Buffer Composition for Hybridization Use, and Hybridization Method
    2.
    发明申请
    Buffer Composition for Hybridization Use, and Hybridization Method 审中-公开
    用于杂交用途的缓冲液组合物和杂交方法

    公开(公告)号:US20160265037A1

    公开(公告)日:2016-09-15

    申请号:US15024392

    申请日:2014-09-01

    IPC分类号: C12Q1/68

    摘要: A buffer composition for hybridization of a target nucleic acid is provided. The nucleic acid can include a nucleotide to be detected with a nucleic acid probe. The probe can contain a nucleotide sequence complementary to the target nucleic acid. The buffer can include a blocking nucleic acid having a nucleotide sequence complementary to a non-target nucleic acid having a nucleotide not to be detected corresponding to the nucleotide to be detected. The buffer composition can suppress non-specific hybridization to the nucleic acid probe even when a non-target nucleic acid is present. The use of the buffer composition can achieve excellent detection efficiency of the target nucleic acid.

    摘要翻译: 提供了用于靶核酸杂交的缓冲组合物。 核酸可以包括用核酸探针检测的核苷酸。 探针可以含有与靶核酸互补的核苷酸序列。 缓冲液可以包括具有与非靶核酸互补的核苷酸序列的阻断核酸,其具有对应于待检测核苷酸的不被检测的核苷酸。 即使存在非靶核酸,缓冲组合物也可以抑制与核酸探针的非特异性杂交。 使用缓冲组合物可以实现目标核酸的优异检测效率。