公开(公告)号：US10794827B2

公开(公告)日：2020-10-06

申请号：US15478953

申请日：2017-04-04

申请人： University of Washington

发明人： Thomas Matula ,  Andrew A. Brayman ,  Oleg A. Sapozhnikov ,  Brian MacConaghy ,  Jarred Egan Swalwell ,  Camilo Perez

IPC分类号： G01N21/53 ,  G01N15/10 ,  G01N1/40 ,  G01N21/64 ,  B01L3/00

摘要： Embodiments are generally related to differentiating and/or separating portions of a sample that are of interest from the remainder of the sample. Embodiments may be directed towards separating cells of interest from a cell sample. In some embodiments, acoustic impedances of the cells of interest may be modified. For example, the acoustic properties of the cells of interest may be modified by attaching bubbles to the cells of interest. The cell sample may then be subjected to an acoustic wave. The cells of interest may be differentiated and/or separated from the remainder of the sample based on relative displacements and/or volumetric changes experienced by the cells of interest in response thereto. The cells of interest may be separated using a standing wave and sorted into separate channels of a flow cell. Optionally, the cells may be interrogated by a light source and differentiated by signals generated in response thereto.

公开(公告)号：US20170328834A1

公开(公告)日：2017-11-16

申请号：US15478953

申请日：2017-04-04

申请人： University of Washington

发明人： Thomas Matula ,  Andrew A. Brayman ,  Oleg A. Sapozhnikov ,  Brian MacConaghy ,  Jarred Egan Swalwell ,  Camilo Perez

IPC分类号： G01N21/53 ,  B01L3/00 ,  G01N21/64 ,  G01N1/40 ,  G01N15/10

摘要： Embodiments are generally related to differentiating and/or separating portions of a sample that are of interest from the remainder of the sample. Embodiments may be directed towards separating cells of interest from a cell sample. In some embodiments, acoustic impedances of the cells of interest may be modified. For example, the acoustic properties of the cells of interest may be modified by attaching bubbles to the cells of interest. The cell sample may then be subjected to an acoustic wave. The cells of interest may be differentiated and/or separated from the remainder of the sample based on relative displacements and/or volumetric changes experienced by the cells of interest in response thereto. The cells of interest may be separated using a standing wave and sorted into separate channels of a flow cell. Optionally, the cells may be interrogated by a light source and differentiated by signals generated in response thereto.

公开(公告)号：US09645080B2

公开(公告)日：2017-05-09

申请号：US14254611

申请日：2014-04-16

申请人： University of Washington Through Its Center for Commercialization

发明人： Thomas Matula ,  Andrew A. Brayman ,  Oleg A. Sapozhnikov ,  Brian MacConaghy ,  Jarred Egan Swalwell ,  Camilo Perez

IPC分类号： G01N33/00 ,  G01N21/53 ,  G01N21/64 ,  G01N1/40 ,  B01L3/00 ,  G01N15/10

CPC分类号： G01N21/53 ,  B01L3/502723 ,  B01L3/502761 ,  B01L3/502784 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2300/0816 ,  B01L2300/0883 ,  B01L2400/0436 ,  G01N1/4077 ,  G01N21/6486 ,  G01N2001/4066 ,  G01N2015/1081 ,  G01N2021/6439

摘要： Embodiments are generally related to differentiating and/or separating portions of a sample that are of interest from the remainder of the sample. Embodiments may be directed towards separating cells of interest from a cell sample. In some embodiments, acoustic impedances of the cells of interest may be modified. For example, the acoustic properties of the cells of interest may be modified by attaching bubbles to the cells of interest. The cell sample may then be subjected to an acoustic wave. The cells of interest may be differentiated and/or separated from the remainder of the sample based on relative displacements and/or volumetric changes experienced by the cells of interest in response thereto. The cells of interest may be separated using a standing wave and sorted into separate channels of a flow cell. Optionally, the cells may be interrogated by a light source and differentiated by signals generated in response thereto.