公开(公告)号：US4842997A

公开(公告)日：1989-06-27

申请号：US551985

申请日：1983-10-27

申请人： Timothy J. N. Carter ,  Carol J. Groucutt ,  Richard A. W. Stott ,  Gary H. G. H. Thorpe ,  Thomas P. Whitehead

发明人： Timothy J. N. Carter ,  Carol J. Groucutt ,  Richard A. W. Stott ,  Gary H. G. H. Thorpe ,  Thomas P. Whitehead

IPC分类号： G01N21/76 ,  C12Q1/28 ,  G01N33/58

CPC分类号： C12Q1/28 ,  G01N33/581 ,  Y10S435/81 ,  Y10S435/968

摘要： Assays, e.g. immunoassays, based on the chemiluminescent reaction between a peroxidase enzyme, e.g. horse radish peroxidase, a chemiluminescent 2,3-dihydro-1,4-phthalazinedione, e.g. luminol or isoluminol and an oxidant, e.g. hydrogen peroxide or a perborate, are improved by carrying out the reaction in the presence of a 6-hydroxybenzothiazole, especially 6-hydroxybenzothiazole itself or firefly luciferin. Light emission from the reaction is significantly enhanced by the 6-hydroxybenzothiazole.

摘要翻译： PCT No.PCT / GB83 / 00058 Sec。 371日期1983年10月27日第 102（e）日期1983年10月27日PCT提交1983年2月25日PCT公布。 公开号WO83 / 03104 日期：1983年9月15日。 基于过氧化物酶之间的化学发光反应的免疫测定。 辣根过氧化物酶，化学发光2,3-二氢-1,4-酞嗪二酮，例如 鲁米诺或异鲁米诺和氧化剂，例如 过氧化氢或过硼酸盐，通过在6-羟基苯并噻唑，特别是6-羟基苯并噻唑本身或萤火虫荧光素的存在下进行反应得到改善。 由6-羟基苯并噻唑显着增强反应的光发射。

公开(公告)号：US4366118A

公开(公告)日：1982-12-28

申请号：US189930

申请日：1980-02-14

申请人： Roger A. Bunce ,  Timothy J. N. Carter ,  John H. Kennedy ,  Larry J. Kricka ,  Thomas P. Whitehead

发明人： Roger A. Bunce ,  Timothy J. N. Carter ,  John H. Kennedy ,  Larry J. Kricka ,  Thomas P. Whitehead

IPC分类号： G01N33/58 ,  G01N21/01 ,  G01N21/76

CPC分类号： G01N21/76

摘要： The apparatus comprises a movable member (10) which carries a plurality of cuvettes (11) containing material under test. Control means cause the member (10) to be moved to bring the cuvettes (11) successively into register with a photomultiplier (16), and in that position reagent is automatically added to a cuvette, giving rise to a luminescent reaction. Light from the reaction is detected by the photo-multiplier (16) and the resultant signal processed and recorded. The control means is triggered to stop the member (10), add reagent, record the signal from the luminescent reaction, and restart the member, from a photocell (28) which is illuminated through the member (10) only when a cuvette (11) is in register with the photomultiplier (16).According to the method, sample material containing the analyte for determination is mixed with a known amount or concentration of the same analyte labelled with a substance which is capable of taking part in a luminescent reaction. The mixture is then reacted with a known amount of another substance capable of reacting with the analyte and giving rise to a luminescent reaction with the labelling substance. From quantitative observation of the light from the luminescent reaction the amount or concentration of the analyte in the sample may be deduced.

摘要翻译： PCT No.PCT / GB79 / 00102 Sec。 371日期1980年2月14日 102（e）1980年2月14日PCT PCT。 出版物WO80 / 00100 PCT 日期为1980年1月24日。该装置包括可移动构件（10），其携带包含被测材料的多个比色皿（11）。 控制装置使得构件（10）移动以使比色杯（11）依次与光电倍增器（16）对准，并且在该位置，试剂自动添加到比色杯中，引起发光反应。 通过光电倍增器（16）检测反应的光，并对所得信号进行处理和记录。 控制装置被触发以停止构件（10），添加试剂，记录来自发光反应的信号，并从仅通过构件（10）照射的光电池（28）重新启动构件，只有当比色杯（11 ）与光电倍增管（16）对齐。 根据该方法，将含有用于测定的分析物的样品材料与能够参与发光反应的物质标记的相同分析物的已知量或浓度混合。 然后将混合物与已知量的能够与分析物反应并产生与标记物质的发光反应的另一种物质反应。 通过定量观察发光反应的光，可以推导出样品中分析物的量或浓度。

公开(公告)号：US5035508A

公开(公告)日：1991-07-30

申请号：US139181

申请日：1987-12-29

申请人： Timothy J. N. Carter ,  Roger A. Bunce

发明人： Timothy J. N. Carter ,  Roger A. Bunce

IPC分类号： G01N21/27 ,  A61B5/00 ,  G01J1/16 ,  G01J3/12 ,  G01N21/25 ,  G02B6/00

CPC分类号： A61B5/0059 ,  G01N21/255 ,  G01J1/1626 ,  G01J2003/1226 ,  G01N2021/1742 ,  G01N2201/0696 ,  G01N2201/084

摘要： A light absorption analyzer can work in either a reflection or a transmission mode, according to which probe head is fitted to it.Light is caused to be of a desired wavelength for the analysis by passing non-monochromatic light from a high-intensity flash tube source (which is not a point source) to an interference filter. Only parallel light emanating from the filter is focused by a concave parabolic mirror on a point where at least one fibre-optic collector is positioned. This light can then be passed directly to the probe for passage through the sample, since it is only light of a specified wavelength which will have travelled parallel from the filter and will therefore be focused at the point. Some of the light is taken off a reference detector to provide a reference signal for comparison with that derived from a test detetor fed by a return fibre-optic from the probe. A comparator is programmed to give a quantitiative readout of the absorption by the test sample.

公开(公告)号：USRE33064E

公开(公告)日：1989-09-19

申请号：US107763

申请日：1987-10-09

申请人： Timothy J. N. Carter ,  Claus Dahne ,  John F. Place

发明人： Timothy J. N. Carter ,  Claus Dahne ,  John F. Place

IPC分类号： G01N21/77 ,  G01N33/543

CPC分类号： G01N33/54373 ,  G01N21/7703

摘要： An analyte in solution is made to react with a specific reactant coated on the wave-guide thus modifying the optical properties thereof. The index of refraction of the wave-guide material is higher than that of the reaction medium which ensures that a light signal injected into said guide be carried by multiple total reflection, the distance of penetration of the evanescent wave associated with the totally reflected signal being of the same order of magnitude or greater than the thickness of the analyte-reactant product layer.

公开(公告)号：US4608344A

公开(公告)日：1986-08-26

申请号：US499164

申请日：1983-05-18

申请人： Timothy J. N. Carter ,  Claus Dahne ,  John F. Place

发明人： Timothy J. N. Carter ,  Claus Dahne ,  John F. Place

IPC分类号： G01N21/64 ,  G01N21/27 ,  G01N21/77 ,  G01N33/543 ,  G01N33/553 ,  G01N33/48

CPC分类号： G01N21/648 ,  G01N21/6452 ,  G01N21/7703 ,  G01N33/553 ,  Y10S436/805

摘要： An analyte in solution is made to react with a spectific reactant coated on the wave-guide thus modifying the optical properties thereof. The index of refraction of the wave-guide material is higher than that of the reaction medium which ensures that a light signal injected into said guide be carried by multiple total reflection, the distance of penetration of the evanescent wave associated with the totally reflected signal being of the same order of magnitude or greater than the thickness of the analyte-reactant product layer.

摘要翻译： PCT No.PCT / EP82 / 00195 Sec。 371日期1983年5月18日 102（e）日期1983年5月18日PCT提交1982年9月8日PCT公布。 出版物WO83 / 01112 PCT 日期为1983年3月31日。使溶液中的分析物与涂覆在波导上的光谱反应物反应，从而改变其光学性质。 波导材料的折射率高于反应介质的折射率，其确保注入到导向器中的光信号通过多次全反射承载，与全反射信号相关联的ev逝波的穿透距离为 具有相同数量级或大于分析物 - 反应物产物层的厚度。

公开(公告)号：US4508832A

公开(公告)日：1985-04-02

申请号：US389973

申请日：1982-06-18

申请人： Timothy J. N. Carter ,  Claus Dahne

发明人： Timothy J. N. Carter ,  Claus Dahne

IPC分类号： G01N21/21 ,  G01N21/77 ,  G01N33/543 ,  G01N33/54 ,  G01N21/17

CPC分类号： G01N21/552 ,  G01N21/211 ,  G01N33/54373 ,  Y10S436/805

摘要： A layer of bioactive molecules is coated on a dielectric substrate and is contacted with a solution to be analyzed containing a complex conjugate of said molecule. The rate of complexion of said conjugate moiety with the layer which is a function of its concentration in the analyte is measured by optical means and correlated with corresponding rates obtained from standard reference measurements, thus providing data for determining the unknown concentration of said conjugate moiety in the solution to be analyzed.

摘要翻译： 将一层生物活性分子涂覆在电介质底物上并与待分析的溶液接触，所述溶液含有所述分子的复合缀合物。 通过光学方法测量所述缀合物部分与被分析物中其浓度的函数关系的层的比例，并与从标准参考测量获得的相应速率相关联，从而提供用于确定所述缀合物部分的未知浓度的数据 要分析的解决方案。