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公开(公告)号：US20160265037A1
公开(公告)日：2016-09-15
申请号：US15024392
申请日：2014-09-01
发明人： Shuichi Kamei , Mayuko Hosoya , Masaki Oka , Shoichi Hazama , Ryouichi Tsunedomi
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6837 , C12N15/09 , C12Q1/68 , C12Q1/6816 , C12Q1/6832 , C12Q2527/107 , C12Q2527/125
摘要： A buffer composition for hybridization of a target nucleic acid is provided. The nucleic acid can include a nucleotide to be detected with a nucleic acid probe. The probe can contain a nucleotide sequence complementary to the target nucleic acid. The buffer can include a blocking nucleic acid having a nucleotide sequence complementary to a non-target nucleic acid having a nucleotide not to be detected corresponding to the nucleotide to be detected. The buffer composition can suppress non-specific hybridization to the nucleic acid probe even when a non-target nucleic acid is present. The use of the buffer composition can achieve excellent detection efficiency of the target nucleic acid.
摘要翻译： 提供了用于靶核酸杂交的缓冲组合物。 核酸可以包括用核酸探针检测的核苷酸。 探针可以含有与靶核酸互补的核苷酸序列。 缓冲液可以包括具有与非靶核酸互补的核苷酸序列的阻断核酸，其具有对应于待检测核苷酸的不被检测的核苷酸。 即使存在非靶核酸，缓冲组合物也可以抑制与核酸探针的非特异性杂交。 使用缓冲组合物可以实现目标核酸的优异检测效率。
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公开(公告)号：US11111526B2
公开(公告)日：2021-09-07
申请号：US16322363
申请日：2017-08-02
发明人： Toshiya Tsuda , Shuichi Kamei , Mitsuyoshi Oba , Hirofumi Yamano , Ryouichi Tsunedomi , Shoichi Hazama , Hiroaki Nagano
IPC分类号： C12Q1/68 , C12Q1/6832 , C07H21/00 , C12N15/09 , C12M1/00
摘要： In the case of using a blocking nucleic acid to prevent non-specific hybridization of a target nucleic acid with a nucleic acid probe, further excellent efficiency of detecting the target nucleic acid is achieved. A buffer composition used in hybridization of a target nucleic acid with a nucleic acid probe, wherein the buffer composition for hybridization contains a blocking nucleic acid comprising a nucleotide sequence complementary to a region comprising at least a non-detection target nucleotide in a non-target nucleic acid, in a concentration of one or more times higher than the concentration of a nucleic acid in a nucleic acid mixture consisting of the target nucleic acid and the non-target nucleic acid.
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公开(公告)号：US10450601B2
公开(公告)日：2019-10-22
申请号：US15024392
申请日：2014-09-01
IPC分类号： C12Q1/68 , C07H21/00 , C12Q1/6837 , C12N15/09 , C12Q1/6816 , C12Q1/6832
摘要： A buffer composition for hybridization of a target nucleic acid is provided. The nucleic acid can include a nucleotide to be detected with a nucleic acid probe. The probe can contain a nucleotide sequence complementary to the target nucleic acid. The buffer can include a blocking nucleic acid having a nucleotide sequence complementary to a non-target nucleic acid having a nucleotide not to be detected corresponding to the nucleotide to be detected. The buffer composition can suppress non-specific hybridization to the nucleic acid probe even when a non-target nucleic acid is present. The use of the buffer composition can achieve excellent detection efficiency of the target nucleic acid.
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