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    Methods and/or Systems Producing and Providing Sets of Oligonucleotide Conjugates for Assays and Detections
    5.
    发明申请
    Methods and/or Systems Producing and Providing Sets of Oligonucleotide Conjugates for Assays and Detections 审中-公开
    生产和提供测定和检测的寡核苷酸缀合物的方法和/或系统

    公开(公告)号：US20130035259A1

    公开(公告)日：2013-02-07

    申请号：US13302851

    申请日：2011-11-22

    申请人： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

    发明人： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

    IPC分类号： C40B40/06 ,  C40B50/00

    CPC分类号： C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

    摘要： The present disclosure is directed to methods and/or systems producing and providing uses sets of oligonucleotide conjugates for assays and detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

    摘要翻译： 本公开涉及产生和提供用于测定和检测以及相关系统和/或试剂盒的寡核苷酸缀合物组的方法和/或系统。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

    Methods and/or Use of Oligonucleotide Conjugates for Assays and Flow Cytometry Detections
    6.
    发明申请
    Methods and/or Use of Oligonucleotide Conjugates for Assays and Flow Cytometry Detections 审中-公开
    用于测定和流式细胞术检测的寡核苷酸缀合物的方法和/或用途

    公开(公告)号：US20120258880A1

    公开(公告)日：2012-10-11

    申请号：US13302877

    申请日：2011-11-22

    申请人： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

    发明人： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

    IPC分类号： G01N33/53 ,  C40B30/04 ,  G01N21/47 ,  G01N21/59 ,  G01N21/64 ,  G01N27/26 ,  G01N24/00 ,  G01N21/65 ,  G01N21/66 ,  G01N21/25 ,  G01N27/72 ,  C12Q1/68 ,  G01N21/76 ,  B82Y15/00

    CPC分类号： C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

    摘要： The present disclosure is directed to methods and/or uses of oligonucleotide conjugates for assays and flow cytometry detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

    摘要翻译： 本公开涉及寡核苷酸缀合物用于测定和流式细胞术检测及相关系统和/或试剂盒的方法和/或用途。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

    Method for optically manipulating polymer filaments
    7.
    发明授权
    Method for optically manipulating polymer filaments 失效
    光学操作聚合物长丝的方法

    公开(公告)号：US5079169A

    公开(公告)日：1992-01-07

    申请号：US528316

    申请日：1990-05-22

    申请人： Steven Chu ,  Stephen J. Kron

    发明人： Steven Chu ,  Stephen J. Kron

    IPC分类号： H05H3/04

    CPC分类号： H05H3/04 ,  Y10T436/25

    摘要： Method and apparatus for manipulating a microscopic particle by single-beam gradient optical trapping, using an optical beam whose trapping force is substantially independent of position within a view field. The apparatus may be used to extend a polymer filament, and to fix the extended filament at a selected stretching force. When applied to nucleic acid filament, the method may be employed for genomic DNA mapping of filaments up to several megabasepairs in size. The method may also be used for studying the interaction of enzymes or ribosomes with extended DNA in real time.

    摘要翻译： 用于通过单光束梯度光学捕获来操纵微观粒子的方法和装置，其使用捕获力基本上与视场内的位置无关的光束。 该装置可用于延伸聚合物长丝，并将延伸的细丝固定在选定的拉伸力。 当应用于核酸细丝时，该方法可用于大小达几兆巴的细丝的基因组DNA定位。 该方法还可用于实时研究酶或核糖体与延伸DNA的相互作用。

    Methods and/or Use of Oligonucleotide Conjugates for Assays and Microscopy/Imaging Detections
    8.
    发明申请
    Methods and/or Use of Oligonucleotide Conjugates for Assays and Microscopy/Imaging Detections 审中-公开
    用于测定和显微镜/成像检测的寡核苷酸缀合物的方法和/或用途

    公开(公告)号：US20120258881A1

    公开(公告)日：2012-10-11

    申请号：US13302890

    申请日：2011-11-22

    申请人： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

    发明人： David A. Schwartz ,  Jimmy Williams ,  Xinfang Zhao ,  Chunfang Zhao ,  William B. Busa ,  Stephen J. Kron ,  Amy Catherine Flor

    IPC分类号： C12Q1/68 ,  G01N21/76 ,  G01N21/00 ,  G01N21/59 ,  G01N21/47 ,  G01N21/64 ,  G01N21/25 ,  G01N21/66 ,  G01N21/65 ,  G01N24/00 ,  G01N27/72 ,  C40B30/04 ,  C12Q1/70 ,  B82Y15/00

    CPC分类号： C12Q1/6804 ,  C07H21/00 ,  C12Q1/6834 ,  C12Q2565/113

    摘要： The present disclosure is directed to methods and/or uses of oligonucleotide conjugates for assays and microscopy/imaging detections and related systems and/or kits. Certain methods are directed to a method for detecting one or more biological targets of a sample in a detection assay, comprising: providing a molecular probe, comprising a binding moiety and an oligonucleotide sequence, to a sample comprising one or more biological targets; binding the one or more biological targets with the binding moiety; providing a detectable component to the sample, wherein the detectable component comprises a signal generating moiety conjugated to an oligonucleotide sequence complementary to the oligonucleotide sequence of the molecular probe; hydridizing the oligonucleotide sequence of the target-bound molecular probe to the detectable component; and detecting a signal generated from the hydridized detectable component. Various other embodiments, applications etc. are disclosed herein.

    摘要翻译： 本公开涉及用于测定和显微镜/成像检测和相关系统和/或试剂盒的寡核苷酸缀合物的方法和/或用途。 某些方法涉及用于在检测测定中检测样品的一种或多种生物学靶标的方法，其包括：向包含一种或多种生物靶标的样品提供包含结合部分和寡核苷酸序列的分子探针; 将一种或多种生物靶与结合部分结合; 向所述样品提供可检测组分，其中所述可检测组分包含与与所述分子探针的寡核苷酸序列互补的寡核苷酸序列缀合的信号产生部分; 将靶结合分子探针的寡核苷酸序列杂交到可检测组分; 以及检测由所述可混合的可检测组分产生的信号。 本文公开了各种其它实施例，应用等。

    Hydrogels for biomolecule analysis and corresponding method to analyze biomolecules
    9.
    发明授权
    Hydrogels for biomolecule analysis and corresponding method to analyze biomolecules 有权
    用于生物分子分析的水凝胶和分析生物分子的相应方法

    公开(公告)号：US07588906B2

    公开(公告)日：2009-09-15

    申请号：US11305671

    申请日：2005-12-16

    申请人： Shawn B. Brueggemeier ,  Stephen J. Kron ,  Sean P. Palecek ,  Laurie Parker ,  Stephen Brian Henry Kent

    发明人： Shawn B. Brueggemeier ,  Stephen J. Kron ,  Sean P. Palecek ,  Laurie Parker ,  Stephen Brian Henry Kent

    IPC分类号： C12Q1/37 ,  G01N33/53

    CPC分类号： G01N33/6842 ,  G01N33/6851 ,  H01J49/04

    摘要： Polyacrylamide-based methods of fabricating surface-bound peptide and protein arrays, the arrays themselves, and a method of using the arrays to detect biomolecules and to measure their concentration, binding affinity, and kinetics are described. Peptides, proteins, fusion proteins, protein complexes, nucleic acids, and the like, are labeled with an acrylic moiety and attached to acrylic-functionalized glass surfaces through a copolymerization with acrylic monomer. The specific attachment of glutathione S-transferase-green fluorescent protein (GST-GFP) fusion protein was more than 7-fold greater than the nonspecific attachment of non-acrylic labeled GST-GFP. Surface-attached GST-GFP (0.32 ng/mm2) was detectable by direct measurement of green fluorescent protein fluorescence and this lower detection limit was reduced to 0.080 ng/mm2 using indirect antibody-based detection. The polyacrylamide-based surface attachment strategy was also used to measure the kinetics of substrate phosphorylation by the kinase c-Src which is encoded by the Rous Sarcoma virus. The surface attachment strategy is applicable to the proteomics field and addresses denaturation and dehydration problems associated with protein microarray development.

    摘要翻译： 描述了基于聚丙烯酰胺的制备表面结合肽和蛋白质阵列的方法，阵列本身以及使用阵列检测生物分子并测量其浓度，结合亲和力和动力学的方法。 肽，蛋白质，融合蛋白，蛋白质复合物，核酸等用丙烯酸部分标记，并通过与丙烯酸类单体的共聚连接到丙烯酸官能化的玻璃表面上。 谷胱甘肽S-转移酶绿色荧光蛋白（GST-GFP）融合蛋白的特异性附着比非丙烯酸标记的GST-GFP的非特异性附着大7倍以上。 通过直接测量绿色荧光蛋白荧光检测表面附着的GST-GFP（0.32ng / mm2），并使用间接抗体检测将该检测下限降低至0.080ng / mm2。 基于聚丙烯酰胺的表面附着策略也用于通过由Rous肉瘤病毒编码的激酶c-Src来测量底物磷酸化的动力学。 表面附着策略适用于蛋白质组学领域，涉及与蛋白质微阵列开发相关的变性和脱水问题。

    Nucleic acid-containing polymerizable complex
    10.
    发明授权
    Nucleic acid-containing polymerizable complex 有权
    含有核酸的可聚合复合物

    公开(公告)号：US06180770B2

    公开(公告)日：2001-01-30

    申请号：US09183092

    申请日：1998-10-30

    申请人： T. Christian Boles ,  Stephen J. Kron ,  Christopher P. Adams

    发明人： T. Christian Boles ,  Stephen J. Kron ,  Christopher P. Adams

    IPC分类号： C12Q168

    CPC分类号： C07H21/00 ,  C07H21/02

    摘要： Disclosed is a polymerizable complex containing a covalently attached nucleic acid molecule which, under appropriate conditions, is capable of copolymerization with a second polymerizable ethylene-containing monomer unit to form a polymerized layer. The polymerized layer containing attached nucleic acid is useful in a variety of contexts including, for example, hybridization assays. The polymerized layer containing the covalently attached nucleic acid molecule can be formed into a variety of shapes, or attached to a formed material through appropriate chemistry.

    摘要翻译： 公开了含有共价连接的核酸分子的可聚合复合物，其在合适的条件下能够与第二可聚合的含乙烯单体单元共聚以形成聚合层。 含有连接核酸的聚合层可用于多种情况，包括例如杂交测定。 含有共价连接的核酸分子的聚合层可以形成多种形状，或通过适当的化学成分连接到成形材料上。