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    Method and apparatus for variable-field illumination
    1.
    发明申请
    Method and apparatus for variable-field illumination 审中-公开
    用于可变场照明的方法和装置

    公开(公告)号：US20060170916A1

    公开(公告)日：2006-08-03

    申请号：US11045051

    申请日：2005-01-31

    申请人： Thomas Voigt ,  David Tuschel ,  Jingyun Zhang ,  Patrick Treado

    发明人： Thomas Voigt ,  David Tuschel ,  Jingyun Zhang ,  Patrick Treado

    IPC分类号： G01J3/44 ,  G01N21/65

    CPC分类号： G01J3/44 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0224 ,  G01N21/65

    摘要： The disclosure relates to identifying one or more regions of interest within a broader field of view of a dynamic sample using one or more optical components and illuminating photons. Once the region of interest is identified within a section of the broader field of view, chemical information in the form of Raman spectrum is obtained from the region of interest by focusing the illuminating photons or the optical components on the region of interest.

    摘要翻译： 本公开涉及使用一个或多个光学组件和照射光子来识别动态样本的更宽视野内的一个或多个感兴趣区域。 一旦在较宽视场的一部分内识别出感兴趣区域，则通过将照射光子或光学部件聚焦在感兴趣区域上，从感兴趣的区域获得拉曼光谱形式的化学信息。

    Method and apparatus for a microscope image selector
    2.
    发明申请
    Method and apparatus for a microscope image selector 有权
    显微镜图像选择器的方法和装置

    公开(公告)号：US20060170922A1

    公开(公告)日：2006-08-03

    申请号：US11045080

    申请日：2005-01-31

    申请人： Xinghua Wang ,  Thomas Voigt ,  David Tuschel ,  Chenhui Wang ,  Patrick Treado

    发明人： Xinghua Wang ,  Thomas Voigt ,  David Tuschel ,  Chenhui Wang ,  Patrick Treado

    IPC分类号： G01N21/64 ,  G01N21/65

    CPC分类号： G01N21/65 ,  G01J3/02 ,  G01J3/0291 ,  G01J3/36 ,  G01J3/44 ,  G01N21/359 ,  G01N21/59 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/656

    摘要： The disclosure generally relates to a multimode imaging apparatus for simultaneously obtaining multiple wavelength-discriminative spectral images of a sample. In one embodiment, the apparatus includes an image selector having a rotator assembly, the rotator assembly housing a first plurality of optical components, the image selector adapted to receive a illuminating photons having a first wavelength and direct the illuminating photons to the sample, the image selector adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; a microscope turret housing a second plurality of components, the microscope turret adapted to receive illuminating photons having a second wavelength and direct the photons to the sample; the microscopic turret adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; wherein substantially all of the interacted photons are selectively directed one of a plurality of detection sources to form multiple wavelength discriminative spectral images of the sample simultaneously.

    摘要翻译： 本公开总体上涉及用于同时获得样本的多个波长鉴别光谱图像的多模式成像装置。 在一个实施例中，该装置包括具有旋转器组件的图像选择器，旋转体组件容纳第一多个光学部件，图像选择器适于接收具有第一波长的照明光子并将照射光子引导到样本，图像 选择器，适于接收与样本相互作用的照明光子，并选择性地将所述相互作用的光子引导到多个检测源之一; 显微镜转台，其容纳第二多个部件，所述显微镜转台适于接收具有第二波长并且将所述光子引导到所述样品的照明光子; 适于接收与样品相互作用的照明光子并且选择性地将所述相互作用的光子引导到多个检测源中的一个的微观炮塔; 其中基本上所有相互作用的光子被选择性地指向多个检测源中的一个，以同时形成样品的多个波长鉴别光谱图像。

    Method and apparatus for a microscope image selector
    3.
    发明授权
    Method and apparatus for a microscope image selector 有权
    显微镜图像选择器的方法和装置

    公开(公告)号：US07414725B2

    公开(公告)日：2008-08-19

    申请号：US11902874

    申请日：2007-09-26

    申请人： Xinghua Wang ,  Thomas Voigt ,  David Tuschel ,  Chenhui Wang ,  Patrick Treado

    发明人： Xinghua Wang ,  Thomas Voigt ,  David Tuschel ,  Chenhui Wang ,  Patrick Treado

    IPC分类号： G01N21/25 ,  G02B21/00

    CPC分类号： G01N21/65 ,  G01J3/02 ,  G01J3/0291 ,  G01J3/36 ,  G01J3/44 ,  G01N21/359 ,  G01N21/59 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/656

    摘要： The disclosure generally relates to a multimode imaging apparatus for simultaneously obtaining multiple wavelength-discriminative spectral images of a sample. In one embodiment, the apparatus includes an image selector having a rotator assembly, the rotator assembly housing a first plurality of optical components, the image selector adapted to receive a illuminating photons having a first wavelength and direct the illuminating photons to the sample, the image selector adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; a microscope turret housing a second plurality of components, the microscope turret adapted to receive illuminating photons having a second wavelength and direct the photons to the sample; the microscopic turret adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; wherein substantially all of the interacted photons are selectively directed one of a plurality of detection sources to form multiple wavelength discriminative spectral images of the sample simultaneously.

    摘要翻译： 本公开总体上涉及用于同时获得样本的多个波长鉴别光谱图像的多模式成像装置。 在一个实施例中，该装置包括具有旋转器组件的图像选择器，旋转体组件容纳第一多个光学部件，图像选择器适于接收具有第一波长的照明光子并将照射光子引导到样本，图像 选择器，适于接收与样本相互作用的照明光子，并选择性地将所述相互作用的光子引导到多个检测源之一; 显微镜转台，其容纳第二多个部件，所述显微镜转台适于接收具有第二波长并且将所述光子引导到所述样品的照明光子; 适于接收与样品相互作用的照明光子并且选择性地将所述相互作用的光子引导到多个检测源中的一个的微观炮塔; 其中基本上所有相互作用的光子被选择性地指向多个检测源中的一个，以同时形成样品的多个波长鉴别光谱图像。

    Method and apparatus for a microscope image selector
    4.
    发明申请
    Method and apparatus for a microscope image selector 有权

    公开(公告)号：US20080088844A1

    公开(公告)日：2008-04-17

    申请号：US11902874

    申请日：2007-09-26

    申请人： Xinghua Wang ,  Thomas Voigt ,  David Tuschel ,  Chenhui Wang ,  Patrick Treado

    发明人： Xinghua Wang ,  Thomas Voigt ,  David Tuschel ,  Chenhui Wang ,  Patrick Treado

    IPC分类号： G01N21/25

    CPC分类号： G01N21/65 ,  G01J3/02 ,  G01J3/0291 ,  G01J3/36 ,  G01J3/44 ,  G01N21/359 ,  G01N21/59 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/656

    摘要： The disclosure generally relates to a multimode imaging apparatus for simultaneously obtaining multiple wavelength-discriminative spectral images of a sample. In one embodiment, the apparatus includes an image selector having a rotator assembly, the rotator assembly housing a first plurality of optical components, the image selector adapted to receive a illuminating photons having a first wavelength and direct the illuminating photons to the sample, the image selector adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; a microscope turret housing a second plurality of components, the microscope turret adapted to receive illuminating photons having a second wavelength and direct the photons to the sample; the microscopic turret adapted to receive illuminating photons interacted with the sample and selectively direct said interacted photons to one of a plurality of detection sources; wherein substantially all of the interacted photons are selectively directed one of a plurality of detection sources to form multiple wavelength discriminative spectral images of the sample simultaneously.

    Dynamic imaging of biological cells and other subjects
    5.
    发明申请
    Dynamic imaging of biological cells and other subjects 有权
    生物细胞和其他科目的动态成像

    公开(公告)号：US20070182959A1

    公开(公告)日：2007-08-09

    申请号：US10556038

    申请日：2005-06-30

    申请人： John Maier ,  Patrick Treado ,  David Tuschel ,  Thomas Voigt

    发明人： John Maier ,  Patrick Treado ,  David Tuschel ,  Thomas Voigt

    IPC分类号： G01J3/44 ,  G01N21/65

    CPC分类号： G01N21/6456 ,  G01J3/2803 ,  G01J3/32 ,  G01J3/36 ,  G01J3/44 ,  G01N21/359 ,  G01N21/6486 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/656

    摘要： The invention relates to methods of dynamic chemical imaging, including methods of cellular imaging. The method comprises illuminating at least a portion of a cell with substantially monochromatic light and assessing Raman-shifted light scattered from the illuminated portion at a plurality of discrete times. The Raman-shifted light can be assessed at a plurality of Raman shift (RS) values at each of the discrete times, and the RS values can be selected to be characteristic of a pre-selected component at each of the discrete times. Multivariate analysis of Raman spectral features of the images thus obtained can yield the location and chemical identity of components in the field of view. This information can be combined or overlaid with other spectral data (e.g., a visible microscopic image) obtained from the field of view.

    摘要翻译： 本发明涉及动态化学成像方法，包括细胞成像方法。 该方法包括以基本上单色的光照亮电池的至少一部分，并且在多个离散时间评估从照射部分散射的拉曼偏移光。 可以在每个离散时间处以多个拉曼位移（RS）值评估拉曼移位的光，并且可以将每个离散时间的RS值选择为预选择的分量的特征。 如此获得的图像的拉曼光谱特征的多变量分析可以产生视场中部件的位置和化学特性。 该信息可以与从视野获得的其他光谱数据（例如，可见显微镜图像）组合或覆盖。

    System and method to perform raman imaging without luminescence
    6.
    发明授权
    System and method to perform raman imaging without luminescence 有权
    不发光的拉曼成像系统和方法

    公开(公告)号：US07649618B2

    公开(公告)日：2010-01-19

    申请号：US11728430

    申请日：2007-03-26

    申请人： David Tuschel ,  Jingyun Zhang

    发明人： David Tuschel ,  Jingyun Zhang

    IPC分类号： G01J3/44 ,  G01N21/64

    CPC分类号： G01J3/44 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/0294 ,  G01J3/2803 ,  G01J3/2889 ,  G01J2003/4424 ,  G01N21/6456 ,  G01N21/65 ,  G01N2021/6417 ,  G01N2021/6423

    摘要： A system and method for collecting Raman data sets without the “contaminating” effect of luminescence emitted photons. Using a frame transfer CCD for time resolved data collection, Raman imaging may be performed without photobleaching the sample. The system may include a light source, a frame transfer CCD, an optical lens and at least one controller. The light source illuminates the sample with a plurality of photons to generate scattered photons from the sample. The frame transfer CCD has an image array and a storage array. The optical lens collects scattered photons and directs the scattered photons to the image array. The controller transfers a Raman data set representative of the scattered photons from the image array to the storage array. The frame transfer CCD may be configured so as the image array integrates the scattered photons during a Raman integration time and the controller transfers the Raman data set from the image array to storage array during a parallel transfer time. The sum of the Raman integration time and the parallel transfer time is less than the minimum time it takes to trigger luminescence from the sample.

    摘要翻译： 用于收集拉曼数据集而不发出发光光子的“污染”影响的系统和方法。 使用帧传输CCD进行时间分辨数据采集，拉曼成像可以在没有光漂白样品的情况下进行。 该系统可以包括光源，帧传送CCD，光学透镜和至少一个控制器。 光源用多个光子照射样品以从样品产生散射的光子。 帧传送CCD具有图像阵列和存储阵列。 光学透镜收集散射的光子并将散射的光子引导到图像阵列。 控制器将表示散射光子的拉曼数据集从图像阵列传送到存储阵列。 帧传送CCD可以被配置为使得图像阵列在拉曼积分时间期间对散射的光子进行积分，并且控制器在平行传送时间期间将拉曼数据集从图像阵列传送到存储阵列。 拉曼积分时间和平行传输时间的总和小于从样品触发发光所需的最小时间。

    System and method to perform raman imaging without luminescence
    7.
    发明申请
    System and method to perform raman imaging without luminescence 有权
    不发光的拉曼成像系统和方法

    公开(公告)号：US20070222982A1

    公开(公告)日：2007-09-27

    申请号：US11728430

    申请日：2007-03-26

    申请人： David Tuschel ,  Jingyun Zhang

    发明人： David Tuschel ,  Jingyun Zhang

    IPC分类号： G01J3/44 ,  G01N21/65

    CPC分类号： G01J3/44 ,  G01J3/02 ,  G01J3/0218 ,  G01J3/0294 ,  G01J3/2803 ,  G01J3/2889 ,  G01J2003/4424 ,  G01N21/6456 ,  G01N21/65 ,  G01N2021/6417 ,  G01N2021/6423

    摘要： A system and method for collecting Raman data sets without the “contaminating” effect of luminescence emitted photons. Using a frame transfer CCD for time resolved data collection, Raman imaging may be performed without photobleaching the sample. The system may include a light source, a frame transfer CCD, an optical lens and at least one controller. The light source illuminates the sample with a plurality of photons to generate scattered photons from the sample. The frame transfer CCD has an image array and a storage array. The optical lens collects scattered photons and directs the scattered photons to the image array. The controller transfers a Raman data set representative of the scattered photons from the image array to the storage array. The frame transfer CCD may be configured so as the image array integrates the scattered photons during a Raman integration time and the controller transfers the Raman data set from the image array to storage array during a parallel transfer time. The sum of the Raman integration time and the parallel transfer time is less than the minimum time it takes to trigger luminescence from the sample.

    摘要翻译： 用于收集拉曼数据集而不发出发光光子的“污染”影响的系统和方法。 使用帧传输CCD进行时间分辨数据采集，拉曼成像可以在没有光漂白样品的情况下进行。 该系统可以包括光源，帧传送CCD，光学透镜和至少一个控制器。 光源用多个光子照射样品以从样品产生散射的光子。 帧传送CCD具有图像阵列和存储阵列。 光学透镜收集散射的光子并将散射的光子引导到图像阵列。 控制器将表示散射光子的拉曼数据集从图像阵列传送到存储阵列。 帧传送CCD可以被配置为使得图像阵列在拉曼积分时间期间对散射的光子进行积分，并且控制器在平行传送时间期间将拉曼数据集从图像阵列传送到存储阵列。 拉曼积分时间和平行传输时间的总和小于从样品触发发光所需的最小时间。

    Method and applications to enhance and image optical signals from biological objects
    9.
    发明申请
    Method and applications to enhance and image optical signals from biological objects 审中-公开
    从生物对象增强和成像光信号的方法和应用

    公开(公告)号：US20060250613A1

    公开(公告)日：2006-11-09

    申请号：US11404964

    申请日：2006-04-14

    申请人： Joseph Demuth ,  Patrick Treado ,  John Maier ,  David Tuschel

    发明人： Joseph Demuth ,  Patrick Treado ,  John Maier ,  David Tuschel

    IPC分类号： G01J3/44 ,  G01N21/65

    CPC分类号： G01J3/44 ,  G01J3/32 ,  G01N21/658

    摘要： A method and apparatus for imaging biological objects. A SERS surface is provided having enhancing structures uniformly distributed on the surface. The surface includes a two dimensional area of at least 5×105 nm. The enhancing structures may have a size, in at least one dimension of height, width and length, ranging from 100 nm to 1000 nm. A biological material is deposited on the SERS surface. The biological material on the SERS surface is illuminated using a monochromatic light source producing Raman scattered photons. The Raman scattered photons are filtered using a tunable filter into a plurality of predetermined wavelength bands. A two-dimensional array detector detects the filtered Raman scattered photons, in a spatially accurate manner. The results of filtering and detecting steps are combined to produce a plurality of spectrally resolved Raman images of the biological material.

    摘要翻译： 一种用于成像生物体的方法和装置。 提供了具有均匀分布在表面上的增强结构的SERS表面。 该表面包括至少5×10 5 nm的二维区域。 增强结构可以在100nm至1000nm范围内的高度，宽度和长度的至少一个尺寸中具有尺寸。 生物材料沉积在SERS表面上。 使用产生拉曼散射光子的单色光源照射SERS表面上的生物材料。 使用可调滤波器将拉曼散射光子滤波成多个预定波长带。 二维阵列检测器以空间准确的方式检测经过滤波的拉曼散射光子。 滤波和检测步骤的结果被组合以产生生物材料的多个光谱分辨的拉曼图像。