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    METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING
    5.
    发明申请
    METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING 有权

    公开(公告)号：US20220290231A1

    公开(公告)日：2022-09-15

    申请号：US17451919

    申请日：2021-10-22

    申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

    发明人： Jesse SALK ,  Lawrence A. LOEB ,  Michael SCHMITT

    IPC分类号： C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

    摘要： Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.

    Extended depth of focus for high-resolution optical image scanning
    7.
    发明授权
    Extended depth of focus for high-resolution optical image scanning 有权

    公开(公告)号：US11330170B2

    公开(公告)日：2022-05-10

    申请号：US16708728

    申请日：2019-12-10

    申请人： University of Washington through its Center for Commercialization

    发明人： Eric J. Seibel ,  Brian T. Schowengerdt

    IPC分类号： H04N5/232 ,  G02B23/24 ,  G02B23/26 ,  G02B27/40 ,  A61B1/06 ,  A61B1/07 ,  G02B26/10 ,  A61B1/00 ,  G03B21/14

    摘要： Methods and systems for acquiring and/or projecting images from and/or to a target area are provided. Such a method or system can include an optical fiber assembly which may be driven to scan the target area in a scan pattern. The optical fiber assembly may provide multiple effective light sources (e.g., via a plurality of optical fibers) that are axially staggered with respect to an optical system located between the optical fiber and the target area. The optical system may be operable to focus and/or redirect the light from the multiple light sources onto separate focal planes. A composite image may be generated based on light reflected from and/or projected onto the separate focal planes. The composite image may have an extended depth of focus or field spanning over a distance between the separate focal planes while maintaining or improving image resolution.

    METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING
    9.
    发明申请
    METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING 有权

    公开(公告)号：US20220010376A1

    公开(公告)日：2022-01-13

    申请号：US17448973

    申请日：2021-09-27

    申请人： UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

    发明人： Jesse SALK ,  Lawrence A. LOEB ,  Michael SCHMITT

    IPC分类号： C12Q1/6876 ,  C12Q1/6806 ,  C12Q1/6869

    摘要： Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands.