公开(公告)号：US07482156B2

公开(公告)日：2009-01-27

申请号：US10947812

申请日：2004-09-22

申请人： Trini Arroyo ,  Yuanhao Li

发明人： Trini Arroyo ,  Yuanhao Li

IPC分类号： C12N5/16 ,  C12N15/861

CPC分类号： C12N15/86 ,  A61K35/761 ,  A61K48/0058 ,  C07H21/04 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2840/203

摘要： Liver cancer, particularly hepatocellular carcinoma specific promoter sequences and adenovirus vehicles are provided. By providing for transcriptional initiating regulation dependent upon transcription factors that are only active in specific, limited cell types, virus replication will be restricted to the target cells. The modified adenovirus may be used as a vehicle for introducing new genetic capability, particularly associated with cytotoxicity, for treating neoplasia.

摘要翻译： 提供肝癌，特别是肝细胞癌特异性启动子序列和腺病毒载体。 通过提供依赖于仅在特定的有限细胞类型中有活性的转录因子的转录起始调节，病毒复制将限于靶细胞。 修饰的腺病毒可以用作引入新的遗传能力的载体，特别是与细胞毒性有关的遗传能力用于治疗瘤形成。

公开(公告)号：US20050124068A1

公开(公告)日：2005-06-09

申请号：US10947812

申请日：2004-09-22

申请人： Trini Arroyo ,  Yuanhao Li

发明人： Trini Arroyo ,  Yuanhao Li

IPC分类号： A61K35/76 ,  A61K35/761 ,  A61K48/00 ,  C07H21/04 ,  C12N5/08 ,  C12N7/00 ,  C12N15/861

CPC分类号： C12N15/86 ,  A61K35/761 ,  A61K48/0058 ,  C07H21/04 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2830/00 ,  C12N2830/008 ,  C12N2830/48 ,  C12N2830/85 ,  C12N2840/203

摘要： Liver cancer, particularly hepatocellular carcinoma specific promoter sequences and adenovirus vehicles are provided. By providing for transcriptional initiating regulation dependent upon transcription factors that are only active in specific, limited cell types, virus replication will be restricted to the target cells. The modified adenovirus may be used as a vehicle for introducing new genetic capability, particularly associated with cytotoxicity, for treating neoplasia.

摘要翻译： 提供肝癌，特别是肝细胞癌特异性启动子序列和腺病毒载体。 通过提供依赖于仅在特定的有限细胞类型中有活性的转录因子的转录起始调节，病毒复制将限于靶细胞。 修饰的腺病毒可以用作引入新的遗传能力的载体，特别是与细胞毒性有关的遗传能力用于治疗瘤形成。

公开(公告)号：US20090270485A1

公开(公告)日：2009-10-29

申请号：US12220686

申请日：2008-07-24

申请人： Derek Ko ,  Yuanhao Li ,  Thomas Harding ,  Jianmin Fang ,  Nagarajan Ramesh ,  De-Chao Yu

发明人： Derek Ko ,  Yuanhao Li ,  Thomas Harding ,  Jianmin Fang ,  Nagarajan Ramesh ,  De-Chao Yu

IPC分类号： A61K31/7088 ,  C12N15/63 ,  C12N5/10

CPC分类号： C12N15/86 ,  A61K35/761 ,  A61K38/162 ,  A61K38/193 ,  C12N7/00 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2770/32134 ,  C12N2830/008 ,  C12N2830/20 ,  A61K2300/00

摘要： Cell specific replication-competent viral vectors comprising a self processing peptide cleavage sequence are provided. The targeted replication-competent viral vectors include two or more co-transcribed genes under transcriptional control of the same heterologous transcriptional regulatory element (TRE), wherein at least a second gene is under translational control of a self processing cleavage sequence or 2A sequence. Exemplary vector constructs may further include an additional proteolytic cleavage site which provides a means to remove the self processing peptide sequence from the viral vector.

摘要翻译： 提供了包含自身加工肽切割序列的细胞特异性复制能力的病毒载体。 靶向复制能力的病毒载体包括在相同异源转录调控元件（TRE）的转录控制下的两个或多个共转录基因，其中至少第二个基因处于自身加工切割序列或2A序列的翻译控制下。 示例性的载体构建体可进一步包括额外的蛋白水解切割位点，其提供从病毒载体中除去自身加工肽序列的手段。

公开(公告)号：US20090099125A1

公开(公告)日：2009-04-16

申请号：US12327936

申请日：2008-12-04

申请人： DeChao Yu ,  Yuanhao Li

发明人： DeChao Yu ,  Yuanhao Li

IPC分类号： A61K31/7088 ,  C12N15/63 ,  C12N5/02

CPC分类号： C12N15/86 ,  A61K48/005 ,  C12N7/00 ,  C12N2710/10321 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2830/00 ,  C12N2830/001 ,  C12N2830/85 ,  C12N2840/203

摘要： Replication-competent adenoviral vectors which selectively replicate in cancer cells are provided. The replication-competent viral vectors comprise an E2F responsive promoter and/or a telomerase promoter operatively linked to an adenoviral coding region. The replication-competent adenoviral vectors effectively replicate in a variety of types of cancer cells and find broad utility in the treatment of cancer.

摘要翻译： 提供了在癌细胞中选择性复制的复制能力的腺病毒载体。 复制能力的病毒载体包含可操作地连接到腺病毒编码区的E2F应答启动子和/或端粒酶启动子。 复制能力的腺病毒载体在多种类型的癌细胞中有效地复制，并且在治疗癌症中发现广泛的用途。

公开(公告)号：US07074618B2

公开(公告)日：2006-07-11

申请号：US10857137

申请日：2004-05-28

申请人： Yuanhao Li ,  Deborah Farson ,  Luqun Tao ,  De-Chao Yu

发明人： Yuanhao Li ,  Deborah Farson ,  Luqun Tao ,  De-Chao Yu

IPC分类号： C12N15/861 ,  C12N15/867 ,  C12N5/10 ,  C12N15/63 ,  C12N15/64

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/48 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/44

摘要： Adenovirus packaging cell lines for growth of E1A/E1B deficient adenovirus that is substantially free of replication competent adenovirus (RCA), are provided. Methods for producing adenovirus substantially free of RCA are also provided, wherein the adenovirus is grown in a cell line containing coding sequences for adenovirus E1A and E1B, are operably linked to promoters that lack polynucleotide sequences sharing substantial sequence identity with the native adenovirus E1A and E1B promoters.

摘要翻译： 提供了基本上不含复制性腺病毒（RCA）的E1A / E1B缺陷型腺病毒生长的腺病毒包装细胞系。 还提供了用于产生基本上不含RCA的腺病毒的方法，其中腺病毒在含有腺病毒E1A和E1B编码序列的细胞系中生长，可操作地连接于缺乏与天然腺病毒E1A和E1B具有大体序列同一性的多核苷酸序列的启动子 发起人。

公开(公告)号：US20090156796A1

公开(公告)日：2009-06-18

申请号：US12011966

申请日：2008-01-29

申请人： Yuanhao Li ,  De-Chao Yu

发明人： Yuanhao Li ,  De-Chao Yu

IPC分类号： C07H21/04

CPC分类号： C12N15/86 ,  A61K48/00 ,  A61K48/0058 ,  C12N2710/10343 ,  C12N2710/10345 ,  C12N2830/008 ,  C12N2830/85

摘要： Colon cancer specific promoter sequences and adenovirus vehicles are provided. By providing for transcriptional initiating regulation dependent upon transcription factors that are only active in specific, limited cell types, virus replication will be restricted to the target cells. The modified adenovirus may be used as a vehicle for introducing new genetic capability, particularly associated with cytotoxicity for treating neoplasia.

摘要翻译： 提供结肠癌特异性启动子序列和腺病毒载体。 通过提供依赖于仅在特定的有限细胞类型中有活性的转录因子的转录起始调节，病毒复制将限于靶细胞。 修饰的腺病毒可以用作引入新遗传能力的载体，特别是与用于治疗瘤形成的细胞毒性有关。

公开(公告)号：US07473418B2

公开(公告)日：2009-01-06

申请号：US11074694

申请日：2005-03-09

申请人： DeChao Yu ,  Yuanhao Li

发明人： DeChao Yu ,  Yuanhao Li

IPC分类号： A01N63/00 ,  A01N43/04 ,  A61K48/00 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/86 ,  A61K48/005 ,  C12N7/00 ,  C12N2710/10321 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2830/00 ,  C12N2830/001 ,  C12N2830/85 ,  C12N2840/203

摘要： Replication-competent adenoviral vectors which selectively replicate in cancer cells are provided. The replication-competent viral vectors comprise an E2F responsive promoter and/or a telomerase promoter operatively linked to an adenoviral coding region. The replication-competent adenoviral vectors effectively replicate in a variety of types of cancer cells and find broad utility in the treatment of cancer.

摘要翻译： 提供了在癌细胞中选择性复制的复制能力的腺病毒载体。 复制能力的病毒载体包含可操作地连接到腺病毒编码区的E2F应答启动子和/或端粒酶启动子。 复制能力的腺病毒载体在多种类型的癌细胞中有效地复制，并且在治疗癌症中发现广泛的用途。

公开(公告)号：US20060024829A1

公开(公告)日：2006-02-02

申请号：US11240406

申请日：2005-10-03

申请人： Yuanhao Li ,  Deborah Farson ,  Luqun Tao ,  DeChao Yu

发明人： Yuanhao Li ,  Deborah Farson ,  Luqun Tao ,  DeChao Yu

IPC分类号： C12N15/861 ,  C12N5/06

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/48 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/44

摘要： Adenovirus packaging cell lines for growth of an E1A/E1B deficient adenovirus that is substantially free of replication competent adenovirus (RCA) are provided. Methods for producing adenovirus substantially free of RCA are also provided, wherein the adenovirus is grown in a cell line containing coding sequences for adenovirus E1A and E1B, which are operably linked to promoters that lack polynucleotide sequences sharing substantial sequence identity with the native adenovirus E1A and E1B promoters.

公开(公告)号：US07132284B2

公开(公告)日：2006-11-07

申请号：US11240406

申请日：2005-10-03

申请人： Yuanhao Li ,  Deborah Farson ,  Luqun Tao ,  DeChao Yu

发明人： Yuanhao Li ,  Deborah Farson ,  Luqun Tao ,  DeChao Yu

IPC分类号： C12N15/63 ,  C12N15/64 ,  C12N15/861 ,  C12N7/01 ,  C12N5/10 ,  A61K48/00

CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10343 ,  C12N2710/10352 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/48 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/44

摘要： Adenovirus packaging cell lines for growth of an E1A/E1B deficient adenovirus that is substantially free of replication competent adenovirus (RCA) are provided. Methods for producing adenovirus substantially free of RCA are also provided, wherein the adenovirus is grown in a cell line containing coding sequences for adenovirus E1A and E1B, which are operably linked to promoters that lack polynucleotide sequences sharing substantial sequence identity with the native adenovirus E1A and E1B promoters.

摘要翻译： 提供了基本上不含复制型腺病毒（RCA）的E1A / E1B缺陷型腺病毒生长的腺病毒包装细胞系。 还提供了用于产生基本上不含RCA的腺病毒的方法，其中腺病毒在含有腺病毒E1A和E1B编码序列的细胞系中生长，所述编码序列与腺病毒E1A和E1B的编码序列可操作地连接，所述编码序列与缺乏与天然腺病毒E1A具有实质序列同一性的多核苷酸序列的启动子连接， E1B启动子。

公开(公告)号：US20050136035A1

公开(公告)日：2005-06-23

申请号：US10857498

申请日：2004-06-01

申请人： Derek Ko ,  Yuanhao Li ,  Thomas Harding ,  Jianmin Fang ,  Nagarajan Ramesh ,  De Yu

发明人： Derek Ko ,  Yuanhao Li ,  Thomas Harding ,  Jianmin Fang ,  Nagarajan Ramesh ,  De Yu

IPC分类号： A01N63/00 ,  A61K35/76 ,  A61K35/761 ,  A61K38/16 ,  A61K48/00 ,  C12N20060101 ,  C12N5/00 ,  C12N5/08 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/64 ,  C12N15/66 ,  C12N15/70 ,  C12N15/74 ,  C12N15/861

CPC分类号： C12N15/86 ,  A61K35/761 ,  A61K38/162 ,  A61K38/193 ,  C12N7/00 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2770/32134 ,  C12N2830/008 ,  C12N2830/20 ,  A61K2300/00

摘要： Cell specific replication-competent viral vectors comprising a self processing peptide cleavage sequence are provided. The targeted replication-competent viral vectors include two or more co-transcribed genes under transcriptional control of the same heterologous transcriptional regulatory element (TRE), wherein at least a second gene is under translational control of a self processing cleavage sequence or 2A sequence. Exemplary vector constructs may further include an additional proteolytic cleavage site which provides a means to remove the self processing peptide sequence from the viral vector.

摘要翻译： 提供了包含自身加工肽切割序列的细胞特异性复制能力的病毒载体。 靶向复制能力的病毒载体包括在相同异源转录调控元件（TRE）的转录控制下的两个或多个共转录基因，其中至少第二个基因处于自身加工切割序列或2A序列的翻译控制下。 示例性的载体构建体可进一步包括额外的蛋白水解切割位点，其提供从病毒载体中除去自身加工肽序列的手段。