah

    IPC分类号： C12Q1/02

    CPC分类号： C12Q1/025 ,  B01L3/5088 ,  G01N33/5008 ,  G01N33/5432

    摘要： Provided is a device having one or more lipid multilayer arrays of lipid multilayer structures on a substrate. Each lipid multilayer structure encapsulates an encapsulated material that may be delivered to a cell that is in contact with the lipid multilayer structure to determine the cellular response of the cell to the encapsulated material.

    摘要翻译： 提供了一种在基底上具有一个或多个脂质多层结构脂质多层阵列的装置。 每个脂质多层结构包封可以递送至与脂质多层结构接触的细胞的封装材料，以确定细胞对包封材料的细胞反应。

    METHOD FOR DEMONSTRATING THE PRESENCE OF MOLECULES BY MEANS OF OPTICAL GRATINGS
    3.
    发明申请
    METHOD FOR DEMONSTRATING THE PRESENCE OF MOLECULES BY MEANS OF OPTICAL GRATINGS 失效
    通过光学显微镜分析分子存在的方法

    公开(公告)号：US20120154817A1

    公开(公告)日：2012-06-21

    申请号：US13319269

    申请日：2010-04-24

    申请人： Stefan Walheim ,  Thomas Schimmel ,  Steven Lenhert ,  Harald Fuchs ,  Falko Brinkmann ,  Thomas Laue

    发明人： Stefan Walheim ,  Thomas Schimmel ,  Steven Lenhert ,  Harald Fuchs ,  Falko Brinkmann ,  Thomas Laue

    IPC分类号： G01N21/55

    CPC分类号： G01N33/54373

    摘要： A method for detecting the presence of molecules includes bringing into contact with at least one optical grating comprised of a first liquid phase, a second liquid phase which cannot be mixed with the first liquid phase. The first liquid phase is arranged on a substrate in a form of periodic structures having a periodicity ranging from about 190 nm to about 10 μm. The molecules to be detected, which are capable of changing the optical characteristics of the at least one optical grating are added to the first liquid phase or to the second liquid phase either before, during or after the bringing step. The presence of molecules is detected by measuring a changed reflection or transmission of light at the at least one optical grating or a changing spatial course of the diffraction pattern as soon as the first liquid phase makes contact with the second liquid phase and with the molecules to be detected.

    摘要翻译： 用于检测分子存在的方法包括与至少一个光栅相接触，该光栅由第一液相，不能与第一液相混合的第二液相组成。 第一液相以周期性结构的形式布置在基板上，周期性范围为约190nm至约10μm。 能够改变至少一个光栅的光学特性的待检测分子在引入步骤之前，期间或之后被添加到第一液相或第二液相。 分子的存在通过在第一液相与第二液相接触并且分子到达时测量在至少一个光栅上的光的改变的反射或透射或衍射图的改变的空间过程来检测 被检测。

    Massively parallel lithography with two-dimensional pen arrays
    4.
    发明申请
    Massively parallel lithography with two-dimensional pen arrays 失效
    大尺寸平行光刻与二维笔阵列

    公开(公告)号：US20090325816A1

    公开(公告)日：2009-12-31

    申请号：US12000456

    申请日：2007-12-12

    申请人： Chad A. Mirkin ,  Peng Sun ,  Yuhuang Wang ,  Steven Lenhert

    发明人： Chad A. Mirkin ,  Peng Sun ,  Yuhuang Wang ,  Steven Lenhert

    IPC分类号： C40B40/04 ,  C40B50/14

    CPC分类号： G03F7/0002 ,  G01Q80/00

    摘要： Massive parallel printing of structures and nanostructures, including lipids, at high speed with high resolution and high quality using two dimensional arrays comprising cantilevers and tip-based transfer of material to a surface. The array is designed so only tips touch the surface. This can be accomplished by long tips and bent cantilevers and alignment. An article comprising: a two-dimensional array of a plurality of cantilevers, wherein the array comprises a plurality of base rows, each base row comprising a plurality of cantilevers, wherein each of the cantilevers comprise tips at the cantilever end away from the base, wherein the number of cantilevers is greater than 250, and wherein the tips have an apex height relative to the cantilever of at least four microns, and a support for the array. Combinatorial arrays and bioarrays can be prepared. The arrays can be manufactured by micromachining methods.

    摘要翻译： 使用包括悬臂和基于尖端的材料转移到表面的二维阵列，以高分辨率和高质量的高速大规模平行印刷结构和纳米结构，包括脂质。 该阵列设计为只有尖端触摸表面。 这可以通过长尖端和弯曲的悬臂和对准来实现。 一种制品，包括：多个悬臂的二维阵列，其中所述阵列包括多个基列，每个基列包括多个悬臂，其中每个所述悬臂包括远离所述基座的所述悬臂端处的尖端， 其中所述悬臂的数量大于250，并且其中所述尖端具有相对于至少四微米的所述悬臂的顶点高度，以及所述阵列的支撑。 可以制备组合阵列和生物阵列。 阵列可以通过微加工方法制造。

    Method of applying membrane lipids to a substrate
    8.
    发明授权
    Method of applying membrane lipids to a substrate 有权
    将膜脂质应用于底物的方法

    公开(公告)号：US08227033B2

    公开(公告)日：2012-07-24

    申请号：US11779351

    申请日：2007-07-18

    申请人： Steven Lenhert ,  Harald Fuchs

    发明人： Steven Lenhert ,  Harald Fuchs

    IPC分类号： B05D5/00 ,  B05D1/28

    CPC分类号： G03F7/0002 ,  B82Y10/00 ,  B82Y40/00 ,  Y10S977/713 ,  Y10S977/783 ,  Y10S977/849 ,  Y10S977/855 ,  Y10S977/857 ,  Y10S977/883

    摘要： A method for applying membrane lipids to a substrate includes providing a substrate and an ink reservoir having an ink including a membrane lipid. The tip of a scanning probe microscope is dipped into the ink so as to dispose the membrane lipid on the tip. The tip of the scanning probe microscope is brought into contact with a surface of the substrate. The tip is moved over regions of the surface so that the membrane lipid migrates from the tip of the scanning probe microscope onto the surface of the substrate in the regions and the membrane lipid organizes itself in the regions in a form of a single lipid layer or in a form of one or a plurality of mutually superposed lipid bilayers. The tip is removed from the surface of the substrate.

    摘要翻译： 将膜脂质施用于基材的方法包括提供基材和具有包含膜脂质的油墨的油墨储存器。 将扫描探针显微镜的尖端浸入墨水中，以将膜脂质置于尖端上。 使扫描探针显微镜的尖端与基板的表面接触。 尖端在表面的区域上移动，使得膜脂质从扫描探针显微镜的尖端迁移到区域中的基底表面上，并且膜脂质在单个脂质层形式的区域中组织其自身或 以一个或多个相互重叠的脂双层的形式。 尖端从衬底的表面移除。

    Massively parallel lithography with two-dimensional pen arrays
    9.
    发明授权
    Massively parallel lithography with two-dimensional pen arrays 失效
    大尺寸平行光刻与二维笔阵列

    公开(公告)号：US08192794B2

    公开(公告)日：2012-06-05

    申请号：US12000456

    申请日：2007-12-12

    申请人： Chad A. Mirkin ,  Peng Sun ,  Yuhuang Wang ,  Steven Lenhert

    发明人： Chad A. Mirkin ,  Peng Sun ,  Yuhuang Wang ,  Steven Lenhert

    IPC分类号： B05D5/00 ,  G01N1/28

    CPC分类号： G03F7/0002 ,  G01Q80/00

    摘要： Massive parallel printing of structures and nanostructures, including lipids, at high speed with high resolution and high quality using two dimensional arrays comprising cantilevers and tip-based transfer of material to a surface. The invention provides a nanolithographic method comprising (1) providing a two-dimensional array of a plurality of cantilevers, wherein the array comprises a plurality of base rows, each base row comprising a plurality of cantilevers extending from the base row, wherein each of the cantilevers comprising tips at the cantilever end away from the base row; wherein the two dimensional array has a support; (2) providing a patterning composition, wherein the composition comprises one or more lipids; (3) providing a substrate; (4) coating the tips of the cantilevers with the patterning composition; and (5) depositing at least some of the patterning composition from the tips to the substrate surface.

    摘要翻译： 使用包括悬臂和基于尖端的材料转移到表面的二维阵列，以高分辨率和高质量的高速大规模平行印刷结构和纳米结构，包括脂质。 本发明提供一种纳米光刻方法，其包括（1）提供多个悬臂的二维阵列，其中所述阵列包括多个基列，每个基行包括从所述基列延伸的多个悬臂，其中， 悬臂包括远离基座的悬臂端的尖端; 其中所述二维阵列具有支撑; （2）提供图案化组合物，其中所述组合物包含一种或多种脂质; （3）提供基板; （4）用图案化组合物涂覆悬臂的尖端; 和（5）将至少一些图案化组合物从尖端沉积到​​基底表面。