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公开(公告)号：US11906523B2
公开(公告)日：2024-02-20
申请号：US16856589
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
CPC分类号： G01N33/581 , C12Q1/68 , C12Q1/682 , G01N33/53 , G01N33/542 , C07H21/02 , C07K14/00
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US10982269B2
公开(公告)日：2021-04-20
申请号：US15245126
申请日：2016-08-23
发明人： Michael Farrell , William Day , Zeyu Jiang , Anne Pedata
IPC分类号： C12Q1/68 , C07H21/00 , C12Q1/6841 , C12Q1/6886 , C12Q1/6816 , C12Q1/6804 , C12Q1/682
摘要： Disclosed herein are methods and compositions for detecting differential expression of certain miRNAs in cancer cells or their surrounding normal tissues in the tumor microenvironment. The disclosure describes an automated, highly sensitive and specific method for detection of any cellular RNA molecule, including microRNA, messenger RNA and non-coding RNA. The technology includes probe design as well as probe use in an automated fashion for detection of RNA molecules in formalin-fixed paraffin-embedded tissue (FFPET) samples.
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公开(公告)号：US20200326345A1
公开(公告)日：2020-10-15
申请号：US16786647
申请日：2020-02-10
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20190018018A1
公开(公告)日：2019-01-17
申请号：US16038374
申请日：2018-07-18
发明人： Nelson ALEXANDER , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , G01N33/542 , C12Q1/68 , G01N33/53 , C12Q1/682
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20220315987A1
公开(公告)日：2022-10-06
申请号：US17805141
申请日：2022-06-02
发明人： William Day , Megan C. Peccarelli
IPC分类号： C12Q1/6806 , C12N15/10
摘要： Automated methods for extracting nucleic acid from one or more tissue samples disposed on slides are disclosed. The methods utilize an automated slide staining apparatus that dispenses a plurality of nucleic acid extraction reagents onto the tissue sample, thus creating an extracted nucleic acid sample. The extracted nucleic acid sample may be used directly in downstream applications such as nucleic acid amplification or sequencing procedures, or may be further purified.
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公开(公告)号：US20200249238A1
公开(公告)日：2020-08-06
申请号：US16856589
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20130260379A1
公开(公告)日：2013-10-03
申请号：US13849160
申请日：2013-03-22
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58
CPC分类号： G01N33/581 , C12Q1/68 , C12Q1/682 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
摘要翻译： 本文公开了信号共轭物的实施方案，使用信号传导共轭物的方法的实施方案以及包含信号传导共轭物的试剂盒的实施方案。 所公开的信号传导偶联物包含潜在反应性部分和显色部分，其可以进一步包含适于将潜在反应性部分偶联至显色部分的接头。 信号共轭物可以用于检测生物样品中的一种或多种目标物，并且能够直接共价沉积在目标物上或近端。 特别公开的使用信令共轭的方法的实施例包括复用方法。
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公开(公告)号：US20200319193A1
公开(公告)日：2020-10-08
申请号：US16856619
申请日：2020-04-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20180328932A1
公开(公告)日：2018-11-15
申请号：US16038389
申请日：2018-07-18
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , G01N33/542 , C12Q1/68 , G01N33/53 , C12Q1/682
CPC分类号： G01N33/581 , C12Q1/68 , C12Q1/682 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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公开(公告)号：US20220178934A1
公开(公告)日：2022-06-09
申请号：US17678389
申请日：2022-02-23
发明人： Nelson Alexander , William Day , Jerome W. Kosmeder, II , Mark Lefever , Larry Morrison , Anne M. Pedata , Stacey Stanislaw
IPC分类号： G01N33/58 , C12Q1/682 , C12Q1/68 , G01N33/53 , G01N33/542
摘要： Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.
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