公开(公告)号：US10073082B2

公开(公告)日：2018-09-11

申请号：US15028846

申请日：2014-10-03

Applicant: UNIVERSITY HEALTH NETWORK ,  BECKMAN COULTER, INC.

Inventor： T. Vincent Shankey ,  Sergei Gulnik ,  Lidice L. Lopez ,  David W. Hedley ,  Sue Chow

IPC: G01N33/49 ,  G01N33/53 ,  G01N33/50 ,  C12Q1/37 ,  G01N33/58 ,  G01N15/14 ,  C07K14/47 ,  C07K16/18 ,  G01N15/00 ,  G01N15/10

CPC classification number: G01N33/5041 ,  C07K14/47 ,  C07K16/18 ,  C12Q1/37 ,  G01N15/14 ,  G01N33/4915 ,  G01N33/5011 ,  G01N33/5047 ,  G01N33/53 ,  G01N33/582 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2333/4704 ,  G01N2440/14

Abstract: Assays that can measure the effect of proteasome inhibitors on target cells in a biological sample are provided. The assays include evaluation of the effects of proteasome inhibitors on proteasome activity in cells in a biological sample.

公开(公告)号：US09040249B2

公开(公告)日：2015-05-26

申请号：US13665776

申请日：2012-10-31

Applicant: Beckman Coulter, Inc. ,  University Health Network

Inventor： David Hedley ,  Sue Chow ,  T. Vincent Shankey

IPC: G01N33/50 ,  G01N33/573 ,  G01N33/569

CPC classification number: G01N33/5008 ,  G01N33/5041 ,  G01N33/5047 ,  G01N33/56972 ,  G01N33/573

Abstract: Methods and reagents are provided for determining the activation state of a signal transduction pathway signaling protein. There exists a need in the art for methods that can monitor the efficacy of a signal transduction inhibitor in a patient. Other needs exist for detecting and monitoring certain disease or disorders that are associated with aberrant activation of a signal transduction pathway signaling protein. The present assay provides a highly sensitive assay that is also useful in patient populations in which obtaining a large cellular sample is difficult, for example, neonates.

Abstract translation: 提供了用于确定信号转导途径信号传导蛋白的激活状态的方法和试剂。 在本领域中存在对可以监测信号转导抑制剂在患者中的功效的方法的需要。 存在用于检测和监测与信号转导途径信号蛋白的异常激活相关的某些疾病或病症的其它需要。 本测定提供了高度敏感的测定法，其也可用于难以获得大细胞样品的患者群体，例如新生儿。

公开(公告)号：US20130295582A1

公开(公告)日：2013-11-07

申请号：US13665776

申请日：2012-10-31

Applicant: University Health Network ,  Beckman Coulter, Inc.

Inventor： David Hedley ,  Sue Chow ,  T. Vincent Shankey

IPC: G01N33/50

CPC classification number: G01N33/5008 ,  G01N33/5041 ,  G01N33/5047 ,  G01N33/56972 ,  G01N33/573

Abstract: Methods and reagents are provided for determining the activation state of a signal transduction pathway signaling protein. There exists a need in the art for methods that can monitor the efficacy of a signal transduction inhibitor in a patient. Other needs exist for detecting and monitoring certain disease or disorders that are associated with aberrant activation of a signal transduction pathway signaling protein. The present assay provides a highly sensitive assay that is also useful in patient populations in which obtaining a large cellular sample is difficult, for example, neonates.

Abstract translation: 提供了用于确定信号转导途径信号传导蛋白的激活状态的方法和试剂。 在本领域中存在对可以监测信号转导抑制剂在患者中的功效的方法的需要。 存在用于检测和监测与信号转导途径信号蛋白的异常激活相关的某些疾病或病症的其它需要。 本测定提供了高度敏感的测定法，其也可用于难以获得大细胞样品的患者群体，例如新生儿。