PROTEASE, DNA ENCODING THE SAME, AND METHOD FOR MANUFACTURING PROTEASE
    3.
    发明申请
    PROTEASE, DNA ENCODING THE SAME, AND METHOD FOR MANUFACTURING PROTEASE 有权
    PROTEASE,编码其的DNA,以及制造蛋白质的方法

    公开(公告)号:US20100015664A1

    公开(公告)日:2010-01-21

    申请号:US12537259

    申请日:2009-08-07

    CPC分类号: C12N9/52

    摘要: A protease having the following characteristics: (i) a molecular weight of 21,000±1,000 (by SDS-PAGE) and (ii) specific protease activity cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (1) below, cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (2) below, or cutting the Ser-Leu site of a peptide or protein comprising amino acid sequence (3) below: Pro-Gln-Gly-Phe-Gln-Gly-Pro(1) (SEQ ID NO: 4) Pro-Ala-Gly-Phe-Ala-Gly-Pro(2) (SEQ ID NO: 5) Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro.(3) (SEQ ID NO: 6) DNA encoding this protease, including vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.

    摘要翻译: 具有以下特征的蛋白酶:(i)分子量为21,000±1,000(通过SDS-PAGE)和(ii)切割包含以下氨基酸序列(1)的肽或蛋白质的Gly-Phe位点的特异性蛋白酶活性, 切割包含以下氨基酸序列(2)的肽或蛋白质的Gly-Phe位点,或切割包含以下氨基酸序列(3)的肽或蛋白质的Ser-Leu位点:Pro-Gln-Gly-Phe-Gln -Gly-Pro(1)(SEQ ID NO:4)Pro-Ala-Gly-Phe-Ala-Gly-Pro(2)(SEQ ID NO:5)Gln-Thr-Gln-Ser-Leu-Val-Thr -Pro。 (3)(SEQ ID NO:6)编码该蛋白酶的DNA,包含含有该DNA的载体,以及通过用该DNA转化细胞产生该蛋白酶的方法。

    DNA encoding a bacterial protease for degrading gelatin and collagen
    4.
    发明授权
    DNA encoding a bacterial protease for degrading gelatin and collagen 有权
    编码细菌蛋白酶降解凝胶和胶原的DNA

    公开(公告)号:US07871797B2

    公开(公告)日:2011-01-18

    申请号:US12537259

    申请日:2009-08-07

    IPC分类号: C12P21/02

    CPC分类号: C12N9/52

    摘要: The present invention relates to a protease having a molecular weight of 21,000±1,000 (by SDS-PAGE) and a specific protease activity capable of cutting at the Gly-Phe site of a peptide or protein comprising the amino acid sequence Pro-Gln-Gly-Phe-Gln-Gly-Pro or Pro-Ala-Gly-Phe-Ala-Gly-Pro or cutting at the Ser-Leu site of a peptide or protein comprising the amino acid sequence Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro. The invention further provides DNA encoding this protease, as well as vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.

    摘要翻译: 本发明涉及分子量为21,000±1,000(通过SDS-PAGE)的蛋白酶和能够在包含氨基酸序列Pro-Gln-Gly的肽或蛋白质的Gly-Phe位点切割的特异性蛋白酶活性 -Phe-Gln-Gly-Pro或Pro-Ala-Gly-Phe-Ala-Gly-Pro或在包含氨基酸序列Gln-Thr-Gln-Ser-Leu- Val-Thr-Pro。 本发明还提供了编码该蛋白酶的DNA,以及含有该DNA的载体,以及通过用该DNA转化细胞来生产该蛋白酶的方法。

    Protease, DNA encoding the same, and method for manufacturing protease
    6.
    发明授权
    Protease, DNA encoding the same, and method for manufacturing protease 有权
    蛋白酶,编码相同的DNA,以及制造蛋白酶的方法

    公开(公告)号:US07601807B2

    公开(公告)日:2009-10-13

    申请号:US11287307

    申请日:2005-11-28

    IPC分类号: C07K1/00

    CPC分类号: C12N9/52

    摘要: A protease having the following characteristics: (i) a molecular weight of 21,000±1,000 (by SDS-PAGE) and (ii) specific protease activity cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (1) below, cutting the Gly-Phe site of a peptide or protein comprising amino acid sequence (2) below, or cutting the Ser-Leu site of a peptide or protein comprising amino acid sequence (3) below: (1) (SEQ ID NO:4) Pro-Gln-Gly-Phe-Gln-Gly-Pro (2) (SEQ ID NO:5) Pro-Ala-Gly-Phe-Ala-Gly-Pro (3) (SEQ ID NO:6) Gln-Thr-Gln-Ser-Leu-Val-Thr-Pro. DNA encoding this protease, including vectors containing this DNA, and a method for producing this protease by transforming a cell with this DNA.

    摘要翻译: 具有以下特征的蛋白酶:(i)分子量为21,000±1,000(通过SDS-PAGE)和(ii)切割包含以下氨基酸序列(1)的肽或蛋白质的Gly-Phe位点的特异性蛋白酶活性, 切割包含以下氨基酸序列(2)的肽或蛋白质的Gly-Phe位点,或切割包含以下氨基酸序列(3)的肽或蛋白质的Ser-Leu位点:(1)(SEQ ID NO:4 )Pro-Gln-Gly-Phe-Gln-Gly-Pro(2)(SEQ ID NO:5)Pro-Ala-Gly-Phe-Ala-Gly-Pro(3)(SEQ ID NO:6)Gln-Thr -Gln-Ser-Leu-Val-Thr-Pro。 编码该蛋白酶的DNA,包括含有该DNA的载体,以及通过用该DNA转化细胞来生产该蛋白酶的方法。