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    STORAGE-STABLE LIQUID WASHING OR CLEANING AGENT CONTAINING PROTEASE AND AMYLASE
    2.
    发明申请
    STORAGE-STABLE LIQUID WASHING OR CLEANING AGENT CONTAINING PROTEASE AND AMYLASE 审中-公开
    储存稳定的液体清洗剂或清洁剂，含有蛋白酶和淀粉酶

    公开(公告)号：US20140018282A1

    公开(公告)日：2014-01-16

    申请号：US13993898

    申请日：2011-12-13

    申请人： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell ,  Hendrik Hellmuth

    发明人： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell ,  Hendrik Hellmuth

    IPC分类号： C11D3/386

    摘要： According to the invention, storage stability in terms of amylolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and amylase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.

    摘要翻译： 根据本发明，在包含蛋白酶和淀粉酶的液体洗涤剂或清洁剂中，淀粉分解活性的储存稳定性得到改善。 这通过使用蛋白酶来实现，所述蛋白酶包含与SEQ ID NO：1中规定的氨基酸序列至少80％相同的氨基酸序列。 1，其具有氨基酸谷氨酸（E）或天冬氨酸（D）或氨基酸天冬酰胺（N）或谷氨酰胺（Q）或氨基酸丙氨酸（A）或甘氨酸（G）或丝氨酸（S） 根据SEQ ID NO。 1。

    STORAGE-STABLE LIQUID WASHING OR CLEANING AGENT CONTAINING PROTEASE AND CELLULASE
    3.
    发明申请
    STORAGE-STABLE LIQUID WASHING OR CLEANING AGENT CONTAINING PROTEASE AND CELLULASE 审中-公开
    储存稳定的液体清洗剂或清洁剂，含有蛋白质和细胞因子

    公开(公告)号：US20130280794A1

    公开(公告)日：2013-10-24

    申请号：US13993923

    申请日：2011-12-13

    申请人： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell ,  Hendrik Hellmuth

    发明人： Susanne Wieland ,  Karl-Heinz Maurer ,  Timothy O'Connell ,  Hendrik Hellmuth

    IPC分类号： C11D3/386

    CPC分类号： C11D3/38663 ,  C11D3/38618 ,  C11D3/38645 ,  C12N9/2437 ,  C12N9/54

    摘要： According to the invention, storage stability in terms of cellulolytic activity is to be improved in a liquid washing or cleaning agent which comprises a protease and cellulase. This is achieved by the use of a protease which comprises an amino acid sequence which is at least 80% identical to the amino acid sequence specified in SEQ ID NO. 1 and which has the amino acid glutamic acid (E) or aspartic acid (D) or the amino acid asparagine (N) or glutamine (Q) or the amino acid alanine (A) or glycine (G) or serine (S) at position 99 in the count according to SEQ ID NO. 1.

    摘要翻译： 根据本发明，在包含蛋白酶和纤维素酶的液体洗涤剂或清洁剂中，纤维素分解活性的储存稳定性得到改善。 这通过使用蛋白酶来实现，所述蛋白酶包含与SEQ ID NO：1中规定的氨基酸序列至少80％相同的氨基酸序列。 1，其具有氨基酸谷氨酸（E）或天冬氨酸（D）或氨基酸天冬酰胺（N）或谷氨酰胺（Q）或氨基酸丙氨酸（A）或甘氨酸（G）或丝氨酸（S） 根据SEQ ID NO。 1。

    Alpha-amylase variants stabilized against dimerization and/or multimerization, method for the production thereof, and detergents and cleansers containing these alpha-amylase variants
    5.
    发明授权
    Alpha-amylase variants stabilized against dimerization and/or multimerization, method for the production thereof, and detergents and cleansers containing these alpha-amylase variants 有权
    稳定抗二聚和/或多聚化的α-淀粉酶变体，其生产方法，以及含有这些α-淀粉酶变体的洗涤剂和清洁剂

    公开(公告)号：US08785365B2

    公开(公告)日：2014-07-22

    申请号：US11692691

    申请日：2007-03-28

    申请人： Cornelius Bessler ,  Susanne Wieland ,  Karl-Heinz Maurer

    发明人： Cornelius Bessler ,  Susanne Wieland ,  Karl-Heinz Maurer

    IPC分类号： C12N9/28 ,  C11D3/386 ,  C12S11/00 ,  C12S9/00

    CPC分类号： C12N9/2417 ,  C11D3/38681

    摘要： The present invention relates to α-amylase variants that are stabilized to dimerization and/or multimerization, in particular at elevated temperatures or high pH, by point mutagenesis of positively polarized or charged or neutral surface amino acids to give more negatively polarized or charged amino acids. The invention further relates to methods of increasing the stability of an α-amylase to dimerization and/or multimerization brought about by electrostatic interactions whereby at least one amino acid residue on the surface of the starting molecule, which makes a neutral or positively polar or charged contribution to the electrostatic potential of said molecule, is replaced with a more negatively polar or negatively charged amino acid residue. The α-amylase variants obtained thereby exhibit better stability to influences of the solvent, increased processivity, and are suited for numerous industrial areas of use, in particular as active ingredients in detergents and cleansers.

    摘要翻译： 本发明涉及通过正极化或带电荷或中性表面氨基酸的点诱变稳定至二聚化和/或多聚化的α-淀粉酶变体，特别是在升高的温度或高pH下，以产生更多的负极化或带电荷的氨基酸 。 本发明还涉及增加α-淀粉酶对由静电相互作用引起的二聚化和/或多聚化的稳定性的方法，其中起始分子表面上至少有一个氨基酸残基，其形成中性或正极性或带电 对所述分子的静电电位的贡献被更负极性或带负电荷的氨基酸残基取代。 由此获得的α-淀粉酶变体对溶剂的影响表现出更好的稳定性，增加的持续性，并且适用于许多工业使用领域，特别是作为洗涤剂和清洁剂中的活性成分。

    Novel alkaline protease and washing and cleaning products containing said novel alkaline protease
    7.
    发明申请
    Novel alkaline protease and washing and cleaning products containing said novel alkaline protease 有权
    新型碱性蛋白酶和含有所述新型碱性蛋白酶的洗涤和清洁产品

    公开(公告)号：US20070010417A1

    公开(公告)日：2007-01-11

    申请号：US11473708

    申请日：2006-06-22

    申请人： Susanne Wieland ,  Karl-Heinz Maurer ,  Beatrix Kottwitz ,  Frank Niehaus ,  Patrick Lorenz

    发明人： Susanne Wieland ,  Karl-Heinz Maurer ,  Beatrix Kottwitz ,  Frank Niehaus ,  Patrick Lorenz

    IPC分类号： C11D3/386 ,  C07H21/04 ,  C12P21/06 ,  C12N9/54 ,  C12N1/21

    CPC分类号： C11D3/386 ,  C12N9/50

    摘要： The invention relates to a novel alkaline protease, the DNA thereof having been taken from a bottom sample, to all alkaline proteases that are at least 40% identical, and to all nucleic acids with an identity at least 50% homologous with the associated nucleic acids. The invention also relates to the fragment of said protease, comprising the amino acid positions 108 to 325, in addition to the gene fragment coding therefor, and to all alkaline proteases that are at least 60% identical, or nucleic acids that are at least 70% identical. Furthermore, the invention defines technical possibilities of use for said proteases and especially describes the use thereof in washing and cleaning products.

    摘要翻译： 本发明涉及一种新的碱性蛋白酶，其DNA从底部样品中获取至所有与至少40％相同的碱性蛋白酶，以及所有与相关核酸具有至少50％同源性的核酸 。 本发明还涉及所述蛋白酶的片段，其除了编码该基因片段的氨基酸位置108至325之外，还涉及至少60％相同的所有碱性蛋白酶，或至少70个核酸 ％相同。 此外，本发明定义了用于所述蛋白酶的技术可能性，并且特别描述了在洗涤和清洁产品中的用途。

    Alpha-amylase variants having an elevated solvent stability, method for the production thereof and detergents and cleansers containing these alpha-amylase variants
    10.
    发明授权
    Alpha-amylase variants having an elevated solvent stability, method for the production thereof and detergents and cleansers containing these alpha-amylase variants 有权
    具有提高的溶剂稳定性的α-淀粉酶变体，其制备方法和含有这些α-淀粉酶变体的洗涤剂和清洁剂

    公开(公告)号：US08080401B2

    公开(公告)日：2011-12-20

    申请号：US11692504

    申请日：2007-03-28

    申请人： Cornelius Bessler ,  Susanne Wieland ,  Karl-Heinz Maurer

    发明人： Cornelius Bessler ,  Susanne Wieland ,  Karl-Heinz Maurer

    IPC分类号： C12N9/28 ,  C12N9/26 ,  C12N9/00 ,  C02F3/34 ,  C11D3/00 ,  C07H21/04 ,  C07K14/00

    CPC分类号： C11D3/38681 ,  C12N9/2417

    摘要： The present invention relates to α-amylase variants that are stabilized to solvent-exerted hydrolysis, in particular at elevated temperatures or high pH, by point mutagenesis of asparagine (N) or glutamine (Q) residues located on the surface of the molecule to give other amino acid residues. The invention further relates to methods of increasing the stability of an α-amylase to solvent-exerted hydrolysis, in particular at elevated temperatures or high pH, whereby at least one asparagine (N) or glutamine (Q) residue on the surface of the molecule is replaced with a different amino acid residue. The α-amylase variants obtained thereby exhibit better stability to influences of the solvent, increased processivity, and are suited for numerous industrial areas of use, in particular as active ingredients in detergents and cleansers.

    摘要翻译： 本发明涉及通过位于分子表面上的天冬酰胺（N）或谷氨酰胺（Q）残基的点诱变而稳定化为溶剂施加水解的α-淀粉酶变体，特别是在升高的温度或高pH下，得到 其他氨基酸残基。 本发明还涉及增加α-淀粉酶对溶剂发生水解的稳定性的方法，特别是在升高的温度或高pH下，其中分子表面上至少有一个天冬酰胺（N）或谷氨酰胺（Q）残基 被不同的氨基酸残基取代。 由此获得的α-淀粉酶变体对溶剂的影响表现出更好的稳定性，增加的持续性，并且适用于许多工业使用领域，特别是作为洗涤剂和清洁剂中的活性成分。